Click here to switch to the map view.

The map label for this gene is cysD [H]

Identifier: 103487012

GI number: 103487012

Start: 1593804

End: 1594709

Strand: Reverse

Name: cysD [H]

Synonym: Sala_1527

Alternate gene names: 103487012

Gene position: 1594709-1593804 (Counterclockwise)

Preceding gene: 103487013

Following gene: 103487011

Centisome position: 47.67

GC content: 63.02

Gene sequence:

>906_bases
ATGACCGATACGCTGACCCATCTCGACCGGCTGGAGGCCGAGAGCATCCATATCATGCGCGAAGTGATGGCCGACGCGGC
GAAGCCCGTGATGCTGTACAGCGTGGGCAAGGACAGCGCGGTGATGCTGCACCTCGCGCGCAAGGCCTTTTATCCCTCGC
CGCCGCCCTTCCCGCTGCTGCACGTCGACACGACGTGGAAGTTCCGGGCGATGTACGAGCTGCGCGACCGGATGGCGGCC
GAGAGCGGCATGGAACTCATCGTCCATCAGAACCCCGAAGCGAAAGCGCGGGGCATCAATCCGTTCGACCATGGCCCGCT
GCATACCGACATGTGGAAGACCGAGGGGCTCAAGCAGGCGCTCGATCTCCACGGCTTCGATGTCGCTTTCGGCGGCGCGC
GGCGCGACGAGGAAAAGAGCCGTGCGAAGGAACGTATCTTTTCCTTCCGCACCGCGAGCCACGGCTGGGATCCCAAGAAG
CAGCGGCCCGAACTGTGGAATCTCTACAACGCGCGCAAGGCGAAGGGCGAGAGCATCCGTGTGTTCCCGATCTCCAACTG
GACCGAGCTCGACATCTGGCAATATATCGCGCGCGAAAATATCCCGATCGTGCCGCTCTATTTTGCCGCGCCGCGCCCGA
CGGTGGAGCGCGACGGCCTTTTGCTGATGGTCGATGACGATCGCTTTCCGCTGCGGCCCGGTGAAGTGCCGACCCTCCGC
TCGGTGCGCTTCCGCACGCTGGGTTGTTATCCGCTCACCGGCGCGGTCGAGAGCGAGGCGAAAACATTGTCCGAGGTCAT
TCAGGAGATGCTGCTGACGACGACCAGCGAACGCCAGGGGCGCATCATCGACAAGGACAGCGGTGATGCCAGCATGGAGA
AGAAGAAGCAGGAGGGCTACTTCTGA

Upstream 100 bases:

>100_bases
GTCGATCCGGCCTTGTGATGGCCAGCCTCCCAAGTCTGGCTTGCAGCGCCGAAAGGCCATACAGCTTGATCGCTTCATGG
AGGGGCGGCAAGGGCAGGGC

Downstream 100 bases:

>100_bases
TGACCGATCCCATCTACGTCACCGACGCGCTGATCGCCCAGGACATCGACGCCTATCTCGCGGGTCACGAGAAGAAGTCG
CTGCTGCGCTTCATCACCTG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTDTLTHLDRLEAESIHIMREVMADAAKPVMLYSVGKDSAVMLHLARKAFYPSPPPFPLLHVDTTWKFRAMYELRDRMAA
ESGMELIVHQNPEAKARGINPFDHGPLHTDMWKTEGLKQALDLHGFDVAFGGARRDEEKSRAKERIFSFRTASHGWDPKK
QRPELWNLYNARKAKGESIRVFPISNWTELDIWQYIARENIPIVPLYFAAPRPTVERDGLLLMVDDDRFPLRPGEVPTLR
SVRFRTLGCYPLTGAVESEAKTLSEVIQEMLLTTTSERQGRIIDKDSGDASMEKKKQEGYF

Sequences:

>Translated_301_residues
MTDTLTHLDRLEAESIHIMREVMADAAKPVMLYSVGKDSAVMLHLARKAFYPSPPPFPLLHVDTTWKFRAMYELRDRMAA
ESGMELIVHQNPEAKARGINPFDHGPLHTDMWKTEGLKQALDLHGFDVAFGGARRDEEKSRAKERIFSFRTASHGWDPKK
QRPELWNLYNARKAKGESIRVFPISNWTELDIWQYIARENIPIVPLYFAAPRPTVERDGLLLMVDDDRFPLRPGEVPTLR
SVRFRTLGCYPLTGAVESEAKTLSEVIQEMLLTTTSERQGRIIDKDSGDASMEKKKQEGYF
>Mature_300_residues
TDTLTHLDRLEAESIHIMREVMADAAKPVMLYSVGKDSAVMLHLARKAFYPSPPPFPLLHVDTTWKFRAMYELRDRMAAE
SGMELIVHQNPEAKARGINPFDHGPLHTDMWKTEGLKQALDLHGFDVAFGGARRDEEKSRAKERIFSFRTASHGWDPKKQ
RPELWNLYNARKAKGESIRVFPISNWTELDIWQYIARENIPIVPLYFAAPRPTVERDGLLLMVDDDRFPLRPGEVPTLRS
VRFRTLGCYPLTGAVESEAKTLSEVIQEMLLTTTSERQGRIIDKDSGDASMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=298, Percent_Identity=71.4765100671141, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34501; Mature: 34370

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTLTHLDRLEAESIHIMREVMADAAKPVMLYSVGKDSAVMLHLARKAFYPSPPPFPLL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEE
HVDTTWKFRAMYELRDRMAAESGMELIVHQNPEAKARGINPFDHGPLHTDMWKTEGLKQA
EECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LDLHGFDVAFGGARRDEEKSRAKERIFSFRTASHGWDPKKQRPELWNLYNARKAKGESIR
HHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
VFPISNWTELDIWQYIARENIPIVPLYFAAPRPTVERDGLLLMVDDDRFPLRPGEVPTLR
EEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCHH
SVRFRTLGCYPLTGAVESEAKTLSEVIQEMLLTTTSERQGRIIDKDSGDASMEKKKQEGY
HHHEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHCCC
F
C
>Mature Secondary Structure 
TDTLTHLDRLEAESIHIMREVMADAAKPVMLYSVGKDSAVMLHLARKAFYPSPPPFPLL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEE
HVDTTWKFRAMYELRDRMAAESGMELIVHQNPEAKARGINPFDHGPLHTDMWKTEGLKQA
EECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LDLHGFDVAFGGARRDEEKSRAKERIFSFRTASHGWDPKKQRPELWNLYNARKAKGESIR
HHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
VFPISNWTELDIWQYIARENIPIVPLYFAAPRPTVERDGLLLMVDDDRFPLRPGEVPTLR
EEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCHH
SVRFRTLGCYPLTGAVESEAKTLSEVIQEMLLTTTSERQGRIIDKDSGDASMEKKKQEGY
HHHEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]