Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 103486671

GI number: 103486671

Start: 1221629

End: 1223395

Strand: Reverse

Name: slt [H]

Synonym: Sala_1183

Alternate gene names: 103486671

Gene position: 1223395-1221629 (Counterclockwise)

Preceding gene: 103486672

Following gene: 103486670

Centisome position: 36.57

GC content: 68.19

Gene sequence:

>1767_bases
ATGCCGATTGTGACCAGATTCCTGTTTTCCTGCGCCGCTGCGACGGTCGCAATTGCCGCCGTTCCGGCGATGGCAGCCGA
TGCCGATGGTCCCCGTTTCGCCGCAGCGCCGCAGACGGTGCCCGGCATATTGTCGGCGAAGCAGAAACAGCTTTACACGC
AAATCCTTGCGGCGATCCGCGCCGAACGATGGAGCGACGCCCGAACGCTGCTCGACAGCGCCGACGCGGAGCCCCTCACC
GATTTCCTGCGTGCCGAACTGCTGGTCGCCGCGAACAGCCCGCGCGCCGAAGTCGCCGACATCATGCCGATTCTCGCGCG
GTCGCCATTTTTGCCGCAGGCCGCCCAGCTTGGTCGGTTGGCGGCAAAGCGCGGCGCGACCGACATTCCGGCGCTTCCCG
CCGAAACGCGCCTCGCCTGGGCCGGTAGCGCGCCGCGCCGCCTCGGCGCCGACGCCGTGAACGGCGATCCGGTTGCCGCC
AGCCTGGCGCGCACAATCCCCGATCGAATCAAGAATGACGATCCTGCGGGCGCCGAAGCCCTGCTCAACGCGGTCATCGA
CCAGCTGAGTCCCGAAGCGCGGGCCGAATGGCGGCAAAAGGTCGCCTGGTCCTATTATATCGAGAATGACGACCGCAACG
CGCTCCGGCTCTCGCTCGCCTGCACCGCCGATGGCGGGCCCTGGGCGACGCAGGGCGCCTGGGTGCAGGGCCTCGCGGCA
TGGCGGCTGAACGATTATCGCACCGCGCTCGCTGCCTTCGATCGCGTGGCCAGGGAAGCCCCCGACAGCGAATTGCGCGC
CGCGGCTCATTATTGGGCCGCCCGCGCCGCCGTCGCGGCCGGCGAACCCGCGGCGGTCCAGTCGCGCCTCCGCGCGGCTG
CGGCCAATGAGGAAACGCTTTACGGCCTGCTTGCGTCCGAGACGCTCGGCGTCGAGCCAAAGAGCCAGCGCGAGGCGCTC
CGTGCCGACCGCAGCGCATGGCGCGCGCTTCAGGGGCTCCCCAATGTAAGGCTCGCGATGGCGCTCGCCGAGATCGGCGA
GGAAAACTACGCCGGTGAAGCGCTACGCCACCAGGCGCGCATCGGCAATCCCGATCATCACGACGAACTGATCGCAATGG
CGGGCGCGCTCAGCCTGCCCGAAACGCAGCTCTACCTCGCGCACAACACGCCGCACGGCCGCAAGCCCGCCCCGACCGCG
CGGTATCCGCAACCGAAGTGGGAACCGAGCGGCGGCTGGCGCGTCGATCCCGCGCTTGTTTTTGCCCATACGCTTCAGGA
ATCGCGTTTCCAGCGCACCGCGGTCAGCAGCGCGGGTGCAACGGGACTGATGCAGGTACGTCCGGGCACCGCGCGCGACA
TCGTCCGATGGGAGGGTGCGGTCGCGGCGCTCGACGATCTCAAGACACCTGCGGCGAACATGGACCTCGGCCAACGTTAT
CTGCAGTATCTGAGCCGCGAGCCCGCGACGGGCGGCCTGCTGCCCAAGGTCATCGCGGCCTATAACGCCGGTCCCGCTCC
GGTCGCGCGCTGGCAGAGCGAAATTCGCGATAATGGCGACCCGCTGCTCTACATCGAATCGATCCCCTATTGGGAAACGC
GCGGCTATGTCGGCATCGTGCTGCGCAACTACTGGATGTACGAAGCACAGCAGGGCAAGCCGTCGGACAGCCGCGCGGCG
CTGGCGCAGGGCAAATGGCCCCGCTTTCCCGATGGCAAGGATCGCACGCGCCTGACCTGGGCTGGAGGCCTCGCCGATGC
CGATTGA

