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The map label for this gene is crp [H]

Identifier: 103486657

GI number: 103486657

Start: 1203705

End: 1204388

Strand: Reverse

Name: crp [H]

Synonym: Sala_1169

Alternate gene names: 103486657

Gene position: 1204388-1203705 (Counterclockwise)

Preceding gene: 103486658

Following gene: 103486653

Centisome position: 36.0

GC content: 64.47

Gene sequence:

>684_bases
ATGGATCACGCAAGACTTGCCGAACTGCAGAGCCCCGACAGCATCTTTTCCGGCCTTTCGGTCGAGGATTGGGCCGAAAT
CACCCGCCGCGCGGTTCAGGTCAATTTCGCCAAGGGCAAGGAATTGCTCGTCCAGGGCGATCCGGGCGACATGATGCTGA
TCCTGACCGAGGGGACCGCGCGCGTGTCGATGCTGACGGCCGGCGGGCGCGAAATCGTCCTCGCTTATGCCGAACCCGGC
GCGGTGCTGGGCGAAATCGCGCTGCTCGACGGAGGCGAGCGCACCGCATCGGTGACGGCGACCAGCGCCGGCAGCGCGCT
GCAACTCGGCCGCAACGCGCTCCGCGACTTTGCCGCGAGCCATCCCGACTTCGCCTGGTCGTTGATGCAGCAGCTCGCGC
GTCGGCTGCGTACCGCCGACCAGACCATCGAAAGCGACCGCGCCTACGCCTCCGGCCCGCGCCTCGCGCGCTATCTGAAA
CGCTTGATCCGCAAGGATGTCGGCGTCGCACAGCGCGTCGAACTCAGCCAGACCGAACTCGGCAATTTCGCCGGAATGAG
CCGCGAACATATCAACCGCCAGCTCAAAAGCTGGGAAGAATCGGGGATCATCGCGCTCGAACAGGGCCGCGTGCGTGTGC
TGGAACCAGAGATACTCGAAGACATAAGCGAAAGCGAAGGATGA

Upstream 100 bases:

>100_bases
ACTCAAATCCAAGCCCTTCGACCCGCATCCCCTGTTTGCAGGATTCATCGAGGCGGCGGTGAAGCAGAGCCGGCTGGTCT
GACCGAAAGGGATTGGGGCG

Downstream 100 bases:

>100_bases
CGGGTTTCGGTCGAATGTCCTCGTTCCCCGTCACCGTGAACTTGTTTCAGGGCCCATGGCCCGACGCTTCCTTCGACGCC
GCAACCGACCAGAGGTCCGG

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MDHARLAELQSPDSIFSGLSVEDWAEITRRAVQVNFAKGKELLVQGDPGDMMLILTEGTARVSMLTAGGREIVLAYAEPG
AVLGEIALLDGGERTASVTATSAGSALQLGRNALRDFAASHPDFAWSLMQQLARRLRTADQTIESDRAYASGPRLARYLK
RLIRKDVGVAQRVELSQTELGNFAGMSREHINRQLKSWEESGIIALEQGRVRVLEPEILEDISESEG

Sequences:

>Translated_227_residues
MDHARLAELQSPDSIFSGLSVEDWAEITRRAVQVNFAKGKELLVQGDPGDMMLILTEGTARVSMLTAGGREIVLAYAEPG
AVLGEIALLDGGERTASVTATSAGSALQLGRNALRDFAASHPDFAWSLMQQLARRLRTADQTIESDRAYASGPRLARYLK
RLIRKDVGVAQRVELSQTELGNFAGMSREHINRQLKSWEESGIIALEQGRVRVLEPEILEDISESEG
>Mature_227_residues
MDHARLAELQSPDSIFSGLSVEDWAEITRRAVQVNFAKGKELLVQGDPGDMMLILTEGTARVSMLTAGGREIVLAYAEPG
AVLGEIALLDGGERTASVTATSAGSALQLGRNALRDFAASHPDFAWSLMQQLARRLRTADQTIESDRAYASGPRLARYLK
RLIRKDVGVAQRVELSQTELGNFAGMSREHINRQLKSWEESGIIALEQGRVRVLEPEILEDISESEG

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=176, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24803; Mature: 24803

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHARLAELQSPDSIFSGLSVEDWAEITRRAVQVNFAKGKELLVQGDPGDMMLILTEGTA
CCCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHEEECCCCEEEEECCCCCEEEEEECCCC
RVSMLTAGGREIVLAYAEPGAVLGEIALLDGGERTASVTATSAGSALQLGRNALRDFAAS
EEEEEECCCCEEEEEECCCCCHHHEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
HPDFAWSLMQQLARRLRTADQTIESDRAYASGPRLARYLKRLIRKDVGVAQRVELSQTEL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
GNFAGMSREHINRQLKSWEESGIIALEQGRVRVLEPEILEDISESEG
HHHCCCCHHHHHHHHHHHHHCCEEEECCCCEEEECHHHHHHHHCCCC
>Mature Secondary Structure
MDHARLAELQSPDSIFSGLSVEDWAEITRRAVQVNFAKGKELLVQGDPGDMMLILTEGTA
CCCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHEEECCCCEEEEECCCCCEEEEEECCCC
RVSMLTAGGREIVLAYAEPGAVLGEIALLDGGERTASVTATSAGSALQLGRNALRDFAAS
EEEEEECCCCEEEEEECCCCCHHHEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
HPDFAWSLMQQLARRLRTADQTIESDRAYASGPRLARYLKRLIRKDVGVAQRVELSQTEL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
GNFAGMSREHINRQLKSWEESGIIALEQGRVRVLEPEILEDISESEG
HHHCCCCHHHHHHHHHHHHHCCEEEECCCCEEEECHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]