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The map label for this gene is phoB [H]

Identifier: 103486311

GI number: 103486311

Start: 824797

End: 825495

Strand: Reverse

Name: phoB [H]

Synonym: Sala_0821

Alternate gene names: 103486311

Gene position: 825495-824797 (Counterclockwise)

Preceding gene: 103486312

Following gene: 103486308

Centisome position: 24.68

GC content: 66.52

Gene sequence:

>699_bases
ATGCCCCAGCCCGATCTGCTGCTGATCGAGGATGACGAGGCGATCGCCGAACTCATCGTCTGGCATTTCGCGCGCGAGGG
CTTTTCGGTCCGTCAGACCCCCGATGGCGAGCAGGCGCTGGTGCTGGTCGAGGAGCGCGTTCCCGACATCGTCCTGCTCG
ACTGGATGATCGAGAGCCTGCCGGGCATCGAGGTCTGCCGCCGCCTGCGCCGCAATCCCAAATCGGCGAACGTGCCGATC
ATCATGCTCACCGCGCGCGGCGAGGAAGAGGACCGCATCCGCGGGCTCGAAACCGGCGCCGATGATTATGTGACCAAGCC
GTTCAGCCCGCGCGAACTGGTCGCGCGCGTGTCGGCGGTGCTTCGCCGCCTGCGCCCGGCGCTCGCTGGCGAAATGCTGA
GCTATGCCGACATCGAGCTCGATTCGGTCGCGCACAAGGTCGTCCGCGGCGGTCAGGTCGTCACGATGGGGCCGACCGAG
TTCCGCCTGCTCAGGCATTTCATGGAACATCCGGGCCGCGTCTTTTCGCGCGGGCAATTGCTCGACAGCGTGTGGGGCCA
GGACAGCGACATCGAACTGCGCACGGTCGACGTCCATATCCGGCGGCTGCGCAAGGCGATCAACCTGCCGGGAACGAGCG
ACATCATCCGCACCGTGCGCTCGGCGGGCTATGCGCTGGACGCGGGCAAGAGCGTTTGA

Upstream 100 bases:

>100_bases
CCGAGATGGTCTATTATGTCGTGACCGGCGAACATATGGAGGAGCGCGAGCGCGGGGAAACCCCCGAGGAAAGCGCCGCG
GCGGAGAAGGAGCAAGGCTG

Downstream 100 bases:

>100_bases
TGGGGCGGCCGCGGACCGGCCGGAGGCAGGCCATCATCATGCCGGACCTGGCGAAAGTTGGCGTATGTCGGGTAAACGAC
CTATTCTGTTGAAGAAGTCG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MPQPDLLLIEDDEAIAELIVWHFAREGFSVRQTPDGEQALVLVEERVPDIVLLDWMIESLPGIEVCRRLRRNPKSANVPI
IMLTARGEEEDRIRGLETGADDYVTKPFSPRELVARVSAVLRRLRPALAGEMLSYADIELDSVAHKVVRGGQVVTMGPTE
FRLLRHFMEHPGRVFSRGQLLDSVWGQDSDIELRTVDVHIRRLRKAINLPGTSDIIRTVRSAGYALDAGKSV

Sequences:

>Translated_232_residues
MPQPDLLLIEDDEAIAELIVWHFAREGFSVRQTPDGEQALVLVEERVPDIVLLDWMIESLPGIEVCRRLRRNPKSANVPI
IMLTARGEEEDRIRGLETGADDYVTKPFSPRELVARVSAVLRRLRPALAGEMLSYADIELDSVAHKVVRGGQVVTMGPTE
FRLLRHFMEHPGRVFSRGQLLDSVWGQDSDIELRTVDVHIRRLRKAINLPGTSDIIRTVRSAGYALDAGKSV
>Mature_231_residues
PQPDLLLIEDDEAIAELIVWHFAREGFSVRQTPDGEQALVLVEERVPDIVLLDWMIESLPGIEVCRRLRRNPKSANVPII
MLTARGEEEDRIRGLETGADDYVTKPFSPRELVARVSAVLRRLRPALAGEMLSYADIELDSVAHKVVRGGQVVTMGPTEF
RLLRHFMEHPGRVFSRGQLLDSVWGQDSDIELRTVDVHIRRLRKAINLPGTSDIIRTVRSAGYALDAGKSV

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=48.8584474885845, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI1788394, Length=230, Percent_Identity=36.9565217391304, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790860, Length=230, Percent_Identity=40.4347826086956, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=36.036036036036, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=35.5263157894737, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=35.5555555555556, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=34.7639484978541, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=34.3891402714932, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786911, Length=204, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787229, Length=235, Percent_Identity=33.1914893617021, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=31.1111111111111, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=36.7521367521368, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=30.6666666666667, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=30.2127659574468, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=28.3333333333333, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26093; Mature: 25962

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQPDLLLIEDDEAIAELIVWHFAREGFSVRQTPDGEQALVLVEERVPDIVLLDWMIESL
CCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHC
PGIEVCRRLRRNPKSANVPIIMLTARGEEEDRIRGLETGADDYVTKPFSPRELVARVSAV
CCHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LRRLRPALAGEMLSYADIELDSVAHKVVRGGQVVTMGPTEFRLLRHFMEHPGRVFSRGQL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHH
LDSVWGQDSDIELRTVDVHIRRLRKAINLPGTSDIIRTVRSAGYALDAGKSV
HHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PQPDLLLIEDDEAIAELIVWHFAREGFSVRQTPDGEQALVLVEERVPDIVLLDWMIESL
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHC
PGIEVCRRLRRNPKSANVPIIMLTARGEEEDRIRGLETGADDYVTKPFSPRELVARVSAV
CCHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LRRLRPALAGEMLSYADIELDSVAHKVVRGGQVVTMGPTEFRLLRHFMEHPGRVFSRGQL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHH
LDSVWGQDSDIELRTVDVHIRRLRKAINLPGTSDIIRTVRSAGYALDAGKSV
HHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]