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The map label for this gene is ispE

Identifier: 103486675

GI number: 103486675

Start: 1227605

End: 1228456

Strand: Direct

Name: ispE

Synonym: Sala_1187

Alternate gene names: 103486675

Gene position: 1227605-1228456 (Clockwise)

Preceding gene: 103486674

Following gene: 103486676

Centisome position: 36.7

GC content: 68.78

Gene sequence:

>852_bases
ATGAGTGCCACGATGCGGGAGACGGGCTGGGCCAAGATAAACCTTGCGCTCCACGTCCGCGCGCGGCGCGCCGACGGCTA
TCACGATATTGAAACGCTGTTTGCCTTCGTCGATGGCGGCGATACGATCGAAGCCGCCCTTGCCGCCACCGACACGCTTG
TCATCGACGGTGAGTTTGCCGAAGGCCTGAGTTCGGGCGCCGACAATCTGGTGCTGCGCGTACTTGCGCTGCTGCGCGCA
CGCTATGGCGCCGATCGCGTGCCGCCGCTCGCGGTAAGGCTGACGAAGCGTTTGCCGTTGGCGGCGGGGATCGGCGGCGG
CTCCGCCGATGCCGCGGCGATGGCGCGGCTGGTCCGCACGCATTTCCTTCCCGAACTGGGTGATGCGACGCTCGCGCGCA
TCGTCGGTCCGCTCGGTGCTGATATTGCCGCCTGCGTGGCGAGCACGACCTGCATGGGTTCGGGGACCGGCGAGGATCTG
CACGCCGTCGCGGGTCTGCGGATCGCGGGCGTACCGGTGCTGCTGGTCAATCCGCGCCAGCCCGTCGCGACAGGGCCGGT
CTTTGCCGCGTGGGACGGGATCGACCGCGGGCCGCTCTACATTGGCACGGACTACCGGGCGCAGTTGATCGGCGCGCGCA
ACGATTTGCAGCGCCCCGCGCTCGCCGCCTGCCCTGCGATTTCCGACATATTGCTTGAACTCGGCGCGCTGCGACCGTGG
CTCGCGCGCATGTCGGGATCGGGAGCGACCTGCTTCGCGCTGTTCGATGCGGCGGCCGACCGCGACGCGGCAAAGGCAGT
GCTCGCCGCACGGCATCCGGGATGGTGGCTGATGGCAGGCGCGCTGCGATGA

Upstream 100 bases:

>100_bases
GCTATAACTGGCGCGCGGCAGCGCTGCTCGCCGACGCCGAGATGGCGACGCGGATCGAGGCGAAGCTGCGCGACGGGCTG
ACCCCGGCAACGGTGGCACC

Downstream 100 bases:

>100_bases
GCGAGGCGTGGCACCAACTGGGCGAGCCGCGCGCCGACGGCATATTGTTGATCGGGGATCATGCGTCGGCGTATGTCCCG
AACGACATCGACCTGGGCAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSATMRETGWAKINLALHVRARRADGYHDIETLFAFVDGGDTIEAALAATDTLVIDGEFAEGLSSGADNLVLRVLALLRA
RYGADRVPPLAVRLTKRLPLAAGIGGGSADAAAMARLVRTHFLPELGDATLARIVGPLGADIAACVASTTCMGSGTGEDL
HAVAGLRIAGVPVLLVNPRQPVATGPVFAAWDGIDRGPLYIGTDYRAQLIGARNDLQRPALAACPAISDILLELGALRPW
LARMSGSGATCFALFDAAADRDAAKAVLAARHPGWWLMAGALR

Sequences:

>Translated_283_residues
MSATMRETGWAKINLALHVRARRADGYHDIETLFAFVDGGDTIEAALAATDTLVIDGEFAEGLSSGADNLVLRVLALLRA
RYGADRVPPLAVRLTKRLPLAAGIGGGSADAAAMARLVRTHFLPELGDATLARIVGPLGADIAACVASTTCMGSGTGEDL
HAVAGLRIAGVPVLLVNPRQPVATGPVFAAWDGIDRGPLYIGTDYRAQLIGARNDLQRPALAACPAISDILLELGALRPW
LARMSGSGATCFALFDAAADRDAAKAVLAARHPGWWLMAGALR
>Mature_282_residues
SATMRETGWAKINLALHVRARRADGYHDIETLFAFVDGGDTIEAALAATDTLVIDGEFAEGLSSGADNLVLRVLALLRAR
YGADRVPPLAVRLTKRLPLAAGIGGGSADAAAMARLVRTHFLPELGDATLARIVGPLGADIAACVASTTCMGSGTGEDLH
AVAGLRIAGVPVLLVNPRQPVATGPVFAAWDGIDRGPLYIGTDYRAQLIGARNDLQRPALAACPAISDILLELGALRPWL
ARMSGSGATCFALFDAAADRDAAKAVLAARHPGWWLMAGALR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=294, Percent_Identity=30.952380952381, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_SPHAL (Q1GTW9)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616236.1
- ProteinModelPortal:   Q1GTW9
- SMR:   Q1GTW9
- GeneID:   4080835
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1187
- NMPDR:   fig|317655.9.peg.1121
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- ProtClustDB:   PRK14608
- BioCyc:   SALA317655:SALA_1187-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29450; Mature: 29319

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATMRETGWAKINLALHVRARRADGYHDIETLFAFVDGGDTIEAALAATDTLVIDGEFA
CCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEECCHHH
EGLSSGADNLVLRVLALLRARYGADRVPPLAVRLTKRLPLAAGIGGGSADAAAMARLVRT
HHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHH
HFLPELGDATLARIVGPLGADIAACVASTTCMGSGTGEDLHAVAGLRIAGVPVLLVNPRQ
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHCEEECCCCEEEECCCC
PVATGPVFAAWDGIDRGPLYIGTDYRAQLIGARNDLQRPALAACPAISDILLELGALRPW
CCCCCCEEEEECCCCCCCEEEECCHHHEEECCHHHHCCCHHHHHHHHHHHHHHHHCCCHH
LARMSGSGATCFALFDAAADRDAAKAVLAARHPGWWLMAGALR
HHHHCCCCCEEEEEEHHHCCHHHHHHHHHHCCCCCEEEECCCC
>Mature Secondary Structure 
SATMRETGWAKINLALHVRARRADGYHDIETLFAFVDGGDTIEAALAATDTLVIDGEFA
CCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEECCHHH
EGLSSGADNLVLRVLALLRARYGADRVPPLAVRLTKRLPLAAGIGGGSADAAAMARLVRT
HHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHH
HFLPELGDATLARIVGPLGADIAACVASTTCMGSGTGEDLHAVAGLRIAGVPVLLVNPRQ
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHCEEECCCCEEEECCCC
PVATGPVFAAWDGIDRGPLYIGTDYRAQLIGARNDLQRPALAACPAISDILLELGALRPW
CCCCCCEEEEECCCCCCCEEEECCHHHEEECCHHHHCCCHHHHHHHHHHHHHHHHCCCHH
LARMSGSGATCFALFDAAADRDAAKAVLAARHPGWWLMAGALR
HHHHCCCCCEEEEEEHHHCCHHHHHHHHHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA