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The map label for this gene is alsS [H]

Identifier: 103486132

GI number: 103486132

Start: 651499

End: 653145

Strand: Reverse

Name: alsS [H]

Synonym: Sala_0639

Alternate gene names: 103486132

Gene position: 653145-651499 (Counterclockwise)

Preceding gene: 103486133

Following gene: 103486131

Centisome position: 19.53

GC content: 60.66

Gene sequence:

>1647_bases
ATGAAAAAAGCATCCGATCTGTTCATCGAATGTCTGGAGGAAGAAGGCGTCGAATATATTTTCGGCGTGCCGGGCGAGGA
AAATCTCGATTTCCTCGACAGCCTGTCGCGATCGAAGAAGATCAGGCTGATCCTCACCCGGCACGAACAGGGCGCGGGTT
TCATGGCCGCGACCTATGGCCGCCATACGGGCAAGACCGGCGTCTGCCTTGCGACGCTCGGCCCCGGCGCGACCAACTTC
GTCACCGCCGCCGCCTATGCGCAGCTGGGCGGGATGCCGATGATGATGATCACGGGGCAAAAGCCGATCAAGAAGTCGAA
ACAGGGGCGCTTTCAGATCCTCGACGTCGTCGCGATGATGGGGCCGATCACCAAATATACGCACCAGATGGCGTCGTCGG
ACAATATCCCGAGCCGCGTGCGCGAGGCCTTTCGCCTTGCCGAAGAGGAAAAGCCCGGCGCCGTGCATATCGAGCTGCCC
GAGGACATCGCCGACGAACATACCGATTCGACCCCGTTGAAACGCAGCCATTCGCGGCGACCGAACGCCGACAGCAAATC
GATCCGCGAAGCGGTGAAGGCGCTCGAAAAGGCGAAATCGCCCGTGCTGGTGATCGGTGCCGGCGCGAACCGCACGATGA
CGAGCCGGATGCTGCGGCAATTCATCGAAAAGACCGGCATTCCTTTCCTCACGACGCAGCTCGGCAAAGGCGTGATCGAC
GAGCGACACCCCAGCTTCCTCGGCTGCGCGGCGCTGAGCGCGGGCGATTTCGTCCACCGCGCGGTCGAGGCATCGGACTG
CATCGTCAATCTGGGCCACGACGTGATCGAAAAGCCGCCTTTCTTCATGCAGCGCGACGGCCCGACGGTCATCCATGTGT
CGACCAAGACCGCCGAAGTCGATCCCGTCTATTTTCCCGACATCGAGGTGATCGGCGACATCGCCAATGCGGTGTGGCAG
ATGAAGGAGGACATCGTCGTGAGCGGCGGCTGGAAGTTCGACCATCTGCTCGCCTATCGCAAGGCCGAGGTCGCGCACAC
GGCGCCGCTCGCCAAGGACGAGCGTTTTCCCATCTTTCCGCCCTATCTTGTGCAGCAGATCCGCGACGCGATGCCCGACG
ACGGGATCATCTGTCTCGACAATGGCGTCTATAAAATCTGGTTCGCGCGCGGCTATACCGCGTGCAAGCCGAACACGGTG
CTGCTCGACAATGCGCTCGCGACGATGGGCGCGGGGCTGCCGAGCGCGATGATGTCGGCAATGCTCTATCCCGACCGCAA
GGTCATGGCGATCTGCGGCGACGGCGGCTTCATGATGAACAGCCAGGAGATGGAGACCGCGGTGCGCCTCGGCCTCAATC
TCACCGTGCTCATCCTCAACGACAACAGCTATGGCATGATCCGCTGGAAACAGGCGAATATGGGGTTCAAGGATTGGGGG
CTGACCTATGGCAACCCTGATTTCGTGCGTTATGCCGAAAGCTATGGCGCGAGCGGCCATCGCGTCGAAAGCGCCGCGCA
TCTCAAAGCACTACTCGCCCATTGCCTCGACACGCCGGGGGTCCATCTGATCGACTGTCCGGTCGATTATTCGGAGAATG
ACCAGATATTGAACAACGATATCAAACGGCTGTCGAAGGAGCTTTGA

Upstream 100 bases:

>100_bases
GGCTTATGACGGGCGCCGCTGCAACGATATGATGCCGAGGGGTCGCCGGCATGATCTGGGGCCGCACCATGATGGTGCGC
GGCTTAAGGGGGCGACACGC

Downstream 100 bases:

>100_bases
CCTTTCTCACCCTGCACCCCGGCGGAAGCCGGGGTGCAGAGCGTCCTTGCGCGGCCGTCGCGCGTTCCAGCCCTGGACCC
CGGCTTTCGCCGGGGTACGG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKKASDLFIECLEEEGVEYIFGVPGEENLDFLDSLSRSKKIRLILTRHEQGAGFMAATYGRHTGKTGVCLATLGPGATNF
VTAAAYAQLGGMPMMMITGQKPIKKSKQGRFQILDVVAMMGPITKYTHQMASSDNIPSRVREAFRLAEEEKPGAVHIELP
EDIADEHTDSTPLKRSHSRRPNADSKSIREAVKALEKAKSPVLVIGAGANRTMTSRMLRQFIEKTGIPFLTTQLGKGVID
ERHPSFLGCAALSAGDFVHRAVEASDCIVNLGHDVIEKPPFFMQRDGPTVIHVSTKTAEVDPVYFPDIEVIGDIANAVWQ
MKEDIVVSGGWKFDHLLAYRKAEVAHTAPLAKDERFPIFPPYLVQQIRDAMPDDGIICLDNGVYKIWFARGYTACKPNTV
LLDNALATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDNSYGMIRWKQANMGFKDWG
LTYGNPDFVRYAESYGASGHRVESAAHLKALLAHCLDTPGVHLIDCPVDYSENDQILNNDIKRLSKEL

Sequences:

>Translated_548_residues
MKKASDLFIECLEEEGVEYIFGVPGEENLDFLDSLSRSKKIRLILTRHEQGAGFMAATYGRHTGKTGVCLATLGPGATNF
VTAAAYAQLGGMPMMMITGQKPIKKSKQGRFQILDVVAMMGPITKYTHQMASSDNIPSRVREAFRLAEEEKPGAVHIELP
EDIADEHTDSTPLKRSHSRRPNADSKSIREAVKALEKAKSPVLVIGAGANRTMTSRMLRQFIEKTGIPFLTTQLGKGVID
ERHPSFLGCAALSAGDFVHRAVEASDCIVNLGHDVIEKPPFFMQRDGPTVIHVSTKTAEVDPVYFPDIEVIGDIANAVWQ
MKEDIVVSGGWKFDHLLAYRKAEVAHTAPLAKDERFPIFPPYLVQQIRDAMPDDGIICLDNGVYKIWFARGYTACKPNTV
LLDNALATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDNSYGMIRWKQANMGFKDWG
LTYGNPDFVRYAESYGASGHRVESAAHLKALLAHCLDTPGVHLIDCPVDYSENDQILNNDIKRLSKEL
>Mature_548_residues
MKKASDLFIECLEEEGVEYIFGVPGEENLDFLDSLSRSKKIRLILTRHEQGAGFMAATYGRHTGKTGVCLATLGPGATNF
VTAAAYAQLGGMPMMMITGQKPIKKSKQGRFQILDVVAMMGPITKYTHQMASSDNIPSRVREAFRLAEEEKPGAVHIELP
EDIADEHTDSTPLKRSHSRRPNADSKSIREAVKALEKAKSPVLVIGAGANRTMTSRMLRQFIEKTGIPFLTTQLGKGVID
ERHPSFLGCAALSAGDFVHRAVEASDCIVNLGHDVIEKPPFFMQRDGPTVIHVSTKTAEVDPVYFPDIEVIGDIANAVWQ
MKEDIVVSGGWKFDHLLAYRKAEVAHTAPLAKDERFPIFPPYLVQQIRDAMPDDGIICLDNGVYKIWFARGYTACKPNTV
LLDNALATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDNSYGMIRWKQANMGFKDWG
LTYGNPDFVRYAESYGASGHRVESAAHLKALLAHCLDTPGVHLIDCPVDYSENDQILNNDIKRLSKEL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=25, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI21361361, Length=538, Percent_Identity=25.2788104089219, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790104, Length=536, Percent_Identity=30.9701492537313, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI87081685, Length=550, Percent_Identity=27.6363636363636, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1786717, Length=524, Percent_Identity=26.9083969465649, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1787096, Length=538, Percent_Identity=25.2788104089219, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788716, Length=481, Percent_Identity=26.8191268191268, Blast_Score=128, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17531299, Length=474, Percent_Identity=26.7932489451477, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=474, Percent_Identity=26.7932489451477, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=551, Percent_Identity=23.4119782214156, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=557, Percent_Identity=30.7001795332136, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6320816, Length=480, Percent_Identity=25.8333333333333, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=485, Percent_Identity=22.8865979381443, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=482, Percent_Identity=23.6514522821577, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323073, Length=481, Percent_Identity=22.6611226611227, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=28.1573498964803, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60385; Mature: 60385

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKASDLFIECLEEEGVEYIFGVPGEENLDFLDSLSRSKKIRLILTRHEQGAGFMAATYG
CCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHCCCCEEEEEEEECCCCCCEEEEECC
RHTGKTGVCLATLGPGATNFVTAAAYAQLGGMPMMMITGQKPIKKSKQGRFQILDVVAMM
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCHHHHHHHHHH
GPITKYTHQMASSDNIPSRVREAFRLAEEEKPGAVHIELPEDIADEHTDSTPLKRSHSRR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCHHHCCCC
PNADSKSIREAVKALEKAKSPVLVIGAGANRTMTSRMLRQFIEKTGIPFLTTQLGKGVID
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEHHHCCCCCC
ERHPSFLGCAALSAGDFVHRAVEASDCIVNLGHDVIEKPPFFMQRDGPTVIHVSTKTAEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCCCCCEEECCCCEEEEEECCCCCC
DPVYFPDIEVIGDIANAVWQMKEDIVVSGGWKFDHLLAYRKAEVAHTAPLAKDERFPIFP
CCEECCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PYLVQQIRDAMPDDGIICLDNGVYKIWFARGYTACKPNTVLLDNALATMGAGLPSAMMSA
HHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCCCCCCEEEECCHHHHHCCCCHHHHHHH
MLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDNSYGMIRWKQANMGFKDWG
HHCCCCEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCCCHHCC
LTYGNPDFVRYAESYGASGHRVESAAHLKALLAHCLDTPGVHLIDCPVDYSENDQILNND
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHH
IKRLSKEL
HHHHHHCC
>Mature Secondary Structure
MKKASDLFIECLEEEGVEYIFGVPGEENLDFLDSLSRSKKIRLILTRHEQGAGFMAATYG
CCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHCCCCEEEEEEEECCCCCCEEEEECC
RHTGKTGVCLATLGPGATNFVTAAAYAQLGGMPMMMITGQKPIKKSKQGRFQILDVVAMM
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCHHHHHHHHHH
GPITKYTHQMASSDNIPSRVREAFRLAEEEKPGAVHIELPEDIADEHTDSTPLKRSHSRR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCHHHCCCC
PNADSKSIREAVKALEKAKSPVLVIGAGANRTMTSRMLRQFIEKTGIPFLTTQLGKGVID
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEHHHCCCCCC
ERHPSFLGCAALSAGDFVHRAVEASDCIVNLGHDVIEKPPFFMQRDGPTVIHVSTKTAEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCCCCCEEECCCCEEEEEECCCCCC
DPVYFPDIEVIGDIANAVWQMKEDIVVSGGWKFDHLLAYRKAEVAHTAPLAKDERFPIFP
CCEECCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PYLVQQIRDAMPDDGIICLDNGVYKIWFARGYTACKPNTVLLDNALATMGAGLPSAMMSA
HHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCCCCCCEEEECCHHHHHCCCCHHHHHHH
MLYPDRKVMAICGDGGFMMNSQEMETAVRLGLNLTVLILNDNSYGMIRWKQANMGFKDWG
HHCCCCEEEEEECCCCEEECHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCCCHHCC
LTYGNPDFVRYAESYGASGHRVESAAHLKALLAHCLDTPGVHLIDCPVDYSENDQILNND
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHH
IKRLSKEL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]