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The map label for this gene is potB [H]

Identifier: 99082829

GI number: 99082829

Start: 3154473

End: 3156098

Strand: Direct

Name: potB [H]

Synonym: TM1040_2989

Alternate gene names: 99082829

Gene position: 3154473-3156098 (Clockwise)

Preceding gene: 99082828

Following gene: 99082830

Centisome position: 98.55

GC content: 58.73

Gene sequence:

>1626_bases
ATGAGCGATACAACCGATACCGGACCGGTCCTTGCTGCAGATGGCACGCCGCTAAAGCGCAGCCTTGCACGGGCGCTCCG
GATGCAGAAGATGCGCGCGCTGGCATTGATCGCCCCGCTTCTGCTCTTCGTTCTACTGACATTCATCCTGCCGATCGGCG
ACATGTTGTTTCGCTCGGTCGAGAACAGCATCGTTTCGGAGACGTTGCCGCGAACGACCGCAGCGGCGCAGTCTTATGAT
GCCGACAGCGGAGAAGCGCCGCCTGAATATGCATTTGACGCGCTGGCCTATGATCTGGTTGTTGCTGCCGAGAAGAAGAT
CCACACACGCGTGGGCTCGCGCCTGAACTACGAGAACGCTGGTATTTCTTCGCTGTTCCGCAAGGCTGGGCGCCGAGTCG
ATGACATTGGCGAGGTCTATCTCGATCAGTTTGTCGACCTTGACCCCGCTTGGGAAGACCCTCAGGCACTGGTCGCGCTT
TTTGCCGACCCCAGCTGGGCCGCCTCTGCCGGTGGCGAAAACGAAGCGCGGTTTGAGTATTCCTCTGTGGCTGGCGAGGC
GCTGCCGGGAGCGACGGATGCCTATGCTGCATGGGCGCGCACGATCCAAGAAGAAGATCAGGACAGCCCCACCGAAGAAG
AGCCATGGGCCCCGGTCTATATGGCGCTCTACAAGGATCTCGCGAAAAATCCGGATGCGGCATCTGCATTTGAGGGTGAA
ACTGCCGCGCGGCTTGCAGCAGCAGCAGCAGCCATCCAGTCCCCGGACTGGGAGACCATGTCCACGCGTGATGCTTTCAT
CAAAATCGATGACGACTGGGCCGACCCCGAAGTTTGGCGCACGATCAAGACCTACTCCAGTAAATACACCAATGGGTACT
TCTTGAACGCGGCCGACTTCCAGAAAGGTGAGGATGGCCCTGAGCTGCGCCCGGAACTTGAGCGCATCTATGGCACACTC
TTTCTGCGCACCATGATCATGTCGCTGGCTATCACCGTAAGCTGTATCGTGCTTGGCTACCCGGTGGCATGGATTCTGGC
GAACCTGCCGTCGCGCACCGCCAACGTCCTGATGATTCTCGTCCTGCTGCCGTTCTGGACCTCTCTGCTGGTGCGGACCT
CTGCATGGAAAGTCATGCTGCAGCAGCAGGGCGTCATCAATGATGTTCTGGTCTGGCTTGGACTGGTCGCTGACGACGCG
CGTCTTGTGATGATCAACAACCAGTTTGGTACAATTGTGGCGATGACGCACATCCTTCTGCCGTTCATGATCCTGCCGAT
GTATTCGGTGATGCAGACGATCAATCCGTCCTACCTGCGTGCGGCCAAGTCCCTTGGCGCGACCAACTGGACGGCCTTCT
GGCGGGTGTATTTCCCACAGTCGGTGCCGGGTATCGGCGCGGGTTCGATTCTCGTCTTCATCCTGGCCATTGGCTACTAC
ATCACGCCGGAACTCGTCGGCGGTACCAAGGGCGTATTCATCTCGAACCGGATCGCCTTCCACATCTCGCAATCCCTCAA
CTGGGGTCTTGCGGCGGCACTCGGGACAATCCTGCTGGTGGTGGTTCTGGTGCTCTACTGGGCCTACGACAAGATCGTCG
GCATCGACAACGTGAAGCTGGGATAA

Upstream 100 bases:

>100_bases
CTGATGTGAGCACTATCCGAGAGGGGGCCGAGCCGATTGGCCCTCTCTCCACCAAAGATAATAATAATACCCGCACCAAC
ACCCCGGGGGGCTCCTTTAC

Downstream 100 bases:

>100_bases
GAAAAACATGGCACTTACACCTGTTCAAAACCAAACCCCCGCTTTCACCGCCGCAGTGTCGGCCGGCGCAGGGGCCTTCT
TTGGCCTCTTCGTGGGTACA

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSDTTDTGPVLAADGTPLKRSLARALRMQKMRALALIAPLLLFVLLTFILPIGDMLFRSVENSIVSETLPRTTAAAQSYD
ADSGEAPPEYAFDALAYDLVVAAEKKIHTRVGSRLNYENAGISSLFRKAGRRVDDIGEVYLDQFVDLDPAWEDPQALVAL
FADPSWAASAGGENEARFEYSSVAGEALPGATDAYAAWARTIQEEDQDSPTEEEPWAPVYMALYKDLAKNPDAASAFEGE
TAARLAAAAAAIQSPDWETMSTRDAFIKIDDDWADPEVWRTIKTYSSKYTNGYFLNAADFQKGEDGPELRPELERIYGTL
FLRTMIMSLAITVSCIVLGYPVAWILANLPSRTANVLMILVLLPFWTSLLVRTSAWKVMLQQQGVINDVLVWLGLVADDA
RLVMINNQFGTIVAMTHILLPFMILPMYSVMQTINPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILAIGYY
ITPELVGGTKGVFISNRIAFHISQSLNWGLAAALGTILLVVVLVLYWAYDKIVGIDNVKLG

Sequences:

>Translated_541_residues
MSDTTDTGPVLAADGTPLKRSLARALRMQKMRALALIAPLLLFVLLTFILPIGDMLFRSVENSIVSETLPRTTAAAQSYD
ADSGEAPPEYAFDALAYDLVVAAEKKIHTRVGSRLNYENAGISSLFRKAGRRVDDIGEVYLDQFVDLDPAWEDPQALVAL
FADPSWAASAGGENEARFEYSSVAGEALPGATDAYAAWARTIQEEDQDSPTEEEPWAPVYMALYKDLAKNPDAASAFEGE
TAARLAAAAAAIQSPDWETMSTRDAFIKIDDDWADPEVWRTIKTYSSKYTNGYFLNAADFQKGEDGPELRPELERIYGTL
FLRTMIMSLAITVSCIVLGYPVAWILANLPSRTANVLMILVLLPFWTSLLVRTSAWKVMLQQQGVINDVLVWLGLVADDA
RLVMINNQFGTIVAMTHILLPFMILPMYSVMQTINPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILAIGYY
ITPELVGGTKGVFISNRIAFHISQSLNWGLAAALGTILLVVVLVLYWAYDKIVGIDNVKLG
>Mature_540_residues
SDTTDTGPVLAADGTPLKRSLARALRMQKMRALALIAPLLLFVLLTFILPIGDMLFRSVENSIVSETLPRTTAAAQSYDA
DSGEAPPEYAFDALAYDLVVAAEKKIHTRVGSRLNYENAGISSLFRKAGRRVDDIGEVYLDQFVDLDPAWEDPQALVALF
ADPSWAASAGGENEARFEYSSVAGEALPGATDAYAAWARTIQEEDQDSPTEEEPWAPVYMALYKDLAKNPDAASAFEGET
AARLAAAAAAIQSPDWETMSTRDAFIKIDDDWADPEVWRTIKTYSSKYTNGYFLNAADFQKGEDGPELRPELERIYGTLF
LRTMIMSLAITVSCIVLGYPVAWILANLPSRTANVLMILVLLPFWTSLLVRTSAWKVMLQQQGVINDVLVWLGLVADDAR
LVMINNQFGTIVAMTHILLPFMILPMYSVMQTINPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILAIGYYI
TPELVGGTKGVFISNRIAFHISQSLNWGLAAALGTILLVVVLVLYWAYDKIVGIDNVKLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=225, Percent_Identity=34.6666666666667, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787080, Length=230, Percent_Identity=33.9130434782609, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787713, Length=223, Percent_Identity=34.0807174887892, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 59384; Mature: 59253

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTDTGPVLAADGTPLKRSLARALRMQKMRALALIAPLLLFVLLTFILPIGDMLFRSV
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENSIVSETLPRTTAAAQSYDADSGEAPPEYAFDALAYDLVVAAEKKIHTRVGSRLNYENA
HHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GISSLFRKAGRRVDDIGEVYLDQFVDLDPAWEDPQALVALFADPSWAASAGGENEARFEY
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCEEH
SSVAGEALPGATDAYAAWARTIQEEDQDSPTEEEPWAPVYMALYKDLAKNPDAASAFEGE
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCH
TAARLAAAAAAIQSPDWETMSTRDAFIKIDDDWADPEVWRTIKTYSSKYTNGYFLNAADF
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCC
QKGEDGPELRPELERIYGTLFLRTMIMSLAITVSCIVLGYPVAWILANLPSRTANVLMIL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VLLPFWTSLLVRTSAWKVMLQQQGVINDVLVWLGLVADDARLVMINNQFGTIVAMTHILL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
PFMILPMYSVMQTINPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILAIGYY
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
ITPELVGGTKGVFISNRIAFHISQSLNWGLAAALGTILLVVVLVLYWAYDKIVGIDNVKL
HCHHHCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
G
C
>Mature Secondary Structure 
SDTTDTGPVLAADGTPLKRSLARALRMQKMRALALIAPLLLFVLLTFILPIGDMLFRSV
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENSIVSETLPRTTAAAQSYDADSGEAPPEYAFDALAYDLVVAAEKKIHTRVGSRLNYENA
HHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GISSLFRKAGRRVDDIGEVYLDQFVDLDPAWEDPQALVALFADPSWAASAGGENEARFEY
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCEEH
SSVAGEALPGATDAYAAWARTIQEEDQDSPTEEEPWAPVYMALYKDLAKNPDAASAFEGE
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCH
TAARLAAAAAAIQSPDWETMSTRDAFIKIDDDWADPEVWRTIKTYSSKYTNGYFLNAADF
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCC
QKGEDGPELRPELERIYGTLFLRTMIMSLAITVSCIVLGYPVAWILANLPSRTANVLMIL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VLLPFWTSLLVRTSAWKVMLQQQGVINDVLVWLGLVADDARLVMINNQFGTIVAMTHILL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
PFMILPMYSVMQTINPSYLRAAKSLGATNWTAFWRVYFPQSVPGIGAGSILVFILAIGYY
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
ITPELVGGTKGVFISNRIAFHISQSLNWGLAAALGTILLVVVLVLYWAYDKIVGIDNVKL
HCHHHCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]