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The map label for this gene is ligT [C]

Identifier: 103486305

GI number: 103486305

Start: 819123

End: 819737

Strand: Direct

Name: ligT [C]

Synonym: Sala_0814

Alternate gene names: 103486305

Gene position: 819123-819737 (Clockwise)

Preceding gene: 103486303

Following gene: 103486307

Centisome position: 24.49

GC content: 69.43

Gene sequence:

>615_bases
ATGTCGACGCACCGCCTGTTCGTCGCGCTGCGACCGCCGCCGCCCGTTCGCGCACTTTTGCTGCGCGCGATGCACGGCAT
TTCGGGCGCGCGCTGGCAAAGCGACGAGCAGCTTCACCTGACGCTGCGCTTCATCGGCGAGGTCGACCACCATCTGGCCG
AAGACATTGCGGCGGTGCTGGGCGCGCTCCACGCACCGGCGGCGAGGGTGCGTATCGCCGGCGTCGACCTGTTCGAGCGG
CAGGGGCGACCGCACATGGTCTGGGCGCGCATCGCCCCCGAAGAGCCGCTCGCCGCGCTGCACCGCAAGGTCGAACAATG
TCTGGCGCGCGTCGGCATCGCGGCCGAAACCCGCGCCTTCGTGCCGCATGTCACGCTGGCGCGGCTCAACCGGTCATCGG
GGCCGGTCGCGCCCTTTCTCGCGCTGAACGGAGATCTTGCGAGCGACCCCTTTCTGTTCGACCATGTCACGCTTTACGAG
AGCGAAATGGGGCACGGCGGCTCACGCTATCACCCCGTCGCGCGCTATCCGCTCGTCGACCAGCGCGCGGCGAGCGCCGA
AGCGACCGCCGCCACATCCTCCCACGTCGCGGCAAAGCCCTCGTCCGAGCCATAA

Upstream 100 bases:

>100_bases
GATTCAAGCGAAACCGGACAGGCGCGACCGGACGGCCTTAACGATGCGGCAAATCGGCCGGGGAGTCGCGCCGCGCGGCG
AGACGCCCTATGCTGTGATC

Downstream 100 bases:

>100_bases
TAGGGATCGGTCACCCCCTGCCCCGCGCGTCCGGCAACGAGGTCGAGCATCAGGCATAGCTCGGCGGTCGCGTCGTCGGG
GCGGATTGCCAGCGCGTCGC

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSTHRLFVALRPPPPVRALLLRAMHGISGARWQSDEQLHLTLRFIGEVDHHLAEDIAAVLGALHAPAARVRIAGVDLFER
QGRPHMVWARIAPEEPLAALHRKVEQCLARVGIAAETRAFVPHVTLARLNRSSGPVAPFLALNGDLASDPFLFDHVTLYE
SEMGHGGSRYHPVARYPLVDQRAASAEATAATSSHVAAKPSSEP

Sequences:

>Translated_204_residues
MSTHRLFVALRPPPPVRALLLRAMHGISGARWQSDEQLHLTLRFIGEVDHHLAEDIAAVLGALHAPAARVRIAGVDLFER
QGRPHMVWARIAPEEPLAALHRKVEQCLARVGIAAETRAFVPHVTLARLNRSSGPVAPFLALNGDLASDPFLFDHVTLYE
SEMGHGGSRYHPVARYPLVDQRAASAEATAATSSHVAAKPSSEP
>Mature_203_residues
STHRLFVALRPPPPVRALLLRAMHGISGARWQSDEQLHLTLRFIGEVDHHLAEDIAAVLGALHAPAARVRIAGVDLFERQ
GRPHMVWARIAPEEPLAALHRKVEQCLARVGIAAETRAFVPHVTLARLNRSSGPVAPFLALNGDLASDPFLFDHVTLYES
EMGHGGSRYHPVARYPLVDQRAASAEATAATSSHVAAKPSSEP

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 22176; Mature: 22044

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTHRLFVALRPPPPVRALLLRAMHGISGARWQSDEQLHLTLRFIGEVDHHLAEDIAAVL
CCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
GALHAPAARVRIAGVDLFERQGRPHMVWARIAPEEPLAALHRKVEQCLARVGIAAETRAF
HHHCCCHHEEEEECCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHH
VPHVTLARLNRSSGPVAPFLALNGDLASDPFLFDHVTLYESEMGHGGSRYHPVARYPLVD
CCCHHHHHHCCCCCCCCEEEEECCCCCCCCEEEHHHEEHHHHCCCCCCCCCCCCCCCCCC
QRAASAEATAATSSHVAAKPSSEP
CHHCCCHHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
STHRLFVALRPPPPVRALLLRAMHGISGARWQSDEQLHLTLRFIGEVDHHLAEDIAAVL
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
GALHAPAARVRIAGVDLFERQGRPHMVWARIAPEEPLAALHRKVEQCLARVGIAAETRAF
HHHCCCHHEEEEECCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHH
VPHVTLARLNRSSGPVAPFLALNGDLASDPFLFDHVTLYESEMGHGGSRYHPVARYPLVD
CCCHHHHHHCCCCCCCCEEEEECCCCCCCCEEEHHHEEHHHHCCCCCCCCCCCCCCCCCC
QRAASAEATAATSSHVAAKPSSEP
CHHCCCHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]