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The map label for this gene is lcfB [H]

Identifier: 103486402

GI number: 103486402

Start: 921799

End: 923346

Strand: Direct

Name: lcfB [H]

Synonym: Sala_0912

Alternate gene names: 103486402

Gene position: 921799-923346 (Clockwise)

Preceding gene: 103486396

Following gene: 103486403

Centisome position: 27.56

GC content: 60.92

Gene sequence:

>1548_bases
ATGCATCCGCGCCATTTTGCGCGAAAAGCGCCCGATCGTCCAGCCATCGTCATGGCGGCAAGCGGAGAAACGGTCAGCTA
TTCCGCGCTCGAAAATGTGGCGAATCGTGGAGCACAGCTTTTTCGCTCACTGGGGATAGCGACGGGTGACACGATCGCGC
TTTGGCTCAAAAACTGCCGCGATTATTTCGAAATCTATTGGGCGGCGCAGCGGGCCGGACTTTATGTCTGCCCGATCGCG
ACGCATCTGACCGTAGAGGAAGCGGCCTATATATTGAACGACAGCGGCGCGTGCCTTCTGATCACCCATGCCGATGTAGC
GGCTGCGCCGGACCTTGTCAGCGAACGTCAGCGCCTGATCCCGGACGTGCGTCACATATTCGGCCTGGAGGCGGGAATGG
ACGGATTTCCGTCATGGCGCGCGGCGCTGACGGGCTTTCCGTCCAAACCGATCGCGGACGAAACCGCTGGTTATCATCTG
GTTTATTCGTCCGGCACTACCGGACGGCCCAAGGGCATAAGGCTGCCGCTCAGCGGCGAGGCGGCCGATGCGCCGCACAT
GCTGGGCGATCGGGTCGCCGGGCGCTATGGCGTCGGGGAGGATAGCGTCATCCTGTCGCCCGCACCGCTCTATCATACCG
CGCCCTTGGTCTATGGCATGGTTGCGCATCGGCTGGGCGCAACATTGATCGTCATGGACCATTTCGATGCCGAGGACATG
TTGCGCCTGATCGAACGCCATCGCGTCACCTTTATCCAGATGGTGCCGACGATGTTCGTGCGCCTGCTTGCGCTGCCGGG
TACGACACGGGCGCAATATGATCTCTCGTCGCTGCAAAAGATCGTTCATGCCGCAGCGCCTTGTCCGGTCGAGATCAAGC
GCCGGATGATCGACTGGCTCGGCCCCATCATCTATGAATATTATGGCGGGTCGGAAGGCAATGGATCGACCTTTATCACG
CCGCAGGAATGGCTGGAGCATCCCGGATCGGTCGGGCGCGCCGACTGGGGAACGCTGCATATTTGCGACGAGGAGGGGAA
TGAGGTGCCACCGGGCGTCGACGGGCTGATCTATTTTGAAGGCGGCTGGGATTTCCAGTATCTGAACGACCCCGAAAAGA
CGCGTGACGCGCGACATCCGCGACATCCGGCATGGAGCACACTGGGCGACATCGGCCATGTCGATAAGGATGGTTATCTT
TACCTGACCGACCGCAAGGGTTTCATGATCATATCGGGCGGCGTGAACATCTATCCGCAGGAAGTCGAAAATCTGCTGAT
CACTCACCCGCGCGTCGCCGACGCGGCCGTTATCGGCGTGCCGAGCGCCGAGTTCGGCGAAGCGGTGAAAGCGGTCGTGC
AACCGCTCGATCCCGCCGACGCGACCGATGAATTTGCGGAAGAGTTGCTGCGTTTCTGCCGCGCCCGGCTGAGCCATGTG
AAATGCCCGCGATCGATCGATTTCGACCCTGCGCTGCCCCGGCTCGACACGGGCAAGCTTTACAAGCGGTCGATCCGCGA
CCGCTATTGGCCTGTCGCGCCGACATAG

Upstream 100 bases:

>100_bases
CCCATTGCCGCTCCGTTCGGATTCGTCGTCGGCCGCAATATGATAATCGACACAGAAAGTAAACGTAGTTATATAACTGA
AAGGAGCAAGGAGAGAAAAG

Downstream 100 bases:

>100_bases
GGCGGCTCCGCACAGCCGCTGTGGGTCGAATTGGGAAAAGCCGATAGTGCGGCTGTCGCAATCGCGATCCTCTGCTCTTC
GCTGAACCTGCGTCGCGTCA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MHPRHFARKAPDRPAIVMAASGETVSYSALENVANRGAQLFRSLGIATGDTIALWLKNCRDYFEIYWAAQRAGLYVCPIA
THLTVEEAAYILNDSGACLLITHADVAAAPDLVSERQRLIPDVRHIFGLEAGMDGFPSWRAALTGFPSKPIADETAGYHL
VYSSGTTGRPKGIRLPLSGEAADAPHMLGDRVAGRYGVGEDSVILSPAPLYHTAPLVYGMVAHRLGATLIVMDHFDAEDM
LRLIERHRVTFIQMVPTMFVRLLALPGTTRAQYDLSSLQKIVHAAAPCPVEIKRRMIDWLGPIIYEYYGGSEGNGSTFIT
PQEWLEHPGSVGRADWGTLHICDEEGNEVPPGVDGLIYFEGGWDFQYLNDPEKTRDARHPRHPAWSTLGDIGHVDKDGYL
YLTDRKGFMIISGGVNIYPQEVENLLITHPRVADAAVIGVPSAEFGEAVKAVVQPLDPADATDEFAEELLRFCRARLSHV
KCPRSIDFDPALPRLDTGKLYKRSIRDRYWPVAPT

Sequences:

>Translated_515_residues
MHPRHFARKAPDRPAIVMAASGETVSYSALENVANRGAQLFRSLGIATGDTIALWLKNCRDYFEIYWAAQRAGLYVCPIA
THLTVEEAAYILNDSGACLLITHADVAAAPDLVSERQRLIPDVRHIFGLEAGMDGFPSWRAALTGFPSKPIADETAGYHL
VYSSGTTGRPKGIRLPLSGEAADAPHMLGDRVAGRYGVGEDSVILSPAPLYHTAPLVYGMVAHRLGATLIVMDHFDAEDM
LRLIERHRVTFIQMVPTMFVRLLALPGTTRAQYDLSSLQKIVHAAAPCPVEIKRRMIDWLGPIIYEYYGGSEGNGSTFIT
PQEWLEHPGSVGRADWGTLHICDEEGNEVPPGVDGLIYFEGGWDFQYLNDPEKTRDARHPRHPAWSTLGDIGHVDKDGYL
YLTDRKGFMIISGGVNIYPQEVENLLITHPRVADAAVIGVPSAEFGEAVKAVVQPLDPADATDEFAEELLRFCRARLSHV
KCPRSIDFDPALPRLDTGKLYKRSIRDRYWPVAPT
>Mature_515_residues
MHPRHFARKAPDRPAIVMAASGETVSYSALENVANRGAQLFRSLGIATGDTIALWLKNCRDYFEIYWAAQRAGLYVCPIA
THLTVEEAAYILNDSGACLLITHADVAAAPDLVSERQRLIPDVRHIFGLEAGMDGFPSWRAALTGFPSKPIADETAGYHL
VYSSGTTGRPKGIRLPLSGEAADAPHMLGDRVAGRYGVGEDSVILSPAPLYHTAPLVYGMVAHRLGATLIVMDHFDAEDM
LRLIERHRVTFIQMVPTMFVRLLALPGTTRAQYDLSSLQKIVHAAAPCPVEIKRRMIDWLGPIIYEYYGGSEGNGSTFIT
PQEWLEHPGSVGRADWGTLHICDEEGNEVPPGVDGLIYFEGGWDFQYLNDPEKTRDARHPRHPAWSTLGDIGHVDKDGYL
YLTDRKGFMIISGGVNIYPQEVENLLITHPRVADAAVIGVPSAEFGEAVKAVVQPLDPADATDEFAEELLRFCRARLSHV
KCPRSIDFDPALPRLDTGKLYKRSIRDRYWPVAPT

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=544, Percent_Identity=26.8382352941176, Blast_Score=172, Evalue=9e-43,
Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=25.981308411215, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI38505220, Length=508, Percent_Identity=25.7874015748031, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI157311624, Length=509, Percent_Identity=25.9332023575639, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI157311622, Length=509, Percent_Identity=25.9332023575639, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI58082049, Length=509, Percent_Identity=26.7190569744597, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI122937307, Length=531, Percent_Identity=27.1186440677966, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI115511026, Length=528, Percent_Identity=23.6742424242424, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=25.0936329588015, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=25.0936329588015, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI38524616, Length=556, Percent_Identity=25.3597122302158, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI227499619, Length=480, Percent_Identity=23.3333333333333, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI8923896, Length=546, Percent_Identity=22.7106227106227, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI28416953, Length=535, Percent_Identity=22.6168224299065, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI253970489, Length=399, Percent_Identity=24.0601503759398, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI13375727, Length=560, Percent_Identity=22.8571428571429, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI13236579, Length=504, Percent_Identity=24.4047619047619, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788107, Length=548, Percent_Identity=28.2846715328467, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI221142682, Length=518, Percent_Identity=28.1853281853282, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI145693145, Length=520, Percent_Identity=28.2692307692308, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1790505, Length=540, Percent_Identity=22.5925925925926, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=24.65483234714, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786529, Length=548, Percent_Identity=22.6277372262774, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=515, Percent_Identity=27.5728155339806, Blast_Score=165, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI32563687, Length=472, Percent_Identity=27.1186440677966, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI71994703, Length=548, Percent_Identity=26.6423357664234, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI71994694, Length=548, Percent_Identity=26.6423357664234, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI71994690, Length=548, Percent_Identity=26.6423357664234, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17560308, Length=546, Percent_Identity=26.9230769230769, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=516, Percent_Identity=27.3255813953488, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=511, Percent_Identity=27.7886497064579, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=534, Percent_Identity=26.9662921348315, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=509, Percent_Identity=27.5049115913556, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17551278, Length=548, Percent_Identity=25.3649635036496, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI115534440, Length=548, Percent_Identity=23.5401459854015, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=367, Percent_Identity=24.2506811989101, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=355, Percent_Identity=25.0704225352113, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319264, Length=559, Percent_Identity=23.4347048300537, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323182, Length=576, Percent_Identity=23.2638888888889, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21355181, Length=509, Percent_Identity=26.3261296660118, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24581924, Length=535, Percent_Identity=26.9158878504673, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859661, Length=532, Percent_Identity=24.812030075188, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI281365686, Length=323, Percent_Identity=26.3157894736842, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356441, Length=467, Percent_Identity=25.2676659528908, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648255, Length=486, Percent_Identity=24.4855967078189, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648253, Length=486, Percent_Identity=24.0740740740741, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648260, Length=357, Percent_Identity=26.3305322128852, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21358303, Length=323, Percent_Identity=26.0061919504644, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922652, Length=516, Percent_Identity=23.2558139534884, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24656500, Length=450, Percent_Identity=23.5555555555556, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648257, Length=356, Percent_Identity=26.123595505618, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24667959, Length=364, Percent_Identity=25.8241758241758, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24667955, Length=364, Percent_Identity=25.8241758241758, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI62472339, Length=364, Percent_Identity=25.8241758241758, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20130357, Length=361, Percent_Identity=24.9307479224377, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356947, Length=516, Percent_Identity=24.031007751938, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355225, Length=375, Percent_Identity=25.0666666666667, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653035, Length=521, Percent_Identity=23.0326295585413, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56848; Mature: 56848

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPRHFARKAPDRPAIVMAASGETVSYSALENVANRGAQLFRSLGIATGDTIALWLKNCR
CCCHHHHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DYFEIYWAAQRAGLYVCPIATHLTVEEAAYILNDSGACLLITHADVAAAPDLVSERQRLI
HHHHHHHHHHHCCEEEECCCHHEEHHHHHHEECCCCCEEEEEECCHHHCHHHHHHHHHHH
PDVRHIFGLEAGMDGFPSWRAALTGFPSKPIADETAGYHLVYSSGTTGRPKGIRLPLSGE
HHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCC
AADAPHMLGDRVAGRYGVGEDSVILSPAPLYHTAPLVYGMVAHRLGATLIVMDHFDAEDM
CCCCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHH
LRLIERHRVTFIQMVPTMFVRLLALPGTTRAQYDLSSLQKIVHAAAPCPVEIKRRMIDWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GPIIYEYYGGSEGNGSTFITPQEWLEHPGSVGRADWGTLHICDEEGNEVPPGVDGLIYFE
HHHHHHHCCCCCCCCCEEECHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEE
GGWDFQYLNDPEKTRDARHPRHPAWSTLGDIGHVDKDGYLYLTDRKGFMIISGGVNIYPQ
CCCCEEECCCCHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCEEEEECCCEECHH
EVENLLITHPRVADAAVIGVPSAEFGEAVKAVVQPLDPADATDEFAEELLRFCRARLSHV
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
KCPRSIDFDPALPRLDTGKLYKRSIRDRYWPVAPT
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHPRHFARKAPDRPAIVMAASGETVSYSALENVANRGAQLFRSLGIATGDTIALWLKNCR
CCCHHHHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DYFEIYWAAQRAGLYVCPIATHLTVEEAAYILNDSGACLLITHADVAAAPDLVSERQRLI
HHHHHHHHHHHCCEEEECCCHHEEHHHHHHEECCCCCEEEEEECCHHHCHHHHHHHHHHH
PDVRHIFGLEAGMDGFPSWRAALTGFPSKPIADETAGYHLVYSSGTTGRPKGIRLPLSGE
HHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCC
AADAPHMLGDRVAGRYGVGEDSVILSPAPLYHTAPLVYGMVAHRLGATLIVMDHFDAEDM
CCCCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHH
LRLIERHRVTFIQMVPTMFVRLLALPGTTRAQYDLSSLQKIVHAAAPCPVEIKRRMIDWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GPIIYEYYGGSEGNGSTFITPQEWLEHPGSVGRADWGTLHICDEEGNEVPPGVDGLIYFE
HHHHHHHCCCCCCCCCEEECHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEE
GGWDFQYLNDPEKTRDARHPRHPAWSTLGDIGHVDKDGYLYLTDRKGFMIISGGVNIYPQ
CCCCEEECCCCHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCEEEEECCCEECHH
EVENLLITHPRVADAAVIGVPSAEFGEAVKAVVQPLDPADATDEFAEELLRFCRARLSHV
HHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
KCPRSIDFDPALPRLDTGKLYKRSIRDRYWPVAPT
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]