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The map label for this gene is ksgA

Identifier: 103486963

GI number: 103486963

Start: 1538110

End: 1538925

Strand: Direct

Name: ksgA

Synonym: Sala_1478

Alternate gene names: 103486963

Gene position: 1538110-1538925 (Clockwise)

Preceding gene: 103486962

Following gene: 103486965

Centisome position: 45.98

GC content: 69.49

Gene sequence:

>816_bases
TTGCCGCCACTGCGCGAGACGGTGCGCGTCCACGGTCTGTCGGCGAGCAAGGCGCTGGGACAGAACTTTCTCTTCGACGA
ACAGTTGCTCGATCGCATCGCCGCGATTCCCGGTGACCTTGATGGCGCAACGGTGTTCGAGGTCGGCCCCGGTCCGGGCG
GGCTGACGCGCGCGCTTCTGCGCACGGGGGCCAGGGTGATCGCTGTCGAGCGCGACGAGCGCTGCCTGCCGCTGCTGGCC
GACCTCGCCGAGGCGTTTCCGGGGCAGCTGACGGTGATCGCCGACGATGCGATGGCGGTCGATGTCGATGCGCTGACCGG
CGGCGATCCTTATCATATCGTCGCCAACCTGCCCTATAATGTCGGGACGGCGCTGTTCACGCGCTGGCTCGAACCCGCGG
CGTGGCCGCCGCGCTGGCTGTCGCTGACGCTGATGTTCCAGCTCGAGGTCGCCGAACGCATCGTCGCGCCGGTCGGCACG
AGTGCCTATGGCCGCCTCGCGGTGCTCGCGCAGTGGCGCGCCAGGGCGAGGATCGCGATGAAAGTGCATCGCTCGGCCTT
CACCCCGCCGCCCAAGGTCATGTCGGCCATCGTCCAGCTGACGCCCGCCGACCAACCCCCAGGCGTCGATCCGCGCATCC
TGTCTCGGCTGACCGAAAAAGGCTTTGGCCAGCGCCGCAAGATGTTGCGGCAGAGCCTGAAGGGGATCGACGGCGCGGTC
GCCGCCGCCGAAGCGCTGGGAATCGACCCGACGCGGCGCGCCGAAACGGTCAGCGTCGCCGAATGGGTCGCGCTGGCGCG
CGCACTGGGCCGCTGA

Upstream 100 bases:

>100_bases
CCGGTCCGACGATTGCCGCGATCGCGATGGCCGCGCGCATGGCCGCGGCGCGCGAACGCAGCTGCGCGCCGGCAAAGTGA
CGATCCAGCCCAGGACGCCG

Downstream 100 bases:

>100_bases
CAGCATCCGCCTCGGCCTTGTGGCAGCCCGAATGTCCGCCATCGGCGGCGCCCGCGTTGCAAGCATGGAGCGGCGCTCTC
GACGGCAAAACCACTCCACC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPPLRETVRVHGLSASKALGQNFLFDEQLLDRIAAIPGDLDGATVFEVGPGPGGLTRALLRTGARVIAVERDERCLPLLA
DLAEAFPGQLTVIADDAMAVDVDALTGGDPYHIVANLPYNVGTALFTRWLEPAAWPPRWLSLTLMFQLEVAERIVAPVGT
SAYGRLAVLAQWRARARIAMKVHRSAFTPPPKVMSAIVQLTPADQPPGVDPRILSRLTEKGFGQRRKMLRQSLKGIDGAV
AAAEALGIDPTRRAETVSVAEWVALARALGR

Sequences:

>Translated_271_residues
MPPLRETVRVHGLSASKALGQNFLFDEQLLDRIAAIPGDLDGATVFEVGPGPGGLTRALLRTGARVIAVERDERCLPLLA
DLAEAFPGQLTVIADDAMAVDVDALTGGDPYHIVANLPYNVGTALFTRWLEPAAWPPRWLSLTLMFQLEVAERIVAPVGT
SAYGRLAVLAQWRARARIAMKVHRSAFTPPPKVMSAIVQLTPADQPPGVDPRILSRLTEKGFGQRRKMLRQSLKGIDGAV
AAAEALGIDPTRRAETVSVAEWVALARALGR
>Mature_270_residues
PPLRETVRVHGLSASKALGQNFLFDEQLLDRIAAIPGDLDGATVFEVGPGPGGLTRALLRTGARVIAVERDERCLPLLAD
LAEAFPGQLTVIADDAMAVDVDALTGGDPYHIVANLPYNVGTALFTRWLEPAAWPPRWLSLTLMFQLEVAERIVAPVGTS
AYGRLAVLAQWRARARIAMKVHRSAFTPPPKVMSAIVQLTPADQPPGVDPRILSRLTEKGFGQRRKMLRQSLKGIDGAVA
AAEALGIDPTRRAETVSVAEWVALARALGR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=290, Percent_Identity=34.4827586206897, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI7657198, Length=232, Percent_Identity=31.8965517241379, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1786236, Length=274, Percent_Identity=39.051094890511, Blast_Score=151, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25141369, Length=299, Percent_Identity=27.7591973244147, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25146882, Length=208, Percent_Identity=34.6153846153846, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=33.9622641509434, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21357273, Length=244, Percent_Identity=35.655737704918, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21358017, Length=239, Percent_Identity=32.2175732217573, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_SPHAL (Q1GT31)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616524.1
- ProteinModelPortal:   Q1GT31
- SMR:   Q1GT31
- GeneID:   4081250
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1478
- NMPDR:   fig|317655.9.peg.1411
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   SALA317655:SALA_1478-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29189; Mature: 29058

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 22-22 BINDING 24-24 BINDING 49-49 BINDING 70-70 BINDING 96-96 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLRETVRVHGLSASKALGQNFLFDEQLLDRIAAIPGDLDGATVFEVGPGPGGLTRALL
CCCHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
RTGARVIAVERDERCLPLLADLAEAFPGQLTVIADDAMAVDVDALTGGDPYHIVANLPYN
HCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCCEEEEEECCCH
VGTALFTRWLEPAAWPPRWLSLTLMFQLEVAERIVAPVGTSAYGRLAVLAQWRARARIAM
HHHHHHHHHHCCCCCCCHHHEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KVHRSAFTPPPKVMSAIVQLTPADQPPGVDPRILSRLTEKGFGQRRKMLRQSLKGIDGAV
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHH
AAAEALGIDPTRRAETVSVAEWVALARALGR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PPLRETVRVHGLSASKALGQNFLFDEQLLDRIAAIPGDLDGATVFEVGPGPGGLTRALL
CCHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
RTGARVIAVERDERCLPLLADLAEAFPGQLTVIADDAMAVDVDALTGGDPYHIVANLPYN
HCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCCEEEEEECCCH
VGTALFTRWLEPAAWPPRWLSLTLMFQLEVAERIVAPVGTSAYGRLAVLAQWRARARIAM
HHHHHHHHHHCCCCCCCHHHEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KVHRSAFTPPPKVMSAIVQLTPADQPPGVDPRILSRLTEKGFGQRRKMLRQSLKGIDGAV
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHH
AAAEALGIDPTRRAETVSVAEWVALARALGR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA