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The map label for this gene is ywnE [H]

Identifier: 103487088

GI number: 103487088

Start: 1679910

End: 1681100

Strand: Direct

Name: ywnE [H]

Synonym: Sala_1603

Alternate gene names: 103487088

Gene position: 1679910-1681100 (Clockwise)

Preceding gene: 103487086

Following gene: 103487090

Centisome position: 50.22

GC content: 65.74

Gene sequence:

>1191_bases
ATGGCCGATCCCTGCCCCACTGCCGATCCGATGCAAGGGCTGACGGCGGACGTGGCGGATCATCGGATCGAGTTGATCTT
CGACGGCGGCGAGCGGATGACGCGCCTGCTCCGACTGATCGATCGGGCGGCGCACAGCATCGACCTCATCATCTATATAT
TTGAAGGCGACGCCGCAGGACTCAGCGTCCTGCGCGCGCTGACCGCCGCGGCGCGGCGCGGCGTGCGCGTGCGCGTGTTG
ATCGACAGCTTCGGGTCGGGCGACACGCCCGACGCGCTGTTCGCGCCGCTGCGCGAAGCGGGCGGCGGCGCCACCTTTTT
CTCGCGCCGCTGGCGTTCGTCCTATCTCATCCGCAATCACCAGAAGCTGATCCTGATCGACAATGCGGTTGCCATGACGG
GGGGCTTCAACATCGCCGACGATTATCTGAGCGCGCCGCGCAGCGATGGCTGGCTCGACATCGGCATGATCGTCGAGGGG
CCGAGCGTCGCGCGCGCGGCCGACTGGTTTGCCGAAATCCATGATTATACGGTCAGCAACGACGGTAAGCTCCTGATATT
GCGGCGGCTGATCCGCGAATGGCCGGTCGATGGCGGCGCAGTGTCATGGCTTGTCGGCGGGCCGACGCAGCGGCTTTCGC
CCTGGGCACGCGCCGTGCGCGCCGACCTGAACGATGCGCGTCAACTCGACATGGCAATGGCCTATTTTTCACCCGGCCAG
GGCCTGCTCCGCCGCCTCGGCCGCGTCGCGCAGCGCGGTCGCGCGCGCTTCATCATGGCGGGCAAGTCCGACAATGGCGC
CACGATCGGGGCCTCGCGCCTTCTCTATGGCTATCTGCTTCGCAAGGGCGCCGACGTGTGGGAATATCGGCGGTGCAAGC
TGCACATGAAGCTGATCGTCGTCGACGATGTGGTCTATATCGGCTCGGCCAATTTCGACGTGCGCAGCCTGTTCGTCAAT
GTCGAGCTGATGGTGCGGATCGCCGATGCCGGCTTTGCGGCGCAGATGCGGCGCTTTGTGGCGGGCCTCCAGCCCGATTG
CGACATCATCACCGCCGAGGCGCACAAGGCACGCGCAAGCTGGTGGACGCGGCTGCGCTGGACACTCGCCTGGTTCGTCG
TCGGCGTCGCCGATTATACGGTGTCGCGCAAACTCAACTTCGGCCTCGGTGACCCCGATCCCGATGTCTAG

Upstream 100 bases:

>100_bases
GGGGCATGGCGGCAAGCGCTGCGCGCTAGCAGGCTCGCCCCCGCCTGTCAACGAAAGCGCCGCCCTTGCGGCGCGCGCCA
ATGCCGTCCTATGAGCAGGC

Downstream 100 bases:

>100_bases
CCCCTAATCCTTCCAGGCCCAGAAGAGGAAACAGGGCGGCACATTCACCCACTCGAACGCATTGTCGATGCGGGCGAAAT
GCGCGGCAAGGAAATCGCGC

Product: phospholipase D/transphosphatidylase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MADPCPTADPMQGLTADVADHRIELIFDGGERMTRLLRLIDRAAHSIDLIIYIFEGDAAGLSVLRALTAAARRGVRVRVL
IDSFGSGDTPDALFAPLREAGGGATFFSRRWRSSYLIRNHQKLILIDNAVAMTGGFNIADDYLSAPRSDGWLDIGMIVEG
PSVARAADWFAEIHDYTVSNDGKLLILRRLIREWPVDGGAVSWLVGGPTQRLSPWARAVRADLNDARQLDMAMAYFSPGQ
GLLRRLGRVAQRGRARFIMAGKSDNGATIGASRLLYGYLLRKGADVWEYRRCKLHMKLIVVDDVVYIGSANFDVRSLFVN
VELMVRIADAGFAAQMRRFVAGLQPDCDIITAEAHKARASWWTRLRWTLAWFVVGVADYTVSRKLNFGLGDPDPDV

Sequences:

>Translated_396_residues
MADPCPTADPMQGLTADVADHRIELIFDGGERMTRLLRLIDRAAHSIDLIIYIFEGDAAGLSVLRALTAAARRGVRVRVL
IDSFGSGDTPDALFAPLREAGGGATFFSRRWRSSYLIRNHQKLILIDNAVAMTGGFNIADDYLSAPRSDGWLDIGMIVEG
PSVARAADWFAEIHDYTVSNDGKLLILRRLIREWPVDGGAVSWLVGGPTQRLSPWARAVRADLNDARQLDMAMAYFSPGQ
GLLRRLGRVAQRGRARFIMAGKSDNGATIGASRLLYGYLLRKGADVWEYRRCKLHMKLIVVDDVVYIGSANFDVRSLFVN
VELMVRIADAGFAAQMRRFVAGLQPDCDIITAEAHKARASWWTRLRWTLAWFVVGVADYTVSRKLNFGLGDPDPDV
>Mature_395_residues
ADPCPTADPMQGLTADVADHRIELIFDGGERMTRLLRLIDRAAHSIDLIIYIFEGDAAGLSVLRALTAAARRGVRVRVLI
DSFGSGDTPDALFAPLREAGGGATFFSRRWRSSYLIRNHQKLILIDNAVAMTGGFNIADDYLSAPRSDGWLDIGMIVEGP
SVARAADWFAEIHDYTVSNDGKLLILRRLIREWPVDGGAVSWLVGGPTQRLSPWARAVRADLNDARQLDMAMAYFSPGQG
LLRRLGRVAQRGRARFIMAGKSDNGATIGASRLLYGYLLRKGADVWEYRRCKLHMKLIVVDDVVYIGSANFDVRSLFVNV
ELMVRIADAGFAAQMRRFVAGLQPDCDIITAEAHKARASWWTRLRWTLAWFVVGVADYTVSRKLNFGLGDPDPDV

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=370, Percent_Identity=30.2702702702703, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787502, Length=339, Percent_Identity=28.023598820059, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081826, Length=421, Percent_Identity=26.3657957244656, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 43869; Mature: 43738

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPCPTADPMQGLTADVADHRIELIFDGGERMTRLLRLIDRAAHSIDLIIYIFEGDAAG
CCCCCCCCCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEEECCHHH
LSVLRALTAAARRGVRVRVLIDSFGSGDTPDALFAPLREAGGGATFFSRRWRSSYLIRNH
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHEECC
QKLILIDNAVAMTGGFNIADDYLSAPRSDGWLDIGMIVEGPSVARAADWFAEIHDYTVSN
CEEEEEECEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCEEECC
DGKLLILRRLIREWPVDGGAVSWLVGGPTQRLSPWARAVRADLNDARQLDMAMAYFSPGQ
CCCCHHHHHHHHHCCCCCCEEEEEECCCHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCH
GLLRRLGRVAQRGRARFIMAGKSDNGATIGASRLLYGYLLRKGADVWEYRRCKLHMKLIV
HHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHEEEEEEEEE
VDDVVYIGSANFDVRSLFVNVELMVRIADAGFAAQMRRFVAGLQPDCDIITAEAHKARAS
EEEEEEECCCCCCHHHEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCHHHHHHH
WWTRLRWTLAWFVVGVADYTVSRKLNFGLGDPDPDV
HHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
ADPCPTADPMQGLTADVADHRIELIFDGGERMTRLLRLIDRAAHSIDLIIYIFEGDAAG
CCCCCCCCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEEECCHHH
LSVLRALTAAARRGVRVRVLIDSFGSGDTPDALFAPLREAGGGATFFSRRWRSSYLIRNH
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHEECC
QKLILIDNAVAMTGGFNIADDYLSAPRSDGWLDIGMIVEGPSVARAADWFAEIHDYTVSN
CEEEEEECEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCEEECC
DGKLLILRRLIREWPVDGGAVSWLVGGPTQRLSPWARAVRADLNDARQLDMAMAYFSPGQ
CCCCHHHHHHHHHCCCCCCEEEEEECCCHHHCCHHHHHHHHCCHHHHHHHHHHHHCCCCH
GLLRRLGRVAQRGRARFIMAGKSDNGATIGASRLLYGYLLRKGADVWEYRRCKLHMKLIV
HHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHEEEEEEEEE
VDDVVYIGSANFDVRSLFVNVELMVRIADAGFAAQMRRFVAGLQPDCDIITAEAHKARAS
EEEEEEECCCCCCHHHEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCHHHHHHH
WWTRLRWTLAWFVVGVADYTVSRKLNFGLGDPDPDV
HHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]