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The map label for this gene is nylB' [H]

Identifier: 103487029

GI number: 103487029

Start: 1610318

End: 1611487

Strand: Reverse

Name: nylB' [H]

Synonym: Sala_1544

Alternate gene names: 103487029

Gene position: 1611487-1610318 (Counterclockwise)

Preceding gene: 103487030

Following gene: 103487025

Centisome position: 48.17

GC content: 62.74

Gene sequence:

>1170_bases
ATGCGACGATTCCTGGCGGCGGTGCTGTTCGGCGCCGCGGCGGCGTGCAGTGTGCCCGCGACGACGCAAACCACCGATCA
ACTGCCAAAGGATCTGAACGTGCTTTTCTGGACCCAGGACCAACGCGACCGCGCCTTTCGCACGATGGAAACGGTGCCCA
GGGTCGTCGTGAACCCGATCGAGGCGGGCGATACCGCCTATCCCTTGCCGCAAGGCACGCCGATCGACTTTGGGCTCGAT
GTCGATGCCCATATGGCGAAGCAGCGCAACGCGGGGCTGATCATCGTACAGGACGGCAAGATCCGGCTCGAAAAATATGC
GCTCGGCCATGATGCGGCGGGCCGCTGGACGAGCTTTTCGGTCGCCAAGAGTTTCACCTCGACGCTCGTGGGGGCGGCGG
TGAAGGATGGCTATATCAAGAGCCTCGACGACAAGGTCGCCGCCTATATCCCCGGCCTGAAGGGATCGGCCTATGACGAT
GTCAGCGTGCGCCAGCTGCTGACGATGACTTCGGGCGTCAAATGGAACGAGGATTATACCGATCCCAAGTCCGACGTCGC
GCAGTTCAACCTGCAGAAACCGGTTGCGGGCGAAGATATAACGGTCAGCTATATGAAGGGCTTGCCGCGCGAAGCGCCTG
CGGGATCGAAATGGGTGTACAAGACCGGCGAGACCAATCTGATCGGGGTGCTGGTATCGAGCGCGACGGGCAAGACGCTG
TCGCAATATCTGTCGGAGAAAATCTGGAAGCCGTTCGGCATGGAGCAGGATGCGGCATGGATGCTCGGGCCGACGGGGCA
CGAGATCAGCGGCTGCTGCATGTCGGCAAGCCTCAGGGATTATGCACGTTTCGGCCAGTTCATCCTGAACGGCGGTGTGG
CGGGCAGCGTGAACGTGCTGCCCGACGACTGGCTGGCCTCGGCGACGACCAAGCAGGCCGAGATCGGCGTGCCGGGCCGC
GGCTATGGCTATCAATGGTGGACGAATGACGATGGCAGCTTTGCCGCGCAGGGCATTTTCGGGCAGGGCATCTTTATCGA
TCCGAAGCGCAAGCTGGTGATCGCGTCGAACGGCAACTGGCCGACCGCGACCGACCCCGAAGGCGTCGGCGCAGCGCGCG
AGGCCTTTTACAAGGCCGTGCAGGCGGCGGTGGACCGCGAGGCGAAATAG

Upstream 100 bases:

>100_bases
TATTATCTGCAATATTGCGAGGGAGGCTTTCGCGGCGGCGGGATCGACGTCGCGCAGGTCACACTTGAAAAACCGGCTTG
AGAACAGGGGAGGAAACAAG

Downstream 100 bases:

>100_bases
GAAAAGCGTCGCCCCCGCGAAGGCGGGGACCGCCGGAGATTTGCGCGGCCATGCTGAGGAAGGCCGCGCGCGGCCCCCGC
CTTCGCGGGGGCGACGATTA

Product: beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRRFLAAVLFGAAAACSVPATTQTTDQLPKDLNVLFWTQDQRDRAFRTMETVPRVVVNPIEAGDTAYPLPQGTPIDFGLD
VDAHMAKQRNAGLIIVQDGKIRLEKYALGHDAAGRWTSFSVAKSFTSTLVGAAVKDGYIKSLDDKVAAYIPGLKGSAYDD
VSVRQLLTMTSGVKWNEDYTDPKSDVAQFNLQKPVAGEDITVSYMKGLPREAPAGSKWVYKTGETNLIGVLVSSATGKTL
SQYLSEKIWKPFGMEQDAAWMLGPTGHEISGCCMSASLRDYARFGQFILNGGVAGSVNVLPDDWLASATTKQAEIGVPGR
GYGYQWWTNDDGSFAAQGIFGQGIFIDPKRKLVIASNGNWPTATDPEGVGAAREAFYKAVQAAVDREAK

