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The map label for this gene is nasA [H]

Identifier: 103486754

GI number: 103486754

Start: 1322376

End: 1325030

Strand: Direct

Name: nasA [H]

Synonym: Sala_1268

Alternate gene names: 103486754

Gene position: 1322376-1325030 (Clockwise)

Preceding gene: 103486753

Following gene: 103486755

Centisome position: 39.53

GC content: 68.06

Gene sequence:

>2655_bases
GTGCCCCGCACGGTTGAGGATCGTCCGGGGGACGATCCGACCCGGCGCCCCCCTGAAGGGGAGAGGGCTATAAGGACCAC
CTGCGCCTATTGCGGCGTCGGCTGCGGCATAAGCGCAACGGTGACCGGCGAACGCACGGTCGATATCGCAGGCGACCCCG
ACCATCCCGCCAACCGCGGCAAACTCTGTTCCAAGGGCACGCACCTCGGCGAAACGGTCGGGCTCGAAGGCCGCCTTCTC
CATCCGATGATCGGCAACAAGCGCGCCAGCTGGGACAAGGCGCTCGACCTCGTCGCCAAGCGCTTCAAGGAGGTGATCGC
GCGCCATGGTCCGAACAGCGTCGCCTTCTATGTGTCGGGCCAGCTCCTGACCGAGGATTATTATGTCGCGAACAAGCTGA
TGAAGGGCTTCATCGGCACCGCCAACATCGACACCAACTCAAGGCTGTGCATGTCGAGCGCGGTCGCCGGGCACATGCGC
GCGTTCGGCGAGGACGTCGTTCCCGCGACCTATGCCGACCTCGACGCGGCCGACCTGTTCATCCTTGTCGGATCGAACAC
CGCCTGGTGCCATCCGGTCGTTTATCAGCGCATCCGCGCCCGCTGCGAGGCGGGAGCCAAGCTCGTCGTCATCGACCCGC
GCCGCACCGAAACCGCTGAGGAAGCCGATCTTCACCTCGCGATTCGCCCCGGCAGCGACGTGGCGCTGATGAACGGCCTG
CTCCAGTACAGCCGCGAGGCGGGCGCGATCGACGAGGCTTATCTTGCCGCGCATGTCGCGGTGCCAGAGGATTTCTGGGA
CAGGCTTGGAGAGGGGAATGACCTGTGGTCGGTGGCGCGCATCTGCGATGTTGCCGCCGCCGACCTCAGGCATTTCTACG
ACCTCTTCGCAGCGACCCCGCGCGCCGTCACCCTGTTCAGCCAGGGGATAAACCAGTCGACCGCGGGCACCGACCAGGTG
AATGCGATCCTCAACCTCCACCTCGCAACCGGGCGCATCGGCAAGCCGGGCGCCGCCCCCTTTTCGATCACCGGCCAGCC
CAATGCGATGGGCGGGCGCGAAGTCGGCGGTCTCGCGTCGACGCTCGCCGCGCACATGGATTTCGGACAGGAAAACCGCG
CGCGCGTCCAGCGCTTCTGGGCGGCGCCGACAATGGCCGAAAAGCCGGGCCTGAAAGCCGTCGACATGTTCCGCGCGATC
GGCGAGGGGCGGATCAGGGCGCTGTGGGTGATGGCGACCAATCCGGCGGTGTCGATGCCCGACGCGGGCCGCGTGCGCGA
GGCGCTCGCCGCCTGCCCCTTTGTCGTCGTCAGCGACGTCATGGAAAAGACCGATACCGGCGCCTTCGCGCACGTCCGCC
TGCCCGCCGCCGCCTGGGGCGAAAAGGACGGCACCGTCACGGGCAGCGACCGCACGATCAGCCGCCAGCGCGCACTTTTC
GCGCTTCCCGGCGAAGCGATGCCCGACTGGTGGATCGTCAAGGAAGTCGCGCGGCGCATGGGCTGGAAAAACGCCTTCAC
CTACGACCGCCCCGCCGACATCTGGCGCGAGCATTGCCGTCTGTCGAATTATGACAATGACGGTGCGCGCATCTTTTCCC
TGCCGGGTGCGGCACTGGGCGGCAATGGAGCCTATGATGCCATGGAGCCTTTTCGCTGGGGCGGCGATCATCCGTTCGCC
GAGGGGCGGTTCCCGACCGAAGACGGCCGGGCGCGCCTCGTCCCCGTTGCGCAAAGGCCGCTGCCGGAACCGCTCGCCAA
ATGGCCCCTCACACTGAACACCGGCCGCTACCGCGACCAGTGGCATACGATGACGCGCACCGGACTCGCTCCAAAGCTGG
CACGTCACCGCGAGGAGCCGCTCGTCGAGGTCCACCCCGACGACGGTACGCGACTCGGCCTTACCGACGGTGGCCTTGCG
CACGTGGAAACGCCGCAGGGCGACAGCCTCTACCGCGTCGCGTTCCACGATGGCCAGCGCCCCGGCGAGATCTTTGTTCC
GATCCACTGGACCGACCGCACCAGCAGCGGCGGCCGCACCGGCCTGCTCCCGCGCCCGCTCGTCGACCCTCTCTCGGGCC
AGCCGGGTTTCAAGCGCACCCCCGCCGCCATCGCTCCGGTCCAGACGAGATGGCGCGCCTTCCTGCTGCTCGCACGCGAA
CTGGCAACCACGCCGCGCTGCCTGTGGGCGACGCGCGTCGCGGTGCCAGCGGGCGTGCTGTGGGAACTGGCAGGCAATGG
CGATCTCAAGGCAATCGAGGCGCTTCTGCCCAAGGGCGAACATATTGAAGCGCACGACGCTGCACGCGGGACGCGGCGTA
TCGCCATCGTCGCGAACGGCCGTCTCGCGGGCGCGCTGTTCGCGACCGAAACCGGCAATCTGCCCCCCCGCGACTGGTTG
ATCGCACAACTTGCCGCGCCCGAAATCGCCCCCACGCTGCTCGCCGGTCGCGCGCCGGGCGTACAGGCCGACCGCGGGCC
GATCGTCTGCGTCTGTTTCGACATCGGGCTCAGAACGATCGTCGCCGCGATCCGCGACCAGAACCTCGCCGATGTTGCGG
CGATCGGCGCTGCGCTGGGCGCGGGCACCAACTGCGGGTCGTGCCGCCCCGCGCTTGCCCGTATTCTGACCGAGGAGACC
AGCAATGCAGCCTGA

Upstream 100 bases:

>100_bases
ATCACCCTCTCCCCTTCAGGGGAGAGGGCCGGGGAGAGGGGAGCGACAGTTGAGCGCTTCCCTGCCCCCGCCCCCTCTCC
CAACCGGTGCCGGGTCAACC

Downstream 100 bases:

>100_bases
TGATTTCGCCCCCGGCACGGTTTGGCTCGTCGGTGCAGGCCCCGGCGATCCCGACCTGCTGACGATGAAGGCCGTGCGAT
TGATCAGCCGCGCCGATATC

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 884; Mature: 883

Protein sequence:

>884_residues
MPRTVEDRPGDDPTRRPPEGERAIRTTCAYCGVGCGISATVTGERTVDIAGDPDHPANRGKLCSKGTHLGETVGLEGRLL
HPMIGNKRASWDKALDLVAKRFKEVIARHGPNSVAFYVSGQLLTEDYYVANKLMKGFIGTANIDTNSRLCMSSAVAGHMR
AFGEDVVPATYADLDAADLFILVGSNTAWCHPVVYQRIRARCEAGAKLVVIDPRRTETAEEADLHLAIRPGSDVALMNGL
LQYSREAGAIDEAYLAAHVAVPEDFWDRLGEGNDLWSVARICDVAAADLRHFYDLFAATPRAVTLFSQGINQSTAGTDQV
NAILNLHLATGRIGKPGAAPFSITGQPNAMGGREVGGLASTLAAHMDFGQENRARVQRFWAAPTMAEKPGLKAVDMFRAI
GEGRIRALWVMATNPAVSMPDAGRVREALAACPFVVVSDVMEKTDTGAFAHVRLPAAAWGEKDGTVTGSDRTISRQRALF
ALPGEAMPDWWIVKEVARRMGWKNAFTYDRPADIWREHCRLSNYDNDGARIFSLPGAALGGNGAYDAMEPFRWGGDHPFA
EGRFPTEDGRARLVPVAQRPLPEPLAKWPLTLNTGRYRDQWHTMTRTGLAPKLARHREEPLVEVHPDDGTRLGLTDGGLA
HVETPQGDSLYRVAFHDGQRPGEIFVPIHWTDRTSSGGRTGLLPRPLVDPLSGQPGFKRTPAAIAPVQTRWRAFLLLARE
LATTPRCLWATRVAVPAGVLWELAGNGDLKAIEALLPKGEHIEAHDAARGTRRIAIVANGRLAGALFATETGNLPPRDWL
IAQLAAPEIAPTLLAGRAPGVQADRGPIVCVCFDIGLRTIVAAIRDQNLADVAAIGAALGAGTNCGSCRPALARILTEET
SNAA

Sequences:

>Translated_884_residues
MPRTVEDRPGDDPTRRPPEGERAIRTTCAYCGVGCGISATVTGERTVDIAGDPDHPANRGKLCSKGTHLGETVGLEGRLL
HPMIGNKRASWDKALDLVAKRFKEVIARHGPNSVAFYVSGQLLTEDYYVANKLMKGFIGTANIDTNSRLCMSSAVAGHMR
AFGEDVVPATYADLDAADLFILVGSNTAWCHPVVYQRIRARCEAGAKLVVIDPRRTETAEEADLHLAIRPGSDVALMNGL
LQYSREAGAIDEAYLAAHVAVPEDFWDRLGEGNDLWSVARICDVAAADLRHFYDLFAATPRAVTLFSQGINQSTAGTDQV
NAILNLHLATGRIGKPGAAPFSITGQPNAMGGREVGGLASTLAAHMDFGQENRARVQRFWAAPTMAEKPGLKAVDMFRAI
GEGRIRALWVMATNPAVSMPDAGRVREALAACPFVVVSDVMEKTDTGAFAHVRLPAAAWGEKDGTVTGSDRTISRQRALF
ALPGEAMPDWWIVKEVARRMGWKNAFTYDRPADIWREHCRLSNYDNDGARIFSLPGAALGGNGAYDAMEPFRWGGDHPFA
EGRFPTEDGRARLVPVAQRPLPEPLAKWPLTLNTGRYRDQWHTMTRTGLAPKLARHREEPLVEVHPDDGTRLGLTDGGLA
HVETPQGDSLYRVAFHDGQRPGEIFVPIHWTDRTSSGGRTGLLPRPLVDPLSGQPGFKRTPAAIAPVQTRWRAFLLLARE
LATTPRCLWATRVAVPAGVLWELAGNGDLKAIEALLPKGEHIEAHDAARGTRRIAIVANGRLAGALFATETGNLPPRDWL
IAQLAAPEIAPTLLAGRAPGVQADRGPIVCVCFDIGLRTIVAAIRDQNLADVAAIGAALGAGTNCGSCRPALARILTEET
SNAA
>Mature_883_residues
PRTVEDRPGDDPTRRPPEGERAIRTTCAYCGVGCGISATVTGERTVDIAGDPDHPANRGKLCSKGTHLGETVGLEGRLLH
PMIGNKRASWDKALDLVAKRFKEVIARHGPNSVAFYVSGQLLTEDYYVANKLMKGFIGTANIDTNSRLCMSSAVAGHMRA
FGEDVVPATYADLDAADLFILVGSNTAWCHPVVYQRIRARCEAGAKLVVIDPRRTETAEEADLHLAIRPGSDVALMNGLL
QYSREAGAIDEAYLAAHVAVPEDFWDRLGEGNDLWSVARICDVAAADLRHFYDLFAATPRAVTLFSQGINQSTAGTDQVN
AILNLHLATGRIGKPGAAPFSITGQPNAMGGREVGGLASTLAAHMDFGQENRARVQRFWAAPTMAEKPGLKAVDMFRAIG
EGRIRALWVMATNPAVSMPDAGRVREALAACPFVVVSDVMEKTDTGAFAHVRLPAAAWGEKDGTVTGSDRTISRQRALFA
LPGEAMPDWWIVKEVARRMGWKNAFTYDRPADIWREHCRLSNYDNDGARIFSLPGAALGGNGAYDAMEPFRWGGDHPFAE
GRFPTEDGRARLVPVAQRPLPEPLAKWPLTLNTGRYRDQWHTMTRTGLAPKLARHREEPLVEVHPDDGTRLGLTDGGLAH
VETPQGDSLYRVAFHDGQRPGEIFVPIHWTDRTSSGGRTGLLPRPLVDPLSGQPGFKRTPAAIAPVQTRWRAFLLLAREL
ATTPRCLWATRVAVPAGVLWELAGNGDLKAIEALLPKGEHIEAHDAARGTRRIAIVANGRLAGALFATETGNLPPRDWLI
AQLAAPEIAPTLLAGRAPGVQADRGPIVCVCFDIGLRTIVAAIRDQNLADVAAIGAALGAGTNCGSCRPALARILTEETS
NAA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=702, Percent_Identity=27.6353276353276, Blast_Score=269, Evalue=5e-73,
Organism=Escherichia coli, GI1788534, Length=529, Percent_Identity=31.758034026465, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI3868720, Length=619, Percent_Identity=25.0403877221325, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI3868719, Length=608, Percent_Identity=24.3421052631579, Blast_Score=158, Evalue=2e-39,
Organism=Escherichia coli, GI1787778, Length=391, Percent_Identity=28.1329923273657, Blast_Score=114, Evalue=4e-26,
Organism=Escherichia coli, GI1787870, Length=717, Percent_Identity=22.4546722454672, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87081797, Length=556, Percent_Identity=23.021582733813, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI171474008, Length=561, Percent_Identity=21.9251336898396, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI145693196, Length=668, Percent_Identity=22.4550898203593, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 95326; Mature: 95195

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTVEDRPGDDPTRRPPEGERAIRTTCAYCGVGCGISATVTGERTVDIAGDPDHPANRG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCCCCCC
KLCSKGTHLGETVGLEGRLLHPMIGNKRASWDKALDLVAKRFKEVIARHGPNSVAFYVSG
CCCCCCCCCCHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
QLLTEDYYVANKLMKGFIGTANIDTNSRLCMSSAVAGHMRAFGEDVVPATYADLDAADLF
EEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCEEE
ILVGSNTAWCHPVVYQRIRARCEAGAKLVVIDPRRTETAEEADLHLAIRPGSDVALMNGL
EEECCCCCEECHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHH
LQYSREAGAIDEAYLAAHVAVPEDFWDRLGEGNDLWSVARICDVAAADLRHFYDLFAATP
HHHHHHCCCCHHHHHHEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RAVTLFSQGINQSTAGTDQVNAILNLHLATGRIGKPGAAPFSITGQPNAMGGREVGGLAS
HHHHHHHHCCCCCCCCHHHEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHH
TLAAHMDFGQENRARVQRFWAAPTMAEKPGLKAVDMFRAIGEGRIRALWVMATNPAVSMP
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCC
DAGRVREALAACPFVVVSDVMEKTDTGAFAHVRLPAAAWGEKDGTVTGSDRTISRQRALF
CCHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCEECCCCCCHHHCCEEE
ALPGEAMPDWWIVKEVARRMGWKNAFTYDRPADIWREHCRLSNYDNDGARIFSLPGAALG
ECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCC
GNGAYDAMEPFRWGGDHPFAEGRFPTEDGRARLVPVAQRPLPEPLAKWPLTLNTGRYRDQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCEEECCCCCHHH
WHTMTRTGLAPKLARHREEPLVEVHPDDGTRLGLTDGGLAHVETPQGDSLYRVAFHDGQR
HHHHHHHCCCHHHHHHCCCCEEEECCCCCCEECCCCCCEEEEECCCCCCEEEEEEECCCC
PGEIFVPIHWTDRTSSGGRTGLLPRPLVDPLSGQPGFKRTPAAIAPVQTRWRAFLLLARE
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
LATTPRCLWATRVAVPAGVLWELAGNGDLKAIEALLPKGEHIEAHDAARGTRRIAIVANG
HHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCHHHHCCCCEEEEEEECC
RLAGALFATETGNLPPRDWLIAQLAAPEIAPTLLAGRAPGVQADRGPIVCVCFDIGLRTI
CEEEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHH
VAAIRDQNLADVAAIGAALGAGTNCGSCRPALARILTEETSNAA
HHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PRTVEDRPGDDPTRRPPEGERAIRTTCAYCGVGCGISATVTGERTVDIAGDPDHPANRG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCCCCCCCC
KLCSKGTHLGETVGLEGRLLHPMIGNKRASWDKALDLVAKRFKEVIARHGPNSVAFYVSG
CCCCCCCCCCHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
QLLTEDYYVANKLMKGFIGTANIDTNSRLCMSSAVAGHMRAFGEDVVPATYADLDAADLF
EEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCEEE
ILVGSNTAWCHPVVYQRIRARCEAGAKLVVIDPRRTETAEEADLHLAIRPGSDVALMNGL
EEECCCCCEECHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHH
LQYSREAGAIDEAYLAAHVAVPEDFWDRLGEGNDLWSVARICDVAAADLRHFYDLFAATP
HHHHHHCCCCHHHHHHEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RAVTLFSQGINQSTAGTDQVNAILNLHLATGRIGKPGAAPFSITGQPNAMGGREVGGLAS
HHHHHHHHCCCCCCCCHHHEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHH
TLAAHMDFGQENRARVQRFWAAPTMAEKPGLKAVDMFRAIGEGRIRALWVMATNPAVSMP
HHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCC
DAGRVREALAACPFVVVSDVMEKTDTGAFAHVRLPAAAWGEKDGTVTGSDRTISRQRALF
CCHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCEECCCCCCHHHCCEEE
ALPGEAMPDWWIVKEVARRMGWKNAFTYDRPADIWREHCRLSNYDNDGARIFSLPGAALG
ECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCC
GNGAYDAMEPFRWGGDHPFAEGRFPTEDGRARLVPVAQRPLPEPLAKWPLTLNTGRYRDQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHCCEEECCCCCHHH
WHTMTRTGLAPKLARHREEPLVEVHPDDGTRLGLTDGGLAHVETPQGDSLYRVAFHDGQR
HHHHHHHCCCHHHHHHCCCCEEEECCCCCCEECCCCCCEEEEECCCCCCEEEEEEECCCC
PGEIFVPIHWTDRTSSGGRTGLLPRPLVDPLSGQPGFKRTPAAIAPVQTRWRAFLLLARE
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
LATTPRCLWATRVAVPAGVLWELAGNGDLKAIEALLPKGEHIEAHDAARGTRRIAIVANG
HHCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCHHHHCCCCEEEEEEECC
RLAGALFATETGNLPPRDWLIAQLAAPEIAPTLLAGRAPGVQADRGPIVCVCFDIGLRTI
CEEEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHH
VAAIRDQNLADVAAIGAALGAGTNCGSCRPALARILTEETSNAA
HHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]