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The map label for this gene is psdht [H]

Identifier: 99082540

GI number: 99082540

Start: 2841928

End: 2842893

Strand: Direct

Name: psdht [H]

Synonym: TM1040_2700

Alternate gene names: 99082540

Gene position: 2841928-2842893 (Clockwise)

Preceding gene: 99082539

Following gene: 99082541

Centisome position: 88.78

GC content: 61.7

Gene sequence:

>966_bases
ATGAAGGACACCGCAATGTATATCCCAACCTACGAGGACATGCTGGCGGCGCATCAACGTATCGCGCCACACATCCACCG
CACCCCGGTGCGCCAGTCCGCCTACCTCAACGAACTGACCGGCGCGCAGCTGTTTTTCAAATGCGAAAACTTTCAGGAGC
CGGGGGCCTTCAAGGTGCGTGGCGCCACCAATGCAGTCTTTGGTCTGGACGCAGCACAGGCGGCCAAGGGCGTGGCGACG
CATTCCAGCGGCAACCATGCCTCCTGCCTGTCCTATGCGGCGATGCTGCGCGGCATTCCCTGCAACGTGGTGATGCCGCG
CACCGCGCCGCAGGCCAAAAAAGACACCGTGCGCCGCTATGGCGGGAAGATCACCGAATGTGAGCCCTCGACCACCTCGC
GCGAGGAGACATTTGCCCGCGTACAGGCCGAAACCGGTGGCGATTTTGTGCATCCCTACAACGATCCTCGGGTGATCGCT
GGCCAAGGCACCTGCGCCAAGGAGTTTGTCGAGCAGACCGACGGGCTTGATTGTGTGCTCGCGCCGATTGGCGGCGGCGG
CATGATTTCGGGCACCTGTCTGACGCTGTCGACGCTGGCACCCGAAACACGGATCATCGCCGCCGAGCCCGAGCAGGCTG
ATGACGCCTACCGCAGCTTCAAGGCGGGCTACATCATTGCCGATGATGCGCCCAGGACCATCGCCGACGGGCTGCTGGTG
CCGCTCAAGGATCTGACCTGGCATTTTGTGTCGAACCATGTGTCCGACATTCTGACCGCATCTGACCCTGAGATCATCGA
CGCGATGAAGCTCATCTGGAAGCACCTGCGCGTGGTGATGGAGCCCTCCTCTGCGGTGCCGCTCGCCACCATCCTGAAAA
ACCCCGAGACCTTTCGCGGCAAACGCGTTGGCATCGTTTTGACCGGTGGCAATGTCGATCTGGATCGTCTGCCCTGGACG
ATTTGA

Upstream 100 bases:

>100_bases
TGGTCGACCTCGGCCTTGATATCCGCCTCGGCTCCGGCGTTGCAGCGGCACAGGACTATTATCGCGGCCACACGGCGGCC
GCCCAAAAGGACGCCGCGTG

Downstream 100 bases:

>100_bases
GAACGATCTGACCTCAGCCAGACCCTGTTTGCCCTTATTTAGACTCTGATATGGGAGTAGCCCCAATGAATGCACCCGCA
AATTTCGACAGCCTCGAAGT

Product: pyridoxal-5'-phosphate-dependent enzyme, beta subunit

Products: @OXOB02.txt*Oxobutanoate!; NH3; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKDTAMYIPTYEDMLAAHQRIAPHIHRTPVRQSAYLNELTGAQLFFKCENFQEPGAFKVRGATNAVFGLDAAQAAKGVAT
HSSGNHASCLSYAAMLRGIPCNVVMPRTAPQAKKDTVRRYGGKITECEPSTTSREETFARVQAETGGDFVHPYNDPRVIA
GQGTCAKEFVEQTDGLDCVLAPIGGGGMISGTCLTLSTLAPETRIIAAEPEQADDAYRSFKAGYIIADDAPRTIADGLLV
PLKDLTWHFVSNHVSDILTASDPEIIDAMKLIWKHLRVVMEPSSAVPLATILKNPETFRGKRVGIVLTGGNVDLDRLPWT
I

Sequences:

>Translated_321_residues
MKDTAMYIPTYEDMLAAHQRIAPHIHRTPVRQSAYLNELTGAQLFFKCENFQEPGAFKVRGATNAVFGLDAAQAAKGVAT
HSSGNHASCLSYAAMLRGIPCNVVMPRTAPQAKKDTVRRYGGKITECEPSTTSREETFARVQAETGGDFVHPYNDPRVIA
GQGTCAKEFVEQTDGLDCVLAPIGGGGMISGTCLTLSTLAPETRIIAAEPEQADDAYRSFKAGYIIADDAPRTIADGLLV
PLKDLTWHFVSNHVSDILTASDPEIIDAMKLIWKHLRVVMEPSSAVPLATILKNPETFRGKRVGIVLTGGNVDLDRLPWT
I
>Mature_321_residues
MKDTAMYIPTYEDMLAAHQRIAPHIHRTPVRQSAYLNELTGAQLFFKCENFQEPGAFKVRGATNAVFGLDAAQAAKGVAT
HSSGNHASCLSYAAMLRGIPCNVVMPRTAPQAKKDTVRRYGGKITECEPSTTSREETFARVQAETGGDFVHPYNDPRVIA
GQGTCAKEFVEQTDGLDCVLAPIGGGGMISGTCLTLSTLAPETRIIAAEPEQADDAYRSFKAGYIIADDAPRTIADGLLV
PLKDLTWHFVSNHVSDILTASDPEIIDAMKLIWKHLRVVMEPSSAVPLATILKNPETFRGKRVGIVLTGGNVDLDRLPWT
I

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=320, Percent_Identity=45.3125, Blast_Score=269, Evalue=3e-72,
Organism=Escherichia coli, GI1789505, Length=309, Percent_Identity=33.0097087378641, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1790207, Length=298, Percent_Identity=32.2147651006711, Blast_Score=134, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17508781, Length=311, Percent_Identity=34.4051446945338, Blast_Score=180, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI71991565, Length=314, Percent_Identity=32.484076433121, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17537387, Length=308, Percent_Identity=30.8441558441558, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322631, Length=317, Percent_Identity=42.2712933753943, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6320930, Length=306, Percent_Identity=30.3921568627451, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319788, Length=285, Percent_Identity=21.4035087719298, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI21355833, Length=306, Percent_Identity=31.3725490196078, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24645328, Length=211, Percent_Identity=29.3838862559242, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19 [C]

Molecular weight: Translated: 34797; Mature: 34797

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTAMYIPTYEDMLAAHQRIAPHIHRTPVRQSAYLNELTGAQLFFKCENFQEPGAFKVR
CCCCEEECCCHHHHHHHHHHHCCCHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEC
GATNAVFGLDAAQAAKGVATHSSGNHASCLSYAAMLRGIPCNVVMPRTAPQAKKDTVRRY
CCCCEEEECCHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHH
GGKITECEPSTTSREETFARVQAETGGDFVHPYNDPRVIAGQGTCAKEFVEQTDGLDCVL
CCCCEECCCCCCCHHHHHHHEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEE
APIGGGGMISGTCLTLSTLAPETRIIAAEPEQADDAYRSFKAGYIIADDAPRTIADGLLV
EECCCCCEECCCEEEEECCCCCCEEEEECCCHHHHHHHHHHCCEEEECCCCHHHHCCCEE
PLKDLTWHFVSNHVSDILTASDPEIIDAMKLIWKHLRVVMEPSSAVPLATILKNPETFRG
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCHHCC
KRVGIVLTGGNVDLDRLPWTI
CEEEEEEECCCCCHHCCCCCC
>Mature Secondary Structure
MKDTAMYIPTYEDMLAAHQRIAPHIHRTPVRQSAYLNELTGAQLFFKCENFQEPGAFKVR
CCCCEEECCCHHHHHHHHHHHCCCHHCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEC
GATNAVFGLDAAQAAKGVATHSSGNHASCLSYAAMLRGIPCNVVMPRTAPQAKKDTVRRY
CCCCEEEECCHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHH
GGKITECEPSTTSREETFARVQAETGGDFVHPYNDPRVIAGQGTCAKEFVEQTDGLDCVL
CCCCEECCCCCCCHHHHHHHEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEE
APIGGGGMISGTCLTLSTLAPETRIIAAEPEQADDAYRSFKAGYIIADDAPRTIADGLLV
EECCCCCEECCCEEEEECCCCCCEEEEECCCHHHHHHHHHHCCEEEECCCCHHHHCCCEE
PLKDLTWHFVSNHVSDILTASDPEIIDAMKLIWKHLRVVMEPSSAVPLATILKNPETFRG
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCHHCC
KRVGIVLTGGNVDLDRLPWTI
CEEEEEEECCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: @THRE01.txt*L-threonine!; H2O [C]

Specific reaction: @THRE01.txt*L-threonine! + H2O = @OXOB02.txt*oxobutanoate! + NH3 + H2O [C]

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]