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The map label for this gene is glnA [H]

Identifier: 103485645

GI number: 103485645

Start: 144103

End: 145515

Strand: Direct

Name: glnA [H]

Synonym: Sala_0149

Alternate gene names: 103485645

Gene position: 144103-145515 (Clockwise)

Preceding gene: 103485644

Following gene: 103485646

Centisome position: 4.31

GC content: 61.78

Gene sequence:

>1413_bases
ATGGCTACGAAGCCCAAGGATATCATCGCCCGGATCAAGGACAACGACATCGAGTGGGTCGACCTGCGCTTCACCGACCC
CAAGGGCAAGTGGCAGCACCTGACCATGTGCGCCGGGGTGATCGACGAGGATGCGCTCGAAGACGGGCTGATGTTCGACG
GTTCGTCGATCGAGGGCTGGAAGGCGATCAACGAAAGCGACATGATCCTGAAGCCCGATCTCGACGCCGTCTATGACGAT
CCCTTTTCGGCGACGCCGATGCTGGTGATCTTCTGCGACATCGTCGAGCCGTCGACCGGCGAGGGCTATGCCCGCGATCC
GCGCACGACGGCGAAGCGCGCCGAGGCCTATCTCGGATCGACCGGCATCGGCGACACCGTCTATGTCGGCCCCGAAGCCG
AATTCTTCATGTTCGACGATGTCCGCTTCGAAACCGGCTATAACAAGTCGGGCTTCGAGATCGACGACATCGAGCTGCCG
ACCAACACCGGCCGCAGCTATGAGGGCGGCAACCTCGCCCACCGCCCGCGCGCCAAGGGCGGCTATTTCCCCGTCGCGCC
GGTCGACAGCGCGGTCGACATCCGCGCCGAAATGGTCTCGACGATGCTCGAAATGGGCCTGCCGTGCGACAAGCACCACC
ATGAGGTCGCCGCGGCGCAGCACGAGCTGGGCCTGACCTTCGGCACGCTGACGCAGACCGCCGACCGTATGCAGATCTAT
AAATATGTCGTCCACCAGGTCGCGCACGCCTATGGCAAGACCGCAACCTTCATGCCCAAACCGATCAAGGACGATAACGG
TTCGGGGATGCACACGCACATCTCGATCTGGGAAAAGGGCAAGCCGCTCTTTGCGGGCAATGGCTATGCCGGTCTCAGCG
ACATGTGCCTCTATTTCATCGGCGGCGTCATCAAGCACGCCAAGGCGCTCAACGCCTTCACCAACCCGACGACGAACAGC
TACAAGCGCCTTGTTCCGGGCTTCGAAGCGCCGGTGCTGCTCGCTTATTCGAGCCGCAACCGCTCGGCCTCGTGCCGCAT
TCCCTATGGCGCGGGCGCCAAGGCGAAGCGCGTCGAGTTCCGCTTCCCCGACGCGATGGCGAACCCCTATCTCTGCTATT
CGGCGCTGCTGATGGCGGGGCTCGACGGCATTCAGAACAAGATCCATCCCGGCGACGCGATGGACAAGAATCTCTATGAC
CTGCCGCCCGAAGAGCTTTCCGAAGTGCCGACCGTGTGCGGCAGCTTGCGTGAAGCGCTCGACAGCCTGATGGCCGATCA
CGACTTCCTTTTGAAGGGCGACGTGTTTTCGAAGGACCAGATCGAGGCCTATGTCGAGCTCAAATGGAACGAAGTCTATC
GGTTCGAACAGACGCCGAGCCCGGTCGAGTTCGACATGTATTACAGCGCCTGA

Upstream 100 bases:

>100_bases
CCGGCGAACGCAACGAGGACGCGCTCTGACCGCCCCGCACCCATTTCATCCCAGCAATCCCCGGCACGCCGGACCATCAC
GCAAGAAGGAAGCATAGACC

Downstream 100 bases:

>100_bases
TCGACCAGCGGGTCAGTAGCGCCGACAGGGGCGTCCGGTTCGGGGGAGCCGGACGCCCCTTTTCGTGGCGCGTTGACGTG
CCAGGTAGTTGCGCGCGAGC

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MATKPKDIIARIKDNDIEWVDLRFTDPKGKWQHLTMCAGVIDEDALEDGLMFDGSSIEGWKAINESDMILKPDLDAVYDD
PFSATPMLVIFCDIVEPSTGEGYARDPRTTAKRAEAYLGSTGIGDTVYVGPEAEFFMFDDVRFETGYNKSGFEIDDIELP
TNTGRSYEGGNLAHRPRAKGGYFPVAPVDSAVDIRAEMVSTMLEMGLPCDKHHHEVAAAQHELGLTFGTLTQTADRMQIY
KYVVHQVAHAYGKTATFMPKPIKDDNGSGMHTHISIWEKGKPLFAGNGYAGLSDMCLYFIGGVIKHAKALNAFTNPTTNS
YKRLVPGFEAPVLLAYSSRNRSASCRIPYGAGAKAKRVEFRFPDAMANPYLCYSALLMAGLDGIQNKIHPGDAMDKNLYD
LPPEELSEVPTVCGSLREALDSLMADHDFLLKGDVFSKDQIEAYVELKWNEVYRFEQTPSPVEFDMYYSA

Sequences:

>Translated_470_residues
MATKPKDIIARIKDNDIEWVDLRFTDPKGKWQHLTMCAGVIDEDALEDGLMFDGSSIEGWKAINESDMILKPDLDAVYDD
PFSATPMLVIFCDIVEPSTGEGYARDPRTTAKRAEAYLGSTGIGDTVYVGPEAEFFMFDDVRFETGYNKSGFEIDDIELP
TNTGRSYEGGNLAHRPRAKGGYFPVAPVDSAVDIRAEMVSTMLEMGLPCDKHHHEVAAAQHELGLTFGTLTQTADRMQIY
KYVVHQVAHAYGKTATFMPKPIKDDNGSGMHTHISIWEKGKPLFAGNGYAGLSDMCLYFIGGVIKHAKALNAFTNPTTNS
YKRLVPGFEAPVLLAYSSRNRSASCRIPYGAGAKAKRVEFRFPDAMANPYLCYSALLMAGLDGIQNKIHPGDAMDKNLYD
LPPEELSEVPTVCGSLREALDSLMADHDFLLKGDVFSKDQIEAYVELKWNEVYRFEQTPSPVEFDMYYSA
>Mature_469_residues
ATKPKDIIARIKDNDIEWVDLRFTDPKGKWQHLTMCAGVIDEDALEDGLMFDGSSIEGWKAINESDMILKPDLDAVYDDP
FSATPMLVIFCDIVEPSTGEGYARDPRTTAKRAEAYLGSTGIGDTVYVGPEAEFFMFDDVRFETGYNKSGFEIDDIELPT
NTGRSYEGGNLAHRPRAKGGYFPVAPVDSAVDIRAEMVSTMLEMGLPCDKHHHEVAAAQHELGLTFGTLTQTADRMQIYK
YVVHQVAHAYGKTATFMPKPIKDDNGSGMHTHISIWEKGKPLFAGNGYAGLSDMCLYFIGGVIKHAKALNAFTNPTTNSY
KRLVPGFEAPVLLAYSSRNRSASCRIPYGAGAKAKRVEFRFPDAMANPYLCYSALLMAGLDGIQNKIHPGDAMDKNLYDL
PPEELSEVPTVCGSLREALDSLMADHDFLLKGDVFSKDQIEAYVELKWNEVYRFEQTPSPVEFDMYYSA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=463, Percent_Identity=61.7710583153348, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI87081870, Length=310, Percent_Identity=27.4193548387097, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281360453, Length=342, Percent_Identity=25.7309941520468, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136208, Length=342, Percent_Identity=25.7309941520468, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580558, Length=342, Percent_Identity=25.7309941520468, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52143; Mature: 52012

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKPKDIIARIKDNDIEWVDLRFTDPKGKWQHLTMCAGVIDEDALEDGLMFDGSSIEGW
CCCCCHHHHHEECCCCCEEEEEEEECCCCCCEEHHHHHHHHCHHHHHCCCEECCCCCCCC
KAINESDMILKPDLDAVYDDPFSATPMLVIFCDIVEPSTGEGYARDPRTTAKRAEAYLGS
CCCCCCCCEECCCCHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
TGIGDTVYVGPEAEFFMFDDVRFETGYNKSGFEIDDIELPTNTGRSYEGGNLAHRPRAKG
CCCCCEEEECCCCCEEEEECEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
GYFPVAPVDSAVDIRAEMVSTMLEMGLPCDKHHHEVAAAQHELGLTFGTLTQTADRMQIY
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KYVVHQVAHAYGKTATFMPKPIKDDNGSGMHTHISIWEKGKPLFAGNGYAGLSDMCLYFI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHH
GGVIKHAKALNAFTNPTTNSYKRLVPGFEAPVLLAYSSRNRSASCRIPYGAGAKAKRVEF
HHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEEE
RFPDAMANPYLCYSALLMAGLDGIQNKIHPGDAMDKNLYDLPPEELSEVPTVCGSLREAL
ECCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DSLMADHDFLLKGDVFSKDQIEAYVELKWNEVYRFEQTPSPVEFDMYYSA
HHHHCCCCEEEECCCCCCCCEEEEEEEEECCEEEECCCCCCEEEEEEECC
>Mature Secondary Structure 
ATKPKDIIARIKDNDIEWVDLRFTDPKGKWQHLTMCAGVIDEDALEDGLMFDGSSIEGW
CCCCHHHHHEECCCCCEEEEEEEECCCCCCEEHHHHHHHHCHHHHHCCCEECCCCCCCC
KAINESDMILKPDLDAVYDDPFSATPMLVIFCDIVEPSTGEGYARDPRTTAKRAEAYLGS
CCCCCCCCEECCCCHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
TGIGDTVYVGPEAEFFMFDDVRFETGYNKSGFEIDDIELPTNTGRSYEGGNLAHRPRAKG
CCCCCEEEECCCCCEEEEECEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
GYFPVAPVDSAVDIRAEMVSTMLEMGLPCDKHHHEVAAAQHELGLTFGTLTQTADRMQIY
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KYVVHQVAHAYGKTATFMPKPIKDDNGSGMHTHISIWEKGKPLFAGNGYAGLSDMCLYFI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHH
GGVIKHAKALNAFTNPTTNSYKRLVPGFEAPVLLAYSSRNRSASCRIPYGAGAKAKRVEF
HHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCEEEEE
RFPDAMANPYLCYSALLMAGLDGIQNKIHPGDAMDKNLYDLPPEELSEVPTVCGSLREAL
ECCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DSLMADHDFLLKGDVFSKDQIEAYVELKWNEVYRFEQTPSPVEFDMYYSA
HHHHCCCCEEEECCCCCCCCEEEEEEEEECCEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]