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The map label for this gene is pyrF

Identifier: 103486555

GI number: 103486555

Start: 1099191

End: 1099865

Strand: Reverse

Name: pyrF

Synonym: Sala_1066

Alternate gene names: 103486555

Gene position: 1099865-1099191 (Counterclockwise)

Preceding gene: 103486556

Following gene: 103486554

Centisome position: 32.88

GC content: 66.52

Gene sequence:

>675_bases
ATGACTTCGCCCATCTATCTCGCCATCGACACCCCGCATCTCGACGCCGCTCTGACGCTGGCGCAAAAGGTGCGGCATCA
TGTCGGCGGGCTGAAGCTGGGGCTCGAGTTTTTCTGCGCCAACGGCCATCATGGCGTCCATGAAATGGCGAAGCTCGGCC
TGCCGATCTTCCTCGACCTGAAGCTGCACGACATTCCGAATACGGTGGCAAAGGCGATCCAGGCGCTGCGCCCGCTCGAA
CCCGCGATCCTGACGGTTCACGCCGCGGGCGGCCGCGCGATGCTCGAAGAAGCGAAAGCGGTTGCGGGCAAGGATACCAG
AGTGATCGCCGTCACCGTGCTCACCAGCCTCGACGCGCCCGATCTTGAAGACATCGGCCTCACCGGCACGCCGCACGATC
AGGTCGTACGCCTTGCCGCGCTGGCGCGTGAGGCCGGGCTCGACGGCATTGTCTGTTCGGGTCAGGAAGTGAAATCGGCG
CGCAAGGCGTGGCCCGGCGGCTTCTTCGTCGTCCCCGGCGTGCGCCCCGCAAACGGCCGGATCGGCGACCAGAAACGCAT
CGTCACGCCCGCGCAGGCGATGTCCGACGGCGCCTCGATCCTCGTCGTCGGCCGCCCGATCACCCAGTCGTCCGACCCCG
ACCTTGCGGCGCGCGAGATTGAAGCGACGCTCTGA

Upstream 100 bases:

>100_bases
TCGCGCACCGCCCCGTCGAACCGGCGCCCGCGCCCTCGGCCACGGCACCGGTCAACGACATTCCCCCTGCCCCCACCGAC
CTTTTCGCCCCGGACAAGCC

Downstream 100 bases:

>100_bases
TTCATTTCCGTTCGTTTCCATAGCCATCGTCATTGCGAGGAGCCAAAGGCGACGAAGCAATCTCCAGCTATCGAGATTGC
GCTACGCTAATAGCTGGAGA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MTSPIYLAIDTPHLDAALTLAQKVRHHVGGLKLGLEFFCANGHHGVHEMAKLGLPIFLDLKLHDIPNTVAKAIQALRPLE
PAILTVHAAGGRAMLEEAKAVAGKDTRVIAVTVLTSLDAPDLEDIGLTGTPHDQVVRLAALAREAGLDGIVCSGQEVKSA
RKAWPGGFFVVPGVRPANGRIGDQKRIVTPAQAMSDGASILVVGRPITQSSDPDLAAREIEATL

Sequences:

>Translated_224_residues
MTSPIYLAIDTPHLDAALTLAQKVRHHVGGLKLGLEFFCANGHHGVHEMAKLGLPIFLDLKLHDIPNTVAKAIQALRPLE
PAILTVHAAGGRAMLEEAKAVAGKDTRVIAVTVLTSLDAPDLEDIGLTGTPHDQVVRLAALAREAGLDGIVCSGQEVKSA
RKAWPGGFFVVPGVRPANGRIGDQKRIVTPAQAMSDGASILVVGRPITQSSDPDLAAREIEATL
>Mature_223_residues
TSPIYLAIDTPHLDAALTLAQKVRHHVGGLKLGLEFFCANGHHGVHEMAKLGLPIFLDLKLHDIPNTVAKAIQALRPLEP
AILTVHAAGGRAMLEEAKAVAGKDTRVIAVTVLTSLDAPDLEDIGLTGTPHDQVVRLAALAREAGLDGIVCSGQEVKSAR
KAWPGGFFVVPGVRPANGRIGDQKRIVTPAQAMSDGASILVVGRPITQSSDPDLAAREIEATL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=45.1754385964912, Blast_Score=175, Evalue=2e-45,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_SPHAL (Q1GU89)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616116.1
- ProteinModelPortal:   Q1GU89
- GeneID:   4082331
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1066
- NMPDR:   fig|317655.9.peg.1005
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK835196
- BioCyc:   SALA317655:SALA_1066-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 23549; Mature: 23417

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 115-115 BINDING 175-175 BINDING 184-184 BINDING 204-204 BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPIYLAIDTPHLDAALTLAQKVRHHVGGLKLGLEFFCANGHHGVHEMAKLGLPIFLDL
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEECEEEEECCCCCCHHHHHHCCCCEEEEE
KLHDIPNTVAKAIQALRPLEPAILTVHAAGGRAMLEEAKAVAGKDTRVIAVTVLTSLDAP
EECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCC
DLEDIGLTGTPHDQVVRLAALAREAGLDGIVCSGQEVKSARKAWPGGFFVVPGVRPANGR
CHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCEEEECCCCCCCCC
IGDQKRIVTPAQAMSDGASILVVGRPITQSSDPDLAAREIEATL
CCCCCEEECHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TSPIYLAIDTPHLDAALTLAQKVRHHVGGLKLGLEFFCANGHHGVHEMAKLGLPIFLDL
CCCEEEEEECCCCHHHHHHHHHHHHHHCCEEECEEEEECCCCCCHHHHHHCCCCEEEEE
KLHDIPNTVAKAIQALRPLEPAILTVHAAGGRAMLEEAKAVAGKDTRVIAVTVLTSLDAP
EECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCC
DLEDIGLTGTPHDQVVRLAALAREAGLDGIVCSGQEVKSARKAWPGGFFVVPGVRPANGR
CHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCEEEECCCCCCCCC
IGDQKRIVTPAQAMSDGASILVVGRPITQSSDPDLAAREIEATL
CCCCCEEECHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA