BacMap

Definition	Sphingopyxis alaskensis RB2256, complete genome.
Accession	NC_008048
Length	3,345,170

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The map label for this gene is fadH [H]

Identifier: 103486367

GI number: 103486367

Start: 883713

End: 884624

Strand: Reverse

Name: fadH [H]

Synonym: Sala_0877

Alternate gene names: 103486367

Gene position: 884624-883713 (Counterclockwise)

Preceding gene: 103486368

Following gene: 103486366

Centisome position: 26.44

GC content: 67.98

Gene sequence:

>912_bases
ATGGCGCTGCCCGCCCCGCCCCCGCTCGGTGCCAAGGCGCTGCCCGACGGCACCTATGCGGGCCAGGTCGTGGCCGTTAC
CGGCGGGGGCACCGGGCTGGGCAAGGGCATGGCGATCGAGTTTGCGCGGCTGGGCGCGAAGGTCGCGATACTTAGCCGCA
AGCCCGACCATCTCGACGCCGGCGTCGCGGCGATCCGCGCGCTGGGCGCGGAGGCGATCGCCGTCGCCTGCGACGTGCGC
GACGCGGACGCTGTCGCGCAGGCGTTCGACACAATCGAAGCGAAACTGGGCGCGGTCGATGTGCTCGTCAATAATGCGGC
GGGCAATTTTCCGGCCCCGGCAGAAGAAATGACGCCGAACGGCTTTCGCACCGTCGTCGATATCGTCCTGAACGGCACCT
ATAATTGCAGCCGCGAATTCGCGATACGGCGACTGGCAGCGAAACTGCCGGGCGCGATATTGAACATCGGCGCCACCTAT
AGCTGGACGGGCGGGCCGGGGACGTCGCATTCGGCGGCGGCCAAGGCGGGCGTGACCAACCTGACGCAGAGCCTGGCGGT
CGAGTGGGCGCCCGACGGAATCCGCGTCAATTGCATCGCGCCTGGCCGTTTTCCGCACGACGATCTGCCCGCGCACATGA
CCCGCCATCGCGAGGGCGAGCGCGGCGACAATACGATTCCGGGAATGCGCGTCGGCGAGGTGCGCGAACTGGGCTGGGCG
GCGACCTTCCTCTGTTCGCCCTATGCCAGCTATATCAGCGGTCACACGCTGGTCGTCGATGCGGCGAACTGGCTGCGGCG
CAGCCTGGTGATGCCCGAGTTTGTGCCGATCCGCGAACAATTTGGACGGCGTGCCGTCGATAGCGGCAGCGCCCGCGCCG
CCATTGCCAAAACCAGAGGGGACCGGCCATGA

Upstream 100 bases:

>100_bases
GAGCTTTATGCGCAAAGCTATCTGTTCCAGACCGAAGATTATCGGGAAGCCCGCACGGCGCTGCGCGAACGGCGCCAACC
CCGGTACAGGGGGAAATAGC

Downstream 100 bases:

>100_bases
GCAAACCGCTCGTCATCCGGGAGGACTCCGGCGGCATCTGTACGCTGACGCTCAACCGCCCCGACAAGCGCAATGCCATC
AACCGTGACCTGTTCCGTGC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MALPAPPPLGAKALPDGTYAGQVVAVTGGGTGLGKGMAIEFARLGAKVAILSRKPDHLDAGVAAIRALGAEAIAVACDVR
DADAVAQAFDTIEAKLGAVDVLVNNAAGNFPAPAEEMTPNGFRTVVDIVLNGTYNCSREFAIRRLAAKLPGAILNIGATY
SWTGGPGTSHSAAAKAGVTNLTQSLAVEWAPDGIRVNCIAPGRFPHDDLPAHMTRHREGERGDNTIPGMRVGEVRELGWA
ATFLCSPYASYISGHTLVVDAANWLRRSLVMPEFVPIREQFGRRAVDSGSARAAIAKTRGDRP

Sequences:

>Translated_303_residues
MALPAPPPLGAKALPDGTYAGQVVAVTGGGTGLGKGMAIEFARLGAKVAILSRKPDHLDAGVAAIRALGAEAIAVACDVR
DADAVAQAFDTIEAKLGAVDVLVNNAAGNFPAPAEEMTPNGFRTVVDIVLNGTYNCSREFAIRRLAAKLPGAILNIGATY
SWTGGPGTSHSAAAKAGVTNLTQSLAVEWAPDGIRVNCIAPGRFPHDDLPAHMTRHREGERGDNTIPGMRVGEVRELGWA
ATFLCSPYASYISGHTLVVDAANWLRRSLVMPEFVPIREQFGRRAVDSGSARAAIAKTRGDRP
>Mature_302_residues
ALPAPPPLGAKALPDGTYAGQVVAVTGGGTGLGKGMAIEFARLGAKVAILSRKPDHLDAGVAAIRALGAEAIAVACDVRD
ADAVAQAFDTIEAKLGAVDVLVNNAAGNFPAPAEEMTPNGFRTVVDIVLNGTYNCSREFAIRRLAAKLPGAILNIGATYS
WTGGPGTSHSAAAKAGVTNLTQSLAVEWAPDGIRVNCIAPGRFPHDDLPAHMTRHREGERGDNTIPGMRVGEVRELGWAA
TFLCSPYASYISGHTLVVDAANWLRRSLVMPEFVPIREQFGRRAVDSGSARAAIAKTRGDRP

Specific function: Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3- enoyl-CoA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=279, Percent_Identity=38.7096774193548, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI19923817, Length=287, Percent_Identity=34.4947735191638, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI10190704, Length=284, Percent_Identity=35.2112676056338, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=34.4, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI32483357, Length=242, Percent_Identity=29.3388429752066, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI7705925, Length=246, Percent_Identity=30.4878048780488, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI59889578, Length=225, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=28.0487804878049, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI126723750, Length=246, Percent_Identity=25.609756097561, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4758504, Length=199, Percent_Identity=31.1557788944724, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=28.7179487179487, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4504505, Length=193, Percent_Identity=29.5336787564767, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI87082100, Length=267, Percent_Identity=31.4606741573034, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787905, Length=239, Percent_Identity=33.4728033472803, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=30.6772908366534, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787335, Length=249, Percent_Identity=31.3253012048193, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI2367175, Length=238, Percent_Identity=32.3529411764706, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082160, Length=258, Percent_Identity=28.6821705426357, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790717, Length=243, Percent_Identity=28.3950617283951, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788459, Length=257, Percent_Identity=28.7937743190661, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786812, Length=259, Percent_Identity=26.2548262548263, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789057, Length=250, Percent_Identity=26.4, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789378, Length=232, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17561272, Length=259, Percent_Identity=40.5405405405405, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17536651, Length=255, Percent_Identity=37.6470588235294, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17536025, Length=262, Percent_Identity=37.0229007633588, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17555706, Length=243, Percent_Identity=30.8641975308642, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25147288, Length=246, Percent_Identity=30.4878048780488, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560676, Length=238, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=290, Percent_Identity=27.9310344827586, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562910, Length=278, Percent_Identity=25.5395683453237, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17562990, Length=286, Percent_Identity=26.9230769230769, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=275, Percent_Identity=28, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17567345, Length=191, Percent_Identity=29.8429319371728, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=294, Percent_Identity=25.1700680272109, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=295, Percent_Identity=25.4237288135593, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538182, Length=193, Percent_Identity=33.160621761658, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=269, Percent_Identity=29.368029739777, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=275, Percent_Identity=26.9090909090909, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=277, Percent_Identity=24.5487364620939, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=252, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17555612, Length=207, Percent_Identity=28.5024154589372, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=174, Percent_Identity=28.735632183908, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=173, Percent_Identity=28.9017341040462, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=266, Percent_Identity=24.0601503759398, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=278, Percent_Identity=25.5395683453237, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=263, Percent_Identity=25.8555133079848, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=265, Percent_Identity=24.5283018867925, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=272, Percent_Identity=24.6323529411765, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71997099, Length=202, Percent_Identity=27.7227722772277, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=259, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=262, Percent_Identity=25.5725190839695, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=266, Percent_Identity=33.8345864661654, Blast_Score=145, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6322861, Length=248, Percent_Identity=29.4354838709677, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21355319, Length=241, Percent_Identity=29.4605809128631, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI23397609, Length=248, Percent_Identity=31.4516129032258, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=30.2788844621514, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=31.2, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643142, Length=253, Percent_Identity=29.6442687747036, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644339, Length=249, Percent_Identity=30.5220883534137, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571526, Length=255, Percent_Identity=28.6274509803922, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24584752, Length=180, Percent_Identity=30, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644337, Length=264, Percent_Identity=28.4090909090909, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI18859837, Length=192, Percent_Identity=30.7291666666667, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17737361, Length=199, Percent_Identity=31.6582914572864, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641232, Length=206, Percent_Identity=29.6116504854369, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007234, Length=204, Percent_Identity=28.921568627451, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.34 [H]

Molecular weight: Translated: 31611; Mature: 31480

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPAPPPLGAKALPDGTYAGQVVAVTGGGTGLGKGMAIEFARLGAKVAILSRKPDHLDA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCCCCHHHH
GVAAIRALGAEAIAVACDVRDADAVAQAFDTIEAKLGAVDVLVNNAAGNFPAPAEEMTPN
HHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHCCCC
GFRTVVDIVLNGTYNCSREFAIRRLAAKLPGAILNIGATYSWTGGPGTSHSAAAKAGVTN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCHHHHHHHHHH
LTQSLAVEWAPDGIRVNCIAPGRFPHDDLPAHMTRHREGERGDNTIPGMRVGEVRELGWA
HHHHHEEEECCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
ATFLCSPYASYISGHTLVVDAANWLRRSLVMPEFVPIREQFGRRAVDSGSARAAIAKTRG
HHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCHHHHHHCCHHHCCCCCCEEHHHCCC
DRP
CCC
>Mature Secondary Structure 
ALPAPPPLGAKALPDGTYAGQVVAVTGGGTGLGKGMAIEFARLGAKVAILSRKPDHLDA
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCCCCHHHH
GVAAIRALGAEAIAVACDVRDADAVAQAFDTIEAKLGAVDVLVNNAAGNFPAPAEEMTPN
HHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHCCCC
GFRTVVDIVLNGTYNCSREFAIRRLAAKLPGAILNIGATYSWTGGPGTSHSAAAKAGVTN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCCHHHHHHHHHH
LTQSLAVEWAPDGIRVNCIAPGRFPHDDLPAHMTRHREGERGDNTIPGMRVGEVRELGWA
HHHHHEEEECCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
ATFLCSPYASYISGHTLVVDAANWLRRSLVMPEFVPIREQFGRRAVDSGSARAAIAKTRG
HHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCCHHHHHHCCHHHCCCCCCEEHHHCCC
DRP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]