Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 103486796

GI number: 103486796

Start: 1369943

End: 1370476

Strand: Reverse

Name: nuoB

Synonym: Sala_1310

Alternate gene names: 103486796

Gene position: 1370476-1369943 (Counterclockwise)

Preceding gene: 103486797

Following gene: 103486795

Centisome position: 40.97

GC content: 64.04

Gene sequence:

>534_bases
ATGCCCGGCCAGATGCCGGTCGCGCCGGGGACCAACCCCGACCAGTCGTTTTTCGACGATTTGAGCGGCGAGGTCGGCGA
CAAGGGCTTTCTCGTCACCTCGACCGAGGATCTGTTCAACTGGGCGCGCACCGGCTCGCTGTGGTGGATGACCTTTGGCC
TTGCGTGCTGCGCGGTCGAGATGATCCACGTCAACATGCCGCGTTACGACATGGAGCGTTTTGGCGTCGCGCCGCGCGCG
TCCCCACGCCAGTCGGACGTGATGATCGTCGCGGGGACGCTGTGCAACAAGATGGCCCCCGCGCTCCGCCGCGTGTACGA
CCAGATGTCGAACCCCAAATATGTCATCTCGATGGGCAGCTGCGCCAATGGCGGCGGCTATTATCACTACAGCTATTCGG
TGGTGCGCGGCTGCGATCGCATCGTGCCGGTCGACATCTATGTCCCCGGCTGCCCGCCGACCGCCGAAGCCTTGCTCTAT
GGCGTGATGCAATTGCAGCGGAAGATCCGCCGCACCGGGACGATCGAACGCTGA

Upstream 100 bases:

>100_bases
GGGTCACGATGATGGTTTTCCTTGCCGAGCTTGCGATCGGCCTGATTTACGCATGGAAGAAAGGGGCGTTGGATTGGGAA
TGAGCAACAGCACCATCCTG

Downstream 100 bases:

>100_bases
TGGCCAGTCCCGCTCCCCTTGTCGCCCCGCGCGCGGGCCTTGCCGCCGCGCTCGAAAAACTGCTCGGCGACGATTTGCTC
ACGCATGTCTTCACCGTCGG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MPGQMPVAPGTNPDQSFFDDLSGEVGDKGFLVTSTEDLFNWARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGVAPRA
SPRQSDVMIVAGTLCNKMAPALRRVYDQMSNPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLY
GVMQLQRKIRRTGTIER

Sequences:

>Translated_177_residues
MPGQMPVAPGTNPDQSFFDDLSGEVGDKGFLVTSTEDLFNWARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGVAPRA
SPRQSDVMIVAGTLCNKMAPALRRVYDQMSNPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLY
GVMQLQRKIRRTGTIER
>Mature_176_residues
PGQMPVAPGTNPDQSFFDDLSGEVGDKGFLVTSTEDLFNWARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGVAPRAS
PRQSDVMIVAGTLCNKMAPALRRVYDQMSNPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYG
VMQLQRKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.2312925170068, Blast_Score=251, Evalue=2e-67,
Organism=Escherichia coli, GI1788624, Length=152, Percent_Identity=51.3157894736842, Blast_Score=157, Evalue=3e-40,
Organism=Escherichia coli, GI1789074, Length=124, Percent_Identity=40.3225806451613, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=38.0165289256198, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=71.1267605633803, Blast_Score=228, Evalue=9e-61,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=76.5957446808511, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=76.5957446808511, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=75.886524822695, Blast_Score=239, Evalue=7e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_SPHAL (Q1GTJ8)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616357.1
- ProteinModelPortal:   Q1GTJ8
- SMR:   Q1GTJ8
- GeneID:   4082234
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1310
- NMPDR:   fig|317655.9.peg.1244
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- ProtClustDB:   PRK06411
- BioCyc:   SALA317655:SALA_1310-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19650; Mature: 19519

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
9.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGQMPVAPGTNPDQSFFDDLSGEVGDKGFLVTSTEDLFNWARTGSLWWMTFGLACCAVE
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHH
MIHVNMPRYDMERFGVAPRASPRQSDVMIVAGTLCNKMAPALRRVYDQMSNPKYVISMGS
HHHCCCCCHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
CANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGVMQLQRKIRRTGTIER
CCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PGQMPVAPGTNPDQSFFDDLSGEVGDKGFLVTSTEDLFNWARTGSLWWMTFGLACCAVE
CCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHH
MIHVNMPRYDMERFGVAPRASPRQSDVMIVAGTLCNKMAPALRRVYDQMSNPKYVISMGS
HHHCCCCCHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
CANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGVMQLQRKIRRTGTIER
CCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA