BacMap

Definition	Sphingopyxis alaskensis RB2256, complete genome.
Accession	NC_008048
Length	3,345,170

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The map label for this gene is ppi [H]

Identifier: 103486636

GI number: 103486636

Start: 1183856

End: 1184302

Strand: Direct

Name: ppi [H]

Synonym: Sala_1148

Alternate gene names: 103486636

Gene position: 1183856-1184302 (Clockwise)

Preceding gene: 103486633

Following gene: 103486638

Centisome position: 35.39

GC content: 63.76

Gene sequence:

>447_bases
ATGTCCGATACCCTTACGCTTTCGCTGTCGACCGGCGACGTTGTCATCCGTCTGCGCCCCGACCTCGCCCCGTTGCACGT
CGAGCGCATTTCGACGCTCGCACAGCAAGGCTTCTACGACGGTGTCGTCTTTCACCGCGTCATCCCCGGCTTCATGGCGC
AGGGCGGCGACCCTACGGGAACCGGCATGGGCGGCAGCCAGCTTCCCGACCTGCCGCAGGAGTTCAACAGCGAGCCGCAT
GTCCGCGGCGTGTGCTCGATGGCTCGCGCGCAGAACCCGAACAGCGCGAACAGCCAGTTCTTCATCTGCTTCGAGGATGC
CCGCTTCCTCGACAACCAGTATACCGTCTGGGGCGAAGTGATCGAAGGCATGGAACATGTCGATGCGCTGCCCAAGGGCG
AACCGCCGCGCGAGCCGGGCACGATCGTCAAAGCGACGATTTCGTAA

Upstream 100 bases:

>100_bases
CGATGGCAACGCCGCGAACATCTTTTAACGATTTTGGGCGTGGCCTTGGCCGGCCCGCCCCGCTATGGCGCTGCCAACCC
CGATCACTGGAGCCAAGTCT

Downstream 100 bases:

>100_bases
ACTTCGACCCTTCCCCCTCGATGGGGGAAGGATATGAAGCCTTGCCGCATTAGCGGCTAGGCGAAGTTGGATGGGGGTGA
TGGCGCGCCCCTGCGCGGGC

Product: peptidyl-prolyl cis-trans isomerase, cyclophilin type

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSDTLTLSLSTGDVVIRLRPDLAPLHVERISTLAQQGFYDGVVFHRVIPGFMAQGGDPTGTGMGGSQLPDLPQEFNSEPH
VRGVCSMARAQNPNSANSQFFICFEDARFLDNQYTVWGEVIEGMEHVDALPKGEPPREPGTIVKATIS

Sequences:

>Translated_148_residues
MSDTLTLSLSTGDVVIRLRPDLAPLHVERISTLAQQGFYDGVVFHRVIPGFMAQGGDPTGTGMGGSQLPDLPQEFNSEPH
VRGVCSMARAQNPNSANSQFFICFEDARFLDNQYTVWGEVIEGMEHVDALPKGEPPREPGTIVKATIS
>Mature_147_residues
SDTLTLSLSTGDVVIRLRPDLAPLHVERISTLAQQGFYDGVVFHRVIPGFMAQGGDPTGTGMGGSQLPDLPQEFNSEPHV
RGVCSMARAQNPNSANSQFFICFEDARFLDNQYTVWGEVIEGMEHVDALPKGEPPREPGTIVKATIS

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=133, Percent_Identity=41.3533834586466, Blast_Score=100, Evalue=4e-22,
Organism=Homo sapiens, GI24308049, Length=158, Percent_Identity=39.873417721519, Blast_Score=100, Evalue=6e-22,
Organism=Homo sapiens, GI7706339, Length=129, Percent_Identity=36.4341085271318, Blast_Score=89, Evalue=1e-18,
Organism=Homo sapiens, GI64276486, Length=118, Percent_Identity=39.8305084745763, Blast_Score=84, Evalue=6e-17,
Organism=Homo sapiens, GI5031987, Length=128, Percent_Identity=42.96875, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI10863927, Length=130, Percent_Identity=40, Blast_Score=81, Evalue=4e-16,
Organism=Homo sapiens, GI310129267, Length=127, Percent_Identity=39.3700787401575, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI310120620, Length=127, Percent_Identity=39.3700787401575, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI256017253, Length=129, Percent_Identity=38.7596899224806, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI221139811, Length=129, Percent_Identity=38.7596899224806, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI256017251, Length=129, Percent_Identity=38.7596899224806, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI310110104, Length=127, Percent_Identity=38.5826771653543, Blast_Score=77, Evalue=8e-15,
Organism=Homo sapiens, GI22547212, Length=151, Percent_Identity=39.0728476821192, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI7657473, Length=151, Percent_Identity=39.0728476821192, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI310127646, Length=129, Percent_Identity=37.984496124031, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI310114371, Length=129, Percent_Identity=37.984496124031, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI310119221, Length=129, Percent_Identity=37.984496124031, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI239751853, Length=146, Percent_Identity=38.3561643835616, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI113428306, Length=146, Percent_Identity=38.3561643835616, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI169214050, Length=146, Percent_Identity=38.3561643835616, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI178057341, Length=130, Percent_Identity=37.6923076923077, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI22547215, Length=151, Percent_Identity=39.0728476821192, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI169163387, Length=128, Percent_Identity=38.28125, Blast_Score=74, Evalue=4e-14,
Organism=Homo sapiens, GI169164185, Length=128, Percent_Identity=38.28125, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI169163623, Length=128, Percent_Identity=38.28125, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI45439316, Length=132, Percent_Identity=35.6060606060606, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI209417936, Length=128, Percent_Identity=37.5, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI30102944, Length=128, Percent_Identity=37.5, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI219842199, Length=128, Percent_Identity=37.5, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI303305009, Length=123, Percent_Identity=36.5853658536585, Blast_Score=72, Evalue=1e-13,
Organism=Homo sapiens, GI5174637, Length=123, Percent_Identity=36.5853658536585, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI4505991, Length=128, Percent_Identity=39.84375, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI310132444, Length=130, Percent_Identity=37.6923076923077, Blast_Score=66, Evalue=9e-12,
Organism=Homo sapiens, GI310113152, Length=130, Percent_Identity=37.6923076923077, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1786736, Length=137, Percent_Identity=37.2262773722628, Blast_Score=87, Evalue=4e-19,
Organism=Escherichia coli, GI1789763, Length=158, Percent_Identity=34.1772151898734, Blast_Score=87, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17506311, Length=150, Percent_Identity=38.6666666666667, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17539496, Length=127, Percent_Identity=38.5826771653543, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17532641, Length=157, Percent_Identity=36.3057324840764, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71980590, Length=131, Percent_Identity=38.9312977099237, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71980594, Length=131, Percent_Identity=38.9312977099237, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17539498, Length=141, Percent_Identity=39.7163120567376, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71997782, Length=124, Percent_Identity=37.9032258064516, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17566266, Length=134, Percent_Identity=38.0597014925373, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17559074, Length=135, Percent_Identity=37.037037037037, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17552782, Length=133, Percent_Identity=36.8421052631579, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17552780, Length=137, Percent_Identity=36.4963503649635, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17551282, Length=115, Percent_Identity=39.1304347826087, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551284, Length=115, Percent_Identity=39.1304347826087, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17565860, Length=137, Percent_Identity=37.2262773722628, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320359, Length=143, Percent_Identity=41.2587412587413, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321848, Length=130, Percent_Identity=40, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323562, Length=128, Percent_Identity=38.28125, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17986117, Length=154, Percent_Identity=38.3116883116883, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45549139, Length=128, Percent_Identity=42.1875, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24652460, Length=135, Percent_Identity=39.2592592592593, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24664125, Length=128, Percent_Identity=42.1875, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357783, Length=128, Percent_Identity=42.1875, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922376, Length=134, Percent_Identity=35.8208955223881, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17647301, Length=128, Percent_Identity=39.84375, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922912, Length=161, Percent_Identity=34.7826086956522, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357249, Length=123, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24650807, Length=136, Percent_Identity=35.2941176470588, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 16086; Mature: 15955

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLTLSLSTGDVVIRLRPDLAPLHVERISTLAQQGFYDGVVFHRVIPGFMAQGGDPTG
CCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
TGMGGSQLPDLPQEFNSEPHVRGVCSMARAQNPNSANSQFFICFEDARFLDNQYTVWGEV
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHH
IEGMEHVDALPKGEPPREPGTIVKATIS
HHHHHHHHCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
SDTLTLSLSTGDVVIRLRPDLAPLHVERISTLAQQGFYDGVVFHRVIPGFMAQGGDPTG
CCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
TGMGGSQLPDLPQEFNSEPHVRGVCSMARAQNPNSANSQFFICFEDARFLDNQYTVWGEV
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHH
IEGMEHVDALPKGEPPREPGTIVKATIS
HHHHHHHHCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA