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The map label for this gene is yagA [C]

Identifier: 99082810

GI number: 99082810

Start: 3135228

End: 3136025

Strand: Direct

Name: yagA [C]

Synonym: TM1040_2970

Alternate gene names: 99082810

Gene position: 3135228-3136025 (Clockwise)

Preceding gene: 99082809

Following gene: 99082817

Centisome position: 97.95

GC content: 59.4

Gene sequence:

>798_bases
ATGCGGGCGGATTGGCAGGTGTCGATCCGCCGGGCCTGCGAGGTGATCCGGTTCGATCCGAGAACCTATCGCTACAAATC
CCGCCGGCCTGGGCAGGCCGCTTTGGAACAGCGGATACGAGAGATTTGCCAGACGCGCGTGCGCTTCGGCTACAGGCGCG
TGCATGTGCTGCTGCGCCGGGAGGGCTGGGAGATCAACGCCAAGAAGACCTATCGCATTTACAAGGAGTTGGGTATGCAA
CTTCGGAGCAAGACACCTAAGCGCCGGGTGAAGGCCAAGCTGCGGGATGACCGCAAGGAGGCTGTCAGCCCCAATGACGT
CTGGGCTATGGACTTTGTTCATGATCAGCTGGCTACAGGCTGGAAACTTCGGGTTCTGACGGTCGTCGACACCTTCTCAC
GCTATGTCCCGGTGCTTGATGCTCGGTTCACATATCGCGGGGAGGACGTGGTCGCGACGCTTGAGCAAGTATGCAAGCGC
ACCGGCTACCCAGCCACCATCCGTGTTGACCAGGGCAGCGAGTTCATCTCCAAGGATATGGACCTTTGGGCCTATGCCAA
CGACGTGACATTGGACTTCTCCCGTCCGGGCAAACCTACCGACAACGCCTTCATCGAGGCGTTCAATGGCCGGTTCCGGG
CGGAGTGCCTGAACGCGCACTGGTTCATGAGCCTTGAAGATGCAGCCGAAAAGTTGGAGGCTTGGCGTAGAGACTACAAT
GAAGAACGCCCGCACGGGGCTATCGGGAACAAGGTCCCGGCAGACCTGATGAAATCAGTTCACGACACCAGCCCGTGA

Upstream 100 bases:

>100_bases
AGAAGATCGTCGCGGATCTCGCGCTCGACAAGGAAATGCTGCAGGACGTCATTAAGCGAAAGCTTTGAGGCCTGCCCGGA
AAAGGTCGCTGGTCGACGGG

Downstream 100 bases:

>100_bases
ACCTGATGGAACCGCGGAAATTCTAACAACGGCCGAGGGCACGTTGGGTAGCACATCAGAACCGCGGAAACTCTCATTAT
AAATGAGGGAGCCTCGGGGG

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRADWQVSIRRACEVIRFDPRTYRYKSRRPGQAALEQRIREICQTRVRFGYRRVHVLLRREGWEINAKKTYRIYKELGMQ
LRSKTPKRRVKAKLRDDRKEAVSPNDVWAMDFVHDQLATGWKLRVLTVVDTFSRYVPVLDARFTYRGEDVVATLEQVCKR
TGYPATIRVDQGSEFISKDMDLWAYANDVTLDFSRPGKPTDNAFIEAFNGRFRAECLNAHWFMSLEDAAEKLEAWRRDYN
EERPHGAIGNKVPADLMKSVHDTSP

Sequences:

>Translated_265_residues
MRADWQVSIRRACEVIRFDPRTYRYKSRRPGQAALEQRIREICQTRVRFGYRRVHVLLRREGWEINAKKTYRIYKELGMQ
LRSKTPKRRVKAKLRDDRKEAVSPNDVWAMDFVHDQLATGWKLRVLTVVDTFSRYVPVLDARFTYRGEDVVATLEQVCKR
TGYPATIRVDQGSEFISKDMDLWAYANDVTLDFSRPGKPTDNAFIEAFNGRFRAECLNAHWFMSLEDAAEKLEAWRRDYN
EERPHGAIGNKVPADLMKSVHDTSP
>Mature_265_residues
MRADWQVSIRRACEVIRFDPRTYRYKSRRPGQAALEQRIREICQTRVRFGYRRVHVLLRREGWEINAKKTYRIYKELGMQ
LRSKTPKRRVKAKLRDDRKEAVSPNDVWAMDFVHDQLATGWKLRVLTVVDTFSRYVPVLDARFTYRGEDVVATLEQVCKR
TGYPATIRVDQGSEFISKDMDLWAYANDVTLDFSRPGKPTDNAFIEAFNGRFRAECLNAHWFMSLEDAAEKLEAWRRDYN
EERPHGAIGNKVPADLMKSVHDTSP

Specific function: Unknown

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786462, Length=212, Percent_Identity=27.3584905660377, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789981, Length=232, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788405, Length=271, Percent_Identity=23.9852398523985, Blast_Score=61, Evalue=9e-11,
Organism=Escherichia coli, GI1787263, Length=271, Percent_Identity=23.9852398523985, Blast_Score=61, Evalue=9e-11,
Organism=Escherichia coli, GI1786753, Length=271, Percent_Identity=23.9852398523985, Blast_Score=61, Evalue=9e-11,
Organism=Escherichia coli, GI1786570, Length=271, Percent_Identity=23.9852398523985, Blast_Score=61, Evalue=9e-11,
Organism=Escherichia coli, GI1786490, Length=271, Percent_Identity=23.9852398523985, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31152; Mature: 31152

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRADWQVSIRRACEVIRFDPRTYRYKSRRPGQAALEQRIREICQTRVRFGYRRVHVLLRR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGWEINAKKTYRIYKELGMQLRSKTPKRRVKAKLRDDRKEAVSPNDVWAMDFVHDQLATG
CCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCC
WKLRVLTVVDTFSRYVPVLDARFTYRGEDVVATLEQVCKRTGYPATIRVDQGSEFISKDM
CEEEEHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCC
DLWAYANDVTLDFSRPGKPTDNAFIEAFNGRFRAECLNAHWFMSLEDAAEKLEAWRRDYN
HHEEEECCEEEECCCCCCCCCHHHHHHHCCCHHHHHHCCHHEEEHHHHHHHHHHHHHCCC
EERPHGAIGNKVPADLMKSVHDTSP
CCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MRADWQVSIRRACEVIRFDPRTYRYKSRRPGQAALEQRIREICQTRVRFGYRRVHVLLRR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGWEINAKKTYRIYKELGMQLRSKTPKRRVKAKLRDDRKEAVSPNDVWAMDFVHDQLATG
CCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCC
WKLRVLTVVDTFSRYVPVLDARFTYRGEDVVATLEQVCKRTGYPATIRVDQGSEFISKDM
CEEEEHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCC
DLWAYANDVTLDFSRPGKPTDNAFIEAFNGRFRAECLNAHWFMSLEDAAEKLEAWRRDYN
HHEEEECCEEEECCCCCCCCCHHHHHHHCCCHHHHHHCCHHEEEHHHHHHHHHHHHHCCC
EERPHGAIGNKVPADLMKSVHDTSP
CCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]