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The map label for this gene is nasD [H]

Identifier: 103486752

GI number: 103486752

Start: 1319457

End: 1321934

Strand: Direct

Name: nasD [H]

Synonym: Sala_1266

Alternate gene names: 103486752

Gene position: 1319457-1321934 (Clockwise)

Preceding gene: 103486751

Following gene: 103486753

Centisome position: 39.44

GC content: 65.94

Gene sequence:

>2478_bases
ATGGAACATCGCCCCCTCTCCCCCGACGCCGACACGCGCGCGCATCTCGTCGTCATCGGCAACGGCATGGCCGGATGCCG
CGCGGTCGAGGAGCTGCTCGCGCGCGACCCGGCGCGCTATCGCGTGACGATCTTCGGCGCAGAGCCGCGCGTCAATTACA
ATCGCATCATGCTTTCGCCCGTGCTGGCAGGCGAGAAAAGCTTCGAGGATATCGTCATCAACGATGCCGACTGGTATGCG
GACAATGGCATCGCGCTGGTCGCGGGCGACCCGGTGGTTGCACTCGATCGCGCGGCCAGGACCGTGACGACGCGCGGCGG
CATCGTCGAAACCTATGACAAGCTGGTGATTGCGACGGGGTCCGATCCTTTCATCATTCCCGTTCCCGGCAAGGATTTGC
CCGGCGTCATTGCCTTCCGCGACATGGACGATGTCGGCACGATGCTTAGCGCCGCGGAACAGGGCGGCAGCGCCGTCGTC
ATCGGCGGCGGGCTGCTCGGGCTCGAGGCAGCGCATGGCCTGTCCTTGCGCGGCATGAAGGTCACCGTGCTCCACCTCAT
GCCGACCCTGATGGAACGCCAGCTCGACGAAGCGGCGGGCTGGCTCCTGAAACAGGCGCTGGAAGCGCGCGGCCAGACGA
TCCTGACCGGCGCCGACACCGCCGAGATCGTCGGCGAGGTCAAGGTCGAGGGCGTAAAGCTCAAGGATGGCACGCTGATC
CCCGCCGATCTGGTGGTCATGGCGGTCGGCATCCGCCCCTCGATCGCGCTCGCACGCGATGCCGGGCTTGCCGTCGGGCG
GGGCATCTTGGTCGATGACCATATGGTGACCAGCGATCCCGACATTCTCGCGGTCGGCGAATGCGTAGAGCATGACGGAC
AGGTTTACGGCCTCGTCGCGCCGCTCTGGGACATGTGCCGCAGCCTCGCCGACGGTCTGGTCGAACGACCGTCGGGCTAT
CGGGGTTCGGTGACCTCGACCAAGCTGAAGGTGTCGGGGATCGACGTCTTTTCGGCGGGCGATTTTTCAGGCGGCGACGG
TTGCGAGGACATCGTGCTGCGCGATGCAAGCCGCGGCGTCTACAAGCGCGTGATCGTCAAGGACGACCGCATCGTTGGCG
CGGTGCTCTATGGCGATACGGCCGACGGCAGCTGGTACTTCGACCTGCTCAAACGCCAGGAGGATGTCTCCGGCCTCCGC
GATCTGTTGATCTTCGGCCAGGCCTTCGCCGCTGGAGGAGGCGCGGCGGACCCTAAGGCGGCCGTTGCGGCGCTCTCGGA
CGATGCCGAGATTTGCGGCTGCAACGGCGTCACCAAGGGCCAGGTCGTGTCGTGCATCACCAAGGGAGCGCACAGCCTCG
ACGCGGTGCGCAGCACCTGCAAGGCGTCGGCGAGCTGTGGCAGCTGCACCGGCCTCGTCGAGACCCTGCTCGCGCTGACG
CTCGGCGACGAGGTGCAGGCCGGGCCCAAGACGATGTGCAAATGCACCAGCTTCGGTCACGACGACGTCCGCCGCGAGAT
CGTCGCGCAGAATATGCGCTCGATCCCCGAAGTGATGCAAAAGCTGCACTGGACGACCCCCGACGGCTGCTCCTCCTGTC
GTCCGGCGCTCAACTATTATCTGCTCTGCGCGCTGCCCGGCGACTATGTCGACGACCAGCAGAGCCGCTTCGTCAACGAG
CGCCTCCACGCCAATATCCAGAAGGACGGGACCTACTCGGTCGTGCCGCGCATGTGGGGCGGGCTGACTAACCCGACCGA
ACTGCGCGCGATCGCCGATGTCGTCGAGAAGTACAATGCGCCGATGGTCAAGGTGACCGGCGGCCAGCGGCTCGACATCT
TCGGCATCAAGAAGGAGGATCTGCCCGCGGTCTGGGCCGACCTCAATGCCGCCGGGATGGTCTCGGGCCACGCCTATGGC
AAGGCGCTGCGCACGGTAAAAACCTGCGTCGGGTCCGAATGGTGCCGCTTCGGCACGCAGGATTCGACCGGCCTCGGCGT
CAAGATCGAGCGGATGAGCTGGGGCAGCTGGATGCCGCACAAGTTCAAGATCGCCGTGTCGGGCTGCCCGCGCAACTGCG
CCGAGGCGACGATCAAGGATTTCGGCGTCGTGTGCGTCGACAGCGGCTATGAGCTGCACATCGGCGGCAATGGCGGCATC
CATGTCCGCGCGACCGACCTGCTCTGCAAGGTCGCGACCGAACAGGAGGCGATGGACTATTGCGCTGCCTTCATCCAGCT
CTACCGCGAGGAAGCGCGCTATCTTGAGCGCACCGCGCCGTGGATTGAGCGCGTCGGGCTCGACTATGTGAAGGGGCGGT
TGCTCGACGATCCGGCGGGCCGCGAGGCGCTGCGCGCTCGCTTTCTCCATTCGCAAAGCTTCAGTCAGGACGATCCCTGG
GCACAGCGCGCCGAGGGCGCCGAGGCCGAACATCATGCGCCGATGGCGCGCTTCATCCCGCAGGAGGAACTGGCATGA

Upstream 100 bases:

>100_bases
TGGGCAAGATCACCGGTTCGTTACGTTACGCGCATTATCGAGCCAACAGCTTCGCCACCGGTACCGATAAAATCTGGCTG
CAATTCGATTGGACGCTTTG

Downstream 100 bases:

>100_bases
CCACCACCGCAGACTGGCTCGACATCGGCTGGGTCGACCAGATTCCGGTGCGCGGCAGTCGCACCGTGCAGGTCGCGGGC
GGCGACGATATCGCGGTGTT

Product: nitrite reductase (NAD(P)H) large subunit, NirB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 825; Mature: 825

Protein sequence:

>825_residues
MEHRPLSPDADTRAHLVVIGNGMAGCRAVEELLARDPARYRVTIFGAEPRVNYNRIMLSPVLAGEKSFEDIVINDADWYA
DNGIALVAGDPVVALDRAARTVTTRGGIVETYDKLVIATGSDPFIIPVPGKDLPGVIAFRDMDDVGTMLSAAEQGGSAVV
IGGGLLGLEAAHGLSLRGMKVTVLHLMPTLMERQLDEAAGWLLKQALEARGQTILTGADTAEIVGEVKVEGVKLKDGTLI
PADLVVMAVGIRPSIALARDAGLAVGRGILVDDHMVTSDPDILAVGECVEHDGQVYGLVAPLWDMCRSLADGLVERPSGY
RGSVTSTKLKVSGIDVFSAGDFSGGDGCEDIVLRDASRGVYKRVIVKDDRIVGAVLYGDTADGSWYFDLLKRQEDVSGLR
DLLIFGQAFAAGGGAADPKAAVAALSDDAEICGCNGVTKGQVVSCITKGAHSLDAVRSTCKASASCGSCTGLVETLLALT
LGDEVQAGPKTMCKCTSFGHDDVRREIVAQNMRSIPEVMQKLHWTTPDGCSSCRPALNYYLLCALPGDYVDDQQSRFVNE
RLHANIQKDGTYSVVPRMWGGLTNPTELRAIADVVEKYNAPMVKVTGGQRLDIFGIKKEDLPAVWADLNAAGMVSGHAYG
KALRTVKTCVGSEWCRFGTQDSTGLGVKIERMSWGSWMPHKFKIAVSGCPRNCAEATIKDFGVVCVDSGYELHIGGNGGI
HVRATDLLCKVATEQEAMDYCAAFIQLYREEARYLERTAPWIERVGLDYVKGRLLDDPAGREALRARFLHSQSFSQDDPW
AQRAEGAEAEHHAPMARFIPQEELA

Sequences:

>Translated_825_residues
MEHRPLSPDADTRAHLVVIGNGMAGCRAVEELLARDPARYRVTIFGAEPRVNYNRIMLSPVLAGEKSFEDIVINDADWYA
DNGIALVAGDPVVALDRAARTVTTRGGIVETYDKLVIATGSDPFIIPVPGKDLPGVIAFRDMDDVGTMLSAAEQGGSAVV
IGGGLLGLEAAHGLSLRGMKVTVLHLMPTLMERQLDEAAGWLLKQALEARGQTILTGADTAEIVGEVKVEGVKLKDGTLI
PADLVVMAVGIRPSIALARDAGLAVGRGILVDDHMVTSDPDILAVGECVEHDGQVYGLVAPLWDMCRSLADGLVERPSGY
RGSVTSTKLKVSGIDVFSAGDFSGGDGCEDIVLRDASRGVYKRVIVKDDRIVGAVLYGDTADGSWYFDLLKRQEDVSGLR
DLLIFGQAFAAGGGAADPKAAVAALSDDAEICGCNGVTKGQVVSCITKGAHSLDAVRSTCKASASCGSCTGLVETLLALT
LGDEVQAGPKTMCKCTSFGHDDVRREIVAQNMRSIPEVMQKLHWTTPDGCSSCRPALNYYLLCALPGDYVDDQQSRFVNE
RLHANIQKDGTYSVVPRMWGGLTNPTELRAIADVVEKYNAPMVKVTGGQRLDIFGIKKEDLPAVWADLNAAGMVSGHAYG
KALRTVKTCVGSEWCRFGTQDSTGLGVKIERMSWGSWMPHKFKIAVSGCPRNCAEATIKDFGVVCVDSGYELHIGGNGGI
HVRATDLLCKVATEQEAMDYCAAFIQLYREEARYLERTAPWIERVGLDYVKGRLLDDPAGREALRARFLHSQSFSQDDPW
AQRAEGAEAEHHAPMARFIPQEELA
>Mature_825_residues
MEHRPLSPDADTRAHLVVIGNGMAGCRAVEELLARDPARYRVTIFGAEPRVNYNRIMLSPVLAGEKSFEDIVINDADWYA
DNGIALVAGDPVVALDRAARTVTTRGGIVETYDKLVIATGSDPFIIPVPGKDLPGVIAFRDMDDVGTMLSAAEQGGSAVV
IGGGLLGLEAAHGLSLRGMKVTVLHLMPTLMERQLDEAAGWLLKQALEARGQTILTGADTAEIVGEVKVEGVKLKDGTLI
PADLVVMAVGIRPSIALARDAGLAVGRGILVDDHMVTSDPDILAVGECVEHDGQVYGLVAPLWDMCRSLADGLVERPSGY
RGSVTSTKLKVSGIDVFSAGDFSGGDGCEDIVLRDASRGVYKRVIVKDDRIVGAVLYGDTADGSWYFDLLKRQEDVSGLR
DLLIFGQAFAAGGGAADPKAAVAALSDDAEICGCNGVTKGQVVSCITKGAHSLDAVRSTCKASASCGSCTGLVETLLALT
LGDEVQAGPKTMCKCTSFGHDDVRREIVAQNMRSIPEVMQKLHWTTPDGCSSCRPALNYYLLCALPGDYVDDQQSRFVNE
RLHANIQKDGTYSVVPRMWGGLTNPTELRAIADVVEKYNAPMVKVTGGQRLDIFGIKKEDLPAVWADLNAAGMVSGHAYG
KALRTVKTCVGSEWCRFGTQDSTGLGVKIERMSWGSWMPHKFKIAVSGCPRNCAEATIKDFGVVCVDSGYELHIGGNGGI
HVRATDLLCKVATEQEAMDYCAAFIQLYREEARYLERTAPWIERVGLDYVKGRLLDDPAGREALRARFLHSQSFSQDDPW
AQRAEGAEAEHHAPMARFIPQEELA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=289, Percent_Identity=27.3356401384083, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI65787454, Length=289, Percent_Identity=27.3356401384083, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI226437568, Length=289, Percent_Identity=27.3356401384083, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI22202629, Length=272, Percent_Identity=28.3088235294118, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI4757732, Length=272, Percent_Identity=28.3088235294118, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1789765, Length=846, Percent_Identity=35.6973995271868, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789065, Length=335, Percent_Identity=28.0597014925373, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1788892, Length=246, Percent_Identity=31.7073170731707, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=435, Percent_Identity=23.448275862069, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281359715, Length=365, Percent_Identity=25.7534246575342, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI281359713, Length=365, Percent_Identity=25.7534246575342, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24639250, Length=365, Percent_Identity=25.7534246575342, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI18543267, Length=365, Percent_Identity=25.7534246575342, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24639252, Length=365, Percent_Identity=25.7534246575342, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24639257, Length=365, Percent_Identity=25.7534246575342, Blast_Score=100, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585130, Length=277, Percent_Identity=23.826714801444, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28573993, Length=300, Percent_Identity=27, Blast_Score=74, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581020, Length=300, Percent_Identity=26.3333333333333, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88543; Mature: 88543

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHRPLSPDADTRAHLVVIGNGMAGCRAVEELLARDPARYRVTIFGAEPRVNYNRIMLSP
CCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEE
VLAGEKSFEDIVINDADWYADNGIALVAGDPVVALDRAARTVTTRGGIVETYDKLVIATG
ECCCCCCCCEEEEECCCEECCCCEEEEECCCEEEEHHHHHEEECCCCEEEECCEEEEEEC
SDPFIIPVPGKDLPGVIAFRDMDDVGTMLSAAEQGGSAVVIGGGLLGLEAAHGLSLRGMK
CCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCEEEHHHHCCCEECCCE
VTVLHLMPTLMERQLDEAAGWLLKQALEARGQTILTGADTAEIVGEVKVEGVKLKDGTLI
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHEEEEEEEEEECCCCCC
PADLVVMAVGIRPSIALARDAGLAVGRGILVDDHMVTSDPDILAVGECVEHDGQVYGLVA
CHHHHHEEECCCCCEEEECCCCHHHCCCEEEECCEECCCCCEEEECHHHCCCCCEEEEHH
PLWDMCRSLADGLVERPSGYRGSVTSTKLKVSGIDVFSAGDFSGGDGCEDIVLRDASRGV
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECEEEEECCCCCCCCCCHHHHEECCCCCC
YKRVIVKDDRIVGAVLYGDTADGSWYFDLLKRQEDVSGLRDLLIFGQAFAAGGGAADPKA
EEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AVAALSDDAEICGCNGVTKGQVVSCITKGAHSLDAVRSTCKASASCGSCTGLVETLLALT
HHEEECCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LGDEVQAGPKTMCKCTSFGHDDVRREIVAQNMRSIPEVMQKLHWTTPDGCSSCRPALNYY
CCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCEE
LLCALPGDYVDDQQSRFVNERLHANIQKDGTYSVVPRMWGGLTNPTELRAIADVVEKYNA
EEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHCC
PMVKVTGGQRLDIFGIKKEDLPAVWADLNAAGMVSGHAYGKALRTVKTCVGSEWCRFGTQ
CEEEECCCCEEEEEECCCCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHHCCHHHHCCCC
DSTGLGVKIERMSWGSWMPHKFKIAVSGCPRNCAEATIKDFGVVCVDSGYELHIGGNGGI
CCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEECCCCEEEECCCCCE
HVRATDLLCKVATEQEAMDYCAAFIQLYREEARYLERTAPWIERVGLDYVKGRLLDDPAG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCC
REALRARFLHSQSFSQDDPWAQRAEGAEAEHHAPMARFIPQEELA
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHCCCHHHCCCHHHCC
>Mature Secondary Structure
MEHRPLSPDADTRAHLVVIGNGMAGCRAVEELLARDPARYRVTIFGAEPRVNYNRIMLSP
CCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEE
VLAGEKSFEDIVINDADWYADNGIALVAGDPVVALDRAARTVTTRGGIVETYDKLVIATG
ECCCCCCCCEEEEECCCEECCCCEEEEECCCEEEEHHHHHEEECCCCEEEECCEEEEEEC
SDPFIIPVPGKDLPGVIAFRDMDDVGTMLSAAEQGGSAVVIGGGLLGLEAAHGLSLRGMK
CCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCEEEHHHHCCCEECCCE
VTVLHLMPTLMERQLDEAAGWLLKQALEARGQTILTGADTAEIVGEVKVEGVKLKDGTLI
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHEEEEEEEEEECCCCCC
PADLVVMAVGIRPSIALARDAGLAVGRGILVDDHMVTSDPDILAVGECVEHDGQVYGLVA
CHHHHHEEECCCCCEEEECCCCHHHCCCEEEECCEECCCCCEEEECHHHCCCCCEEEEHH
PLWDMCRSLADGLVERPSGYRGSVTSTKLKVSGIDVFSAGDFSGGDGCEDIVLRDASRGV
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECEEEEECCCCCCCCCCHHHHEECCCCCC
YKRVIVKDDRIVGAVLYGDTADGSWYFDLLKRQEDVSGLRDLLIFGQAFAAGGGAADPKA
EEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AVAALSDDAEICGCNGVTKGQVVSCITKGAHSLDAVRSTCKASASCGSCTGLVETLLALT
HHEEECCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LGDEVQAGPKTMCKCTSFGHDDVRREIVAQNMRSIPEVMQKLHWTTPDGCSSCRPALNYY
CCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCEE
LLCALPGDYVDDQQSRFVNERLHANIQKDGTYSVVPRMWGGLTNPTELRAIADVVEKYNA
EEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHCC
PMVKVTGGQRLDIFGIKKEDLPAVWADLNAAGMVSGHAYGKALRTVKTCVGSEWCRFGTQ
CEEEECCCCEEEEEECCCCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHHCCHHHHCCCC
DSTGLGVKIERMSWGSWMPHKFKIAVSGCPRNCAEATIKDFGVVCVDSGYELHIGGNGGI
CCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEECCCCEEEECCCCCE
HVRATDLLCKVATEQEAMDYCAAFIQLYREEARYLERTAPWIERVGLDYVKGRLLDDPAG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCC
REALRARFLHSQSFSQDDPWAQRAEGAEAEHHAPMARFIPQEELA
HHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHCCCHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]