BacMap

Definition	Sphingopyxis alaskensis RB2256, complete genome.
Accession	NC_008048
Length	3,345,170

Click here to switch to the map view.

The map label for this gene is hetN [H]

Identifier: 103485741

GI number: 103485741

Start: 249945

End: 250763

Strand: Reverse

Name: hetN [H]

Synonym: Sala_0245

Alternate gene names: 103485741

Gene position: 250763-249945 (Counterclockwise)

Preceding gene: 103485743

Following gene: 103485737

Centisome position: 7.5

GC content: 68.25

Gene sequence:

>819_bases
ATGAAAGATCAAATCTGGTGGGTCACGGGCGCTTCCTCGGGGATCGGCGCGGCGCTGGCACGGGCGCTGACAGCGCGCGG
GGCAAAGCTGATCCTGTCGGGCCGCAATATCGCCGCGCTGGAGGCGGTGGCGAAGGATTGCGGCGCGGGCACGATGATCC
TGCCGTTCGAGGCGACCGACTATGCCGCGCTGCCCGCGATCGTCCGGCAGGCGTGGGACTGGCAGGGGCGGATCGACGGG
CTGGTCAACAATGCCGGCATTTCGCAGCGCAGCCTGGCGATTGAGACCGACTTTTCGGTCTATCAGCGCATCATCGGCGT
CGACCTGCTCGCCCCGATCGCGCTTACCCAGCAGCTTTTGCCGCGCATGATCGAAGCGGGCGGCGGGCAGATCATCGCCA
TATCGAGCGTCGCGGGCATCGCCGGCGTGCCGCTGCGCAGCGCCTATTCGGCGGCGAAGCACGGCCTCATCGGCTATCAC
GACGCGGTGCGCGCCGAAAATGAGCATCTGGGGCTGAAGGTGCTGGTCGTCGCGCCGGGGTCGGTGCGCACCAATGTCAG
CCGCAACGCGCTGAACGCCGACGGCAGCGTGCGGGGGGAAAGCGACACGGCGATCGACAACGGCCTGTCGCCGGACGATG
CGGCGGCGCAGATGCTCGCCGCGGTCGATGCAGGCAAGCGCGAGATCGTCGTCGCCGAAGGCATGGAGGCCGCGATCGCC
GACCTGCGCCGCAGCGATCCCGACGCATTGTTCGACCGGATGAGTGTGATGGTGCAGGCGGGCTATGCGCAGCAGATGAA
GGCGGGCGGCACGGCCTGA

Upstream 100 bases:

>100_bases
AACAAAATTGCCACCCCCGCACGGAAATGTGCGGGTCGGCGCCTTGACGGCTCCTCGCACCCGTTTCACCTTGCAACGAA
AGTGAAGATAGGGAGCGGAT

Downstream 100 bases:

>100_bases
GCGCCAGCCCCACGAAAAAGGGCGGCCCGTTGCCGGACCGCCCTCCTCTTCTCCACCCGTCGGTGGAAAAAGCTCTTAAC
GCTTCGAGAACTGGAAGCTG

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKDQIWWVTGASSGIGAALARALTARGAKLILSGRNIAALEAVAKDCGAGTMILPFEATDYAALPAIVRQAWDWQGRIDG
LVNNAGISQRSLAIETDFSVYQRIIGVDLLAPIALTQQLLPRMIEAGGGQIIAISSVAGIAGVPLRSAYSAAKHGLIGYH
DAVRAENEHLGLKVLVVAPGSVRTNVSRNALNADGSVRGESDTAIDNGLSPDDAAAQMLAAVDAGKREIVVAEGMEAAIA
DLRRSDPDALFDRMSVMVQAGYAQQMKAGGTA

Sequences:

>Translated_272_residues
MKDQIWWVTGASSGIGAALARALTARGAKLILSGRNIAALEAVAKDCGAGTMILPFEATDYAALPAIVRQAWDWQGRIDG
LVNNAGISQRSLAIETDFSVYQRIIGVDLLAPIALTQQLLPRMIEAGGGQIIAISSVAGIAGVPLRSAYSAAKHGLIGYH
DAVRAENEHLGLKVLVVAPGSVRTNVSRNALNADGSVRGESDTAIDNGLSPDDAAAQMLAAVDAGKREIVVAEGMEAAIA
DLRRSDPDALFDRMSVMVQAGYAQQMKAGGTA
>Mature_272_residues
MKDQIWWVTGASSGIGAALARALTARGAKLILSGRNIAALEAVAKDCGAGTMILPFEATDYAALPAIVRQAWDWQGRIDG
LVNNAGISQRSLAIETDFSVYQRIIGVDLLAPIALTQQLLPRMIEAGGGQIIAISSVAGIAGVPLRSAYSAAKHGLIGYH
DAVRAENEHLGLKVLVVAPGSVRTNVSRNALNADGSVRGESDTAIDNGLSPDDAAAQMLAAVDAGKREIVVAEGMEAAIA
DLRRSDPDALFDRMSVMVQAGYAQQMKAGGTA

Specific function: May be involved in repressing heterocyst differentiation and may be essential for preventing all vegetative cells from differentiating [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI20149619, Length=264, Percent_Identity=37.5, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI7706318, Length=199, Percent_Identity=39.1959798994975, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI157739938, Length=260, Percent_Identity=27.6923076923077, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI40254992, Length=187, Percent_Identity=29.4117647058824, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI142976729, Length=275, Percent_Identity=25.0909090909091, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI31542939, Length=191, Percent_Identity=31.413612565445, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI150247226, Length=204, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI32483357, Length=195, Percent_Identity=29.7435897435897, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI44680136, Length=190, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI44680133, Length=190, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI17738292, Length=190, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI32455239, Length=170, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI5031765, Length=170, Percent_Identity=32.3529411764706, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI210032110, Length=217, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI224922801, Length=164, Percent_Identity=32.3170731707317, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI109715829, Length=229, Percent_Identity=27.0742358078603, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI19743808, Length=199, Percent_Identity=29.1457286432161, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4503817, Length=180, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI22507405, Length=190, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI40548397, Length=221, Percent_Identity=26.2443438914027, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI214010158, Length=221, Percent_Identity=26.2443438914027, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI214010156, Length=221, Percent_Identity=26.2443438914027, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI22758144, Length=221, Percent_Identity=26.2443438914027, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI7705855, Length=187, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI5031737, Length=195, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI50726952, Length=196, Percent_Identity=30.6122448979592, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787335, Length=188, Percent_Identity=35.1063829787234, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI87082100, Length=187, Percent_Identity=32.620320855615, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786701, Length=203, Percent_Identity=30.0492610837438, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87082160, Length=183, Percent_Identity=30.6010928961749, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790717, Length=185, Percent_Identity=28.1081081081081, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788459, Length=212, Percent_Identity=30.188679245283, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17570069, Length=226, Percent_Identity=33.1858407079646, Blast_Score=126, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71997402, Length=251, Percent_Identity=31.0756972111554, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17508895, Length=185, Percent_Identity=31.8918918918919, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71994604, Length=192, Percent_Identity=32.2916666666667, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=191, Percent_Identity=30.8900523560209, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=198, Percent_Identity=30.8080808080808, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=193, Percent_Identity=28.4974093264249, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=190, Percent_Identity=29.4736842105263, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=192, Percent_Identity=31.7708333333333, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=192, Percent_Identity=31.7708333333333, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=261, Percent_Identity=27.9693486590038, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=187, Percent_Identity=31.0160427807487, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=185, Percent_Identity=28.1081081081081, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=186, Percent_Identity=32.7956989247312, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=258, Percent_Identity=26.7441860465116, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=253, Percent_Identity=29.2490118577075, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=195, Percent_Identity=29.2307692307692, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17507613, Length=199, Percent_Identity=26.1306532663317, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI32563809, Length=199, Percent_Identity=26.1306532663317, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=201, Percent_Identity=28.3582089552239, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323882, Length=258, Percent_Identity=29.0697674418605, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322227, Length=195, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21358495, Length=252, Percent_Identity=35.7142857142857, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24580925, Length=203, Percent_Identity=33.4975369458128, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI23397609, Length=190, Percent_Identity=38.4210526315789, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24644339, Length=196, Percent_Identity=33.6734693877551, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571526, Length=192, Percent_Identity=31.7708333333333, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357041, Length=192, Percent_Identity=31.7708333333333, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24640442, Length=190, Percent_Identity=31.5789473684211, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24649181, Length=185, Percent_Identity=27.027027027027, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355319, Length=189, Percent_Identity=26.4550264550265, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24641232, Length=204, Percent_Identity=27.4509803921569, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221510726, Length=175, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 28273; Mature: 28273

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDQIWWVTGASSGIGAALARALTARGAKLILSGRNIAALEAVAKDCGAGTMILPFEATD
CCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCCC
YAALPAIVRQAWDWQGRIDGLVNNAGISQRSLAIETDFSVYQRIIGVDLLAPIALTQQLL
HHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
PRMIEAGGGQIIAISSVAGIAGVPLRSAYSAAKHGLIGYHDAVRAENEHLGLKVLVVAPG
HHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCC
SVRTNVSRNALNADGSVRGESDTAIDNGLSPDDAAAQMLAAVDAGKREIVVAEGMEAAIA
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHH
DLRRSDPDALFDRMSVMVQAGYAQQMKAGGTA
HHHCCCHHHHHHHHHHHHHHCHHHHHHCCCCC
>Mature Secondary Structure
MKDQIWWVTGASSGIGAALARALTARGAKLILSGRNIAALEAVAKDCGAGTMILPFEATD
CCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCCC
YAALPAIVRQAWDWQGRIDGLVNNAGISQRSLAIETDFSVYQRIIGVDLLAPIALTQQLL
HHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
PRMIEAGGGQIIAISSVAGIAGVPLRSAYSAAKHGLIGYHDAVRAENEHLGLKVLVVAPG
HHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCC
SVRTNVSRNALNADGSVRGESDTAIDNGLSPDDAAAQMLAAVDAGKREIVVAEGMEAAIA
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHH
DLRRSDPDALFDRMSVMVQAGYAQQMKAGGTA
HHHCCCHHHHHHHHHHHHHHCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8157596; 11759840 [H]