Click here to switch to the map view.

The map label for this gene is cobT [H]

Identifier: 103486135

GI number: 103486135

Start: 654582

End: 656405

Strand: Reverse

Name: cobT [H]

Synonym: Sala_0642

Alternate gene names: 103486135

Gene position: 656405-654582 (Counterclockwise)

Preceding gene: 103486136

Following gene: 103486134

Centisome position: 19.62

GC content: 68.26

Gene sequence:

>1824_bases
ATGTCCGCCCAATCCCCCCTCGACGATTTCAAGGCCGCGCTGTCGAGCGTCGCGCGCGCGGTGACGCGCGACGCCGAGGT
CGAGGTGGGCTTTACCGCCGACGCGCCCGCGCAGGTGGGCAAGGCGATCAAGGTGCCGACCCCGTCGCGCACCCTGCCCG
CCGATCAGGTCGCCGAAGCGCGTGGTTTCGCGGACTCCTATGCTTTGCGGATGAAGCATCACAGCGAGAAACTGCACGCC
GCAGCGCGCCCCGCCGATCCCGTTGCCGCCGCCGCCTTCGACGCGATGGAGCGCGCGCGGATCGAGGCGCTGGGCGCGCG
GCATATGGCGGGGATGCGCGGCAATCTGGCCGCCAGCCTCGCGATGCGGATGCGCTCGGACCCGATCAGCCGCGCGCAGG
ACCGGGGCGACGTGCCGATGTCGAGCGCGCTCGAACTGATGCTGCGCGAAGCGCTGACCGGCGAACGCGCGCCGCAGGGG
ACCGAGACCGGCCTGTCGCTGGTGCGCGAATGGATCAGCAAGGAGGCGGGCGGCGATTTGACCGCGCTGTCGATGCTGCT
CGACGATCAGGCCGCGTTCGCCGAAACCGCGAAAATCGCGCTCCGCCACCTCGACCTCATCCAGAGCGACGAGCCGCTGG
AACAAGGCGCCGAGGACGGCGGCGAAGAGGATCAGACCGAAGCCGAAGAAAGTCAGGAAGAGCAGGAAAGCGAAGACGGC
GGTGCCGGCGAAAGCCAGGTCGACGCGCGCGCCGAAATGGCGGGCGATCAGGCCGACGACGGCGACAGCGACCCCGACGC
GCAGGAGATGGAGGCCGACGGCGAACCCGAAATGGGCGGCGAAGGCGACGAGGGGATGCTGCCCGTCCGCCCCAACCGCC
TGCCGGGCGACATTCCCGATTTCAATTACCTCCGCTTCACCGAAAAGCATGACGAGATCATCGCCGCGACCGAGCTGTGC
GACGCCGACGAACTCGCCCGCCTGCGCGCCTATCTCGACCAGCAGATGACGCATCTGCAGGGCGCGGTGACCAAGCTCGC
CAACCGGCTCCAGCGCCGCCTGATGGCGCAGCAGAACCGCGCGTGGGATTTCGATCAGGAGGAGGGCCAGCTCGACGCCG
CGCGGCTCGCGCGCGTCATCGTCTCGCCCGGCCAGTCCTTGAGCTACAAGATCGAGCGTGACACCGACTTCCGCGACACC
GTCGTCTCGCTGCTCATCGACAATAGCGGGTCGATGCGCGGACGCCCGATCAGCATCGCCGCGATCAGCGCCGACATCCT
CGCGCGCACGCTCGAACGCTGCGGCGTCAAGACCGAGATATTGGGCTTCACCACGCGCACATGGAAGGGCGGGCAGAGCC
GCGAGGACTGGCTCGCCGCGGGTCGCCCCGCGCACCCCGGCCGCCTCAACGACCTCCGCCACATCATCTACAAGCCCGCC
GACGAACCCTATCGCCGCGCGCGCAAGTCGCTGGGGCTGATGATGCGCGAGGGGCTGCTCAAGGAAAATATCGACGGCGA
GGCGCTGATGTGGGCGCACAGCCGCATCATCGCCCGCCCCGAGGAACGCCGCATCCTGATGGTGATTTCGGACGGCGCGC
CGGTCGACGACAGCACCTTGTCGGTCAACCACGGCGCCTATCTGGACCAGCACCTGCGCCAGGTCATCGACTGGATCGAA
AACCGCTCGCCCGTCGAACTCTGCGCGATCGGCATCGGCCACGACGTGACGCGCTACTACAGCCGCGCGGTAACGATCAT
GGATGCCGAGCAATTGGGCGGCACGATGGTCGAACAGCTGGCGGGGTTGTTCGACGTGGATTGA

Upstream 100 bases:

>100_bases
ACCGCCATCGACGCCGTGGACCCCGGCTCAGGTCCGGGGTGACGCGATCCGATCTCATCCTCTTCCTTCCGGCATCAAAG
CTGGTTAGAGCCGACTCGAA

Downstream 100 bases:

>100_bases
GCATTGCTTGATGGCAGGATTATCGTAATTTCGTCAGCGTTTACCTTCCCTATGGTTGGGAATTATGTAAACTGTCACCG
TAATGGGTAATGGGGGAACC

Product: cobalt chelatase, CobT subunit

Products: NA

Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobT [H]

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MSAQSPLDDFKAALSSVARAVTRDAEVEVGFTADAPAQVGKAIKVPTPSRTLPADQVAEARGFADSYALRMKHHSEKLHA
AARPADPVAAAAFDAMERARIEALGARHMAGMRGNLAASLAMRMRSDPISRAQDRGDVPMSSALELMLREALTGERAPQG
TETGLSLVREWISKEAGGDLTALSMLLDDQAAFAETAKIALRHLDLIQSDEPLEQGAEDGGEEDQTEAEESQEEQESEDG
GAGESQVDARAEMAGDQADDGDSDPDAQEMEADGEPEMGGEGDEGMLPVRPNRLPGDIPDFNYLRFTEKHDEIIAATELC
DADELARLRAYLDQQMTHLQGAVTKLANRLQRRLMAQQNRAWDFDQEEGQLDAARLARVIVSPGQSLSYKIERDTDFRDT
VVSLLIDNSGSMRGRPISIAAISADILARTLERCGVKTEILGFTTRTWKGGQSREDWLAAGRPAHPGRLNDLRHIIYKPA
DEPYRRARKSLGLMMREGLLKENIDGEALMWAHSRIIARPEERRILMVISDGAPVDDSTLSVNHGAYLDQHLRQVIDWIE
NRSPVELCAIGIGHDVTRYYSRAVTIMDAEQLGGTMVEQLAGLFDVD

Sequences:

>Translated_607_residues
MSAQSPLDDFKAALSSVARAVTRDAEVEVGFTADAPAQVGKAIKVPTPSRTLPADQVAEARGFADSYALRMKHHSEKLHA
AARPADPVAAAAFDAMERARIEALGARHMAGMRGNLAASLAMRMRSDPISRAQDRGDVPMSSALELMLREALTGERAPQG
TETGLSLVREWISKEAGGDLTALSMLLDDQAAFAETAKIALRHLDLIQSDEPLEQGAEDGGEEDQTEAEESQEEQESEDG
GAGESQVDARAEMAGDQADDGDSDPDAQEMEADGEPEMGGEGDEGMLPVRPNRLPGDIPDFNYLRFTEKHDEIIAATELC
DADELARLRAYLDQQMTHLQGAVTKLANRLQRRLMAQQNRAWDFDQEEGQLDAARLARVIVSPGQSLSYKIERDTDFRDT
VVSLLIDNSGSMRGRPISIAAISADILARTLERCGVKTEILGFTTRTWKGGQSREDWLAAGRPAHPGRLNDLRHIIYKPA
DEPYRRARKSLGLMMREGLLKENIDGEALMWAHSRIIARPEERRILMVISDGAPVDDSTLSVNHGAYLDQHLRQVIDWIE
NRSPVELCAIGIGHDVTRYYSRAVTIMDAEQLGGTMVEQLAGLFDVD
>Mature_606_residues
SAQSPLDDFKAALSSVARAVTRDAEVEVGFTADAPAQVGKAIKVPTPSRTLPADQVAEARGFADSYALRMKHHSEKLHAA
ARPADPVAAAAFDAMERARIEALGARHMAGMRGNLAASLAMRMRSDPISRAQDRGDVPMSSALELMLREALTGERAPQGT
ETGLSLVREWISKEAGGDLTALSMLLDDQAAFAETAKIALRHLDLIQSDEPLEQGAEDGGEEDQTEAEESQEEQESEDGG
AGESQVDARAEMAGDQADDGDSDPDAQEMEADGEPEMGGEGDEGMLPVRPNRLPGDIPDFNYLRFTEKHDEIIAATELCD
ADELARLRAYLDQQMTHLQGAVTKLANRLQRRLMAQQNRAWDFDQEEGQLDAARLARVIVSPGQSLSYKIERDTDFRDTV
VSLLIDNSGSMRGRPISIAAISADILARTLERCGVKTEILGFTTRTWKGGQSREDWLAAGRPAHPGRLNDLRHIIYKPAD
EPYRRARKSLGLMMREGLLKENIDGEALMWAHSRIIARPEERRILMVISDGAPVDDSTLSVNHGAYLDQHLRQVIDWIEN
RSPVELCAIGIGHDVTRYYSRAVTIMDAEQLGGTMVEQLAGLFDVD

Specific function: Catalyzes cobalt insertion in the corrin ring [H]

COG id: COG4547

COG function: function code H; Cobalamin biosynthesis protein CobT (nicotinate-mononucleotide:5, 6-dimethylbenzimidazole phosphoribosyltransferase)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006538
- InterPro:   IPR002035 [H]

Pfam domain/function: PF06213 CobT [H]

EC number: =6.6.1.2 [H]

Molecular weight: Translated: 66470; Mature: 66339

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQSPLDDFKAALSSVARAVTRDAEVEVGFTADAPAQVGKAIKVPTPSRTLPADQVAEA
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCEEECCCCCCCCCHHHHHHH
RGFADSYALRMKHHSEKLHAAARPADPVAAAAFDAMERARIEALGARHMAGMRGNLAASL
HCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AMRMRSDPISRAQDRGDVPMSSALELMLREALTGERAPQGTETGLSLVREWISKEAGGDL
HHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCH
TALSMLLDDQAAFAETAKIALRHLDLIQSDEPLEQGAEDGGEEDQTEAEESQEEQESEDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
GAGESQVDARAEMAGDQADDGDSDPDAQEMEADGEPEMGGEGDEGMLPVRPNRLPGDIPD
CCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FNYLRFTEKHDEIIAATELCDADELARLRAYLDQQMTHLQGAVTKLANRLQRRLMAQQNR
CCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AWDFDQEEGQLDAARLARVIVSPGQSLSYKIERDTDFRDTVVSLLIDNSGSMRGRPISIA
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEE
AISADILARTLERCGVKTEILGFTTRTWKGGQSREDWLAAGRPAHPGRLNDLRHIIYKPA
HHHHHHHHHHHHHCCCCCHHHCCEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCC
DEPYRRARKSLGLMMREGLLKENIDGEALMWAHSRIIARPEERRILMVISDGAPVDDSTL
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
SVNHGAYLDQHLRQVIDWIENRSPVELCAIGIGHDVTRYYSRAVTIMDAEQLGGTMVEQL
ECCCCHHHHHHHHHHHHHHCCCCCHHEEEECCCHHHHHHHHHHHEEEEHHHHCHHHHHHH
AGLFDVD
HHHCCCC
>Mature Secondary Structure 
SAQSPLDDFKAALSSVARAVTRDAEVEVGFTADAPAQVGKAIKVPTPSRTLPADQVAEA
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCEEECCCCCCCCCHHHHHHH
RGFADSYALRMKHHSEKLHAAARPADPVAAAAFDAMERARIEALGARHMAGMRGNLAASL
HCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AMRMRSDPISRAQDRGDVPMSSALELMLREALTGERAPQGTETGLSLVREWISKEAGGDL
HHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCH
TALSMLLDDQAAFAETAKIALRHLDLIQSDEPLEQGAEDGGEEDQTEAEESQEEQESEDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
GAGESQVDARAEMAGDQADDGDSDPDAQEMEADGEPEMGGEGDEGMLPVRPNRLPGDIPD
CCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
FNYLRFTEKHDEIIAATELCDADELARLRAYLDQQMTHLQGAVTKLANRLQRRLMAQQNR
CCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AWDFDQEEGQLDAARLARVIVSPGQSLSYKIERDTDFRDTVVSLLIDNSGSMRGRPISIA
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEE
AISADILARTLERCGVKTEILGFTTRTWKGGQSREDWLAAGRPAHPGRLNDLRHIIYKPA
HHHHHHHHHHHHHCCCCCHHHCCEECCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCC
DEPYRRARKSLGLMMREGLLKENIDGEALMWAHSRIIARPEERRILMVISDGAPVDDSTL
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
SVNHGAYLDQHLRQVIDWIENRSPVELCAIGIGHDVTRYYSRAVTIMDAEQLGGTMVEQL
ECCCCHHHHHHHHHHHHHHCCCCCHHEEEECCCHHHHHHHHHHHEEEEHHHHCHHHHHHH
AGLFDVD
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1917840 [H]