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The map label for this gene is thiE [H]

Identifier: 103486801

GI number: 103486801

Start: 1373496

End: 1374146

Strand: Reverse

Name: thiE [H]

Synonym: Sala_1315

Alternate gene names: 103486801

Gene position: 1374146-1373496 (Counterclockwise)

Preceding gene: 103486803

Following gene: 103486800

Centisome position: 41.08

GC content: 66.36

Gene sequence:

>651_bases
ATGACCACCGCACCGACTGCACCGACCTGCCAGCTTTACTTGATCTCGCCGCTCGATGTCGGTGGCGATTTTCCTGCGCG
GCTGGAGGAGGCGCTGGCCGCGGGGCCAGTCGCGGCGTTCCAGTTTCGCGTCAAGGGGCTGGACCAGCATGACGCGGCGC
GCCTCGCGGAGCCTTTGCGGGCGATTTGCGCGGAGCATGACGTGGCCTTCATCGTCAATGACGATGTCGCGCTGGCAAAG
CGGCTGAAGGCCGATGGCGTGCATCTGGGGCAGGGCGATGGCGATCCGAAGGAGGCGCGCCGCCAACTCGGTCCCGATGC
TCAGATCGGCGTGACGTGCCATGATAGCCGCCATCTGGCGATGGATGCGGGCGAGGCGGGCGCCGACTATGTGGCCTTCG
GTGCCTTTTTCCCGACGACGACCAAGACGGTCGAGCATCGCGCCGACCCCGAAATCCTGACCTGGTGGCAGGGCCTGTTC
GAGCTGCCCTGCGTGGCGATCGGGGGAATCACGATCGACAACGCGAAAACACTCGCCGATGCCGGGGCCGACTTTGTCGC
GGTATCGGGCGGCGTCTGGAATTATCGTGACGGACCCGGCGCCGCGGTGCGCGCCTTTGCCGAAATCCTGGGCAATCAGC
CCGCGGGATAG

Upstream 100 bases:

>100_bases
GCACAGGCGGAAAGGAGGAGAGCGGAAGGAAAGGCGACGGTCGCAAGGTGCATGATCTTCTGCATTTGCCCGCATCTGGC
GGCGTCGCTATGAACGCGCC

Downstream 100 bases:

>100_bases
TCACGTTCCGGCTTTTTCGGGTCGCCCTTGTCTTTCGGCGCCGCCGCCGCATCCTTGCGCCATCCGAACAGGCCGACGCA
GGGCGCTCCGCGCGGCGAAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTTAPTAPTCQLYLISPLDVGGDFPARLEEALAAGPVAAFQFRVKGLDQHDAARLAEPLRAICAEHDVAFIVNDDVALAK
RLKADGVHLGQGDGDPKEARRQLGPDAQIGVTCHDSRHLAMDAGEAGADYVAFGAFFPTTTKTVEHRADPEILTWWQGLF
ELPCVAIGGITIDNAKTLADAGADFVAVSGGVWNYRDGPGAAVRAFAEILGNQPAG

Sequences:

>Translated_216_residues
MTTAPTAPTCQLYLISPLDVGGDFPARLEEALAAGPVAAFQFRVKGLDQHDAARLAEPLRAICAEHDVAFIVNDDVALAK
RLKADGVHLGQGDGDPKEARRQLGPDAQIGVTCHDSRHLAMDAGEAGADYVAFGAFFPTTTKTVEHRADPEILTWWQGLF
ELPCVAIGGITIDNAKTLADAGADFVAVSGGVWNYRDGPGAAVRAFAEILGNQPAG
>Mature_215_residues
TTAPTAPTCQLYLISPLDVGGDFPARLEEALAAGPVAAFQFRVKGLDQHDAARLAEPLRAICAEHDVAFIVNDDVALAKR
LKADGVHLGQGDGDPKEARRQLGPDAQIGVTCHDSRHLAMDAGEAGADYVAFGAFFPTTTKTVEHRADPEILTWWQGLFE
LPCVAIGGITIDNAKTLADAGADFVAVSGGVWNYRDGPGAAVRAFAEILGNQPAG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=137, Percent_Identity=31.3868613138686, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22704; Mature: 22572

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAPTAPTCQLYLISPLDVGGDFPARLEEALAAGPVAAFQFRVKGLDQHDAARLAEPLR
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHEEEEEECCCCCHHHHHHHHHHH
AICAEHDVAFIVNDDVALAKRLKADGVHLGQGDGDPKEARRQLGPDAQIGVTCHDSRHLA
HHHCCCCEEEEEECCHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCEEEEEEECCCEEE
MDAGEAGADYVAFGAFFPTTTKTVEHRADPEILTWWQGLFELPCVAIGGITIDNAKTLAD
EECCCCCCCEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCEEECCEEECCCHHHHH
AGADFVAVSGGVWNYRDGPGAAVRAFAEILGNQPAG
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTAPTAPTCQLYLISPLDVGGDFPARLEEALAAGPVAAFQFRVKGLDQHDAARLAEPLR
CCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHEEEEEECCCCCHHHHHHHHHHH
AICAEHDVAFIVNDDVALAKRLKADGVHLGQGDGDPKEARRQLGPDAQIGVTCHDSRHLA
HHHCCCCEEEEEECCHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCCEEEEEEECCCEEE
MDAGEAGADYVAFGAFFPTTTKTVEHRADPEILTWWQGLFELPCVAIGGITIDNAKTLAD
EECCCCCCCEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCEEECCEEECCCHHHHH
AGADFVAVSGGVWNYRDGPGAAVRAFAEILGNQPAG
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA