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The map label for this gene is menE [H]

Identifier: 103486400

GI number: 103486400

Start: 919864

End: 921369

Strand: Reverse

Name: menE [H]

Synonym: Sala_0910

Alternate gene names: 103486400

Gene position: 921369-919864 (Counterclockwise)

Preceding gene: 103486401

Following gene: 103486399

Centisome position: 27.54

GC content: 65.6

Gene sequence:

>1506_bases
ATGCGAACCTCGCTGTTGCTGGACATTGCCGCTGACGCGGCCTCTGATCGGCCCGCGCTGGGCAGCGGCGAGGAGGCGAT
CGACTTCGGCACCTATCGCGCGCGCGCCAGCCAGACAGCGGCGTGGCTGGCCAGCCGCGGCAAGGCCAACACAGCCTTCC
TCGGCATGAACGGCGAACTGCTGCCCATCCTGCTGTTCGCCAGCGGCATGGCGGGAACAGCCTTTGTGCCCCTGAACTAC
CGCCTCCCCGATGCCGATCTCAACCGGCTGGTCGCGCGCACCGCCCCGGCCGTGCTGATCGCCGACGACGACATGCTGGC
GCGCATCGCCCCGGCCGAAGGTATCGAACTGGTCGCACGGAGCGATTTCGAAGCGGAGTTTCTGGCAGGACCAACGCCGC
CGCCCGCCGCCCTGCCCGAAGCAGAGAATGACATCGCGGTGCTGCTGTTCACCAGCGGCACGACCGGCGAACCGAAAGCC
GCGGTGCTGCGCCACACGAACCTGACCGCCTATGTCATGTCCACGGTGGAGTTTCTGGGCGCGGGCGAGGACGAAGCCGC
GCTGGTCAGCGTTCCTTCCTATCACGTCGCCGGCATTTCGGCGATCCTGACCGCCGCCTATGGCGGACGGCGGATCGTCT
ATCTCCCCGCTTTCACGCCGGACCAATGGGTCGCCACGGCCGCTGCCGAAGCCGTCACCCACGCGATGGTCGTGCCTACG
ATGCTCGACCGCATTCTGGACGTGATGGAACAGACCGGCGAAACGCTACCTGCCTTGCGCGCGCTATCCTATGGTGGCGG
CAAGATGCCGGAGCCGGTGATCGCGCGTGCACTGGCAAAGCTGCCCCACGTCGATTTCGTCAACGCCTATGGCCTGACCG
AAACCAGTTCGACCATCGCCCTGCTGGGCGCGGAGGATCATCGCATCGCCTTTGCGTCCGGCGACCCCGCGATCCGCCGC
CGGATAGCATCGGTCGGGAAGCCGTTGCCAAGTATCGAGCTGGAAATCCGGCGCGAGGACGGAACCCCCTGCGCCCCTGG
GGAGCATGGCGAAATTCACGTCCGCGGCGAGCAGGTTTCGGGCGAATATCTGAACAAAAAGGCGGTGGCCGACGACGGCT
GGTTTGCCACCAATGATGCCGGCTGGCTGGACGAAGGCGGATATCTGTTCGTCGAGGGCCGCCTCGACGATGTGATCGTG
CGCGGGGGCGAGAATATCTCGCCCGGCGAAATCGAAGACCTGCTTCGCACCCATGACGACATTGCCGATTGCGCGGTGCT
GGGCATTCCGTGCGAGAAATGGGGCGAAAAGGTGGTTGCGGTGATTGTCTCGCGATCGGGCCGTCCCGACATCGAAGCGC
TGTCGTCCGCGATCCGCGCGAAGCTTCGTTCGACCAAGACCCCCGAACAATGGTTCGTGCGCGATGCCCTTCCCTATAAT
GAAACGGGCAAGCTGCTGCGGCGCGTGTTGAAGGCGGAACTGGCGGAAGCGGCCTGCGCGGGGTAG

Upstream 100 bases:

>100_bases
AGATACTGGGCGCCAGGGCCGGCAAAGGGTCGCTGGCATTCAACGGCAATCCGCTCGCATTAAAGGGTGCGAAACCGCTC
CCGGCATCGGACTGACACAG

Downstream 100 bases:

>100_bases
GAAGGTGGCGATGACCGGCCCCGACCATCTCGACGCGGATCGCCTGTCCGCGCCGGGCGGGGAACCGGTGCTGGTGGTCG
ATGCAGCGCGCTGGCGCCGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MRTSLLLDIAADAASDRPALGSGEEAIDFGTYRARASQTAAWLASRGKANTAFLGMNGELLPILLFASGMAGTAFVPLNY
RLPDADLNRLVARTAPAVLIADDDMLARIAPAEGIELVARSDFEAEFLAGPTPPPAALPEAENDIAVLLFTSGTTGEPKA
AVLRHTNLTAYVMSTVEFLGAGEDEAALVSVPSYHVAGISAILTAAYGGRRIVYLPAFTPDQWVATAAAEAVTHAMVVPT
MLDRILDVMEQTGETLPALRALSYGGGKMPEPVIARALAKLPHVDFVNAYGLTETSSTIALLGAEDHRIAFASGDPAIRR
RIASVGKPLPSIELEIRREDGTPCAPGEHGEIHVRGEQVSGEYLNKKAVADDGWFATNDAGWLDEGGYLFVEGRLDDVIV
RGGENISPGEIEDLLRTHDDIADCAVLGIPCEKWGEKVVAVIVSRSGRPDIEALSSAIRAKLRSTKTPEQWFVRDALPYN
ETGKLLRRVLKAELAEAACAG

Sequences:

>Translated_501_residues
MRTSLLLDIAADAASDRPALGSGEEAIDFGTYRARASQTAAWLASRGKANTAFLGMNGELLPILLFASGMAGTAFVPLNY
RLPDADLNRLVARTAPAVLIADDDMLARIAPAEGIELVARSDFEAEFLAGPTPPPAALPEAENDIAVLLFTSGTTGEPKA
AVLRHTNLTAYVMSTVEFLGAGEDEAALVSVPSYHVAGISAILTAAYGGRRIVYLPAFTPDQWVATAAAEAVTHAMVVPT
MLDRILDVMEQTGETLPALRALSYGGGKMPEPVIARALAKLPHVDFVNAYGLTETSSTIALLGAEDHRIAFASGDPAIRR
RIASVGKPLPSIELEIRREDGTPCAPGEHGEIHVRGEQVSGEYLNKKAVADDGWFATNDAGWLDEGGYLFVEGRLDDVIV
RGGENISPGEIEDLLRTHDDIADCAVLGIPCEKWGEKVVAVIVSRSGRPDIEALSSAIRAKLRSTKTPEQWFVRDALPYN
ETGKLLRRVLKAELAEAACAG
>Mature_501_residues
MRTSLLLDIAADAASDRPALGSGEEAIDFGTYRARASQTAAWLASRGKANTAFLGMNGELLPILLFASGMAGTAFVPLNY
RLPDADLNRLVARTAPAVLIADDDMLARIAPAEGIELVARSDFEAEFLAGPTPPPAALPEAENDIAVLLFTSGTTGEPKA
AVLRHTNLTAYVMSTVEFLGAGEDEAALVSVPSYHVAGISAILTAAYGGRRIVYLPAFTPDQWVATAAAEAVTHAMVVPT
MLDRILDVMEQTGETLPALRALSYGGGKMPEPVIARALAKLPHVDFVNAYGLTETSSTIALLGAEDHRIAFASGDPAIRR
RIASVGKPLPSIELEIRREDGTPCAPGEHGEIHVRGEQVSGEYLNKKAVADDGWFATNDAGWLDEGGYLFVEGRLDDVIV
RGGENISPGEIEDLLRTHDDIADCAVLGIPCEKWGEKVVAVIVSRSGRPDIEALSSAIRAKLRSTKTPEQWFVRDALPYN
ETGKLLRRVLKAELAEAACAG

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=487, Percent_Identity=28.1314168377823, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI187761343, Length=487, Percent_Identity=28.1314168377823, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI156151445, Length=431, Percent_Identity=26.2180974477958, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI42544132, Length=458, Percent_Identity=23.5807860262009, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI115511026, Length=461, Percent_Identity=25.5965292841649, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI38505220, Length=455, Percent_Identity=24.1758241758242, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI40807357, Length=359, Percent_Identity=25.9052924791086, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI58082049, Length=456, Percent_Identity=24.780701754386, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI157311624, Length=454, Percent_Identity=23.7885462555066, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157311622, Length=454, Percent_Identity=23.7885462555066, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI28416953, Length=130, Percent_Identity=32.3076923076923, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI122937307, Length=461, Percent_Identity=23.644251626898, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI8923896, Length=381, Percent_Identity=24.1469816272966, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI253970489, Length=381, Percent_Identity=24.1469816272966, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI13375727, Length=383, Percent_Identity=27.9373368146214, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI145693145, Length=388, Percent_Identity=28.3505154639175, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788107, Length=368, Percent_Identity=30.9782608695652, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI221142682, Length=452, Percent_Identity=25.4424778761062, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788595, Length=351, Percent_Identity=27.3504273504274, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786810, Length=515, Percent_Identity=24.8543689320388, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786801, Length=498, Percent_Identity=24.6987951807229, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786529, Length=470, Percent_Identity=27.0212765957447, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790505, Length=121, Percent_Identity=32.2314049586777, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=489, Percent_Identity=27.19836400818, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17531443, Length=382, Percent_Identity=28.2722513089005, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32563687, Length=440, Percent_Identity=25.9090909090909, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=373, Percent_Identity=29.7587131367292, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=373, Percent_Identity=29.7587131367292, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71994703, Length=373, Percent_Identity=29.7587131367292, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=355, Percent_Identity=29.5774647887324, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=383, Percent_Identity=27.6762402088773, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=449, Percent_Identity=26.0579064587973, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=442, Percent_Identity=26.6968325791855, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71985884, Length=362, Percent_Identity=28.4530386740331, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25141359, Length=436, Percent_Identity=22.7064220183486, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=369, Percent_Identity=26.5582655826558, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=339, Percent_Identity=25.9587020648968, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115534440, Length=369, Percent_Identity=24.9322493224932, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=27.5482093663912, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323182, Length=563, Percent_Identity=25.5772646536412, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18859661, Length=368, Percent_Identity=29.3478260869565, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24653035, Length=480, Percent_Identity=25.2083333333333, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI161076582, Length=492, Percent_Identity=25.609756097561, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24581924, Length=438, Percent_Identity=29.2237442922374, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI21356947, Length=497, Percent_Identity=25.7545271629779, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355181, Length=464, Percent_Identity=25.4310344827586, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24648255, Length=492, Percent_Identity=22.5609756097561, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356441, Length=435, Percent_Identity=21.6091954022989, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648260, Length=503, Percent_Identity=24.0556660039761, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648253, Length=490, Percent_Identity=22.4489795918367, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21358303, Length=440, Percent_Identity=23.8636363636364, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648257, Length=435, Percent_Identity=24.1379310344828, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922652, Length=491, Percent_Identity=24.847250509165, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20130357, Length=482, Percent_Identity=23.6514522821577, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355225, Length=390, Percent_Identity=26.6666666666667, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281365686, Length=456, Percent_Identity=21.4912280701754, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24656500, Length=371, Percent_Identity=23.7196765498652, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53244; Mature: 53244

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSLLLDIAADAASDRPALGSGEEAIDFGTYRARASQTAAWLASRGKANTAFLGMNGEL
CCCEEEEEEECCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
LPILLFASGMAGTAFVPLNYRLPDADLNRLVARTAPAVLIADDDMLARIAPAEGIELVAR
EEEHEECCCCCCEEEEEEEEECCCHHHHHHHHHCCCEEEEECCCHHEEECCCCCEEEEEC
SDFEAEFLAGPTPPPAALPEAENDIAVLLFTSGTTGEPKAAVLRHTNLTAYVMSTVEFLG
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHC
AGEDEAALVSVPSYHVAGISAILTAAYGGRRIVYLPAFTPDQWVATAAAEAVTHAMVVPT
CCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
MLDRILDVMEQTGETLPALRALSYGGGKMPEPVIARALAKLPHVDFVNAYGLTETSSTIA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCEEE
LLGAEDHRIAFASGDPAIRRRIASVGKPLPSIELEIRREDGTPCAPGEHGEIHVRGEQVS
EEECCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCC
GEYLNKKAVADDGWFATNDAGWLDEGGYLFVEGRLDDVIVRGGENISPGEIEDLLRTHDD
CHHHCCCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHCCC
IADCAVLGIPCEKWGEKVVAVIVSRSGRPDIEALSSAIRAKLRSTKTPEQWFVRDALPYN
HHHHEEECCCHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
ETGKLLRRVLKAELAEAACAG
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTSLLLDIAADAASDRPALGSGEEAIDFGTYRARASQTAAWLASRGKANTAFLGMNGEL
CCCEEEEEEECCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
LPILLFASGMAGTAFVPLNYRLPDADLNRLVARTAPAVLIADDDMLARIAPAEGIELVAR
EEEHEECCCCCCEEEEEEEEECCCHHHHHHHHHCCCEEEEECCCHHEEECCCCCEEEEEC
SDFEAEFLAGPTPPPAALPEAENDIAVLLFTSGTTGEPKAAVLRHTNLTAYVMSTVEFLG
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHC
AGEDEAALVSVPSYHVAGISAILTAAYGGRRIVYLPAFTPDQWVATAAAEAVTHAMVVPT
CCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
MLDRILDVMEQTGETLPALRALSYGGGKMPEPVIARALAKLPHVDFVNAYGLTETSSTIA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCEEE
LLGAEDHRIAFASGDPAIRRRIASVGKPLPSIELEIRREDGTPCAPGEHGEIHVRGEQVS
EEECCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCC
GEYLNKKAVADDGWFATNDAGWLDEGGYLFVEGRLDDVIVRGGENISPGEIEDLLRTHDD
CHHHCCCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHCCC
IADCAVLGIPCEKWGEKVVAVIVSRSGRPDIEALSSAIRAKLRSTKTPEQWFVRDALPYN
HHHHEEECCCHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
ETGKLLRRVLKAELAEAACAG
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA