Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 103486201

GI number: 103486201

Start: 712708

End: 713823

Strand: Direct

Name: dnaN [H]

Synonym: Sala_0708

Alternate gene names: 103486201

Gene position: 712708-713823 (Clockwise)

Preceding gene: 103486199

Following gene: 103486203

Centisome position: 21.31

GC content: 63.71

Gene sequence:

>1116_bases
ATGAAAGCGACGATCGAACGCGCAGTCTTGTTGAAAAGCCTCGGCCACGTTCAATCGGTGGTCGAACGGCGCAATACGAT
CCCGATCCTGTCGAACGTCCTCATCGAAGCCGACGGCACGGGCCAACTCAGGCTGATGGCGACCGACCTTGATCTGCAGG
TGGTCGAAACGATTGCCGCCAAGGTCGAAACGCCGGGTACGACCACCGTATCGGCGCACACCCTGTTCGAGATCGCGCGC
AAGCTGCCCGAAGGATCGGAAGTCAGCCTCGCCGCCGCCGAGGGCAAGGTGCAGGTCAAGGCCGGCCGCTCGAACTTCAA
CCTGCCGACCTTGCCGCGCGACGATTTCCCGGTGATCGCCGAGGGCGACCTGCCGACCAATTTCGAACTGCCCGTCGCCG
AGCTCATCCAGATCATCGACAAGACGCGCTTCGCCATCTCGACCGAGGAAACGCGCTATTATCTGAACGGCATCTTCCTG
CACGTTGCCGAGGATGCGACGGGGCCGGTGCTGAAGGCGGCGGCGACCGACGGCCACCGCCTCGCGCGCTACACGGTGAC
GCGCCCCGACGGTGCGGCCGGGATGCCCGACGTGATCGTTCCGCGCAAATGCGTTGGCGAAATCCGCAAGCTGCTCGACG
AGGCGGAGGGTAATGTCGAAATCAGCCTGTCGGCGAGCAAGATCCGCTTCCAGCTCGGCAACGCGGTCCTGACGTCGAAG
CTGATCGACGGCACCTTCCCCGACTACAGCCGCGTCATTCCGACCGCGAATGACAAGCTGCTGAAAGTCGATCCGAAAAG
CCTGTTCCAGGGCGTCGACCGCGTGTCGACGATCGCCAGCGAAAAGACGCGCGCGGTCAAGGTCGGGCTCGACAAGGACC
GGATCACGCTCAGCGTCACCAGCCCCGAAAACGGCACCGCCGCCGAAGAACTCGCCGCCGCCTACGACAGCGACGCGATG
GAGATCGGCTTCAACGCCCGCTACCTCAGCGACATTTTGGGACAGGTCGATGGCGACAGCGTCGAACTTCACCTCGCCGA
CGCCAACGCCCCTACGCTGATCCGCGAGAGCGAAAAAAGCCCGGCGCTTTACGTGCTGATGCCGATGCGGGTCTGA

Upstream 100 bases:

>100_bases
GGTGGGCATTTTCAGGGGCCGGAATCGGGGCACGAATCGCCTCCGATGCTCTGCCCCCAAGGGACTTCTCCCGCGAAGCA
CACAGGATGGCGGATTAAGA

Downstream 100 bases:

>100_bases
TCGCGTAACAAGCCCGTCATTGCGAACGAAGCGAAGCAATCCAGAGTCGCTTAAACCGCTCTGGATTGCTTCGCTTCGTT
CGCAATGACGAAAATGAAGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKATIERAVLLKSLGHVQSVVERRNTIPILSNVLIEADGTGQLRLMATDLDLQVVETIAAKVETPGTTTVSAHTLFEIAR
KLPEGSEVSLAAAEGKVQVKAGRSNFNLPTLPRDDFPVIAEGDLPTNFELPVAELIQIIDKTRFAISTEETRYYLNGIFL
HVAEDATGPVLKAAATDGHRLARYTVTRPDGAAGMPDVIVPRKCVGEIRKLLDEAEGNVEISLSASKIRFQLGNAVLTSK
LIDGTFPDYSRVIPTANDKLLKVDPKSLFQGVDRVSTIASEKTRAVKVGLDKDRITLSVTSPENGTAAEELAAAYDSDAM
EIGFNARYLSDILGQVDGDSVELHLADANAPTLIRESEKSPALYVLMPMRV

Sequences:

>Translated_371_residues
MKATIERAVLLKSLGHVQSVVERRNTIPILSNVLIEADGTGQLRLMATDLDLQVVETIAAKVETPGTTTVSAHTLFEIAR
KLPEGSEVSLAAAEGKVQVKAGRSNFNLPTLPRDDFPVIAEGDLPTNFELPVAELIQIIDKTRFAISTEETRYYLNGIFL
HVAEDATGPVLKAAATDGHRLARYTVTRPDGAAGMPDVIVPRKCVGEIRKLLDEAEGNVEISLSASKIRFQLGNAVLTSK
LIDGTFPDYSRVIPTANDKLLKVDPKSLFQGVDRVSTIASEKTRAVKVGLDKDRITLSVTSPENGTAAEELAAAYDSDAM
EIGFNARYLSDILGQVDGDSVELHLADANAPTLIRESEKSPALYVLMPMRV
>Mature_371_residues
MKATIERAVLLKSLGHVQSVVERRNTIPILSNVLIEADGTGQLRLMATDLDLQVVETIAAKVETPGTTTVSAHTLFEIAR
KLPEGSEVSLAAAEGKVQVKAGRSNFNLPTLPRDDFPVIAEGDLPTNFELPVAELIQIIDKTRFAISTEETRYYLNGIFL
HVAEDATGPVLKAAATDGHRLARYTVTRPDGAAGMPDVIVPRKCVGEIRKLLDEAEGNVEISLSASKIRFQLGNAVLTSK
LIDGTFPDYSRVIPTANDKLLKVDPKSLFQGVDRVSTIASEKTRAVKVGLDKDRITLSVTSPENGTAAEELAAAYDSDAM
EIGFNARYLSDILGQVDGDSVELHLADANAPTLIRESEKSPALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=37.8666666666667, Blast_Score=250, Evalue=1e-67,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40072; Mature: 40072

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATIERAVLLKSLGHVQSVVERRNTIPILSNVLIEADGTGQLRLMATDLDLQVVETIAA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCHHHHHHHHH
KVETPGTTTVSAHTLFEIARKLPEGSEVSLAAAEGKVQVKAGRSNFNLPTLPRDDFPVIA
HCCCCCCCEEHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEE
EGDLPTNFELPVAELIQIIDKTRFAISTEETRYYLNGIFLHVAEDATGPVLKAAATDGHR
CCCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCCEEEEECCCCCE
LARYTVTRPDGAAGMPDVIVPRKCVGEIRKLLDEAEGNVEISLSASKIRFQLGNAVLTSK
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHEEEEECCCHHHHHH
LIDGTFPDYSRVIPTANDKLLKVDPKSLFQGVDRVSTIASEKTRAVKVGLDKDRITLSVT
HHCCCCCCHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
SPENGTAAEELAAAYDSDAMEIGFNARYLSDILGQVDGDSVELHLADANAPTLIRESEKS
CCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCC
PALYVLMPMRV
CEEEEEEECCC
>Mature Secondary Structure
MKATIERAVLLKSLGHVQSVVERRNTIPILSNVLIEADGTGQLRLMATDLDLQVVETIAA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEEECCCHHHHHHHHH
KVETPGTTTVSAHTLFEIARKLPEGSEVSLAAAEGKVQVKAGRSNFNLPTLPRDDFPVIA
HCCCCCCCEEHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEE
EGDLPTNFELPVAELIQIIDKTRFAISTEETRYYLNGIFLHVAEDATGPVLKAAATDGHR
CCCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCCEEEEECCCCCE
LARYTVTRPDGAAGMPDVIVPRKCVGEIRKLLDEAEGNVEISLSASKIRFQLGNAVLTSK
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHEEEEECCCHHHHHH
LIDGTFPDYSRVIPTANDKLLKVDPKSLFQGVDRVSTIASEKTRAVKVGLDKDRITLSVT
HHCCCCCCHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
SPENGTAAEELAAAYDSDAMEIGFNARYLSDILGQVDGDSVELHLADANAPTLIRESEKS
CCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCC
PALYVLMPMRV
CEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]