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The map label for this gene is 103486869

Identifier: 103486869

GI number: 103486869

Start: 1438928

End: 1439830

Strand: Direct

Name: 103486869

Synonym: Sala_1383

Alternate gene names: NA

Gene position: 1438928-1439830 (Clockwise)

Preceding gene: 103486865

Following gene: 103486879

Centisome position: 43.02

GC content: 64.01

Gene sequence:

>903_bases
GTGTGGGCTTTTGCACTGGCAAGATACGCGCCCTGCCCTATATCCGGCCCCATGACCCGCACCATCACCGTTGCCGCGTT
GCAGCTTGCCCTGCCCGGCCCCGTCGAACCGAACATCAAGGCTGTCACCGCGCTTGTCGAGGCAGCGGCGGCAAGGGGCG
CGCAGATCATCCTGCCACCCGAACTCTTCGAAGGCCCCTATTTCTGCCAGGTCGAGGAAGAGGAGTTGTTCGCCACCGCA
CGGCCGACGGCCGAGCATCCGAGCGTCGTCGCGATGCAGGCGCTCGCCGCGAAGTGCAAGGTCGCGATCCCGACGAGCTT
TTTCGAACGCGACGGGCATCATTATTACAATACGCTGGCGATGATCGGTCCCGACGGCGGGATCATGGGCACCTACCGCA
AGTCGCACATCCCCGACGGGCCGGGTTACGAAGAAAAATATTATTTCCGCCCCGGCAACACCGGCTTCAAGATCTGGGAG
GTCTTCGACACCCGCATCGGCGTCGGCGTCTGCTGGGACCAATGGTATCCCGAATGCGCGCGCGCGATGGCGTTGATGGG
CGCCGAACTGCTCTTCTACCCGACGGCGATCGGCAGCGAACCCTATGACGCCGACCTCGACACCAGCCGTATGTGGCGCC
GCGCGATGCAGGGTCATGCCGTGTCGAACTGTATGCCCGTCATCGCAGCGAACCGCATCGGAACGGAGGGCGACGCGCGT
TTCTATGGCCACAGCTTCATCGCCGACGAATGGGGCGATCTGACGCAGGCGTTTGGCGCCAGCGAAACGGGTGCGCTCGT
CGAGACGATCGACCTCGACCGCGCGGCAAAGCATCGTGCGGGGATGGGGTTTTTCCGCGACCGGCGACCACAGCTTTACG
GGCGGCTCGTCGAAGACGTCTGA

Upstream 100 bases:

>100_bases
TTTGTCGACATGTTGAAAGAACCGCGGCGAAGGCAGGCACAGCCGGGGCATGTAGGACAGTGCTTTTTTGGGCGGCGGAC
GACCGGGATGACGGGAGGGG

Downstream 100 bases:

>100_bases
CGGCGATCAGGCCTCGAAGCGGCGGATGCTTTCGAGGTCGGCGCGGTCGAGCGGCTGCGTCAGCCACAACCCGCCGCGCC
CCTCACGCGACCAGCGCACA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MWAFALARYAPCPISGPMTRTITVAALQLALPGPVEPNIKAVTALVEAAAARGAQIILPPELFEGPYFCQVEEEELFATA
RPTAEHPSVVAMQALAAKCKVAIPTSFFERDGHHYYNTLAMIGPDGGIMGTYRKSHIPDGPGYEEKYYFRPGNTGFKIWE
VFDTRIGVGVCWDQWYPECARAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMQGHAVSNCMPVIAANRIGTEGDAR
FYGHSFIADEWGDLTQAFGASETGALVETIDLDRAAKHRAGMGFFRDRRPQLYGRLVEDV

Sequences:

>Translated_300_residues
MWAFALARYAPCPISGPMTRTITVAALQLALPGPVEPNIKAVTALVEAAAARGAQIILPPELFEGPYFCQVEEEELFATA
RPTAEHPSVVAMQALAAKCKVAIPTSFFERDGHHYYNTLAMIGPDGGIMGTYRKSHIPDGPGYEEKYYFRPGNTGFKIWE
VFDTRIGVGVCWDQWYPECARAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMQGHAVSNCMPVIAANRIGTEGDAR
FYGHSFIADEWGDLTQAFGASETGALVETIDLDRAAKHRAGMGFFRDRRPQLYGRLVEDV
>Mature_300_residues
MWAFALARYAPCPISGPMTRTITVAALQLALPGPVEPNIKAVTALVEAAAARGAQIILPPELFEGPYFCQVEEEELFATA
RPTAEHPSVVAMQALAAKCKVAIPTSFFERDGHHYYNTLAMIGPDGGIMGTYRKSHIPDGPGYEEKYYFRPGNTGFKIWE
VFDTRIGVGVCWDQWYPECARAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMQGHAVSNCMPVIAANRIGTEGDAR
FYGHSFIADEWGDLTQAFGASETGALVETIDLDRAAKHRAGMGFFRDRRPQLYGRLVEDV

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=285, Percent_Identity=29.4736842105263, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI7706509, Length=305, Percent_Identity=27.5409836065574, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI297632350, Length=296, Percent_Identity=28.3783783783784, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI5031947, Length=296, Percent_Identity=28.3783783783784, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI297632348, Length=296, Percent_Identity=28.3783783783784, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17533173, Length=312, Percent_Identity=27.8846153846154, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17556280, Length=281, Percent_Identity=25.6227758007117, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=294, Percent_Identity=24.4897959183673, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21358471, Length=305, Percent_Identity=28.8524590163934, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21355835, Length=287, Percent_Identity=26.1324041811847, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33084; Mature: 33084

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWAFALARYAPCPISGPMTRTITVAALQLALPGPVEPNIKAVTALVEAAAARGAQIILPP
CCHHHHHHCCCCCCCCCCHHHEEEEEHEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECH
ELFEGPYFCQVEEEELFATARPTAEHPSVVAMQALAAKCKVAIPTSFFERDGHHYYNTLA
HHHCCCEEEEECHHHHHEECCCCCCCCHHHHHHHHHHHCEEECCHHHHHCCCCEEEEEEE
MIGPDGGIMGTYRKSHIPDGPGYEEKYYFRPGNTGFKIWEVFDTRIGVGVCWDQWYPECA
EECCCCCEEECCHHCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCEEEHHHHHHHHH
RAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMQGHAVSNCMPVIAANRIGTEGDAR
HHHHHHCCHHEEEEHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCE
FYGHSFIADEWGDLTQAFGASETGALVETIDLDRAAKHRAGMGFFRDRRPQLYGRLVEDV
EECCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MWAFALARYAPCPISGPMTRTITVAALQLALPGPVEPNIKAVTALVEAAAARGAQIILPP
CCHHHHHHCCCCCCCCCCHHHEEEEEHEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECH
ELFEGPYFCQVEEEELFATARPTAEHPSVVAMQALAAKCKVAIPTSFFERDGHHYYNTLA
HHHCCCEEEEECHHHHHEECCCCCCCCHHHHHHHHHHHCEEECCHHHHHCCCCEEEEEEE
MIGPDGGIMGTYRKSHIPDGPGYEEKYYFRPGNTGFKIWEVFDTRIGVGVCWDQWYPECA
EECCCCCEEECCHHCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCEEEHHHHHHHHH
RAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMQGHAVSNCMPVIAANRIGTEGDAR
HHHHHHCCHHEEEEHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCE
FYGHSFIADEWGDLTQAFGASETGALVETIDLDRAAKHRAGMGFFRDRRPQLYGRLVEDV
EECCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]