Sequences:

>Translated_389_residues
MRRFLAAVLFGAAAACSVPATTQTTDQLPKDLNVLFWTQDQRDRAFRTMETVPRVVVNPIEAGDTAYPLPQGTPIDFGLD
VDAHMAKQRNAGLIIVQDGKIRLEKYALGHDAAGRWTSFSVAKSFTSTLVGAAVKDGYIKSLDDKVAAYIPGLKGSAYDD
VSVRQLLTMTSGVKWNEDYTDPKSDVAQFNLQKPVAGEDITVSYMKGLPREAPAGSKWVYKTGETNLIGVLVSSATGKTL
SQYLSEKIWKPFGMEQDAAWMLGPTGHEISGCCMSASLRDYARFGQFILNGGVAGSVNVLPDDWLASATTKQAEIGVPGR
GYGYQWWTNDDGSFAAQGIFGQGIFIDPKRKLVIASNGNWPTATDPEGVGAAREAFYKAVQAAVDREAK
>Mature_389_residues
MRRFLAAVLFGAAAACSVPATTQTTDQLPKDLNVLFWTQDQRDRAFRTMETVPRVVVNPIEAGDTAYPLPQGTPIDFGLD
VDAHMAKQRNAGLIIVQDGKIRLEKYALGHDAAGRWTSFSVAKSFTSTLVGAAVKDGYIKSLDDKVAAYIPGLKGSAYDD
VSVRQLLTMTSGVKWNEDYTDPKSDVAQFNLQKPVAGEDITVSYMKGLPREAPAGSKWVYKTGETNLIGVLVSSATGKTL
SQYLSEKIWKPFGMEQDAAWMLGPTGHEISGCCMSASLRDYARFGQFILNGGVAGSVNVLPDDWLASATTKQAEIGVPGR
GYGYQWWTNDDGSFAAQGIFGQGIFIDPKRKLVIASNGNWPTATDPEGVGAAREAFYKAVQAAVDREAK

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 42042; Mature: 42042

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFLAAVLFGAAAACSVPATTQTTDQLPKDLNVLFWTQDQRDRAFRTMETVPRVVVNPI
CHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
EAGDTAYPLPQGTPIDFGLDVDAHMAKQRNAGLIIVQDGKIRLEKYALGHDAAGRWTSFS
CCCCCCCCCCCCCCCEECCCCHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHH
VAKSFTSTLVGAAVKDGYIKSLDDKVAAYIPGLKGSAYDDVSVRQLLTMTSGVKWNEDYT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
DPKSDVAQFNLQKPVAGEDITVSYMKGLPREAPAGSKWVYKTGETNLIGVLVSSATGKTL
CCHHHHHHCCCCCCCCCCCEEEHHHHCCCCCCCCCCCEEEECCCCEEEEEEEECCCCHHH
SQYLSEKIWKPFGMEQDAAWMLGPTGHEISGCCMSASLRDYARFGQFILNGGVAGSVNVL
HHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
PDDWLASATTKQAEIGVPGRGYGYQWWTNDDGSFAAQGIFGQGIFIDPKRKLVIASNGNW
CCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCEEECCCCEEEEEECCCC
PTATDPEGVGAAREAFYKAVQAAVDREAK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRFLAAVLFGAAAACSVPATTQTTDQLPKDLNVLFWTQDQRDRAFRTMETVPRVVVNPI
CHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
EAGDTAYPLPQGTPIDFGLDVDAHMAKQRNAGLIIVQDGKIRLEKYALGHDAAGRWTSFS
CCCCCCCCCCCCCCCEECCCCHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHH
VAKSFTSTLVGAAVKDGYIKSLDDKVAAYIPGLKGSAYDDVSVRQLLTMTSGVKWNEDYT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
DPKSDVAQFNLQKPVAGEDITVSYMKGLPREAPAGSKWVYKTGETNLIGVLVSSATGKTL
CCHHHHHHCCCCCCCCCCCEEEHHHHCCCCCCCCCCCEEEECCCCEEEEEEEECCCCHHH
SQYLSEKIWKPFGMEQDAAWMLGPTGHEISGCCMSASLRDYARFGQFILNGGVAGSVNVL
HHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
PDDWLASATTKQAEIGVPGRGYGYQWWTNDDGSFAAQGIFGQGIFIDPKRKLVIASNGNW
CCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCEEECCCCEEEEEECCCC
PTATDPEGVGAAREAFYKAVQAAVDREAK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]