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The map label for this gene is hcaR [H]

Identifier: 103487013

GI number: 103487013

Start: 1594792

End: 1595694

Strand: Reverse

Name: hcaR [H]

Synonym: Sala_1528

Alternate gene names: 103487013

Gene position: 1595694-1594792 (Counterclockwise)

Preceding gene: 103487021

Following gene: 103487012

Centisome position: 47.7

GC content: 67.77

Gene sequence:

>903_bases
ATGATTAAACGCGCGCATATCCGGCAATTTCTCGCTGTCGTCGATGCAGGCAGCTTCACGCAGGCGGCTTTGCGCATCCG
TGTCACCCAGCCAGCGCTGTCGGCGGGGATCGCCGAACTGGAAAGGCTGGTCGGGACACCGTTGTTCCTGCGCAACCGAC
GGCAGATACGCCTGACCGAAGCGGGCGGCCGTTTCCTGCCGATCGCGCGCGACCTCGACCGCGGATTTCGCGCGGCGGAC
GATTTCGGCCGCACCGGCGAACAGCAGGCGGCCGAGCTGAAGCTTGGTGTGATCCGGTCGCTGTCCGGCGAACTGTTGCA
GGCGATCGTCGCGGCGCTGCGTCCGAGTTTTGCGATCGAGATCGTCGAAAACGGCGATGCCGAGCTTCGCGCCGCGCTGG
GCAGCGCGCGCATCCATATGGCGATCGGCGGGCTACGTCCCGGCGAGCGCGGGGAGCATGTCACGCCGCTCTATGACGAA
CCGTTGACAATGTTCGTTGCCGCCGACCATCCGCTCGTCGGTCGCATCGAAGTCACGCCCGACGAGCTCGCCGCCGAAAC
GATGATCGCGCGGCGTTCGTGCGAGTTTCTGGCCGCCACCAGCCGCTTTTTCACGCGCCACGGCGTACGGCCGCGTTTCG
CGCTGCGCAGCGAAAGCGATGAGCGGTGCCTGCGGATGGTCGCCGCGGGCATCGGCATCACCACCGCGCCGGTGTCGCTG
GCGATCGACGGGATCGTGCCGCTCAAGGTCGCGGGCTATGATTTTCGCCGCGAACTCGGGCTGATCGCCGATCCCGCCTG
GGCGGCGCTGCCCGACGTGGCGCCGCGGCTGGCGCACGCACGCGAGATGATCGGCGCGATCGCCGCCGAGTGGCGGCAGG
CAAAGGTCGATCCGGCCTTGTGA

Upstream 100 bases:

>100_bases
GGAGTACCTGTCGATCGAGGGCCGGACGGGAGGGGATAGTGCCCCAAAAACCCAGCATATGGAAATTGAAAGAGATCGCA
GTTCGGCATAAACAAAATCT

Downstream 100 bases:

>100_bases
TGGCCAGCCTCCCAAGTCTGGCTTGCAGCGCCGAAAGGCCATACAGCTTGATCGCTTCATGGAGGGGCGGCAAGGGCAGG
GCATGACCGATACGCTGACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIKRAHIRQFLAVVDAGSFTQAALRIRVTQPALSAGIAELERLVGTPLFLRNRRQIRLTEAGGRFLPIARDLDRGFRAAD
DFGRTGEQQAAELKLGVIRSLSGELLQAIVAALRPSFAIEIVENGDAELRAALGSARIHMAIGGLRPGERGEHVTPLYDE
PLTMFVAADHPLVGRIEVTPDELAAETMIARRSCEFLAATSRFFTRHGVRPRFALRSESDERCLRMVAAGIGITTAPVSL
AIDGIVPLKVAGYDFRRELGLIADPAWAALPDVAPRLAHAREMIGAIAAEWRQAKVDPAL

Sequences:

