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The map label for this gene is pntAA [H]

Identifier: 103486460

GI number: 103486460

Start: 996335

End: 997456

Strand: Direct

Name: pntAA [H]

Synonym: Sala_0971

Alternate gene names: 103486460

Gene position: 996335-997456 (Clockwise)

Preceding gene: 103486456

Following gene: 103486461

Centisome position: 29.78

GC content: 66.76

Gene sequence:

>1122_bases
ATGCGCATCGCCGTATTGAAGGAGCTCGCCGCTGGCGAGACCCGGGTCGCCGCGACCCCGGAAACCGTGAAGAAATTCAT
TGGGCTGGGCGCCGAAGTTGCCGTCGAATCGGGGGCGGGGGAGCAGGCCTCGATTGCCGACGCCGATTATGGCGCCGCGG
GCGCGAGCGTTGGCAGCCGCGCCGAGGTGCTGAAGGGCGCGAACATCATTCTCGGCATCCAGGGGCCGGACCCGGCGACG
CTGTCGGGCTTCGCCGAAGGCGCGTGGCTGGCGGCCGGGCTCAACCCCTTCGGCGCGCGCGCGCGTGTCGACGAATATGC
GAAGCTGGGGCTGGAGGCGCTGGCGATGGAGTTCATGCCGCGCATCACGCGCGCGCAGTCGATGGACATCCTTTCCAGCC
AGGCGAATCTTGCCGGTTACAAGGCGGTGCTGATGGCGGCGAATGCCTATGGTCGCGCCTTTCCGATGATGATGACCGCC
GCGGGCACCGTCAGCGCGGCCAAGGCGTTCATCATGGGGGTCGGCGTCGCCGGATTGCAGGCGATCGCCACTGCGCGGCG
GCTCGGCGCGCAGGTCAGTGCGACCGACGTGCGCTCGGCGACGAAGGAGCAGATTCTCTCGCTGGGCGCCAAGCCGATCT
TCGTCGAAAATGTCGCGGGGATCGAGGGCGAGGGCGCGGGCGGTTATGCCACCGAAATGTCCGACGAATATAAGGCGGCG
CAGGCCGAACTCGTGTCAGCGCATATCGCCAAGCAGGACATCGTCATCACCACCGCGCTGATTCCGGGGCGCCCCGCGCC
GCGGCTGATTTCGGACGCACAGCTTGCGACGATGCGATCGGGCAGCGTGATCGTCGACATGGCGGCCGAAAGCGGCGGCA
ATGTCGAAGGCTCGGTCTCGGGCGAAACGAGGCGCATTCACGGCGTCACCGTGATCGGCGCGAAAAATATCGCCGCGCTG
ATGCCCGCCGACACCAGCGCATTGTTCAGCCGTAACCTGTTCAACTTCCTTTCCGCCTTCTGGGACAAGGAGCAGGGCAA
GCCCGTGCTCGACGAGGAAATCGGCAACGCCGTGCGGCTGACGCAGGGCGGCAAGGTCGTCAACGAGCGGCTGCTGGGGT
AA

Upstream 100 bases:

>100_bases
ACCTATTCGGGTTTCCTCAGCCTGCTGAAGGTCGGGGCGATCCTGACCGGGATCGCGACGTTTATCGTCGTTCTCCTGAT
CGCGTCCTGACCTGTCTGTC

Downstream 100 bases:

>100_bases
TCGGATATGGGCCTGTTCAGCGCCGGGGACCTCGACGTTTCGCGCGCGCAGGCCGAGTTTCAGCCATGGCTGATCGACGG
CGAACAGGTGCTCGCGGCGT

Product: alanine dehydrogenase/PNT-like protein

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRIAVLKELAAGETRVAATPETVKKFIGLGAEVAVESGAGEQASIADADYGAAGASVGSRAEVLKGANIILGIQGPDPAT
LSGFAEGAWLAAGLNPFGARARVDEYAKLGLEALAMEFMPRITRAQSMDILSSQANLAGYKAVLMAANAYGRAFPMMMTA
AGTVSAAKAFIMGVGVAGLQAIATARRLGAQVSATDVRSATKEQILSLGAKPIFVENVAGIEGEGAGGYATEMSDEYKAA
QAELVSAHIAKQDIVITTALIPGRPAPRLISDAQLATMRSGSVIVDMAAESGGNVEGSVSGETRRIHGVTVIGAKNIAAL
MPADTSALFSRNLFNFLSAFWDKEQGKPVLDEEIGNAVRLTQGGKVVNERLLG

Sequences:

>Translated_373_residues
MRIAVLKELAAGETRVAATPETVKKFIGLGAEVAVESGAGEQASIADADYGAAGASVGSRAEVLKGANIILGIQGPDPAT
LSGFAEGAWLAAGLNPFGARARVDEYAKLGLEALAMEFMPRITRAQSMDILSSQANLAGYKAVLMAANAYGRAFPMMMTA
AGTVSAAKAFIMGVGVAGLQAIATARRLGAQVSATDVRSATKEQILSLGAKPIFVENVAGIEGEGAGGYATEMSDEYKAA
QAELVSAHIAKQDIVITTALIPGRPAPRLISDAQLATMRSGSVIVDMAAESGGNVEGSVSGETRRIHGVTVIGAKNIAAL
MPADTSALFSRNLFNFLSAFWDKEQGKPVLDEEIGNAVRLTQGGKVVNERLLG
>Mature_373_residues
MRIAVLKELAAGETRVAATPETVKKFIGLGAEVAVESGAGEQASIADADYGAAGASVGSRAEVLKGANIILGIQGPDPAT
LSGFAEGAWLAAGLNPFGARARVDEYAKLGLEALAMEFMPRITRAQSMDILSSQANLAGYKAVLMAANAYGRAFPMMMTA
AGTVSAAKAFIMGVGVAGLQAIATARRLGAQVSATDVRSATKEQILSLGAKPIFVENVAGIEGEGAGGYATEMSDEYKAA
QAELVSAHIAKQDIVITTALIPGRPAPRLISDAQLATMRSGSVIVDMAAESGGNVEGSVSGETRRIHGVTVIGAKNIAAL
MPADTSALFSRNLFNFLSAFWDKEQGKPVLDEEIGNAVRLTQGGKVVNERLLG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=352, Percent_Identity=39.4886363636364, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI122939153, Length=352, Percent_Identity=39.4886363636364, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1787887, Length=352, Percent_Identity=42.3295454545455, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17550456, Length=337, Percent_Identity=43.026706231454, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 38366; Mature: 38366

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVLKELAAGETRVAATPETVKKFIGLGAEVAVESGAGEQASIADADYGAAGASVGSR
CCHHHHHHHHCCCCEEECCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCH
AEVLKGANIILGIQGPDPATLSGFAEGAWLAAGLNPFGARARVDEYAKLGLEALAMEFMP
HHHHCCCEEEEEECCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
RITRAQSMDILSSQANLAGYKAVLMAANAYGRAFPMMMTAAGTVSAAKAFIMGVGVAGLQ
HHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHH
AIATARRLGAQVSATDVRSATKEQILSLGAKPIFVENVAGIEGEGAGGYATEMSDEYKAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHCCCHHHHHH
QAELVSAHIAKQDIVITTALIPGRPAPRLISDAQLATMRSGSVIVDMAAESGGNVEGSVS
HHHHHHHHHHHCCEEEEEEECCCCCCCCHHCCCHHHEECCCCEEEEEECCCCCCCEEECC
GETRRIHGVTVIGAKNIAALMPADTSALFSRNLFNFLSAFWDKEQGKPVLDEEIGNAVRL
CCCEEEEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEE
TQGGKVVNERLLG
ECCCHHHHHHCCC
>Mature Secondary Structure
MRIAVLKELAAGETRVAATPETVKKFIGLGAEVAVESGAGEQASIADADYGAAGASVGSR
CCHHHHHHHHCCCCEEECCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCH
AEVLKGANIILGIQGPDPATLSGFAEGAWLAAGLNPFGARARVDEYAKLGLEALAMEFMP
HHHHCCCEEEEEECCCCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
RITRAQSMDILSSQANLAGYKAVLMAANAYGRAFPMMMTAAGTVSAAKAFIMGVGVAGLQ
HHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHH
AIATARRLGAQVSATDVRSATKEQILSLGAKPIFVENVAGIEGEGAGGYATEMSDEYKAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHCCCHHHHHH
QAELVSAHIAKQDIVITTALIPGRPAPRLISDAQLATMRSGSVIVDMAAESGGNVEGSVS
HHHHHHHHHHHCCEEEEEEECCCCCCCCHHCCCHHHEECCCCEEEEEECCCCCCCEEECC
GETRRIHGVTVIGAKNIAALMPADTSALFSRNLFNFLSAFWDKEQGKPVLDEEIGNAVRL
CCCEEEEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEE
TQGGKVVNERLLG
ECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]