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The map label for this gene is clpB [H]

Identifier: 103485900

GI number: 103485900

Start: 415330

End: 417918

Strand: Reverse

Name: clpB [H]

Synonym: Sala_0406

Alternate gene names: 103485900

Gene position: 417918-415330 (Counterclockwise)

Preceding gene: 103485901

Following gene: 103485899

Centisome position: 12.49

GC content: 61.03

Gene sequence:

>2589_bases
ATGAACCTCGAGAAATTCACCGACCGGGCCAAGGGCTTTCTACAGAGCGCCCAGACAGTTGCCGTCCGCATGAGCCATCA
GCGCATCACGCCAGTCCATATTCTCAAGGCATTGCTCGAAGATGAGGAGGGCATGGCCGCGCAGCTCATCCAGCGAGCAG
GCGGTAGCCCCCCTGCCGCCATTGGCGAGGTTGAAAAGGCATTGCATAAATTCCCGGCGGTTTCCGGCTCCGGAGCGCAG
ACGCCGCCCGCCCTAGACAATGATTCGGCTCGCCTGCTCGATCAGGCCGAACAGCTGGCAAAGAAGGCCGGCGACAGCTT
CGTTCCTGTACAGCGCATATTGCAGGCCATGGCCCTTTCCGACAGCACCGACGCAGGCAAGGCTTTGCAGGCTGCCGGCG
TAAATGCGAAGTCGCTCGAGGCGGTGATTCAGGAAGTGACCGGCGGTCGCACCGCGGACAGCGCAAGCGCAGAAGAGAGC
TACGATGCGCTCAAGAAATACGCCCGCGACCTGACCCAAGCGGCGCGCGACGGCAAGCTCGACCCGGTGATCGGCCGCGA
CGAGGAGATTCGTCGCACAATCCAGATTCTGGCCCGCAGGACCAAGAATAATCCCGTTCTTATCGGTGATCCCGGCGTGG
GCAAGACCGCGATCGCCGAGGGGCTGGCGCTGCGCATCGCCAATGGCGATGTGCCCGACAGCCTCAAGGGCCGCACGCTA
ATGGCGCTTGACATGGGCGCGCTGATTGCGGGAGCGAAATATCGCGGCGAATTCGAGGAACGCTTGAAGGCCGTGATCGA
CGAGGTGAAAAATTCCGATGGGCAGATCATCCTGTTCATCGACGAGATGCACACGCTGATTGGAGCGGGCGCGAGCGAGG
GCAGCATGGATGCCTCGAACCTCCTGAAGCCTGCGCTGTCGCGCGGCGAGTTGCACGTGATCGGCGCGACTACGCTCGAC
GAATACCAGAAATATGTCGAGAAGGATGCCGCACTCCAGAGGCGCTTCCAGCCGGTCTATATCGACGAGCCGAGCGTAGA
GGACACGATATCGATCCTGCGTGGCCTGAAGGAAAAATACGAACTGCACCACGGGGTAAACATTACCGACAGCGCGATCG
TGGCCGCTGCGCAGCTATCCGATCGCTATATCCAGAACCGCTTCCTCCCCGACAAGGCGATCGACCTGATGGACGAGGCG
GCGAGTCGCATCCGCATGGAAGTAGAAAGCAAACCCGAAGAGATCGAAAACCTCGACCGCCGCATTATCCAGCTCAAGAT
CGAGGAAAGCGCGCTTGCTAAGGAAAGCGACGAGGCGAGCAAGGACCGGCTCGCCACCTTGCGCAAAGAATTGGCCGAGC
TCGAGCAGAAATCGAGCGAGCTGACCACGCGCTGGCAGAACGAACGCGACAAGATTCAGGCTGAGGCAAAGATCAAGGAA
CAGCTCGACCTGGCCCGGCTCGAACTGGAGCAGGCGCAGCGCGCGGGCGACCTGCAAAAGGCGGGCGAGTTGTCTTATGG
CACGATACCGAGCCTCGAAAAGCAGCTTGAAGAGGCACGTGGTGCAAATGAGACTGCGTTATTGCGCGAGGAAGTGACCG
AGGAGGACATCGCCTCTGTCGTTAGCCGCTGGACAGGCGTGCCGGTGGACAAGATGATGGAGGGTGAGCGCGAGAAGCTG
CTCAAGATGGAAGAGTTTCTCGGTCGCCGCGTGATCGGCCAGCAGCAGGCGGTACAGGCTGTCTCCAAGGCCGTGCGCCG
AGCACGCGCAGGCTTGCAGGATCCGAACCGGCCATTAGGCAGCTTCCTGTTCCTTGGCCCCACGGGCGTTGGCAAAACCG
AGCTGACCAAGGCTCTGGCCGAGTTCATGTTCGGCGACGAGCGCGCCATGGTGCGCATCGACATGAGCGAATTCATGGAA
AAGCACGCGGTCGCCAGACTGATCGGCGCGCCTCCGGGTTATGTTGGGTACGAGGAAGGCGGCGTGCTTACCGAGGCGGT
GCGGCGGCGACCTTACCAGGTGGTGCTGTTCGACGAAGTTGAGAAGGCGCATCAGGACGTGTTCAACGTTCTGCTGCAGG
TACTCGACGACGGTCGCCTGACCGACGGGCAGGGCCGGGTGGTGGACTTCTCGAACACGCTGATCATCTTGACCAGCAAC
CTCGGCAGCCAGTTCCTCTCCAACCTCACCGACGATCAGAAGGTGGAAGACGTCGAGCCGCAGGTGATGGACGTGGTGCG
CAGCCACTTCCGCCCCGAGTTCCTCAACCGGCTTGATGAAATCATTCTGTTCCATCGCCTGAGCCAGGAGCACATGGCAC
CGATCGTCGATATCCAGGTCAAGCGCGTCCAGAAGCTGCTGAAGGACCGGAAGATCACGCTCGACCTCACCGAAGGCGCG
CGCAACTGGCTCGGTCGGGTGGGTTACGACCCGGTATATGGCGCCAGACCGCTCAAGCGAGCGGTACAGCGCTATCTGCA
GGATCCGCTGGCGGAAAAGCTGCTGGCCGGCGAAATCCTTGACGGTTCGACCGTCAAGGTCGACGAGGGCGAAGAAGGTC
TCGTTTTCGTCATTCCATCCCAGATCTGA

Upstream 100 bases:

>100_bases
CGTTGCGGGTTGATATTTGAGCAGACGGGAGTTCGATGTCCTGCTAGTACCTACTCAAAGTCCCATTTGAAATTGTAATT
ACGCTTGGAGACCCTTTGCG

Downstream 100 bases:

>100_bases
GCAGCGCGAGCGCACGACAAGCAAGGGAAGGGCGGCGAATGGAAGCCGACAAGATCAAGGTAGTTTGCCCACATTGTGAT
GCGGTCAACGCTATCCCGCC

Product: ATPase AAA-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 862; Mature: 862

Protein sequence:

>862_residues
MNLEKFTDRAKGFLQSAQTVAVRMSHQRITPVHILKALLEDEEGMAAQLIQRAGGSPPAAIGEVEKALHKFPAVSGSGAQ
TPPALDNDSARLLDQAEQLAKKAGDSFVPVQRILQAMALSDSTDAGKALQAAGVNAKSLEAVIQEVTGGRTADSASAEES
YDALKKYARDLTQAARDGKLDPVIGRDEEIRRTIQILARRTKNNPVLIGDPGVGKTAIAEGLALRIANGDVPDSLKGRTL
MALDMGALIAGAKYRGEFEERLKAVIDEVKNSDGQIILFIDEMHTLIGAGASEGSMDASNLLKPALSRGELHVIGATTLD
EYQKYVEKDAALQRRFQPVYIDEPSVEDTISILRGLKEKYELHHGVNITDSAIVAAAQLSDRYIQNRFLPDKAIDLMDEA
ASRIRMEVESKPEEIENLDRRIIQLKIEESALAKESDEASKDRLATLRKELAELEQKSSELTTRWQNERDKIQAEAKIKE
QLDLARLELEQAQRAGDLQKAGELSYGTIPSLEKQLEEARGANETALLREEVTEEDIASVVSRWTGVPVDKMMEGEREKL
LKMEEFLGRRVIGQQQAVQAVSKAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELTKALAEFMFGDERAMVRIDMSEFME
KHAVARLIGAPPGYVGYEEGGVLTEAVRRRPYQVVLFDEVEKAHQDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
LGSQFLSNLTDDQKVEDVEPQVMDVVRSHFRPEFLNRLDEIILFHRLSQEHMAPIVDIQVKRVQKLLKDRKITLDLTEGA
RNWLGRVGYDPVYGARPLKRAVQRYLQDPLAEKLLAGEILDGSTVKVDEGEEGLVFVIPSQI

Sequences:

>Translated_862_residues
MNLEKFTDRAKGFLQSAQTVAVRMSHQRITPVHILKALLEDEEGMAAQLIQRAGGSPPAAIGEVEKALHKFPAVSGSGAQ
TPPALDNDSARLLDQAEQLAKKAGDSFVPVQRILQAMALSDSTDAGKALQAAGVNAKSLEAVIQEVTGGRTADSASAEES
YDALKKYARDLTQAARDGKLDPVIGRDEEIRRTIQILARRTKNNPVLIGDPGVGKTAIAEGLALRIANGDVPDSLKGRTL
MALDMGALIAGAKYRGEFEERLKAVIDEVKNSDGQIILFIDEMHTLIGAGASEGSMDASNLLKPALSRGELHVIGATTLD
EYQKYVEKDAALQRRFQPVYIDEPSVEDTISILRGLKEKYELHHGVNITDSAIVAAAQLSDRYIQNRFLPDKAIDLMDEA
ASRIRMEVESKPEEIENLDRRIIQLKIEESALAKESDEASKDRLATLRKELAELEQKSSELTTRWQNERDKIQAEAKIKE
QLDLARLELEQAQRAGDLQKAGELSYGTIPSLEKQLEEARGANETALLREEVTEEDIASVVSRWTGVPVDKMMEGEREKL
LKMEEFLGRRVIGQQQAVQAVSKAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELTKALAEFMFGDERAMVRIDMSEFME
KHAVARLIGAPPGYVGYEEGGVLTEAVRRRPYQVVLFDEVEKAHQDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
LGSQFLSNLTDDQKVEDVEPQVMDVVRSHFRPEFLNRLDEIILFHRLSQEHMAPIVDIQVKRVQKLLKDRKITLDLTEGA
RNWLGRVGYDPVYGARPLKRAVQRYLQDPLAEKLLAGEILDGSTVKVDEGEEGLVFVIPSQI
>Mature_862_residues
MNLEKFTDRAKGFLQSAQTVAVRMSHQRITPVHILKALLEDEEGMAAQLIQRAGGSPPAAIGEVEKALHKFPAVSGSGAQ
TPPALDNDSARLLDQAEQLAKKAGDSFVPVQRILQAMALSDSTDAGKALQAAGVNAKSLEAVIQEVTGGRTADSASAEES
YDALKKYARDLTQAARDGKLDPVIGRDEEIRRTIQILARRTKNNPVLIGDPGVGKTAIAEGLALRIANGDVPDSLKGRTL
MALDMGALIAGAKYRGEFEERLKAVIDEVKNSDGQIILFIDEMHTLIGAGASEGSMDASNLLKPALSRGELHVIGATTLD
EYQKYVEKDAALQRRFQPVYIDEPSVEDTISILRGLKEKYELHHGVNITDSAIVAAAQLSDRYIQNRFLPDKAIDLMDEA
ASRIRMEVESKPEEIENLDRRIIQLKIEESALAKESDEASKDRLATLRKELAELEQKSSELTTRWQNERDKIQAEAKIKE
QLDLARLELEQAQRAGDLQKAGELSYGTIPSLEKQLEEARGANETALLREEVTEEDIASVVSRWTGVPVDKMMEGEREKL
LKMEEFLGRRVIGQQQAVQAVSKAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELTKALAEFMFGDERAMVRIDMSEFME
KHAVARLIGAPPGYVGYEEGGVLTEAVRRRPYQVVLFDEVEKAHQDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
LGSQFLSNLTDDQKVEDVEPQVMDVVRSHFRPEFLNRLDEIILFHRLSQEHMAPIVDIQVKRVQKLLKDRKITLDLTEGA
RNWLGRVGYDPVYGARPLKRAVQRYLQDPLAEKLLAGEILDGSTVKVDEGEEGLVFVIPSQI

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=361, Percent_Identity=34.9030470914127, Blast_Score=203, Evalue=6e-52,
Organism=Escherichia coli, GI1788943, Length=859, Percent_Identity=57.3923166472643, Blast_Score=998, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=319, Percent_Identity=44.5141065830721, Blast_Score=283, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=706, Percent_Identity=55.2407932011331, Blast_Score=780, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=861, Percent_Identity=43.4378629500581, Blast_Score=685, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: 3.4.21.92

Molecular weight: Translated: 95304; Mature: 95304

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKFTDRAKGFLQSAQTVAVRMSHQRITPVHILKALLEDEEGMAAQLIQRAGGSPPAA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
IGEVEKALHKFPAVSGSGAQTPPALDNDSARLLDQAEQLAKKAGDSFVPVQRILQAMALS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DSTDAGKALQAAGVNAKSLEAVIQEVTGGRTADSASAEESYDALKKYARDLTQAARDGKL
CCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DPVIGRDEEIRRTIQILARRTKNNPVLIGDPGVGKTAIAEGLALRIANGDVPDSLKGRTL
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCEE
MALDMGALIAGAKYRGEFEERLKAVIDEVKNSDGQIILFIDEMHTLIGAGASEGSMDASN
EEEHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCCCCCCHHH
LLKPALSRGELHVIGATTLDEYQKYVEKDAALQRRFQPVYIDEPSVEDTISILRGLKEKY
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHH
ELHHGVNITDSAIVAAAQLSDRYIQNRFLPDKAIDLMDEAASRIRMEVESKPEEIENLDR
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RIIQLKIEESALAKESDEASKDRLATLRKELAELEQKSSELTTRWQNERDKIQAEAKIKE
HHHHEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QLDLARLELEQAQRAGDLQKAGELSYGTIPSLEKQLEEARGANETALLREEVTEEDIASV
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VSRWTGVPVDKMMEGEREKLLKMEEFLGRRVIGQQQAVQAVSKAVRRARAGLQDPNRPLG
HHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SFLFLGPTGVGKTELTKALAEFMFGDERAMVRIDMSEFMEKHAVARLIGAPPGYVGYEEG
CEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GVLTEAVRRRPYQVVLFDEVEKAHQDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
CHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECC
LGSQFLSNLTDDQKVEDVEPQVMDVVRSHFRPEFLNRLDEIILFHRLSQEHMAPIVDIQV
HHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KRVQKLLKDRKITLDLTEGARNWLGRVGYDPVYGARPLKRAVQRYLQDPLAEKLLAGEIL
HHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCC
DGSTVKVDEGEEGLVFVIPSQI
CCCEEEECCCCCCEEEEECCCC
>Mature Secondary Structure
MNLEKFTDRAKGFLQSAQTVAVRMSHQRITPVHILKALLEDEEGMAAQLIQRAGGSPPAA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
IGEVEKALHKFPAVSGSGAQTPPALDNDSARLLDQAEQLAKKAGDSFVPVQRILQAMALS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
DSTDAGKALQAAGVNAKSLEAVIQEVTGGRTADSASAEESYDALKKYARDLTQAARDGKL
CCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DPVIGRDEEIRRTIQILARRTKNNPVLIGDPGVGKTAIAEGLALRIANGDVPDSLKGRTL
CCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCCCCCCEE
MALDMGALIAGAKYRGEFEERLKAVIDEVKNSDGQIILFIDEMHTLIGAGASEGSMDASN
EEEHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCCCCCCHHH
LLKPALSRGELHVIGATTLDEYQKYVEKDAALQRRFQPVYIDEPSVEDTISILRGLKEKY
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHH
ELHHGVNITDSAIVAAAQLSDRYIQNRFLPDKAIDLMDEAASRIRMEVESKPEEIENLDR
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RIIQLKIEESALAKESDEASKDRLATLRKELAELEQKSSELTTRWQNERDKIQAEAKIKE
HHHHEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QLDLARLELEQAQRAGDLQKAGELSYGTIPSLEKQLEEARGANETALLREEVTEEDIASV
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VSRWTGVPVDKMMEGEREKLLKMEEFLGRRVIGQQQAVQAVSKAVRRARAGLQDPNRPLG
HHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SFLFLGPTGVGKTELTKALAEFMFGDERAMVRIDMSEFMEKHAVARLIGAPPGYVGYEEG
CEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GVLTEAVRRRPYQVVLFDEVEKAHQDVFNVLLQVLDDGRLTDGQGRVVDFSNTLIILTSN
CHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECC
LGSQFLSNLTDDQKVEDVEPQVMDVVRSHFRPEFLNRLDEIILFHRLSQEHMAPIVDIQV
HHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KRVQKLLKDRKITLDLTEGARNWLGRVGYDPVYGARPLKRAVQRYLQDPLAEKLLAGEIL
HHHHHHHHCCCEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCC
DGSTVKVDEGEEGLVFVIPSQI
CCCEEEECCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]