>Translated_300_residues
MIKRAHIRQFLAVVDAGSFTQAALRIRVTQPALSAGIAELERLVGTPLFLRNRRQIRLTEAGGRFLPIARDLDRGFRAAD
DFGRTGEQQAAELKLGVIRSLSGELLQAIVAALRPSFAIEIVENGDAELRAALGSARIHMAIGGLRPGERGEHVTPLYDE
PLTMFVAADHPLVGRIEVTPDELAAETMIARRSCEFLAATSRFFTRHGVRPRFALRSESDERCLRMVAAGIGITTAPVSL
AIDGIVPLKVAGYDFRRELGLIADPAWAALPDVAPRLAHAREMIGAIAAEWRQAKVDPAL
>Mature_300_residues
MIKRAHIRQFLAVVDAGSFTQAALRIRVTQPALSAGIAELERLVGTPLFLRNRRQIRLTEAGGRFLPIARDLDRGFRAAD
DFGRTGEQQAAELKLGVIRSLSGELLQAIVAALRPSFAIEIVENGDAELRAALGSARIHMAIGGLRPGERGEHVTPLYDE
PLTMFVAADHPLVGRIEVTPDELAAETMIARRSCEFLAATSRFFTRHGVRPRFALRSESDERCLRMVAAGIGITTAPVSL
AIDGIVPLKVAGYDFRRELGLIADPAWAALPDVAPRLAHAREMIGAIAAEWRQAKVDPAL

Specific function: Transcriptional activator of the hca operon for 3- phenylpropionic acid catabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=240, Percent_Identity=27.5, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790399, Length=270, Percent_Identity=28.8888888888889, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI157672245, Length=263, Percent_Identity=29.277566539924, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789204, Length=245, Percent_Identity=29.7959183673469, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=266, Percent_Identity=26.6917293233083, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI145693105, Length=277, Percent_Identity=26.7148014440433, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787879, Length=255, Percent_Identity=28.2352941176471, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787601, Length=287, Percent_Identity=24.0418118466899, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081904, Length=185, Percent_Identity=29.1891891891892, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32619; Mature: 32619

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRAHIRQFLAVVDAGSFTQAALRIRVTQPALSAGIAELERLVGTPLFLRNRRQIRLTE
CCCHHHHHHHHHHHCCCCHHEEEEEEEEECHHHHHHHHHHHHHHCCCHHCCCCCEEEEEC
AGGRFLPIARDLDRGFRAADDFGRTGEQQAAELKLGVIRSLSGELLQAIVAALRPSFAIE
CCCCEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IVENGDAELRAALGSARIHMAIGGLRPGERGEHVTPLYDEPLTMFVAADHPLVGRIEVTP
EEECCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECH
DELAAETMIARRSCEFLAATSRFFTRHGVRPRFALRSESDERCLRMVAAGIGITTAPVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHCCCCEECCHHE
AIDGIVPLKVAGYDFRRELGLIADPAWAALPDVAPRLAHAREMIGAIAAEWRQAKVDPAL
EECCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIKRAHIRQFLAVVDAGSFTQAALRIRVTQPALSAGIAELERLVGTPLFLRNRRQIRLTE
CCCHHHHHHHHHHHCCCCHHEEEEEEEEECHHHHHHHHHHHHHHCCCHHCCCCCEEEEEC
AGGRFLPIARDLDRGFRAADDFGRTGEQQAAELKLGVIRSLSGELLQAIVAALRPSFAIE
CCCCEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IVENGDAELRAALGSARIHMAIGGLRPGERGEHVTPLYDEPLTMFVAADHPLVGRIEVTP
EEECCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECH
DELAAETMIARRSCEFLAATSRFFTRHGVRPRFALRSESDERCLRMVAAGIGITTAPVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHCCCCEECCHHE
AIDGIVPLKVAGYDFRRELGLIADPAWAALPDVAPRLAHAREMIGAIAAEWRQAKVDPAL
EECCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 9603882 [H]