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The map label for this gene is bla [H]

Identifier: 103485670

GI number: 103485670

Start: 178424

End: 179590

Strand: Direct

Name: bla [H]

Synonym: Sala_0174

Alternate gene names: 103485670

Gene position: 178424-179590 (Clockwise)

Preceding gene: 103485669

Following gene: 103485671

Centisome position: 5.33

GC content: 67.95

Gene sequence:

>1167_bases
ATGAAGATTGAGTTTTCCGGCAGGCCGCTCCTCGCGATGACGATGAGCGCCGCGGTGCTCGCGGGCTGCGTCAGCAGCGC
CGCCGTCATCCCGCAACCGCGCGCCCAGGCGCCGGTCGAGCAGACGTCGCAACCCCGGGCAGTTCGCGGGCCATCCGGGT
CGGTGACAGTGCCGATCGGCCAGCCGGTTCCGTCGGCCATGCCGCGTCCTGCCGGACCGCTTGACCCCGGCTTTCGCCGC
GCCCCCGCCGGATTGCAGGATCGTATCCATGCGTTGTGGAGCGCCTTTCCCGGCAAAACCGGAATCGCCGTGCAGCGGAT
CGATGGCGAATGGACCTTGTCGCATCGCGGCGGCGACCTGTTTCCGCAGCAGAGTGTCTCGAAGCTGTGGGTCGCGCTCA
CCGTGCTCGACGCCGTCGATCAGGGGCGGTTGACGCTCGATCAGCGCGTGCGCATCGGGCCTGAGGATCTGACGCTTTTC
TATCAACCGATCGCGGCGCGCGTGCGCGCGGAAGGATCGGTGACGCTGAGCGTCCGCGACCTCATCGAGATTGCCATTAC
GCAAAGTGACAACACAGCGAACGACAGTCTGCTGCGCACGGTCGGCGGCCCCGACGCGGTGCGGCGTTTCATCGCCAAGA
AGGATCTGGGCGCGATCCGCTTCGGCCCCGGCGAGCGGCTGCTCCAGGCAGGGACCGCGGGGCTGAAGTGGCAGCAGGCC
TATTCGGTTGGGCGCGCCTTTCAACAGGCACGCGCCGCGCTTCCCGATGCGACGCGGCGCGCGGCGCGCAACGCCTATCT
CGCCAATCCCCCCGATGGCGCCAGCCCCACCGCGATCGCCAATGCGCTGACGCGTCTCGCGCGCGGCGCGTTGCTGTCGC
CCGAATCGACCGAATATCTGCTGGGGGTGATGAGCCGGACCAAAAGCGGCCCCCGGCGCCTGAAGGCGGGGTTGCCCGCC
GATTGGAAGTTCCTGCACAAGACGGGTACGGGGCAGGACTATAAGGGTTCGACAGCGGGCTATAACGACATCGGCATCGC
GACCGCACCCGATGGAACGCGCTATGCCATCGTCGTCCTGCTCGGCGACACGAGCGCCCCGGTGCCCGCGCGCATGGAAC
TGATGCAGGCGGTGTCGAGCGCCGTCGCGGCCTATCACGGAAAATGA

Upstream 100 bases:

>100_bases
TCGTTCGTCGCAAATCGCTGGTCCGGCGTAGACGGCTGCGCGACCAACGGAGCAACCGAAGGGGTCGGGGTCATATACAG
GTCGCGTGCGCGGGGTTCGA

Downstream 100 bases:

>100_bases
CATCCCCATGAATCGCGCCTTGCCGCCGGCCGCCGCGCTGGTACTCCTTCTGTCGGCCTGCGCGACGCCCGAGGCGCGGC
TGCGCACCGGGCTCAACAAC

Product: Beta-lactamase

Products: NA

Alternate protein names: IRT-4; Penicillinase; TEM-1; TEM-16/CAZ-7; TEM-2; TEM-24/CAZ-6; TEM-3; TEM-4; TEM-5; TEM-6; TEM-8/CAZ-2 [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKIEFSGRPLLAMTMSAAVLAGCVSSAAVIPQPRAQAPVEQTSQPRAVRGPSGSVTVPIGQPVPSAMPRPAGPLDPGFRR
APAGLQDRIHALWSAFPGKTGIAVQRIDGEWTLSHRGGDLFPQQSVSKLWVALTVLDAVDQGRLTLDQRVRIGPEDLTLF
YQPIAARVRAEGSVTLSVRDLIEIAITQSDNTANDSLLRTVGGPDAVRRFIAKKDLGAIRFGPGERLLQAGTAGLKWQQA
YSVGRAFQQARAALPDATRRAARNAYLANPPDGASPTAIANALTRLARGALLSPESTEYLLGVMSRTKSGPRRLKAGLPA
DWKFLHKTGTGQDYKGSTAGYNDIGIATAPDGTRYAIVVLLGDTSAPVPARMELMQAVSSAVAAYHGK

Sequences:

>Translated_388_residues
MKIEFSGRPLLAMTMSAAVLAGCVSSAAVIPQPRAQAPVEQTSQPRAVRGPSGSVTVPIGQPVPSAMPRPAGPLDPGFRR
APAGLQDRIHALWSAFPGKTGIAVQRIDGEWTLSHRGGDLFPQQSVSKLWVALTVLDAVDQGRLTLDQRVRIGPEDLTLF
YQPIAARVRAEGSVTLSVRDLIEIAITQSDNTANDSLLRTVGGPDAVRRFIAKKDLGAIRFGPGERLLQAGTAGLKWQQA
YSVGRAFQQARAALPDATRRAARNAYLANPPDGASPTAIANALTRLARGALLSPESTEYLLGVMSRTKSGPRRLKAGLPA
DWKFLHKTGTGQDYKGSTAGYNDIGIATAPDGTRYAIVVLLGDTSAPVPARMELMQAVSSAVAAYHGK
>Mature_388_residues
MKIEFSGRPLLAMTMSAAVLAGCVSSAAVIPQPRAQAPVEQTSQPRAVRGPSGSVTVPIGQPVPSAMPRPAGPLDPGFRR
APAGLQDRIHALWSAFPGKTGIAVQRIDGEWTLSHRGGDLFPQQSVSKLWVALTVLDAVDQGRLTLDQRVRIGPEDLTLF
YQPIAARVRAEGSVTLSVRDLIEIAITQSDNTANDSLLRTVGGPDAVRRFIAKKDLGAIRFGPGERLLQAGTAGLKWQQA
YSVGRAFQQARAALPDATRRAARNAYLANPPDGASPTAIANALTRLARGALLSPESTEYLLGVMSRTKSGPRRLKAGLPA
DWKFLHKTGTGQDYKGSTAGYNDIGIATAPDGTRYAIVVLLGDTSAPVPARMELMQAVSSAVAAYHGK

Specific function: TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotax

COG id: COG2367

COG function: function code V; Beta-lactamase class A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 41065; Mature: 41065

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEFSGRPLLAMTMSAAVLAGCVSSAAVIPQPRAQAPVEQTSQPRAVRGPSGSVTVPIG
CEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEECCCCCEEEECC
QPVPSAMPRPAGPLDPGFRRAPAGLQDRIHALWSAFPGKTGIAVQRIDGEWTLSHRGGDL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCC
FPQQSVSKLWVALTVLDAVDQGRLTLDQRVRIGPEDLTLFYQPIAARVRAEGSVTLSVRD
CCHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHH
LIEIAITQSDNTANDSLLRTVGGPDAVRRFIAKKDLGAIRFGPGERLLQAGTAGLKWQQA
EEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCHHHHH
YSVGRAFQQARAALPDATRRAARNAYLANPPDGASPTAIANALTRLARGALLSPESTEYL
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LGVMSRTKSGPRRLKAGLPADWKFLHKTGTGQDYKGSTAGYNDIGIATAPDGTRYAIVVL
HHHHHHCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEE
LGDTSAPVPARMELMQAVSSAVAAYHGK
ECCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIEFSGRPLLAMTMSAAVLAGCVSSAAVIPQPRAQAPVEQTSQPRAVRGPSGSVTVPIG
CEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEECCCCCEEEECC
QPVPSAMPRPAGPLDPGFRRAPAGLQDRIHALWSAFPGKTGIAVQRIDGEWTLSHRGGDL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCC
FPQQSVSKLWVALTVLDAVDQGRLTLDQRVRIGPEDLTLFYQPIAARVRAEGSVTLSVRD
CCHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHH
LIEIAITQSDNTANDSLLRTVGGPDAVRRFIAKKDLGAIRFGPGERLLQAGTAGLKWQQA
EEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCEEECCHHHHHHHCCCCCHHHHH
YSVGRAFQQARAALPDATRRAARNAYLANPPDGASPTAIANALTRLARGALLSPESTEYL
HHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LGVMSRTKSGPRRLKAGLPADWKFLHKTGTGQDYKGSTAGYNDIGIATAPDGTRYAIVVL
HHHHHHCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEE
LGDTSAPVPARMELMQAVSSAVAAYHGK
ECCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 358200; 383387; 3019092; 358199; 1331747; 2550326; 1665171; 1416873; 8056282; 1544485; 8356032; 8605632; 9485412; 10423234 [H]