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The map label for this gene is mscL

Identifier: 103486481

GI number: 103486481

Start: 1017357

End: 1017779

Strand: Direct

Name: mscL

Synonym: Sala_0992

Alternate gene names: 103486481

Gene position: 1017357-1017779 (Clockwise)

Preceding gene: 103486476

Following gene: 103486483

Centisome position: 30.41

GC content: 62.65

Gene sequence:

>423_bases
ATGTTGGGCGAGTTCAGGCAGTTCATCGCCAGGGGCAATGTCATGGACCTGGCGGTCGGCGTCATCATCGGCGGCGCCTT
TGCGACCATCACCGGTTCGCTGACCGAAGATGTCATCATGCCGCTGGTCGGCGCGCTCTTTGGCGGGGTCGATTTTTCGA
ACCGGTTCATCCTGCTCGGCAGCGTGCCCGACGGGATGTCGGCGACCGACTATGCCGCGCTCAAGGAGGCCGGGGTCGCG
ATGATCGGTTACGGCGCCTTCGTCACGGCGGTCATCAATTTCCTGATCCTGGCCTTCATCATCTTCCTGCTCGTGCGCTG
GGTGAACAAGGTCGTCCGCAAGCCGGAGGCGGACGCCTCGCCCGCGGGGCCGAGCGAGGTCGATCTGCTCACCGAAATCC
GCGACGAATTGCGAAGAAAATAA

Upstream 100 bases:

>100_bases
CGTTTTGCCGCAACAATACACATAATAGGAAGCCGCTTGCCTTTGTGCAATCGCCACGGCACCCTTTGCGGACAGGACCG
GATTTTCAGGGAGCGTCAAT

Downstream 100 bases:

>100_bases
GGGAGTTGAGCCCCTTGCGGGACGAATGACCCGTCTGCGCCCTTTCCAAGCGCCGGCGGGTTTCCTATATGGGGGGTGTC
GGCTTCGGCCGACTATGAGG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MLGEFRQFIARGNVMDLAVGVIIGGAFATITGSLTEDVIMPLVGALFGGVDFSNRFILLGSVPDGMSATDYAALKEAGVA
MIGYGAFVTAVINFLILAFIIFLLVRWVNKVVRKPEADASPAGPSEVDLLTEIRDELRRK

Sequences:

>Translated_140_residues
MLGEFRQFIARGNVMDLAVGVIIGGAFATITGSLTEDVIMPLVGALFGGVDFSNRFILLGSVPDGMSATDYAALKEAGVA
MIGYGAFVTAVINFLILAFIIFLLVRWVNKVVRKPEADASPAGPSEVDLLTEIRDELRRK
>Mature_140_residues
MLGEFRQFIARGNVMDLAVGVIIGGAFATITGSLTEDVIMPLVGALFGGVDFSNRFILLGSVPDGMSATDYAALKEAGVA
MIGYGAFVTAVINFLILAFIIFLLVRWVNKVVRKPEADASPAGPSEVDLLTEIRDELRRK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=141, Percent_Identity=43.9716312056738, Blast_Score=107, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_SPHAL (Q1GUG3)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616042.1
- ProteinModelPortal:   Q1GUG3
- SMR:   Q1GUG3
- GeneID:   4081680
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0992
- NMPDR:   fig|317655.9.peg.931
- HOGENOM:   HBG727604
- OMA:   AIVTAFT
- ProtClustDB:   PRK13952
- BioCyc:   SALA317655:SALA_0992-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14980; Mature: 14980

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10f47d84)-; HASH(0x114f50fc)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGEFRQFIARGNVMDLAVGVIIGGAFATITGSLTEDVIMPLVGALFGGVDFSNRFILLG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
SVPDGMSATDYAALKEAGVAMIGYGAFVTAVINFLILAFIIFLLVRWVNKVVRKPEADAS
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAGPSEVDLLTEIRDELRRK
CCCCHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MLGEFRQFIARGNVMDLAVGVIIGGAFATITGSLTEDVIMPLVGALFGGVDFSNRFILLG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
SVPDGMSATDYAALKEAGVAMIGYGAFVTAVINFLILAFIIFLLVRWVNKVVRKPEADAS
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAGPSEVDLLTEIRDELRRK
CCCCHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA