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The map label for this gene is lipB

Identifier: 103486836

GI number: 103486836

Start: 1409413

End: 1410066

Strand: Direct

Name: lipB

Synonym: Sala_1350

Alternate gene names: 103486836

Gene position: 1409413-1410066 (Clockwise)

Preceding gene: 103486833

Following gene: 103486837

Centisome position: 42.13

GC content: 67.28

Gene sequence:

>654_bases
ATGCCTTCGCTTCCCGTGCCCCAATGGATCGTCAGCCCCGGTCTCACCGATTATGCCGCGGCGCTCGCCGACATGGAGTC
GCGCGCCGCCGCGATACATGCGGACGAAGCGGGCGAACGCATCTGGCTCCTCGAACACCCCCCGCTCTATACCGCGGGAA
CGAGCGCCGATCCCGCCGAACTGCTCGACCCGCGCTTTCCGGTTTACGATGCTGGCCGCGGCGGGCGTTATACCTATCAT
GGTCCGGGTCAGCGCGTCGGCTATGTCCAGCTCGACCTGACGCGGCGCGGGCGCGACGTGCGCGCCTATGTCCATGCGCT
CGAAGGCTGGGTGATCGATGCGCTCGCGCTGCTCGGCGTCAAGGCGCGGCGCGCCGAGGGACGCATCGGCATCTGGACCG
ACGACGTACGGGGACGCGAGGCGAAGATCGGCGCGATCGGGGTGCGCGTCAAACGCTGGGTGACGCTGCACGGCTTTTCG
CTGAACGTCGCGCCCGACCTGACCCATTTCACGGGTATCGTGCCATGCGGCATCGCCGAATATCCCGTCACGAGCCTCGC
CGCGCTAGGGAAGGCGACGGGTTTTTCCGAGGTCGATGCTGCGCTTGCCCGGACGCTCCCCGCTTTTCTCGACAAGCTGC
GGCCAAGCGATTAG

Upstream 100 bases:

>100_bases
CAACGAAAGTGACGATGTTCGCCGCGTCTGTCCAGCCCTGTCGCGCGATGCTCCGCAGGGCCTTGCACGGCGGGCCGCGA
CGCGCCAGATAGCCCGCGCC

Downstream 100 bases:

>100_bases
GACCGGCGCATGATCAGGATCCTTCTCGCTTCGCTCTCCCTGCTCACCCTCGCAGCGTGCGGCAGCAACGATAGCGGCGG
CAGTTCGACGACGAAGCTCG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MPSLPVPQWIVSPGLTDYAAALADMESRAAAIHADEAGERIWLLEHPPLYTAGTSADPAELLDPRFPVYDAGRGGRYTYH
GPGQRVGYVQLDLTRRGRDVRAYVHALEGWVIDALALLGVKARRAEGRIGIWTDDVRGREAKIGAIGVRVKRWVTLHGFS
LNVAPDLTHFTGIVPCGIAEYPVTSLAALGKATGFSEVDAALARTLPAFLDKLRPSD

Sequences:

>Translated_217_residues
MPSLPVPQWIVSPGLTDYAAALADMESRAAAIHADEAGERIWLLEHPPLYTAGTSADPAELLDPRFPVYDAGRGGRYTYH
GPGQRVGYVQLDLTRRGRDVRAYVHALEGWVIDALALLGVKARRAEGRIGIWTDDVRGREAKIGAIGVRVKRWVTLHGFS
LNVAPDLTHFTGIVPCGIAEYPVTSLAALGKATGFSEVDAALARTLPAFLDKLRPSD
>Mature_216_residues
PSLPVPQWIVSPGLTDYAAALADMESRAAAIHADEAGERIWLLEHPPLYTAGTSADPAELLDPRFPVYDAGRGGRYTYHG
PGQRVGYVQLDLTRRGRDVRAYVHALEGWVIDALALLGVKARRAEGRIGIWTDDVRGREAKIGAIGVRVKRWVTLHGFSL
NVAPDLTHFTGIVPCGIAEYPVTSLAALGKATGFSEVDAALARTLPAFLDKLRPSD

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=181, Percent_Identity=38.6740331491713, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87081767, Length=179, Percent_Identity=35.7541899441341, Blast_Score=113, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355483, Length=213, Percent_Identity=34.7417840375587, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_SPHAL (Q1GTF8)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616397.1
- ProteinModelPortal:   Q1GTF8
- SMR:   Q1GTF8
- GeneID:   4082818
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1350
- NMPDR:   fig|317655.9.peg.1283
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- ProtClustDB:   CLSK2777856
- BioCyc:   SALA317655:SALA_1350-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23477; Mature: 23346

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLPVPQWIVSPGLTDYAAALADMESRAAAIHADEAGERIWLLEHPPLYTAGTSADPAE
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCEECCCCCCHHH
LLDPRFPVYDAGRGGRYTYHGPGQRVGYVQLDLTRRGRDVRAYVHALEGWVIDALALLGV
HCCCCCCEEECCCCCCEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
KARRAEGRIGIWTDDVRGREAKIGAIGVRVKRWVTLHGFSLNVAPDLTHFTGIVPCGIAE
HHHCCCCCEEEEECCCCCCEEEEEEEEEEEEEEEEEECCEEECCCCHHHHHCCCCCCCCC
YPVTSLAALGKATGFSEVDAALARTLPAFLDKLRPSD
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSLPVPQWIVSPGLTDYAAALADMESRAAAIHADEAGERIWLLEHPPLYTAGTSADPAE
CCCCCCHHHHCCCHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCEECCCCCCHHH
LLDPRFPVYDAGRGGRYTYHGPGQRVGYVQLDLTRRGRDVRAYVHALEGWVIDALALLGV
HCCCCCCEEECCCCCCEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
KARRAEGRIGIWTDDVRGREAKIGAIGVRVKRWVTLHGFSLNVAPDLTHFTGIVPCGIAE
HHHCCCCCEEEEECCCCCCEEEEEEEEEEEEEEEEEECCEEECCCCHHHHHCCCCCCCCC
YPVTSLAALGKATGFSEVDAALARTLPAFLDKLRPSD
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA