Click here to switch to the map view.

The map label for this gene is gloA [H]

Identifier: 103486178

GI number: 103486178

Start: 694744

End: 695181

Strand: Reverse

Name: gloA [H]

Synonym: Sala_0685

Alternate gene names: 103486178

Gene position: 695181-694744 (Counterclockwise)

Preceding gene: 103486179

Following gene: 103486177

Centisome position: 20.78

GC content: 64.61

Gene sequence:

>438_bases
ATGACCAGATATCTTCACACGATGATCCGCGTGTCAGACCCGCAGGCGACGATCGACTTTTTCAGGCTGATCGGGCTGGA
GGAAGTGCGCCGCTTCGACAATGAGGCCGGGCGCTTCACGCTGATCTTTCTCGCCGCGCCCGGACAGGAAGGGGTCGCCG
AGGTCGAGCTGACCTATAACTGGCCGCCCGCGGACGGCAGCGCGCCCGAACACTATAGCGGCGGCCGCAACTTCGGGCAT
CTCGCCTACCGGGTCGACGACATTTACGCGACGTGCCAGCGGCTGATGGACGCCGGAGTGACGATCAACCGTCCGCCGCG
CGACGGACATATGGCGTTCGTGCGCTCACCCGACGGCATTTCGGTCGAACTGCTCCAGGACGGCTATCTCGAACCCGCCG
AACCCTGGGCCTCGATGCCCAACCGGGGAAGCTGGTAG

Upstream 100 bases:

>100_bases
TGTCGAGGAAGCGCAAGGGGCGACAGCGCCAGTGCCCGATGAATTCGTATCTTTGTTCGAAGCGTTTGAAGGTCGGCCAC
TTCGTGCCTAATAGGGGCGC

Downstream 100 bases:

>100_bases
GGCGCAGCGCCACGGGAAGGCGAGACTTTCCGCGGCGACTGTGGCAGAGTGGCGCACCAAGGTCCGCCGGCATCCACTCC
GTCTTGTGGGTGCATGAGCT

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MTRYLHTMIRVSDPQATIDFFRLIGLEEVRRFDNEAGRFTLIFLAAPGQEGVAEVELTYNWPPADGSAPEHYSGGRNFGH
LAYRVDDIYATCQRLMDAGVTINRPPRDGHMAFVRSPDGISVELLQDGYLEPAEPWASMPNRGSW

Sequences:

>Translated_145_residues
MTRYLHTMIRVSDPQATIDFFRLIGLEEVRRFDNEAGRFTLIFLAAPGQEGVAEVELTYNWPPADGSAPEHYSGGRNFGH
LAYRVDDIYATCQRLMDAGVTINRPPRDGHMAFVRSPDGISVELLQDGYLEPAEPWASMPNRGSW
>Mature_144_residues
TRYLHTMIRVSDPQATIDFFRLIGLEEVRRFDNEAGRFTLIFLAAPGQEGVAEVELTYNWPPADGSAPEHYSGGRNFGHL
AYRVDDIYATCQRLMDAGVTINRPPRDGHMAFVRSPDGISVELLQDGYLEPAEPWASMPNRGSW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=32.1678321678322, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787940, Length=131, Percent_Identity=33.587786259542, Blast_Score=80, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323639, Length=145, Percent_Identity=28.2758620689655, Blast_Score=69, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=29.6551724137931, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16331; Mature: 16199

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYLHTMIRVSDPQATIDFFRLIGLEEVRRFDNEAGRFTLIFLAAPGQEGVAEVELTYN
CCCHHEEEEEECCCHHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEE
WPPADGSAPEHYSGGRNFGHLAYRVDDIYATCQRLMDAGVTINRPPRDGHMAFVRSPDGI
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCC
SVELLQDGYLEPAEPWASMPNRGSW
EEEEECCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
TRYLHTMIRVSDPQATIDFFRLIGLEEVRRFDNEAGRFTLIFLAAPGQEGVAEVELTYN
CCHHEEEEEECCCHHHHHHHHHHCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEE
WPPADGSAPEHYSGGRNFGHLAYRVDDIYATCQRLMDAGVTINRPPRDGHMAFVRSPDGI
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCC
SVELLQDGYLEPAEPWASMPNRGSW
EEEEECCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]