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The map label for this gene is ccsA [H]

Identifier: 103486319

GI number: 103486319

Start: 833336

End: 834619

Strand: Reverse

Name: ccsA [H]

Synonym: Sala_0829

Alternate gene names: 103486319

Gene position: 834619-833336 (Counterclockwise)

Preceding gene: 103486320

Following gene: 103486317

Centisome position: 24.95

GC content: 63.55

Gene sequence:

>1284_bases
ATGACCGACACCGCGAAAATCACGCTGGGCGACAAGACCGTCGACAGCCCTGTCCTGTCGGGCACCGTCGGCCCCGATGT
GGTCGACATCCGCAAATTCTATGCCCAGACCGGCGCCTTCACCTATGACCCCGGCTTCACCTCGACCGCGAGCTGCGAAT
CGAAGCTGACCTATATCGACGGCGACGAAGGCGTGCTGCTGCACCGCGGTTACGCGATCGGCGACCTCGCCGAACATTCG
TCGTTCATGGAGGTGTGCTACCTCCTGCTCAACGGCGAACTGCCGACCGCCGACGAGCTCAAGACGTTCGACAATACGAT
CACCCGCCACACGATGCTGCACGAACAGCTCGCGACCTTTTACCGCGGGTTCCGCCGCGACGCGCACCCGATGGCGATCA
TGTGCGGCGTCGTCGGCGCGCTCAGCGCCTTTTATCACGACTCGACCGACATTCAGGACCCGCACCAGCGGATGATCGCC
AGCCACCGGCTGATCGCCAAGATGCCGACGATCGCGGCGATGGCGTATAAATATTCGGTGGGTCAGCCCTTCATCTACCC
CGACAACAAGCTCAGCTACACGGGCAATTTCCTGCGCATGACCTTCGGCGTTCCGGCCGAGGAATATGAGGTGGTGCCCG
CCGTCGAGCGCGCGCTCGACCGCATCTTCATCCTCCATGCCGACCATGAGCAGAATGCGTCGACCTCGACCGTCCGCCTG
GCGGGTTCGTCGGGCGCCAACCCCTTTGCGTGCATCGCCGCGGGCATCGCGTGCCTGTGGGGCCCGGCGCACGGCGGCGC
GAACGAGGCCGCGCTCAATATGCTGCGCGAGATCGGCCGCCCCGAACGCATCCCCGAATATATCGCGCGCGCCAAGGACA
AGGACGACCCGTTCCGCCTGATGGGGTTCGGCCACCGCGTGTACAAAAATTACGACCCGCGCGCGACGGTGATGCAGAAA
ACGGTGCGCGAAGTGTTCGACGCGCTGAAGGTCAACGACCCGGTGTTCGAGGTCGCGCTGCAACTGGAGGAAATGGCGCT
GAACGACGATTATTTCGTCGAGAAAAAGCTGTTCCCCAATGTCGATTTCTATTCGGGCGTGATCCTGTCGGCGATCGGTT
TCCCGACGACGATGTTCACCGCGCTGTTCGCGCTCGCCCGCACCGTCGGCTGGGTCGCGCAGTGGAACGAGATGATTTCG
GACCCCGCACAGAAAATCGGCCGCCCGCGCCAGCTTTACACCGGCCCGACGCACCGGCCCTATGTGCCGGTGAGCCAGCG
CTAA

Upstream 100 bases:

>100_bases
TGGGCCGCAAAGTCAGCCTGGCGCGACTTGACGCAGCGCAACATTATCCGGCAGGGGCGTAGCCGCAACATCTCCCCGCC
GTCAGAACAGGGAACCTAAT

Downstream 100 bases:

>100_bases
AACGGCGTGCGTTTAATCTGAGTCATCGTCCCGTTCGTGTCGAGCGAAGTCGAGACACCCATCGGCGTGGCGCAAGATCG
ATGGGTGTCTCGACTTCGCT

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTDTAKITLGDKTVDSPVLSGTVGPDVVDIRKFYAQTGAFTYDPGFTSTASCESKLTYIDGDEGVLLHRGYAIGDLAEHS
SFMEVCYLLLNGELPTADELKTFDNTITRHTMLHEQLATFYRGFRRDAHPMAIMCGVVGALSAFYHDSTDIQDPHQRMIA
SHRLIAKMPTIAAMAYKYSVGQPFIYPDNKLSYTGNFLRMTFGVPAEEYEVVPAVERALDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLREIGRPERIPEYIARAKDKDDPFRLMGFGHRVYKNYDPRATVMQK
TVREVFDALKVNDPVFEVALQLEEMALNDDYFVEKKLFPNVDFYSGVILSAIGFPTTMFTALFALARTVGWVAQWNEMIS
DPAQKIGRPRQLYTGPTHRPYVPVSQR

Sequences:

>Translated_427_residues
MTDTAKITLGDKTVDSPVLSGTVGPDVVDIRKFYAQTGAFTYDPGFTSTASCESKLTYIDGDEGVLLHRGYAIGDLAEHS
SFMEVCYLLLNGELPTADELKTFDNTITRHTMLHEQLATFYRGFRRDAHPMAIMCGVVGALSAFYHDSTDIQDPHQRMIA
SHRLIAKMPTIAAMAYKYSVGQPFIYPDNKLSYTGNFLRMTFGVPAEEYEVVPAVERALDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLREIGRPERIPEYIARAKDKDDPFRLMGFGHRVYKNYDPRATVMQK
TVREVFDALKVNDPVFEVALQLEEMALNDDYFVEKKLFPNVDFYSGVILSAIGFPTTMFTALFALARTVGWVAQWNEMIS
DPAQKIGRPRQLYTGPTHRPYVPVSQR
>Mature_426_residues
TDTAKITLGDKTVDSPVLSGTVGPDVVDIRKFYAQTGAFTYDPGFTSTASCESKLTYIDGDEGVLLHRGYAIGDLAEHSS
FMEVCYLLLNGELPTADELKTFDNTITRHTMLHEQLATFYRGFRRDAHPMAIMCGVVGALSAFYHDSTDIQDPHQRMIAS
HRLIAKMPTIAAMAYKYSVGQPFIYPDNKLSYTGNFLRMTFGVPAEEYEVVPAVERALDRIFILHADHEQNASTSTVRLA
GSSGANPFACIAAGIACLWGPAHGGANEAALNMLREIGRPERIPEYIARAKDKDDPFRLMGFGHRVYKNYDPRATVMQKT
VREVFDALKVNDPVFEVALQLEEMALNDDYFVEKKLFPNVDFYSGVILSAIGFPTTMFTALFALARTVGWVAQWNEMISD
PAQKIGRPRQLYTGPTHRPYVPVSQR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=404, Percent_Identity=27.4752475247525, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786939, Length=423, Percent_Identity=66.4302600472813, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=34.2245989304813, Blast_Score=174, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17555174, Length=396, Percent_Identity=25.2525252525253, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=356, Percent_Identity=28.3707865168539, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319850, Length=360, Percent_Identity=28.0555555555556, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=372, Percent_Identity=25.2688172043011, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640126, Length=438, Percent_Identity=27.8538812785388, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24640124, Length=438, Percent_Identity=28.0821917808219, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356863, Length=362, Percent_Identity=26.7955801104972, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47509; Mature: 47377

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTAKITLGDKTVDSPVLSGTVGPDVVDIRKFYAQTGAFTYDPGFTSTASCESKLTYID
CCCCEEEEECCCCCCCCHHCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEE
GDEGVLLHRGYAIGDLAEHSSFMEVCYLLLNGELPTADELKTFDNTITRHTMLHEQLATF
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YRGFRRDAHPMAIMCGVVGALSAFYHDSTDIQDPHQRMIASHRLIAKMPTIAAMAYKYSV
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCC
GQPFIYPDNKLSYTGNFLRMTFGVPAEEYEVVPAVERALDRIFILHADHEQNASTSTVRL
CCCEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEE
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLREIGRPERIPEYIARAKDKDDPFRL
ECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
MGFGHRVYKNYDPRATVMQKTVREVFDALKVNDPVFEVALQLEEMALNDDYFVEKKLFPN
HHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHHHCCCC
VDFYSGVILSAIGFPTTMFTALFALARTVGWVAQWNEMISDPAQKIGRPRQLYTGPTHRP
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEECCCCCCCC
YVPVSQR
CCCCCCC
>Mature Secondary Structure 
TDTAKITLGDKTVDSPVLSGTVGPDVVDIRKFYAQTGAFTYDPGFTSTASCESKLTYID
CCCEEEEECCCCCCCCHHCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEE
GDEGVLLHRGYAIGDLAEHSSFMEVCYLLLNGELPTADELKTFDNTITRHTMLHEQLATF
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YRGFRRDAHPMAIMCGVVGALSAFYHDSTDIQDPHQRMIASHRLIAKMPTIAAMAYKYSV
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCC
GQPFIYPDNKLSYTGNFLRMTFGVPAEEYEVVPAVERALDRIFILHADHEQNASTSTVRL
CCCEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEE
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLREIGRPERIPEYIARAKDKDDPFRL
ECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
MGFGHRVYKNYDPRATVMQKTVREVFDALKVNDPVFEVALQLEEMALNDDYFVEKKLFPN
HHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHHHCCCC
VDFYSGVILSAIGFPTTMFTALFALARTVGWVAQWNEMISDPAQKIGRPRQLYTGPTHRP
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEECCCCCCCC
YVPVSQR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]