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The map label for this gene is paaF [C]

Identifier: 103486399

GI number: 103486399

Start: 918870

End: 919853

Strand: Reverse

Name: paaF [C]

Synonym: Sala_0909

Alternate gene names: 103486399

Gene position: 919853-918870 (Counterclockwise)

Preceding gene: 103486400

Following gene: 103486398

Centisome position: 27.5

GC content: 69.31

Gene sequence:

>984_bases
ATGACCGGCCCCGACCATCTCGACGCGGATCGCCTGTCCGCGCCGGGCGGGGAACCGGTGCTGGTGGTCGATGCAGCGCG
CTGGCGCCGACCGCGCGCGCCCGTTCAGGCGGTGGTGATCGGCGTGGACGAAGCGGGCGATCTACCGCCCGTGGTCGCCG
ACGATTTCGACCTGCTGCTGACCGGCGCAATCGCCCCGTCGCGCCCGTGGGTTTCGCTGGCCAGAGCGCCCGGCGCGGAC
ATGCTCGCGACGGCCGTTCGGCAAAGCCCGCTTGCCGCAACCATCGCGGCGCAGGTGCTGCGGATGACCGCCCGGCTTTC
CTTCCCAGATGCACTCGCGGTCGAATCCTTCGCCTATTCCACGCTTCTGGGGGGTTCCGAATTTGCGCGCTGGCGGATGG
GACGCAATCCTGGCGATACCCTTCGGACCCCGGATGATCCATTGCAGATCGAGCGCGAAGGCGACCGGGTTATCGTCACG
CTGAACGATCCGGTCAGCCGCAATGCGATGTCTGCCCCCATGCGCGACGCACTGTATGAAGCGCTGGCCAATATATTGGA
CGACCCCAGCCGCCCTGCCGTCACGCTTTGCGGTGCCGGGCGTTGCTTTTCGAGCGGGGGCGCGCTGGACGAGTTCGGAA
GCGCGCGCGATCTGGCCGAAGCCCATGCCGTCCGCACGCTCCATAGCTGCGCCCGCGCGCTCGACGCGATCGGCGAGCGC
GGCGCCGTCCGCCTGCATGGCGCCTGCATCGGCTCCGGGATCGAAATCGCCGCCGCCGCGCATCGGCGCATCGCAGCCCC
CGACGCCTGGTTTCAGCTCCCGGAACTCGGCATGGGGCTCATTCCCGGCGCGGGCGGCACCATCAGCATCGCGCGGGCGA
TCGGTCGGCACCGGACGCTTTGGATGCTGCTCTCGGGCCGACGGATCAAAGCGCCCACGGCGCTGGAATGGGGACTGGTC
CATCATGTCGAGGAGGCGGCTTGA

Upstream 100 bases:

>100_bases
GTGCGCGATGCCCTTCCCTATAATGAAACGGGCAAGCTGCTGCGGCGCGTGTTGAAGGCGGAACTGGCGGAAGCGGCCTG
CGCGGGGTAGGAAGGTGGCG

Downstream 100 bases:

>100_bases
CCGCCTTGCTGGACGCCGTGGCGACCGCAGGCGAGCGGTTGATCGCCGCGTCGGACGGCCGCATCGCGTTCGATCCGCGC
CGCGCGCTATCGCGCGCCGA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTGPDHLDADRLSAPGGEPVLVVDAARWRRPRAPVQAVVIGVDEAGDLPPVVADDFDLLLTGAIAPSRPWVSLARAPGAD
MLATAVRQSPLAATIAAQVLRMTARLSFPDALAVESFAYSTLLGGSEFARWRMGRNPGDTLRTPDDPLQIEREGDRVIVT
LNDPVSRNAMSAPMRDALYEALANILDDPSRPAVTLCGAGRCFSSGGALDEFGSARDLAEAHAVRTLHSCARALDAIGER
GAVRLHGACIGSGIEIAAAAHRRIAAPDAWFQLPELGMGLIPGAGGTISIARAIGRHRTLWMLLSGRRIKAPTALEWGLV
HHVEEAA

Sequences:

>Translated_327_residues
MTGPDHLDADRLSAPGGEPVLVVDAARWRRPRAPVQAVVIGVDEAGDLPPVVADDFDLLLTGAIAPSRPWVSLARAPGAD
MLATAVRQSPLAATIAAQVLRMTARLSFPDALAVESFAYSTLLGGSEFARWRMGRNPGDTLRTPDDPLQIEREGDRVIVT
LNDPVSRNAMSAPMRDALYEALANILDDPSRPAVTLCGAGRCFSSGGALDEFGSARDLAEAHAVRTLHSCARALDAIGER
GAVRLHGACIGSGIEIAAAAHRRIAAPDAWFQLPELGMGLIPGAGGTISIARAIGRHRTLWMLLSGRRIKAPTALEWGLV
HHVEEAA
>Mature_326_residues
TGPDHLDADRLSAPGGEPVLVVDAARWRRPRAPVQAVVIGVDEAGDLPPVVADDFDLLLTGAIAPSRPWVSLARAPGADM
LATAVRQSPLAATIAAQVLRMTARLSFPDALAVESFAYSTLLGGSEFARWRMGRNPGDTLRTPDDPLQIEREGDRVIVTL
NDPVSRNAMSAPMRDALYEALANILDDPSRPAVTLCGAGRCFSSGGALDEFGSARDLAEAHAVRTLHSCARALDAIGERG
AVRLHGACIGSGIEIAAAAHRRIAAPDAWFQLPELGMGLIPGAGGTISIARAIGRHRTLWMLLSGRRIKAPTALEWGLVH
HVEEAA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=30.2197802197802, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI194097323, Length=166, Percent_Identity=30.1204819277108, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI68989263, Length=164, Percent_Identity=33.5365853658537, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1787659, Length=176, Percent_Identity=32.3863636363636, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788682, Length=171, Percent_Identity=29.2397660818713, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI221142681, Length=178, Percent_Identity=27.5280898876405, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=181, Percent_Identity=28.7292817679558, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129971, Length=174, Percent_Identity=29.3103448275862, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653477, Length=174, Percent_Identity=29.3103448275862, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 34549; Mature: 34418

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPDHLDADRLSAPGGEPVLVVDAARWRRPRAPVQAVVIGVDEAGDLPPVVADDFDLLL
CCCCCCCCHHHHCCCCCCEEEEEEHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEE
TGAIAPSRPWVSLARAPGADMLATAVRQSPLAATIAAQVLRMTARLSFPDALAVESFAYS
ECCCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TLLGGSEFARWRMGRNPGDTLRTPDDPLQIEREGDRVIVTLNDPVSRNAMSAPMRDALYE
HHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCHHCCHHHHHHHH
ALANILDDPSRPAVTLCGAGRCFSSGGALDEFGSARDLAEAHAVRTLHSCARALDAIGER
HHHHHHCCCCCCEEEEECCCCEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GAVRLHGACIGSGIEIAAAAHRRIAAPDAWFQLPELGMGLIPGAGGTISIARAIGRHRTL
CCEEEECEEECCCCHHHHHHHHHCCCCHHHHCCHHCCCCCCCCCCCHHHHHHHHCCCCEE
WMLLSGRRIKAPTALEWGLVHHVEEAA
EEEECCCEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGPDHLDADRLSAPGGEPVLVVDAARWRRPRAPVQAVVIGVDEAGDLPPVVADDFDLLL
CCCCCCCHHHHCCCCCCEEEEEEHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEE
TGAIAPSRPWVSLARAPGADMLATAVRQSPLAATIAAQVLRMTARLSFPDALAVESFAYS
ECCCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TLLGGSEFARWRMGRNPGDTLRTPDDPLQIEREGDRVIVTLNDPVSRNAMSAPMRDALYE
HHHCCHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCHHCCHHHHHHHH
ALANILDDPSRPAVTLCGAGRCFSSGGALDEFGSARDLAEAHAVRTLHSCARALDAIGER
HHHHHHCCCCCCEEEEECCCCEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GAVRLHGACIGSGIEIAAAAHRRIAAPDAWFQLPELGMGLIPGAGGTISIARAIGRHRTL
CCEEEECEEECCCCHHHHHHHHHCCCCHHHHCCHHCCCCCCCCCCCHHHHHHHHCCCCEE
WMLLSGRRIKAPTALEWGLVHHVEEAA
EEEECCCEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA