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The map label for this gene is gyrB [H]

Identifier: 103486580

GI number: 103486580

Start: 1119311

End: 1121290

Strand: Reverse

Name: gyrB [H]

Synonym: Sala_1091

Alternate gene names: 103486580

Gene position: 1121290-1119311 (Counterclockwise)

Preceding gene: 103486582

Following gene: 103486579

Centisome position: 33.52

GC content: 65.4

Gene sequence:

>1980_bases
ATGAGTCACGATTTGTTCGACGCCGCGATCCCCGCTTCCGACAGCTATAATGCGTCGCAGATCGAGGTGCTGGAGGGGCT
CGAACCCGTCCGCCGCCGCCCCGGCATGTATATCGGAGGGACCGACGAGCGCGCGCTGCACCATCTCGCCGCCGAGGTGA
TCGACAACAGCATGGACGAGGCGGTCGCGGGTCATGCGACGCGCATCGAGGTTACGCTCGACGTCGGCAACCGGCTCACG
ATCGTCGACAACGGGCGCGGGATGCCGGTCGATCCGCATCCGAAGTTTCCGGGCAAGTCGGCGCTCGAGGTCATCATGAC
CATGCTCCATTCGGGCGGCAAGTTCGAGGGCAAGGCCTATGCAACCTCGGGCGGCCTTCACGGTGTCGGAGTGAGCGTCG
TCAACGCGCTGTCGGTCGAGACCGAGATCGAGGTCGCGCGCGCCAAACAGCTTTATCGTCAGCGCTTCGCGCGCGGGGCG
CCGCTCGGTCCGCTCGAAGAATTGGGGGCCACGCCGAACCGGCGCGGCACCAGCGTTTCCTTTATCCCCGACCCCGAAAT
CTTCGGCGAGCATGGCCGCTTCAAGCCGCAGCGGCTTTACCGCTTGGCACGCTCGAAGGCCTATCTCTTCGCCGGCGTGG
AAATACGCTGGAAATGCGCACCCGCGCTGATCGGCGACGACACGCCCAGCGAGGCGGTTTTCCAGTTCCCCGGCGGCTTG
GCCGACCATCTCAAGGAGCAGATCGGCGCCCGCGAATGCGCGACTGCCGATCCCTTTGTCGGGCGACAGGATTTTCCGGG
CGATCAGGGCCGCGCCGAATGGGCGATCGCCTGGCCACTGTGGTCCGATGGTTCCTACAGCTGGTATTGCAACACCATCC
CCACCCCCGCGGGCGGGACGCACGAGGCCGGGCTGCGCGCGGCGCTGACCAAGGGCCTGCGCGCGTTCGGCGAGCTGATC
GGCCAGAAAAAGGCGGCGCAGATCACCGCCGAGGACGTTTTCAACGGCAGCGAGATGATGCTCTCGGTCTTCATCCGCGA
CCCCCAGTTCCAGAGCCAGACCAAGGACCGGCTGTCCAGCCCCGAGGCCGCGCGCCTGACCGAAAATGCCGTGCGCGATC
ATTTCGACCATTTCCTGTCGGACAATATGGACCGCGGCCGCGCGCTGCTGGGCCTCGTTCTCGACCGGATGGACGAGCGG
CTGAAGCGCAAGGCCGAGCGCGAGGTCAAGCGCAAGACCGCGACGAGCGGACGCAAGCTCCGCCTGCCCGGCAAGCTCAC
CGATTGCGCGAACGACGGCCCCGAAGGCACCGAGCTTTTCATCGTCGAAGGCGACAGCGCGGGCGGCAGCGCCAAGCAGG
CACGCGACCGCAAGACGCAGGCGATCCTACCGATCCGCGGCAAGATATTGAACGTCGCGAGCGCCAGCGCCGACAAGATC
CGCGCCAATCAGGAGATCGCCGACCTGGCGCTCGCGCTCGGTTGCGGGATGCGCAAGGATTGCGATGCCGACCGGCTAAG
ATACGAAAAAATTGTCATTATGACCGACGCCGATGTCGACGGCGCGCATATCGCGACCTTGCTGATGACCTTCTTCTTTC
AGGAGATGCCCGACATCGTCCGGAACGGGCATGTCTATCTCGCGCAGCCGCCGCTTTACCGTCTGACCGCGGGCGGCCTC
TCGGCCTATGCGCGCGACGACGCGCACCGCGCCGAGCTGGAGGCAACGCTGTTCAAGGGCAAGAAGGTCGAGGTCGGCCG
GTTCAAGGGGCTGGGCGAAATGAACCCGAACCAGCTCAAGGAAACGACGATGGACCCCGCCACGCGCAGCCTGCTGCGCG
TCACGCTGCCGCAGGAATATGAAGAGCGTCATCTGGTCAAGGACCTTGTCGACCGGCTGATGGGCAGGAACCCTGAACAT
CGTTTCCAGTTCATCCAGGCCAATGCCGCCAGCCTCGACGAGGCCGCGATCGACGCCTAG

Upstream 100 bases:

>100_bases
GCGCAGGCCGGAATGCGGTGAATCGATGCCGCGATGCGCCGGATAACGACCGTTAAAACCGCGCTTGCCAGTGCCCTTCC
CCGACCCGTATAGCCATGCC

Downstream 100 bases:

>100_bases
CGCCGATGATTCCGCCGTGGCGGAAGGCGCGCGAAATGGGGAGAATGATACCATGATCCGCCGCCGCATCGCCCGCTCAT
TCGTTGCCGTTCTGCTCACC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MSHDLFDAAIPASDSYNASQIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVIDNSMDEAVAGHATRIEVTLDVGNRLT
IVDNGRGMPVDPHPKFPGKSALEVIMTMLHSGGKFEGKAYATSGGLHGVGVSVVNALSVETEIEVARAKQLYRQRFARGA
PLGPLEELGATPNRRGTSVSFIPDPEIFGEHGRFKPQRLYRLARSKAYLFAGVEIRWKCAPALIGDDTPSEAVFQFPGGL
ADHLKEQIGARECATADPFVGRQDFPGDQGRAEWAIAWPLWSDGSYSWYCNTIPTPAGGTHEAGLRAALTKGLRAFGELI
GQKKAAQITAEDVFNGSEMMLSVFIRDPQFQSQTKDRLSSPEAARLTENAVRDHFDHFLSDNMDRGRALLGLVLDRMDER
LKRKAEREVKRKTATSGRKLRLPGKLTDCANDGPEGTELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASASADKI
RANQEIADLALALGCGMRKDCDADRLRYEKIVIMTDADVDGAHIATLLMTFFFQEMPDIVRNGHVYLAQPPLYRLTAGGL
SAYARDDAHRAELEATLFKGKKVEVGRFKGLGEMNPNQLKETTMDPATRSLLRVTLPQEYEERHLVKDLVDRLMGRNPEH
RFQFIQANAASLDEAAIDA

Sequences:

>Translated_659_residues
MSHDLFDAAIPASDSYNASQIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVIDNSMDEAVAGHATRIEVTLDVGNRLT
IVDNGRGMPVDPHPKFPGKSALEVIMTMLHSGGKFEGKAYATSGGLHGVGVSVVNALSVETEIEVARAKQLYRQRFARGA
PLGPLEELGATPNRRGTSVSFIPDPEIFGEHGRFKPQRLYRLARSKAYLFAGVEIRWKCAPALIGDDTPSEAVFQFPGGL
ADHLKEQIGARECATADPFVGRQDFPGDQGRAEWAIAWPLWSDGSYSWYCNTIPTPAGGTHEAGLRAALTKGLRAFGELI
GQKKAAQITAEDVFNGSEMMLSVFIRDPQFQSQTKDRLSSPEAARLTENAVRDHFDHFLSDNMDRGRALLGLVLDRMDER
LKRKAEREVKRKTATSGRKLRLPGKLTDCANDGPEGTELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASASADKI
RANQEIADLALALGCGMRKDCDADRLRYEKIVIMTDADVDGAHIATLLMTFFFQEMPDIVRNGHVYLAQPPLYRLTAGGL
SAYARDDAHRAELEATLFKGKKVEVGRFKGLGEMNPNQLKETTMDPATRSLLRVTLPQEYEERHLVKDLVDRLMGRNPEH
RFQFIQANAASLDEAAIDA
>Mature_658_residues
SHDLFDAAIPASDSYNASQIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVIDNSMDEAVAGHATRIEVTLDVGNRLTI
VDNGRGMPVDPHPKFPGKSALEVIMTMLHSGGKFEGKAYATSGGLHGVGVSVVNALSVETEIEVARAKQLYRQRFARGAP
LGPLEELGATPNRRGTSVSFIPDPEIFGEHGRFKPQRLYRLARSKAYLFAGVEIRWKCAPALIGDDTPSEAVFQFPGGLA
DHLKEQIGARECATADPFVGRQDFPGDQGRAEWAIAWPLWSDGSYSWYCNTIPTPAGGTHEAGLRAALTKGLRAFGELIG
QKKAAQITAEDVFNGSEMMLSVFIRDPQFQSQTKDRLSSPEAARLTENAVRDHFDHFLSDNMDRGRALLGLVLDRMDERL
KRKAEREVKRKTATSGRKLRLPGKLTDCANDGPEGTELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASASADKIR
ANQEIADLALALGCGMRKDCDADRLRYEKIVIMTDADVDGAHIATLLMTFFFQEMPDIVRNGHVYLAQPPLYRLTAGGLS
AYARDDAHRAELEATLFKGKKVEVGRFKGLGEMNPNQLKETTMDPATRSLLRVTLPQEYEERHLVKDLVDRLMGRNPEHR
FQFIQANAASLDEAAIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=632, Percent_Identity=25, Blast_Score=124, Evalue=4e-28,
Organism=Homo sapiens, GI19913408, Length=643, Percent_Identity=24.4167962674961, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI1789408, Length=639, Percent_Identity=44.2879499217527, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI48994957, Length=562, Percent_Identity=43.7722419928826, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI212645845, Length=563, Percent_Identity=22.3801065719361, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17535065, Length=587, Percent_Identity=22.9982964224872, Blast_Score=105, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324241, Length=635, Percent_Identity=24.5669291338583, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI17136538, Length=592, Percent_Identity=23.1418918918919, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72380; Mature: 72249

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00626 RCC1_2 ; PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDLFDAAIPASDSYNASQIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVIDNSMDE
CCCCCCHHCCCCCCCCCCHHHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHCHHHH
AVAGHATRIEVTLDVGNRLTIVDNGRGMPVDPHPKFPGKSALEVIMTMLHSGGKFEGKAY
HHCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
ATSGGLHGVGVSVVNALSVETEIEVARAKQLYRQRFARGAPLGPLEELGATPNRRGTSVS
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEE
FIPDPEIFGEHGRFKPQRLYRLARSKAYLFAGVEIRWKCAPALIGDDTPSEAVFQFPGGL
ECCCHHHHCCCCCCCHHHHHHHHHCCEEEEECEEEEEEECCCCCCCCCCCHHHHHCCCCH
ADHLKEQIGARECATADPFVGRQDFPGDQGRAEWAIAWPLWSDGSYSWYCNTIPTPAGGT
HHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCCCCCC
HEAGLRAALTKGLRAFGELIGQKKAAQITAEDVFNGSEMMLSVFIRDPQFQSQTKDRLSS
HHHHHHHHHHHHHHHHHHHHCCHHHHEEEHHHHCCCHHHEEEEEECCCCHHHHHHHHHCC
PEAARLTENAVRDHFDHFLSDNMDRGRALLGLVLDRMDERLKRKAEREVKRKTATSGRKL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RLPGKLTDCANDGPEGTELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASASADKI
ECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCEECCCCCEEEEHHCCCHHHH
RANQEIADLALALGCGMRKDCDADRLRYEKIVIMTDADVDGAHIATLLMTFFFQEMPDIV
HCCHHHHHHHHHHCCCCCCCCCHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RNGHVYLAQPPLYRLTAGGLSAYARDDAHRAELEATLFKGKKVEVGRFKGLGEMNPNQLK
HCCCEEEECCCCEEEECCCHHHHHCCCHHHHHHHHHHHCCCEEECHHCCCCCCCCHHHHH
ETTMDPATRSLLRVTLPQEYEERHLVKDLVDRLMGRNPEHRFQFIQANAASLDEAAIDA
HHHCCHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCHHHCCC
>Mature Secondary Structure 
SHDLFDAAIPASDSYNASQIEVLEGLEPVRRRPGMYIGGTDERALHHLAAEVIDNSMDE
CCCCCHHCCCCCCCCCCHHHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHCHHHH
AVAGHATRIEVTLDVGNRLTIVDNGRGMPVDPHPKFPGKSALEVIMTMLHSGGKFEGKAY
HHCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
ATSGGLHGVGVSVVNALSVETEIEVARAKQLYRQRFARGAPLGPLEELGATPNRRGTSVS
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEE
FIPDPEIFGEHGRFKPQRLYRLARSKAYLFAGVEIRWKCAPALIGDDTPSEAVFQFPGGL
ECCCHHHHCCCCCCCHHHHHHHHHCCEEEEECEEEEEEECCCCCCCCCCCHHHHHCCCCH
ADHLKEQIGARECATADPFVGRQDFPGDQGRAEWAIAWPLWSDGSYSWYCNTIPTPAGGT
HHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCCCCCC
HEAGLRAALTKGLRAFGELIGQKKAAQITAEDVFNGSEMMLSVFIRDPQFQSQTKDRLSS
HHHHHHHHHHHHHHHHHHHHCCHHHHEEEHHHHCCCHHHEEEEEECCCCHHHHHHHHHCC
PEAARLTENAVRDHFDHFLSDNMDRGRALLGLVLDRMDERLKRKAEREVKRKTATSGRKL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RLPGKLTDCANDGPEGTELFIVEGDSAGGSAKQARDRKTQAILPIRGKILNVASASADKI
ECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCEECCCCCEEEEHHCCCHHHH
RANQEIADLALALGCGMRKDCDADRLRYEKIVIMTDADVDGAHIATLLMTFFFQEMPDIV
HCCHHHHHHHHHHCCCCCCCCCHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RNGHVYLAQPPLYRLTAGGLSAYARDDAHRAELEATLFKGKKVEVGRFKGLGEMNPNQLK
HCCCEEEECCCCEEEECCCHHHHHCCCHHHHHHHHHHHCCCEEECHHCCCCCCCCHHHHH
ETTMDPATRSLLRVTLPQEYEERHLVKDLVDRLMGRNPEHRFQFIQANAASLDEAAIDA
HHHCCHHHHHHEEECCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]