| Definition | Sphingopyxis alaskensis RB2256, complete genome. |
|---|---|
| Accession | NC_008048 |
| Length | 3,345,170 |
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The map label for this gene is topA [H]
Identifier: 103486699
GI number: 103486699
Start: 1251170
End: 1253719
Strand: Direct
Name: topA [H]
Synonym: Sala_1211
Alternate gene names: 103486699
Gene position: 1251170-1253719 (Clockwise)
Preceding gene: 103486698
Following gene: 103486703
Centisome position: 37.4
GC content: 64.67
Gene sequence:
>2550_bases ATGCAATTGGTAATTGTGGAATCGCCGGCAAAGGCGAAAACGATCGAGAAATATCTCGGTCGCGATTTCAAGGTGCTGGC CAGTTATGGCCATGTCCGCGACTTGCCGCCGAAGGACGGCTCGGTCGATCCCGACGACGGCTTTGCAATGCAGTGGCAGC TTTATCCCGACAAGGCGAAGCGGCTGAAGGAGATTTCGGACGCCGCCAAGGGCGCCGACCGCCTGATCCTCGCGACCGAC CCCGATCGCGAGGGCGAGGCGATCAGCTGGCATGTGCAGGAATTGCTGCGCAGCAAAAAGGCGCTGCCCGCGGCCGTCGA TCGCGTGACGTTCAATGCGATCACCAAGCAGGCGGTGACTGACGCGATGAGGCATCCGCGCGAGCTCGACACCGACCTGA TCGACGCCTATCGCGCACGCCGCGCGCTCGACTATCTGGTTGGTTTCACATTGTCGCCGGTGCTGTGGCGCAAGCTGCCC GGTGCCAAGTCGGCGGGGCGGGTCCAGTCGGTCGCGCTGCGCCTCGTCGTCGAACGCGAGCGCGAGATCGAGGCCTTTGT TGCGCAGCAATACTGGTCGGTGACCGGTCTGTTCGAAATGGGCGGCACGCCCTTCAAGGCGCGGCTGTCGCGTTGGAGGG GCGACAAGATCGAGCGGCTCTCGATCACCTCCGAAGCCGATGCGCGCGCGGCCGAAGCCGACGTGAAGGCGGGCCATTTC ACCGTCGATACGGTCGAGACGAAGCCCCTGACCCGCAACCCGCCGCCGCCCTTCACCACCTCGACCCTGCAACAGGAGGC TGCGCGCAAGCTGGGTTTTTCGGCGAGCCACACGATGCGCATCGCGCAGGCGCTCTACGAAGATGGCGCGATCACCTATA TGCGGACCGACGGCGTCCAGATGGACGGCAGCGCGATCAGCGCCGCGCGCAAGGCGATCGCGACGCGCTACGATGGCGGC TATGTCCCGGACAAGCCGCGGCAGTATCAGACCAAGGCGAAAAATGCGCAGGAAGCGCATGAGGCCATCCGCCCCACCGA TTTCGGCAAGGACAAGGTGGGCGGCGGTGACCATGCGCGGCTCTACGAGCTGATCTGGAAGCGCGCGCTCGCCAGCCAGA TGGCGTCGGCGCGGCTCGAACGCACCAGTGTCGAATTGAGCGATGGCACCGGCAAGACGACGCTGCGCGCGACGGGGCAG GTGGTGAAGTTTCCGGGCTTCCTTGCTCTCTATGATGAAGGCCGCGACGATGCAGAGGACGAGGATGCGCGCCTGCTGCC GGCGATGGCGAAGGGAGATGCGCCGGCGAAAACCGGCGTCGAAGTCGAGAGGCACGAAACGCAGCCTCCGCCACGCTATT CGGAAGCGAGCCTCGTCAAGAAGATGGAGGAACTCGGCATCGGGCGACCGTCGACCTATGCCTCGATCCTTCAGGTACTG AAGGATCGCGACTATGTGCGGGTCGAGAAGAACCGCTTCATTCCCGAAGAGAGTGGTCGGCTGCTCACCGCCTTCCTCGA ACGCTTCTTCGAACGTTATGTCGGCTATGATTTCACGGCGGAGCTTGAGGAAGAGCTGGACGACGTATCAGGCGGCCGCG CGCAGTGGCAGGCGGTGCTCGAACGCTTCTGGCGCGACTTCAAGCCGCGCACCGGCGAGGTGATGGAGCAGAAGCCATCG GAGATTACCGCGGCGCTCGACGAGTTCCTCGGTCCCTATCTCTTCCCCGACAAGAGCGACGGTAGCGACCCCCGGCTCTG CCCCAATTGCGGCACCGGGCGGTTGGCGCTGCGTGGCGGCAAGTTCGGGCCATTCATCGCGTGCAGCAACTATCCCGACT GCAAGTTCACGCGCAAATTCGCGCAGTCAGGTGGTAATGGCGAGGCGGATAACGGACCCGAGACGCTGGGCACCGATCCC GAAAGCGGGCTGGAGGTCACGAAGCGCAGCGGACGTTTCGGTCCATACATCCAGCTCGGCGAGGGGAGTGAGGCAAAACG CTCGTCGATCCCCAAGGACATCCCGGCGGAGGATTTCGACCTCGACTATGCGCTCCGGCTCCTCAGCCTGCCGCGCGAGG TCGGGGTGCATCCCGAAAGCGGCAAGCCGATCATGGCCGGCCTGGGTCGCTACGGCCCCTATCTGCTCCACGATGGCAAA TATGCGAAGCTGGGCGGCACCGCCGAAATCTTTGACATCGGCATGAACGCCGCGGTCGTCCGTATCGCCGAGGCGGCGAA TGGCGGCGGACGCGGGCGTGCAAAGGCCGAACCGCTCAACACCTTTGGCCCGCACCCGACGAGCGGCGGCGAGATGAAGC TGATGGAAGGGCGCTATGGTCCCTATGTCACCGACGGCACGACCAACGCCACCTTGCCCAAGACCACCGACCCGGCGACG CTGACGATGGAAGAGGCGATCGCGCTGATCGACGCACGCGCGGCGAAGGGCCCGGTGAAGGGCAAGAAGAAGGCGGCGCC GAAGAAAAAGGCGGCTGCGAAGAAAGCTGCGGCGAAGGAGGCACCCGCCAGGAAGACGGCGGCCGAGTAG
Upstream 100 bases:
>100_bases ATGGCAGAACGGGTGACGCAAAATCCGGCTTGACGGAATCAAGCTATCCCCGCCACCCTCGCGCGTACATGCGAGAGCCG GTGACGGGGATAGTTTTTTA
Downstream 100 bases:
>100_bases CGCCCTCCTTGATCGTCATTCCCGCGTTGGCGGGGATGACGAGGGCGGGGCGTCAATCCACCCAGTCAAGCCCCATGTCA CGATAGACGCTGCGGTCCTC
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 849; Mature: 849
Protein sequence:
>849_residues MQLVIVESPAKAKTIEKYLGRDFKVLASYGHVRDLPPKDGSVDPDDGFAMQWQLYPDKAKRLKEISDAAKGADRLILATD PDREGEAISWHVQELLRSKKALPAAVDRVTFNAITKQAVTDAMRHPRELDTDLIDAYRARRALDYLVGFTLSPVLWRKLP GAKSAGRVQSVALRLVVEREREIEAFVAQQYWSVTGLFEMGGTPFKARLSRWRGDKIERLSITSEADARAAEADVKAGHF TVDTVETKPLTRNPPPPFTTSTLQQEAARKLGFSASHTMRIAQALYEDGAITYMRTDGVQMDGSAISAARKAIATRYDGG YVPDKPRQYQTKAKNAQEAHEAIRPTDFGKDKVGGGDHARLYELIWKRALASQMASARLERTSVELSDGTGKTTLRATGQ VVKFPGFLALYDEGRDDAEDEDARLLPAMAKGDAPAKTGVEVERHETQPPPRYSEASLVKKMEELGIGRPSTYASILQVL KDRDYVRVEKNRFIPEESGRLLTAFLERFFERYVGYDFTAELEEELDDVSGGRAQWQAVLERFWRDFKPRTGEVMEQKPS EITAALDEFLGPYLFPDKSDGSDPRLCPNCGTGRLALRGGKFGPFIACSNYPDCKFTRKFAQSGGNGEADNGPETLGTDP ESGLEVTKRSGRFGPYIQLGEGSEAKRSSIPKDIPAEDFDLDYALRLLSLPREVGVHPESGKPIMAGLGRYGPYLLHDGK YAKLGGTAEIFDIGMNAAVVRIAEAANGGGRGRAKAEPLNTFGPHPTSGGEMKLMEGRYGPYVTDGTTNATLPKTTDPAT LTMEEAIALIDARAAKGPVKGKKKAAPKKKAAAKKAAAKEAPARKTAAE
Sequences:
>Translated_849_residues MQLVIVESPAKAKTIEKYLGRDFKVLASYGHVRDLPPKDGSVDPDDGFAMQWQLYPDKAKRLKEISDAAKGADRLILATD PDREGEAISWHVQELLRSKKALPAAVDRVTFNAITKQAVTDAMRHPRELDTDLIDAYRARRALDYLVGFTLSPVLWRKLP GAKSAGRVQSVALRLVVEREREIEAFVAQQYWSVTGLFEMGGTPFKARLSRWRGDKIERLSITSEADARAAEADVKAGHF TVDTVETKPLTRNPPPPFTTSTLQQEAARKLGFSASHTMRIAQALYEDGAITYMRTDGVQMDGSAISAARKAIATRYDGG YVPDKPRQYQTKAKNAQEAHEAIRPTDFGKDKVGGGDHARLYELIWKRALASQMASARLERTSVELSDGTGKTTLRATGQ VVKFPGFLALYDEGRDDAEDEDARLLPAMAKGDAPAKTGVEVERHETQPPPRYSEASLVKKMEELGIGRPSTYASILQVL KDRDYVRVEKNRFIPEESGRLLTAFLERFFERYVGYDFTAELEEELDDVSGGRAQWQAVLERFWRDFKPRTGEVMEQKPS EITAALDEFLGPYLFPDKSDGSDPRLCPNCGTGRLALRGGKFGPFIACSNYPDCKFTRKFAQSGGNGEADNGPETLGTDP ESGLEVTKRSGRFGPYIQLGEGSEAKRSSIPKDIPAEDFDLDYALRLLSLPREVGVHPESGKPIMAGLGRYGPYLLHDGK YAKLGGTAEIFDIGMNAAVVRIAEAANGGGRGRAKAEPLNTFGPHPTSGGEMKLMEGRYGPYVTDGTTNATLPKTTDPAT LTMEEAIALIDARAAKGPVKGKKKAAPKKKAAAKKAAAKEAPARKTAAE >Mature_849_residues MQLVIVESPAKAKTIEKYLGRDFKVLASYGHVRDLPPKDGSVDPDDGFAMQWQLYPDKAKRLKEISDAAKGADRLILATD PDREGEAISWHVQELLRSKKALPAAVDRVTFNAITKQAVTDAMRHPRELDTDLIDAYRARRALDYLVGFTLSPVLWRKLP GAKSAGRVQSVALRLVVEREREIEAFVAQQYWSVTGLFEMGGTPFKARLSRWRGDKIERLSITSEADARAAEADVKAGHF TVDTVETKPLTRNPPPPFTTSTLQQEAARKLGFSASHTMRIAQALYEDGAITYMRTDGVQMDGSAISAARKAIATRYDGG YVPDKPRQYQTKAKNAQEAHEAIRPTDFGKDKVGGGDHARLYELIWKRALASQMASARLERTSVELSDGTGKTTLRATGQ VVKFPGFLALYDEGRDDAEDEDARLLPAMAKGDAPAKTGVEVERHETQPPPRYSEASLVKKMEELGIGRPSTYASILQVL KDRDYVRVEKNRFIPEESGRLLTAFLERFFERYVGYDFTAELEEELDDVSGGRAQWQAVLERFWRDFKPRTGEVMEQKPS EITAALDEFLGPYLFPDKSDGSDPRLCPNCGTGRLALRGGKFGPFIACSNYPDCKFTRKFAQSGGNGEADNGPETLGTDP ESGLEVTKRSGRFGPYIQLGEGSEAKRSSIPKDIPAEDFDLDYALRLLSLPREVGVHPESGKPIMAGLGRYGPYLLHDGK YAKLGGTAEIFDIGMNAAVVRIAEAANGGGRGRAKAEPLNTFGPHPTSGGEMKLMEGRYGPYVTDGTTNATLPKTTDPAT LTMEEAIALIDARAAKGPVKGKKKAAPKKKAAAKKAAAKEAPARKTAAE
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=586, Percent_Identity=27.3037542662116, Blast_Score=142, Evalue=1e-33, Organism=Homo sapiens, GI4507635, Length=646, Percent_Identity=22.6006191950464, Blast_Score=114, Evalue=5e-25, Organism=Escherichia coli, GI1787529, Length=642, Percent_Identity=40.6542056074766, Blast_Score=442, Evalue=1e-125, Organism=Escherichia coli, GI1788061, Length=538, Percent_Identity=27.3234200743494, Blast_Score=123, Evalue=5e-29, Organism=Caenorhabditis elegans, GI32563869, Length=541, Percent_Identity=28.0961182994455, Blast_Score=152, Evalue=5e-37, Organism=Caenorhabditis elegans, GI17555378, Length=500, Percent_Identity=28.6, Blast_Score=141, Evalue=2e-33, Organism=Saccharomyces cerevisiae, GI6323263, Length=491, Percent_Identity=27.0875763747454, Blast_Score=119, Evalue=2e-27, Organism=Drosophila melanogaster, GI24585251, Length=585, Percent_Identity=28.034188034188, Blast_Score=157, Evalue=3e-38, Organism=Drosophila melanogaster, GI24640096, Length=556, Percent_Identity=25.8992805755396, Blast_Score=117, Evalue=3e-26,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 93070; Mature: 93070
Theoretical pI: Translated: 8.78; Mature: 8.78
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQLVIVESPAKAKTIEKYLGRDFKVLASYGHVRDLPPKDGSVDPDDGFAMQWQLYPDKAK CEEEEECCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH RLKEISDAAKGADRLILATDPDREGEAISWHVQELLRSKKALPAAVDRVTFNAITKQAVT HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH DAMRHPRELDTDLIDAYRARRALDYLVGFTLSPVLWRKLPGAKSAGRVQSVALRLVVERE HHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH REIEAFVAQQYWSVTGLFEMGGTPFKARLSRWRGDKIERLSITSEADARAAEADVKAGHF HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCE TVDTVETKPLTRNPPPPFTTSTLQQEAARKLGFSASHTMRIAQALYEDGAITYMRTDGVQ EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCE MDGSAISAARKAIATRYDGGYVPDKPRQYQTKAKNAQEAHEAIRPTDFGKDKVGGGDHAR ECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHH LYELIWKRALASQMASARLERTSVELSDGTGKTTLRATGQVVKFPGFLALYDEGRDDAED HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCEEECCCEEEEECCCCCCCCC EDARLLPAMAKGDAPAKTGVEVERHETQPPPRYSEASLVKKMEELGIGRPSTYASILQVL CCCCCCHHHHCCCCCCCCCCEEHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH KDRDYVRVEKNRFIPEESGRLLTAFLERFFERYVGYDFTAELEEELDDVSGGRAQWQAVL HCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH ERFWRDFKPRTGEVMEQKPSEITAALDEFLGPYLFPDKSDGSDPRLCPNCGTGRLALRGG HHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECC KFGPFIACSNYPDCKFTRKFAQSGGNGEADNGPETLGTDPESGLEVTKRSGRFGPYIQLG CCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEC EGSEAKRSSIPKDIPAEDFDLDYALRLLSLPREVGVHPESGKPIMAGLGRYGPYLLHDGK CCCCHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHCCCCCCCCHHHHHHCCCCCEEEECCC YAKLGGTAEIFDIGMNAAVVRIAEAANGGGRGRAKAEPLNTFGPHPTSGGEMKLMEGRYG EEECCCCCEEECCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC PYVTDGTTNATLPKTTDPATLTMEEAIALIDARAAKGPVKGKKKAAPKKKAAAKKAAAKE CEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHH APARKTAAE CCCHHCCCC >Mature Secondary Structure MQLVIVESPAKAKTIEKYLGRDFKVLASYGHVRDLPPKDGSVDPDDGFAMQWQLYPDKAK CEEEEECCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH RLKEISDAAKGADRLILATDPDREGEAISWHVQELLRSKKALPAAVDRVTFNAITKQAVT HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH DAMRHPRELDTDLIDAYRARRALDYLVGFTLSPVLWRKLPGAKSAGRVQSVALRLVVERE HHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH REIEAFVAQQYWSVTGLFEMGGTPFKARLSRWRGDKIERLSITSEADARAAEADVKAGHF HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCE TVDTVETKPLTRNPPPPFTTSTLQQEAARKLGFSASHTMRIAQALYEDGAITYMRTDGVQ EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCE MDGSAISAARKAIATRYDGGYVPDKPRQYQTKAKNAQEAHEAIRPTDFGKDKVGGGDHAR ECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHH LYELIWKRALASQMASARLERTSVELSDGTGKTTLRATGQVVKFPGFLALYDEGRDDAED HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCEEECCCEEEEECCCCCCCCC EDARLLPAMAKGDAPAKTGVEVERHETQPPPRYSEASLVKKMEELGIGRPSTYASILQVL CCCCCCHHHHCCCCCCCCCCEEHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH KDRDYVRVEKNRFIPEESGRLLTAFLERFFERYVGYDFTAELEEELDDVSGGRAQWQAVL HCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH ERFWRDFKPRTGEVMEQKPSEITAALDEFLGPYLFPDKSDGSDPRLCPNCGTGRLALRGG HHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECC KFGPFIACSNYPDCKFTRKFAQSGGNGEADNGPETLGTDPESGLEVTKRSGRFGPYIQLG CCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEC EGSEAKRSSIPKDIPAEDFDLDYALRLLSLPREVGVHPESGKPIMAGLGRYGPYLLHDGK CCCCHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHCCCCCCCCHHHHHHCCCCCEEEECCC YAKLGGTAEIFDIGMNAAVVRIAEAANGGGRGRAKAEPLNTFGPHPTSGGEMKLMEGRYG EEECCCCCEEECCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC PYVTDGTTNATLPKTTDPATLTMEEAIALIDARAAKGPVKGKKKAAPKKKAAAKKAAAKE CEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHH APARKTAAE CCCHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA