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The map label for this gene is norM [H]

Identifier: 103485947

GI number: 103485947

Start: 465182

End: 466615

Strand: Reverse

Name: norM [H]

Synonym: Sala_0454

Alternate gene names: 103485947

Gene position: 466615-465182 (Counterclockwise)

Preceding gene: 103485950

Following gene: 103485946

Centisome position: 13.95

GC content: 67.64

Gene sequence:

>1434_bases
ATGCTGCACCAGCCGACCTCCGCCGCCGCGCATCGTGCGCAGGGATTTCGCGACGAGTTTCGCGCGACGCTGGCGCTTGC
CTGGCCGCTGGTGCTCACCAACCTGACGATGTCGCTGATCGGCGCGACCGACGTGCTGATGGTCGGGCGGCTGGGGCCGA
CCGAGCTCGCGGCCTCGTCGCTCGGCTTCAACCTTGCGATGCTGCTCGGCATCTTCGGCATGGGGCTGGTGATGGGGGCA
TCGCCGCTGATGGCGAGCGAGATCGGGCGCCGCGCCAATTCGGTGCGCGACATCCGGCGCACCTTTCGTCAGACGCTGTG
GCTCGTCGCCATCGTCGCGGTGCCGATGCTCGCCATCCTCTGGAACACCGGCGCGATCCTGGACCTGCTCGGACAGGACC
GGGCGCTGGCGGCGCTCGCGCAGGATTATGTGCGCGCCTATATGTGGTCGATCCCGCTGTTCCTTGCGACGCTGGCGTTC
CGCAACTTCCTTGCGGCGCTCGAACGGCCGATGTGGTCGCTGGTCGTCGGGGTCGTCGGGGTGCTCGGCAATATCCTGTT
CAACTATGCGCTGATCTTCGGCAAGTTCGGGATGCCCGCGCTCGGCATCGTCGGCGCGGGTGTCGGCAGCGTGCTGACCA
ATATGGTGATGCTGCTGCTGATGGTCGGCGTCGTATATCGCGACCGGCAGTTCCGCCGCTATCACCTGCTCGGTCGCTGG
TGGCGCAGCGACTGGCCGCGCTTTGCCCAGATGACGCGCATCGGCACCCCCATCGCCGTCAGCCATGCGTTCGAGGCCGG
GGTCTTTTCGGCGGCGGTCATGCTGATGGGCTGGATCTCGACCGCCGCGGTCGCGGCGCACGCGGTCGCGCTGCAACTTG
CCTCGCTGACCTTCATGGTGCCGATGGGGCTCGCGCAGGCGGCGACGGTGCGCGTCGGCATCGGCCATGGCCGCGCCGAT
GCCCATCATATCCGCCGTGCGGGCTGGACGAGTTTCGCCATGGGCACGGGTTTCATGGCGGCGATGGCGATCATCATGCT
GGCAATCCCCGGAACGCTCGCCGGCCTGTTCATCGACCGCACCAACCCCGCCAATGCCGAAGTCGCCGAACTGGCGGTCA
GCTTCCTGGTCATCGCGGCGCTTTTTCAGGTCGCCGATGGCGCGCAGGTGGTGGCGCAGGGGATGCTGCGCGGCCTGCAC
GACACCTTTGTCCCGATGCTGTTCGCGCTTTTCGGTTACTGGGTGATCGGCATCGGTGTCGGCGCCTGGCTGGCGTTCGA
GCGCGGCTGGGGCGGGGTGGGCATATGGACCGGCCTTGCCACCGGCCTCTCCATTGTGGCGGTGTTGATGCTCACCCGCT
GGATGCAGCGCGAACGGCTGGGGCTGGTGCGTCAGACGGCACCCTTGCGCGAGAGTGTTGCAGCCCCCATCTGA

Upstream 100 bases:

>100_bases
GAACAGTCTGTTGCCTTTCTGCCACCCCTTCCCGACAATCCGCTCCCGCGCGCCGGGCCGCACTTGAACGGTCACATTCG
CCCGCCTATCCGGGCCGGCT

Downstream 100 bases:

>100_bases
TTTGCATCGCCGCGCGCCGAAAATTTTCGCACAGTTGACTTGACGCTGTGACACCCCCCGCCCATATGCCCGCCTGTTAG
CACTCTCCTGTGGCGAGTGC

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLHQPTSAAAHRAQGFRDEFRATLALAWPLVLTNLTMSLIGATDVLMVGRLGPTELAASSLGFNLAMLLGIFGMGLVMGA
SPLMASEIGRRANSVRDIRRTFRQTLWLVAIVAVPMLAILWNTGAILDLLGQDRALAALAQDYVRAYMWSIPLFLATLAF
RNFLAALERPMWSLVVGVVGVLGNILFNYALIFGKFGMPALGIVGAGVGSVLTNMVMLLLMVGVVYRDRQFRRYHLLGRW
WRSDWPRFAQMTRIGTPIAVSHAFEAGVFSAAVMLMGWISTAAVAAHAVALQLASLTFMVPMGLAQAATVRVGIGHGRAD
AHHIRRAGWTSFAMGTGFMAAMAIIMLAIPGTLAGLFIDRTNPANAEVAELAVSFLVIAALFQVADGAQVVAQGMLRGLH
DTFVPMLFALFGYWVIGIGVGAWLAFERGWGGVGIWTGLATGLSIVAVLMLTRWMQRERLGLVRQTAPLRESVAAPI

Sequences:

>Translated_477_residues
MLHQPTSAAAHRAQGFRDEFRATLALAWPLVLTNLTMSLIGATDVLMVGRLGPTELAASSLGFNLAMLLGIFGMGLVMGA
SPLMASEIGRRANSVRDIRRTFRQTLWLVAIVAVPMLAILWNTGAILDLLGQDRALAALAQDYVRAYMWSIPLFLATLAF
RNFLAALERPMWSLVVGVVGVLGNILFNYALIFGKFGMPALGIVGAGVGSVLTNMVMLLLMVGVVYRDRQFRRYHLLGRW
WRSDWPRFAQMTRIGTPIAVSHAFEAGVFSAAVMLMGWISTAAVAAHAVALQLASLTFMVPMGLAQAATVRVGIGHGRAD
AHHIRRAGWTSFAMGTGFMAAMAIIMLAIPGTLAGLFIDRTNPANAEVAELAVSFLVIAALFQVADGAQVVAQGMLRGLH
DTFVPMLFALFGYWVIGIGVGAWLAFERGWGGVGIWTGLATGLSIVAVLMLTRWMQRERLGLVRQTAPLRESVAAPI
>Mature_477_residues
MLHQPTSAAAHRAQGFRDEFRATLALAWPLVLTNLTMSLIGATDVLMVGRLGPTELAASSLGFNLAMLLGIFGMGLVMGA
SPLMASEIGRRANSVRDIRRTFRQTLWLVAIVAVPMLAILWNTGAILDLLGQDRALAALAQDYVRAYMWSIPLFLATLAF
RNFLAALERPMWSLVVGVVGVLGNILFNYALIFGKFGMPALGIVGAGVGSVLTNMVMLLLMVGVVYRDRQFRRYHLLGRW
WRSDWPRFAQMTRIGTPIAVSHAFEAGVFSAAVMLMGWISTAAVAAHAVALQLASLTFMVPMGLAQAATVRVGIGHGRAD
AHHIRRAGWTSFAMGTGFMAAMAIIMLAIPGTLAGLFIDRTNPANAEVAELAVSFLVIAALFQVADGAQVVAQGMLRGLH
DTFVPMLFALFGYWVIGIGVGAWLAFERGWGGVGIWTGLATGLSIVAVLMLTRWMQRERLGLVRQTAPLRESVAAPI

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=446, Percent_Identity=25.5605381165919, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI153792564, Length=431, Percent_Identity=25.0580046403712, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI38261960, Length=466, Percent_Identity=23.8197424892704, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI48994890, Length=463, Percent_Identity=32.6133909287257, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI226510954, Length=446, Percent_Identity=24.8878923766816, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320545, Length=446, Percent_Identity=21.52466367713, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 51389; Mature: 51389

Theoretical pI: Translated: 11.76; Mature: 11.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHQPTSAAAHRAQGFRDEFRATLALAWPLVLTNLTMSLIGATDVLMVGRLGPTELAASS
CCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LGFNLAMLLGIFGMGLVMGASPLMASEIGRRANSVRDIRRTFRQTLWLVAIVAVPMLAIL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WNTGAILDLLGQDRALAALAQDYVRAYMWSIPLFLATLAFRNFLAALERPMWSLVVGVVG
HCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VLGNILFNYALIFGKFGMPALGIVGAGVGSVLTNMVMLLLMVGVVYRDRQFRRYHLLGRW
HHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRSDWPRFAQMTRIGTPIAVSHAFEAGVFSAAVMLMGWISTAAVAAHAVALQLASLTFMV
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMGLAQAATVRVGIGHGRADAHHIRRAGWTSFAMGTGFMAAMAIIMLAIPGTLAGLFIDR
HHHHHHHHHEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
TNPANAEVAELAVSFLVIAALFQVADGAQVVAQGMLRGLHDTFVPMLFALFGYWVIGIGV
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAWLAFERGWGGVGIWTGLATGLSIVAVLMLTRWMQRERLGLVRQTAPLRESVAAPI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure
MLHQPTSAAAHRAQGFRDEFRATLALAWPLVLTNLTMSLIGATDVLMVGRLGPTELAASS
CCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LGFNLAMLLGIFGMGLVMGASPLMASEIGRRANSVRDIRRTFRQTLWLVAIVAVPMLAIL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WNTGAILDLLGQDRALAALAQDYVRAYMWSIPLFLATLAFRNFLAALERPMWSLVVGVVG
HCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VLGNILFNYALIFGKFGMPALGIVGAGVGSVLTNMVMLLLMVGVVYRDRQFRRYHLLGRW
HHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRSDWPRFAQMTRIGTPIAVSHAFEAGVFSAAVMLMGWISTAAVAAHAVALQLASLTFMV
HHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMGLAQAATVRVGIGHGRADAHHIRRAGWTSFAMGTGFMAAMAIIMLAIPGTLAGLFIDR
HHHHHHHHHEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
TNPANAEVAELAVSFLVIAALFQVADGAQVVAQGMLRGLHDTFVPMLFALFGYWVIGIGV
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAWLAFERGWGGVGIWTGLATGLSIVAVLMLTRWMQRERLGLVRQTAPLRESVAAPI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]