Upstream 100 bases:

>100_bases
ACCGTAGCCGTCGCTCATCCCGCGATGCTGCTGGCCCGCCCCGCGCAGAAGGCGGCGGCCTGGGATAGCCTGAAACTGTT
CAACCGGGGACGTTGACAGA

Downstream 100 bases:

>100_bases
CGAGAGCCTGCCGCTCAAGCCCGTTCGCATCGCGCTGCTGACGATTTCGGACAGCCGCAGCGCCGCCGATGACCGGTCGG
GCGACAGGCTCGAGGAACTG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MPIVTRFLFSCAAATVAIAAVPAMAADADGPRFAAAPQTVPGILSAKQKQLYTQILAAIRAERWSDARTLLDSADAEPLT
DFLRAELLVAANSPRAEVADIMPILARSPFLPQAAQLGRLAAKRGATDIPALPAETRLAWAGSAPRRLGADAVNGDPVAA
SLARTIPDRIKNDDPAGAEALLNAVIDQLSPEARAEWRQKVAWSYYIENDDRNALRLSLACTADGGPWATQGAWVQGLAA
WRLNDYRTALAAFDRVAREAPDSELRAAAHYWAARAAVAAGEPAAVQSRLRAAAANEETLYGLLASETLGVEPKSQREAL
RADRSAWRALQGLPNVRLAMALAEIGEENYAGEALRHQARIGNPDHHDELIAMAGALSLPETQLYLAHNTPHGRKPAPTA
RYPQPKWEPSGGWRVDPALVFAHTLQESRFQRTAVSSAGATGLMQVRPGTARDIVRWEGAVAALDDLKTPAANMDLGQRY
LQYLSREPATGGLLPKVIAAYNAGPAPVARWQSEIRDNGDPLLYIESIPYWETRGYVGIVLRNYWMYEAQQGKPSDSRAA
LAQGKWPRFPDGKDRTRLTWAGGLADAD

Sequences:

>Translated_588_residues
MPIVTRFLFSCAAATVAIAAVPAMAADADGPRFAAAPQTVPGILSAKQKQLYTQILAAIRAERWSDARTLLDSADAEPLT
DFLRAELLVAANSPRAEVADIMPILARSPFLPQAAQLGRLAAKRGATDIPALPAETRLAWAGSAPRRLGADAVNGDPVAA
SLARTIPDRIKNDDPAGAEALLNAVIDQLSPEARAEWRQKVAWSYYIENDDRNALRLSLACTADGGPWATQGAWVQGLAA
WRLNDYRTALAAFDRVAREAPDSELRAAAHYWAARAAVAAGEPAAVQSRLRAAAANEETLYGLLASETLGVEPKSQREAL
RADRSAWRALQGLPNVRLAMALAEIGEENYAGEALRHQARIGNPDHHDELIAMAGALSLPETQLYLAHNTPHGRKPAPTA
RYPQPKWEPSGGWRVDPALVFAHTLQESRFQRTAVSSAGATGLMQVRPGTARDIVRWEGAVAALDDLKTPAANMDLGQRY
LQYLSREPATGGLLPKVIAAYNAGPAPVARWQSEIRDNGDPLLYIESIPYWETRGYVGIVLRNYWMYEAQQGKPSDSRAA
LAQGKWPRFPDGKDRTRLTWAGGLADAD
>Mature_587_residues
PIVTRFLFSCAAATVAIAAVPAMAADADGPRFAAAPQTVPGILSAKQKQLYTQILAAIRAERWSDARTLLDSADAEPLTD
FLRAELLVAANSPRAEVADIMPILARSPFLPQAAQLGRLAAKRGATDIPALPAETRLAWAGSAPRRLGADAVNGDPVAAS
LARTIPDRIKNDDPAGAEALLNAVIDQLSPEARAEWRQKVAWSYYIENDDRNALRLSLACTADGGPWATQGAWVQGLAAW
RLNDYRTALAAFDRVAREAPDSELRAAAHYWAARAAVAAGEPAAVQSRLRAAAANEETLYGLLASETLGVEPKSQREALR
ADRSAWRALQGLPNVRLAMALAEIGEENYAGEALRHQARIGNPDHHDELIAMAGALSLPETQLYLAHNTPHGRKPAPTAR
YPQPKWEPSGGWRVDPALVFAHTLQESRFQRTAVSSAGATGLMQVRPGTARDIVRWEGAVAALDDLKTPAANMDLGQRYL
QYLSREPATGGLLPKVIAAYNAGPAPVARWQSEIRDNGDPLLYIESIPYWETRGYVGIVLRNYWMYEAQQGKPSDSRAAL
AQGKWPRFPDGKDRTRLTWAGGLADAD

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=134, Percent_Identity=35.0746268656716, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 63531; Mature: 63400

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIVTRFLFSCAAATVAIAAVPAMAADADGPRFAAAPQTVPGILSAKQKQLYTQILAAIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AERWSDARTLLDSADAEPLTDFLRAELLVAANSPRAEVADIMPILARSPFLPQAAQLGRL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
AAKRGATDIPALPAETRLAWAGSAPRRLGADAVNGDPVAASLARTIPDRIKNDDPAGAEA
HHHCCCCCCCCCCCCCEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
LLNAVIDQLSPEARAEWRQKVAWSYYIENDDRNALRLSLACTADGGPWATQGAWVQGLAA
HHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
WRLNDYRTALAAFDRVAREAPDSELRAAAHYWAARAAVAAGEPAAVQSRLRAAAANEETL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
YGLLASETLGVEPKSQREALRADRSAWRALQGLPNVRLAMALAEIGEENYAGEALRHQAR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
IGNPDHHDELIAMAGALSLPETQLYLAHNTPHGRKPAPTARYPQPKWEPSGGWRVDPALV
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
FAHTLQESRFQRTAVSSAGATGLMQVRPGTARDIVRWEGAVAALDDLKTPAANMDLGQRY
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHH
LQYLSREPATGGLLPKVIAAYNAGPAPVARWQSEIRDNGDPLLYIESIPYWETRGYVGIV
HHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
LRNYWMYEAQQGKPSDSRAALAQGKWPRFPDGKDRTRLTWAGGLADAD
EEHHEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
PIVTRFLFSCAAATVAIAAVPAMAADADGPRFAAAPQTVPGILSAKQKQLYTQILAAIR
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AERWSDARTLLDSADAEPLTDFLRAELLVAANSPRAEVADIMPILARSPFLPQAAQLGRL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
AAKRGATDIPALPAETRLAWAGSAPRRLGADAVNGDPVAASLARTIPDRIKNDDPAGAEA
HHHCCCCCCCCCCCCCEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
LLNAVIDQLSPEARAEWRQKVAWSYYIENDDRNALRLSLACTADGGPWATQGAWVQGLAA
HHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
WRLNDYRTALAAFDRVAREAPDSELRAAAHYWAARAAVAAGEPAAVQSRLRAAAANEETL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
YGLLASETLGVEPKSQREALRADRSAWRALQGLPNVRLAMALAEIGEENYAGEALRHQAR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
IGNPDHHDELIAMAGALSLPETQLYLAHNTPHGRKPAPTARYPQPKWEPSGGWRVDPALV
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
FAHTLQESRFQRTAVSSAGATGLMQVRPGTARDIVRWEGAVAALDDLKTPAANMDLGQRY
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCHHHHHHHCCCCCCCCHHHHHH
LQYLSREPATGGLLPKVIAAYNAGPAPVARWQSEIRDNGDPLLYIESIPYWETRGYVGIV
HHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
LRNYWMYEAQQGKPSDSRAALAQGKWPRFPDGKDRTRLTWAGGLADAD
EEHHEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]