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The map label for this gene is tolB

Identifier: 103485823

GI number: 103485823

Start: 333811

End: 335175

Strand: Reverse

Name: tolB

Synonym: Sala_0328

Alternate gene names: 103485823

Gene position: 335175-333811 (Counterclockwise)

Preceding gene: 103485824

Following gene: 103485822

Centisome position: 10.02

GC content: 67.03

Gene sequence:

>1365_bases
ATGTCCCTGCGTCCGATCAGCCTAATGCTCGCCCTGCTGCTGACCAGCGCACCAGCGCTCGCCCAGTCCGCCCCGACGCC
GCCCGCGACAGTGCAGACCGAACCGCGCGAGACGATCGAGGGCACGCTGTCGCAGGAACGCACGGTCATCGGCATCCCGT
CCTTCGCCACCGCCTCGGTCCAGACCGTCGCGGGGCTGCGCACCGACGCCCTCGGCCGCCAGCTTGCCGACGTCATCGCC
GCCGACCTGGAACGCAGCGGCCTCTTTGAACCGATCGGCCCGAACGGCGTCCGCGCGATCGGCCGCGCCGAGGTGCAGGC
GCCGCGCTTTGGCGAATGGCAAGCGCGCGGCGCCGAAAATGTCGTCCACGGCTTTGTCGATGCCGGATCGAACGGCAGCC
TGATCGTCGGCTGTTACCTCTATGATACGGCGCTGGGCAGCGAACTGGTGCGCAAGGGGTTCGAGATACAGCCCGCCGAC
TGGCGCCGCGCCGCGCATAAATGCGCCGACGCCATCTATTCGCGCCTGTCGGGCGAGGCGCCCTTCTTCGACAGCCGCGT
CGCCTATATCGCCGAGAGCGGGCCCAAGGGGAACCGCATCAAACGGCTCGCGATCATGGATTCGGACGGCGGCAATCACC
GCTTCATCACCAACGGTCAGGCGCTGGCGCTCAGTCCGCGTTTTTCACCGGATTACAAGAAGATCGTTTATGTCAGCTAT
TTGAATGACCGGGTTCGCGTCTTCATCTATGACGTTGCGTCGGGGACACAGCGGCTGGTGACTGAAAGCCGCAATCCGAC
CTTTGCCCCGCGCTGGTCGCCCGACGGGACGCAGATCCTCTATTCGATGGCGGTCGGCGGCAACACCGACATCTATCGCA
TTTCGGCGAACGGCGGCACGCCGGTGCGACTGACCACCGCGCCGGGGATCGACGTCGGCGGCAGCTTTTCGCCCGACGGG
CGCAAGATCGTGTTCGAAAGCGACCGGTCGGGCAGCCAGCAGCTTTATGTGATGAACATTGACGGGTCGAACCAGCAGCG
GATCAGCTTTGGCGGCGGACGCTATGCCACCCCTGAATGGTCGCCGCGCGGCGACCTGATCGCCTTCACCCGCATGGGCG
GCGGCGAGTTTCGCATCGGCGTGATGACGCCGTCGGGCGGTGGGGTGCGGATGCTGACGAACGGCTGGCAGGACGAGGCG
CCGACCTGGTCGCCGAACGGCCGCGTCATCCAGTTCTTCCGCACCACGCCGGGCCGCGAGGGCAGGTCCAGCCTGTGGCA
GGTTGACCTGACCGGCGTGAACCTGCGCCGCCTGCCGACCCCGCAGGACGGATCGGACCCCAGCTGGGGGCCGGTTCTGC
CTTGA

Upstream 100 bases:

>100_bases
CCAAACGCGCGGTCGAACTTGCCGCGCCTTTCGACCTGCCCGAAGAAAATTACAGCTTCTGGCAGAATTACACGCTCGAT
TTCATCAAGAGGTAAGCAAC

Downstream 100 bases:

>100_bases
CAAGCAAGGAACCAAAAGGGCTCCATCGGGTTCCCCTATCGCGTCGCTACGCCGCGAAACCGCCCGTTGGAGAGAGTTTC
TCCACAATAACAAGAGGACA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSLRPISLMLALLLTSAPALAQSAPTPPATVQTEPRETIEGTLSQERTVIGIPSFATASVQTVAGLRTDALGRQLADVIA
ADLERSGLFEPIGPNGVRAIGRAEVQAPRFGEWQARGAENVVHGFVDAGSNGSLIVGCYLYDTALGSELVRKGFEIQPAD
WRRAAHKCADAIYSRLSGEAPFFDSRVAYIAESGPKGNRIKRLAIMDSDGGNHRFITNGQALALSPRFSPDYKKIVYVSY
LNDRVRVFIYDVASGTQRLVTESRNPTFAPRWSPDGTQILYSMAVGGNTDIYRISANGGTPVRLTTAPGIDVGGSFSPDG
RKIVFESDRSGSQQLYVMNIDGSNQQRISFGGGRYATPEWSPRGDLIAFTRMGGGEFRIGVMTPSGGGVRMLTNGWQDEA
PTWSPNGRVIQFFRTTPGREGRSSLWQVDLTGVNLRRLPTPQDGSDPSWGPVLP

Sequences:

>Translated_454_residues
MSLRPISLMLALLLTSAPALAQSAPTPPATVQTEPRETIEGTLSQERTVIGIPSFATASVQTVAGLRTDALGRQLADVIA
ADLERSGLFEPIGPNGVRAIGRAEVQAPRFGEWQARGAENVVHGFVDAGSNGSLIVGCYLYDTALGSELVRKGFEIQPAD
WRRAAHKCADAIYSRLSGEAPFFDSRVAYIAESGPKGNRIKRLAIMDSDGGNHRFITNGQALALSPRFSPDYKKIVYVSY
LNDRVRVFIYDVASGTQRLVTESRNPTFAPRWSPDGTQILYSMAVGGNTDIYRISANGGTPVRLTTAPGIDVGGSFSPDG
RKIVFESDRSGSQQLYVMNIDGSNQQRISFGGGRYATPEWSPRGDLIAFTRMGGGEFRIGVMTPSGGGVRMLTNGWQDEA
PTWSPNGRVIQFFRTTPGREGRSSLWQVDLTGVNLRRLPTPQDGSDPSWGPVLP
>Mature_453_residues
SLRPISLMLALLLTSAPALAQSAPTPPATVQTEPRETIEGTLSQERTVIGIPSFATASVQTVAGLRTDALGRQLADVIAA
DLERSGLFEPIGPNGVRAIGRAEVQAPRFGEWQARGAENVVHGFVDAGSNGSLIVGCYLYDTALGSELVRKGFEIQPADW
RRAAHKCADAIYSRLSGEAPFFDSRVAYIAESGPKGNRIKRLAIMDSDGGNHRFITNGQALALSPRFSPDYKKIVYVSYL
NDRVRVFIYDVASGTQRLVTESRNPTFAPRWSPDGTQILYSMAVGGNTDIYRISANGGTPVRLTTAPGIDVGGSFSPDGR
KIVFESDRSGSQQLYVMNIDGSNQQRISFGGGRYATPEWSPRGDLIAFTRMGGGEFRIGVMTPSGGGVRMLTNGWQDEAP
TWSPNGRVIQFFRTTPGREGRSSLWQVDLTGVNLRRLPTPQDGSDPSWGPVLP

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=381, Percent_Identity=28.8713910761155, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32564215, Length=187, Percent_Identity=28.3422459893048, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32564217, Length=187, Percent_Identity=28.3422459893048, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_SPHAL (Q1GWC1)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615384.1
- ProteinModelPortal:   Q1GWC1
- SMR:   Q1GWC1
- GeneID:   4082359
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0328
- NMPDR:   fig|317655.9.peg.303
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK05137
- BioCyc:   SALA317655:SALA_0328-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 49027; Mature: 48896

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRPISLMLALLLTSAPALAQSAPTPPATVQTEPRETIEGTLSQERTVIGIPSFATASV
CCCCHHHHHHHHHHHCCCHHHHCCCCCCCEECCCCHHHHHHHHCCCCEEEECCCCCHHHH
QTVAGLRTDALGRQLADVIAADLERSGLFEPIGPNGVRAIGRAEVQAPRFGEWQARGAEN
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHCCCCCCCCCHHCCCCHH
VVHGFVDAGSNGSLIVGCYLYDTALGSELVRKGFEIQPADWRRAAHKCADAIYSRLSGEA
HHHHEEECCCCCCEEEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PFFDSRVAYIAESGPKGNRIKRLAIMDSDGGNHRFITNGQALALSPRFSPDYKKIVYVSY
CCCCCEEEEEECCCCCCCCEEEEEEEECCCCCEEEEECCCEEEECCCCCCCCEEEEEEEE
LNDRVRVFIYDVASGTQRLVTESRNPTFAPRWSPDGTQILYSMAVGGNTDIYRISANGGT
ECCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCC
PVRLTTAPGIDVGGSFSPDGRKIVFESDRSGSQQLYVMNIDGSNQQRISFGGGRYATPEW
EEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCEECCCCC
SPRGDLIAFTRMGGGEFRIGVMTPSGGGVRMLTNGWQDEAPTWSPNGRVIQFFRTTPGRE
CCCCCEEEEEECCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCC
GRSSLWQVDLTGVNLRRLPTPQDGSDPSWGPVLP
CCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLRPISLMLALLLTSAPALAQSAPTPPATVQTEPRETIEGTLSQERTVIGIPSFATASV
CCCHHHHHHHHHHHCCCHHHHCCCCCCCEECCCCHHHHHHHHCCCCEEEECCCCCHHHH
QTVAGLRTDALGRQLADVIAADLERSGLFEPIGPNGVRAIGRAEVQAPRFGEWQARGAEN
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHCCCCCCCCCHHCCCCHH
VVHGFVDAGSNGSLIVGCYLYDTALGSELVRKGFEIQPADWRRAAHKCADAIYSRLSGEA
HHHHEEECCCCCCEEEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PFFDSRVAYIAESGPKGNRIKRLAIMDSDGGNHRFITNGQALALSPRFSPDYKKIVYVSY
CCCCCEEEEEECCCCCCCCEEEEEEEECCCCCEEEEECCCEEEECCCCCCCCEEEEEEEE
LNDRVRVFIYDVASGTQRLVTESRNPTFAPRWSPDGTQILYSMAVGGNTDIYRISANGGT
ECCCEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCC
PVRLTTAPGIDVGGSFSPDGRKIVFESDRSGSQQLYVMNIDGSNQQRISFGGGRYATPEW
EEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCEECCCCC
SPRGDLIAFTRMGGGEFRIGVMTPSGGGVRMLTNGWQDEAPTWSPNGRVIQFFRTTPGRE
CCCCCEEEEEECCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCC
GRSSLWQVDLTGVNLRRLPTPQDGSDPSWGPVLP
CCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA