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The map label for this gene is psdht [H]

Identifier: 103486690

GI number: 103486690

Start: 1243095

End: 1244096

Strand: Direct

Name: psdht [H]

Synonym: Sala_1202

Alternate gene names: 103486690

Gene position: 1243095-1244096 (Clockwise)

Preceding gene: 103486680

Following gene: 103486691

Centisome position: 37.16

GC content: 69.46

Gene sequence:

>1002_bases
ATGATACAGCAAAGCCCCGATCCTGCGCTCGCCCCGCTGCTCGACCCGAACCGCGCCCCCTCGCTCGCAGGGGTGGAACG
CGCTGCGGCCAAGGTTGCTGCCTTGCTCCCGCAGACGCCGCTGCTGCCGCTCGAGGTCGACGGCCGCACCATCTGGTGCA
AAGCCGAATCGCTCCAGCCCGTCGGCGCGTTCAAGATCCGCGGCGCGTGGCACCGGCTGACCGACCTGACGCCCGAACAG
GCGGCGGCGGGCGTGGTCGGTGTGTCCAGCGGCAATCATGCGCAGGGGGTCGCCTGGGCGGCGAAGCGGCTCGGTATCCG
CGCCACGATCGTCATGCCGGGCAACGCCCCCGCGATGAAACTCGCAGCGACGCGCAGGCTGGGAGCGGAAGTCGTGCTCT
ATGACCGCGTTACCGAATCGCGCGATGCCGTCGCGGCAAAGCTGCTCGCCGAGCGCGGCGGGACGCTGGTTCATGCCTAT
GGTGATCCGTGGATCATCGAGGGGCAGGGGAGCGCGGGGATCGAGGCGGCGATGCAGATGCGCGCACGCGGGATCGACGG
CCCCGACCGGATCGTCGCCTGTTGCGGCGGCGGCGGGCTGTCGGCCGGGCTGGCGCTCGCCTGCCCCGACGCACAGGTGA
TCGCCGTCGAGCCTGAGGGGTGGGATGATGTGACGCGCAGTCTGGCCGCGGGAGAGATACTGAGCGTCGAGGATATGTCC
TTTCCTACCGAATGCGACGCGCTCCAGACGCCCGAAACATGGCCGATCAACTTTGCGGTGCTTCGGGCGCGCGGTGTCCG
CGGGGTCGTCGTGACGCGCAGCGAAGTGCGCGACGCGATGCGCCTCGCCTTTGAAAGGCTGCACCTGGTCATCGAACCCG
GCGGCGCGGCGGCGCTGGCGGCAGTGCTGGCGGGCAAGGTGACGCCGACCGACGCGACGCTCGTCACCCTGTCAGGCGGC
AATGTCGATCCGCTGCAATTCGCCGAAATCATCAGCGAATAG

Upstream 100 bases:

>100_bases
CGCCGCCTGCGCCGATCACCAGAACCTTGCTCATCGAAAAATCTCCTGTCGCGGACCGTTGCCGCCTTGTCTTGTATGCC
CGGCCCTATAGGACGAAGCC

Downstream 100 bases:

>100_bases
GAAACTTTGCGCTCATCGGGCGTTTGCGTCGCAAAGGAGAGAGACGATGCACAAGAGTCTGACGATGGCGGCGGCTTCTG
CCGCGATGCTGCTCACCGGC

Product: pyridoxal-5'-phosphate-dependent enzyme, beta subunit

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MIQQSPDPALAPLLDPNRAPSLAGVERAAAKVAALLPQTPLLPLEVDGRTIWCKAESLQPVGAFKIRGAWHRLTDLTPEQ
AAAGVVGVSSGNHAQGVAWAAKRLGIRATIVMPGNAPAMKLAATRRLGAEVVLYDRVTESRDAVAAKLLAERGGTLVHAY
GDPWIIEGQGSAGIEAAMQMRARGIDGPDRIVACCGGGGLSAGLALACPDAQVIAVEPEGWDDVTRSLAAGEILSVEDMS
FPTECDALQTPETWPINFAVLRARGVRGVVVTRSEVRDAMRLAFERLHLVIEPGGAAALAAVLAGKVTPTDATLVTLSGG
NVDPLQFAEIISE

Sequences:

>Translated_333_residues
MIQQSPDPALAPLLDPNRAPSLAGVERAAAKVAALLPQTPLLPLEVDGRTIWCKAESLQPVGAFKIRGAWHRLTDLTPEQ
AAAGVVGVSSGNHAQGVAWAAKRLGIRATIVMPGNAPAMKLAATRRLGAEVVLYDRVTESRDAVAAKLLAERGGTLVHAY
GDPWIIEGQGSAGIEAAMQMRARGIDGPDRIVACCGGGGLSAGLALACPDAQVIAVEPEGWDDVTRSLAAGEILSVEDMS
FPTECDALQTPETWPINFAVLRARGVRGVVVTRSEVRDAMRLAFERLHLVIEPGGAAALAAVLAGKVTPTDATLVTLSGG
NVDPLQFAEIISE
>Mature_333_residues
MIQQSPDPALAPLLDPNRAPSLAGVERAAAKVAALLPQTPLLPLEVDGRTIWCKAESLQPVGAFKIRGAWHRLTDLTPEQ
AAAGVVGVSSGNHAQGVAWAAKRLGIRATIVMPGNAPAMKLAATRRLGAEVVLYDRVTESRDAVAAKLLAERGGTLVHAY
GDPWIIEGQGSAGIEAAMQMRARGIDGPDRIVACCGGGGLSAGLALACPDAQVIAVEPEGWDDVTRSLAAGEILSVEDMS
FPTECDALQTPETWPINFAVLRARGVRGVVVTRSEVRDAMRLAFERLHLVIEPGGAAALAAVLAGKVTPTDATLVTLSGG
NVDPLQFAEIISE

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=323, Percent_Identity=34.984520123839, Blast_Score=151, Evalue=8e-37,
Organism=Escherichia coli, GI1789505, Length=313, Percent_Identity=32.5878594249201, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790207, Length=321, Percent_Identity=33.0218068535826, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789235, Length=188, Percent_Identity=29.2553191489362, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508781, Length=282, Percent_Identity=32.2695035460993, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71991565, Length=335, Percent_Identity=28.955223880597, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17537387, Length=289, Percent_Identity=33.9100346020761, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322631, Length=321, Percent_Identity=32.7102803738318, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6320930, Length=291, Percent_Identity=32.6460481099656, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21355833, Length=230, Percent_Identity=30.8695652173913, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 34687; Mature: 34687

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQQSPDPALAPLLDPNRAPSLAGVERAAAKVAALLPQTPLLPLEVDGRTIWCKAESLQP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCC
VGAFKIRGAWHRLTDLTPEQAAAGVVGVSSGNHAQGVAWAAKRLGIRATIVMPGNAPAMK
CCEEEECCHHHHHHCCCHHHHHCCEEEECCCCCCCHHHHHHHHCCEEEEEEECCCCCHHH
LAATRRLGAEVVLYDRVTESRDAVAAKLLAERGGTLVHAYGDPWIIEGQGSAGIEAAMQM
HHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCHHHHHHH
RARGIDGPDRIVACCGGGGLSAGLALACPDAQVIAVEPEGWDDVTRSLAAGEILSVEDMS
HHCCCCCHHHEEEEECCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHCCCEEEECCCC
FPTECDALQTPETWPINFAVLRARGVRGVVVTRSEVRDAMRLAFERLHLVIEPGGAAALA
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHEEEEECCCCHHHHH
AVLAGKVTPTDATLVTLSGGNVDPLQFAEIISE
HHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MIQQSPDPALAPLLDPNRAPSLAGVERAAAKVAALLPQTPLLPLEVDGRTIWCKAESLQP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCC
VGAFKIRGAWHRLTDLTPEQAAAGVVGVSSGNHAQGVAWAAKRLGIRATIVMPGNAPAMK
CCEEEECCHHHHHHCCCHHHHHCCEEEECCCCCCCHHHHHHHHCCEEEEEEECCCCCHHH
LAATRRLGAEVVLYDRVTESRDAVAAKLLAERGGTLVHAYGDPWIIEGQGSAGIEAAMQM
HHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCHHHHHHH
RARGIDGPDRIVACCGGGGLSAGLALACPDAQVIAVEPEGWDDVTRSLAAGEILSVEDMS
HHCCCCCHHHEEEEECCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHCCCEEEECCCC
FPTECDALQTPETWPINFAVLRARGVRGVVVTRSEVRDAMRLAFERLHLVIEPGGAAALA
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHEEEEECCCCHHHHH
AVLAGKVTPTDATLVTLSGGNVDPLQFAEIISE
HHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]