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The map label for this gene is glyA

Identifier: 103486283

GI number: 103486283

Start: 797053

End: 798360

Strand: Reverse

Name: glyA

Synonym: Sala_0791

Alternate gene names: 103486283

Gene position: 798360-797053 (Counterclockwise)

Preceding gene: 103486284

Following gene: 103486282

Centisome position: 23.87

GC content: 66.44

Gene sequence:

>1308_bases
ATGACCACCGAAACGCTAGACAAGCCCATCAAATCGGCCGGCTATTTCACCGATGGTGTCGCCATCGTCGACCCCGCGGT
CGCGGCGGCGATGACCCGCGAACTCGAACGCGAGCAATATCAGATCGAACTGATTGCGAGCGAGAATATCGTTTCCAGGG
CGGTGCTCGAAGCGCAGGGATCGGTCTTCACCAATAAATATGCCGAAGGCTATCCGGGTCGCCGCTATTATCAGGGCTGC
GCGCCGTCGGACGAGGTCGAACAGCTTGCGATCGACCGCGCGAAACAGTTGTTCGATTGCGGTTATGCGAACGTCCAGCC
GCATTCGGGGGCACAGGCGAACGGCGCGGTGATGCTCGCGCTGACCAAGCCCGGCGCTACGATCCTCGGCATGAGCCTCG
ACGCCGGTGGACACCTGACCCACGGCGCGCCGCCCGCGATGTCGGGCAAATGGTTCAACGCGGTGCAATATGGCGTGCGC
GCCGACGATCATCTCGTCGATTTCGACCAGGTCGAGGCGCTGGCGAGGGAGCATCGCCCTGCCCTCATCATCGCGGGCGG
TTCGGCCTATCCGCGCACGCTCGATTTCGCGCGCTTCCGGGCCATCGCCGACGATGTCGGCGCGTTGCTGATGGTGGACA
TGGCGCATTTCGCGGGGCTCGTCGCGGGCGGCGCGCATCCCTCGCCGATGCAGTACGCGCATGTCGTCACCACGACCACG
CACAAGACGCTGCGCGGCCCGCGCGGCGGCATGATCCTGACCAATGACGAGGCGATCGCCAAGCGCATCAACTCGGCGGT
CTTCCCCGGCCTGCAGGGCGGACCGCTGATGCACGTCATCGCCGCGAAAGCCGTCGCGTTCGGCGAAGCGCTGCGCCCCG
AGTTCAAGGATTATGCAAAAGCCACCATCGCTAACGCGCAGGCGCTCGCGAACCGGCTGAAGGCGCGCGGCGCCGACATC
GTCGCAGGCGGCACCGACACGCATCTGGCGCTGATCGACCTGCGCCCGCTCGGCATCACCGGGCGCGACGCCGACGAAGC
GCTCGAGCGCAGCGCGATCACCTGCAACAAGAACGGCGTGCCCTTCGACCCGCTGCCGCCGGTCAAGACCAGCGGCATCC
GCGTCGGATCGCCCGCGGGCACGACGCGCGGTTTCGGCATCGCCGAGTTCGAGGCGATCGGCGACATGGTCGCCGACGTG
CTCGATGCATTGCGCGACAAGGGCGAGCATGGCGACGCCGATGTCGAGGCCGATGTCCGCGGTCGCGTCCGCGCGCTCTG
CGAACGCTTCCCCATCTATCAGGGTTGA

Upstream 100 bases:

>100_bases
AGGCGTGCCTCGACGCTTTCCTGACCACCGATTTCGCCGGCGACCGCCACGCGCGCCGCGTCGACAAACTCTCTAACCCG
CCGCAACTGGAGACCAGCCG

Downstream 100 bases:

>100_bases
GGGCGATGGCGCGCCAGCAGCCGGAGGAACCCACGCTCGTCGAACTGACGATCGAAGAGGTGAAGGCGATGGGCAAGCAG
GGGATGAAGCACCCCTCGAC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTTETLDKPIKSAGYFTDGVAIVDPAVAAAMTRELEREQYQIELIASENIVSRAVLEAQGSVFTNKYAEGYPGRRYYQGC
APSDEVEQLAIDRAKQLFDCGYANVQPHSGAQANGAVMLALTKPGATILGMSLDAGGHLTHGAPPAMSGKWFNAVQYGVR
ADDHLVDFDQVEALAREHRPALIIAGGSAYPRTLDFARFRAIADDVGALLMVDMAHFAGLVAGGAHPSPMQYAHVVTTTT
HKTLRGPRGGMILTNDEAIAKRINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFKDYAKATIANAQALANRLKARGADI
VAGGTDTHLALIDLRPLGITGRDADEALERSAITCNKNGVPFDPLPPVKTSGIRVGSPAGTTRGFGIAEFEAIGDMVADV
LDALRDKGEHGDADVEADVRGRVRALCERFPIYQG

Sequences:

>Translated_435_residues
MTTETLDKPIKSAGYFTDGVAIVDPAVAAAMTRELEREQYQIELIASENIVSRAVLEAQGSVFTNKYAEGYPGRRYYQGC
APSDEVEQLAIDRAKQLFDCGYANVQPHSGAQANGAVMLALTKPGATILGMSLDAGGHLTHGAPPAMSGKWFNAVQYGVR
ADDHLVDFDQVEALAREHRPALIIAGGSAYPRTLDFARFRAIADDVGALLMVDMAHFAGLVAGGAHPSPMQYAHVVTTTT
HKTLRGPRGGMILTNDEAIAKRINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFKDYAKATIANAQALANRLKARGADI
VAGGTDTHLALIDLRPLGITGRDADEALERSAITCNKNGVPFDPLPPVKTSGIRVGSPAGTTRGFGIAEFEAIGDMVADV
LDALRDKGEHGDADVEADVRGRVRALCERFPIYQG
>Mature_434_residues
TTETLDKPIKSAGYFTDGVAIVDPAVAAAMTRELEREQYQIELIASENIVSRAVLEAQGSVFTNKYAEGYPGRRYYQGCA
PSDEVEQLAIDRAKQLFDCGYANVQPHSGAQANGAVMLALTKPGATILGMSLDAGGHLTHGAPPAMSGKWFNAVQYGVRA
DDHLVDFDQVEALAREHRPALIIAGGSAYPRTLDFARFRAIADDVGALLMVDMAHFAGLVAGGAHPSPMQYAHVVTTTTH
KTLRGPRGGMILTNDEAIAKRINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFKDYAKATIANAQALANRLKARGADIV
AGGTDTHLALIDLRPLGITGRDADEALERSAITCNKNGVPFDPLPPVKTSGIRVGSPAGTTRGFGIAEFEAIGDMVADVL
DALRDKGEHGDADVEADVRGRVRALCERFPIYQG

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=447, Percent_Identity=42.9530201342282, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI261862352, Length=447, Percent_Identity=42.9530201342282, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI261862350, Length=447, Percent_Identity=42.9530201342282, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI261862348, Length=447, Percent_Identity=42.9530201342282, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI22547186, Length=400, Percent_Identity=46.75, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI261862346, Length=447, Percent_Identity=42.2818791946309, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI22547189, Length=383, Percent_Identity=43.8642297650131, Blast_Score=272, Evalue=4e-73,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=55.2884615384615, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25144729, Length=414, Percent_Identity=45.4106280193237, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25144732, Length=414, Percent_Identity=45.4106280193237, Blast_Score=333, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6319739, Length=430, Percent_Identity=40.4651162790698, Blast_Score=309, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6323087, Length=401, Percent_Identity=42.3940149625935, Blast_Score=302, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24640005, Length=392, Percent_Identity=46.1734693877551, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI221329721, Length=392, Percent_Identity=46.1734693877551, Blast_Score=335, Evalue=4e-92,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_SPHAL (Q1GV11)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615844.1
- ProteinModelPortal:   Q1GV11
- SMR:   Q1GV11
- GeneID:   4080435
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0791
- NMPDR:   fig|317655.9.peg.737
- HOGENOM:   HBG301263
- OMA:   DYDQIAA
- ProtClustDB:   PRK00011
- BioCyc:   SALA317655:SALA_0791-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46143; Mature: 46012

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTETLDKPIKSAGYFTDGVAIVDPAVAAAMTRELEREQYQIELIASENIVSRAVLEAQG
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHCC
SVFTNKYAEGYPGRRYYQGCAPSDEVEQLAIDRAKQLFDCGYANVQPHSGAQANGAVMLA
CCHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
LTKPGATILGMSLDAGGHLTHGAPPAMSGKWFNAVQYGVRADDHLVDFDQVEALAREHRP
EECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
ALIIAGGSAYPRTLDFARFRAIADDVGALLMVDMAHFAGLVAGGAHPSPMQYAHVVTTTT
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECC
HKTLRGPRGGMILTNDEAIAKRINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFKDYAK
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHH
ATIANAQALANRLKARGADIVAGGTDTHLALIDLRPLGITGRDADEALERSAITCNKNGV
HHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHCCEEECCCCC
PFDPLPPVKTSGIRVGSPAGTTRGFGIAEFEAIGDMVADVLDALRDKGEHGDADVEADVR
CCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
GRVRALCERFPIYQG
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTETLDKPIKSAGYFTDGVAIVDPAVAAAMTRELEREQYQIELIASENIVSRAVLEAQG
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHCC
SVFTNKYAEGYPGRRYYQGCAPSDEVEQLAIDRAKQLFDCGYANVQPHSGAQANGAVMLA
CCHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
LTKPGATILGMSLDAGGHLTHGAPPAMSGKWFNAVQYGVRADDHLVDFDQVEALAREHRP
EECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
ALIIAGGSAYPRTLDFARFRAIADDVGALLMVDMAHFAGLVAGGAHPSPMQYAHVVTTTT
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECC
HKTLRGPRGGMILTNDEAIAKRINSAVFPGLQGGPLMHVIAAKAVAFGEALRPEFKDYAK
HHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHH
ATIANAQALANRLKARGADIVAGGTDTHLALIDLRPLGITGRDADEALERSAITCNKNGV
HHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHCCEEECCCCC
PFDPLPPVKTSGIRVGSPAGTTRGFGIAEFEAIGDMVADVLDALRDKGEHGDADVEADVR
CCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
GRVRALCERFPIYQG
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA