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The map label for this gene is tilS [H]

Identifier: 103487083

GI number: 103487083

Start: 1674728

End: 1675582

Strand: Direct

Name: tilS [H]

Synonym: Sala_1598

Alternate gene names: 103487083

Gene position: 1674728-1675582 (Clockwise)

Preceding gene: 103487082

Following gene: 103487084

Centisome position: 50.06

GC content: 70.41

Gene sequence:

>855_bases
ATGGCGCTGCTCTGGATGATGGTCTCGCTGCTGCCCGGTCAGGTGTGGGCGGCGACGGTCGATCATGGCCTGCGCAAGGG
ATCGGACGATGAAGCGCGCATGGTCGCGGCCTTCTGCGCGCGCGAACATATCCCCCATTCGACACTGCGACCGCCGACGC
CGATCCGCGGATCGCTGCAGGCGGCGGCGCGCACCGAACGCTATCGTCTGCTCGAACAATGGCGCGAGGCCCATGCGCTC
GACCATATCGTCACCGCGCATCATGCCGACGATCAGCTCGAAACGATCGTCATGCGGCTCAACCGCGCGAGCGGCGTCGG
CGGGCTCGCCGCGATCCGCGCCACCAACGGCGCCGTGCTGCGCCCGCTCCTTGGCTGGCGACGCCGCGAACTGGTCGATG
TCGCGCTCGACAACGACCTGCCCTTCATCGACGATCCCTCGAACAGCGACGATCGCTTCGACCGCGCGCGCCTGCGTCAT
GCGCTGCGCTCGCAGACCGCGCTCGACCCCGTAGCCGCGGCGAAATCGGCGGCATGGCTCGCCGAGGCCGACGAGGCGCT
CGACTGGACGGTCGATCGGCTCATCGCGGACTGGCCCGACGCCTCCGACATTGCGGTGATCCGCGACGAAGCCTATCCAC
CCGAACTGTTCCGCCGCATCGTGGCCCATCGGCTGCGCGCCAATGCGCCGCAGCTGGCGCTGCGCGGCGCCGCGCTCGAC
GGCGTGATCGCCGCGATGCGCGCCGGGCGGCGCGCGATGGTCGGCGCCTTGCTGATCGACGCCGATCGCGGGCTCGCAGG
GACGATCTGGCGCATTTCCGCGGCGCCGCGCCGGAAGGCGCCCAAAAAGGCCTGA

Upstream 100 bases:

>100_bases
CGACGTCGCGGGGCGGCTCGCCGGCGATCTCGCGGCGCTGCTTGGCGTCGGCTGGCACCGGCTGCATTATGGCGTCGCGG
TATCGGGCGGCCCCGACAGC

Downstream 100 bases:

>100_bases
TTGCCTCATTGTCATTTAACCCGATTATCCTATCTTGTCGGGTAGGAGCGCGCATTGCGCCGTTGAGGAAAGAACTCCGA
ATGCAGGACGACAAGGAACC

Product: tRNA(Ile)-lysidine synthetase-like protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MALLWMMVSLLPGQVWAATVDHGLRKGSDDEARMVAAFCAREHIPHSTLRPPTPIRGSLQAAARTERYRLLEQWREAHAL
DHIVTAHHADDQLETIVMRLNRASGVGGLAAIRATNGAVLRPLLGWRRRELVDVALDNDLPFIDDPSNSDDRFDRARLRH
ALRSQTALDPVAAAKSAAWLAEADEALDWTVDRLIADWPDASDIAVIRDEAYPPELFRRIVAHRLRANAPQLALRGAALD
GVIAAMRAGRRAMVGALLIDADRGLAGTIWRISAAPRRKAPKKA

Sequences:

>Translated_284_residues
MALLWMMVSLLPGQVWAATVDHGLRKGSDDEARMVAAFCAREHIPHSTLRPPTPIRGSLQAAARTERYRLLEQWREAHAL
DHIVTAHHADDQLETIVMRLNRASGVGGLAAIRATNGAVLRPLLGWRRRELVDVALDNDLPFIDDPSNSDDRFDRARLRH
ALRSQTALDPVAAAKSAAWLAEADEALDWTVDRLIADWPDASDIAVIRDEAYPPELFRRIVAHRLRANAPQLALRGAALD
GVIAAMRAGRRAMVGALLIDADRGLAGTIWRISAAPRRKAPKKA
>Mature_283_residues
ALLWMMVSLLPGQVWAATVDHGLRKGSDDEARMVAAFCAREHIPHSTLRPPTPIRGSLQAAARTERYRLLEQWREAHALD
HIVTAHHADDQLETIVMRLNRASGVGGLAAIRATNGAVLRPLLGWRRRELVDVALDNDLPFIDDPSNSDDRFDRARLRHA
LRSQTALDPVAAAKSAAWLAEADEALDWTVDRLIADWPDASDIAVIRDEAYPPELFRRIVAHRLRANAPQLALRGAALDG
VIAAMRAGRRAMVGALLIDADRGLAGTIWRISAAPRRKAPKKA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=148, Percent_Identity=35.8108108108108, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 31305; Mature: 31174

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLWMMVSLLPGQVWAATVDHGLRKGSDDEARMVAAFCAREHIPHSTLRPPTPIRGSLQ
CHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
AAARTERYRLLEQWREAHALDHIVTAHHADDQLETIVMRLNRASGVGGLAAIRATNGAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHH
RPLLGWRRRELVDVALDNDLPFIDDPSNSDDRFDRARLRHALRSQTALDPVAAAKSAAWL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEADEALDWTVDRLIADWPDASDIAVIRDEAYPPELFRRIVAHRLRANAPQLALRGAALD
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
GVIAAMRAGRRAMVGALLIDADRGLAGTIWRISAAPRRKAPKKA
HHHHHHHCCHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ALLWMMVSLLPGQVWAATVDHGLRKGSDDEARMVAAFCAREHIPHSTLRPPTPIRGSLQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
AAARTERYRLLEQWREAHALDHIVTAHHADDQLETIVMRLNRASGVGGLAAIRATNGAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHH
RPLLGWRRRELVDVALDNDLPFIDDPSNSDDRFDRARLRHALRSQTALDPVAAAKSAAWL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEADEALDWTVDRLIADWPDASDIAVIRDEAYPPELFRRIVAHRLRANAPQLALRGAALD
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
GVIAAMRAGRRAMVGALLIDADRGLAGTIWRISAAPRRKAPKKA
HHHHHHHCCHHHHHHHHEEECCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA