Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 99082522

GI number: 99082522

Start: 2819870

End: 2820934

Strand: Reverse

Name: ada [H]

Synonym: TM1040_2682

Alternate gene names: 99082522

Gene position: 2820934-2819870 (Counterclockwise)

Preceding gene: 99082523

Following gene: 99082518

Centisome position: 88.13

GC content: 64.69

Gene sequence:

>1065_bases
ATGATGATGTTTGACCTGCCCGATTCAAAAACCCTTTACGACGCGCTGGTGGCGCGGGATGCAAGCTATGACGGGCGCGC
CTATGTGGGCGTGTCCTCGACCGGGATCTTTTGTCGCCTGACCTGCCCCGCGCGCAAGCCGAAGTATGAGAATTGCCAGT
TTTTTGCCACTCCCGGTGCCTGCATCGAGGCTGGGTTTCGCGCCTGCAAGCGTTGTCACCCCCTGAATGCGGCGGCGGGG
AATGATCCGCTGGTGCGCCGCCTGCTGGAGGCGCTGGAGGCACGGCCCTTCTATCGCTGGCGCGAGGAGGATCTGGTGGC
CATGGGCGCAGACCCTTCTACCATCCGCCGGGCGTTCAAGCGTCAGTTTGGCATGACCTTTCTGGAAATGGCGCGGCAAC
GCCGCTTGCGCGAGGGGTTCACCACGCTCAGTGCCGGGCAGCCCGTGATCTCGGCGCAGCTTGAGGCCGGTTTTGAAAGC
CCCAGCGCTTTTCGCGCGGCCTTTGCCCGATTGGTCGGGATTGCGCCAGGGCAGTTTCGCAAGGATGCGCAGCTTTTGGC
GGATTGGATCGAGACCCCGCTGGGCGCGATGGTGGCGGTGGCCTGCCAGCACAAGCTGCATCTGTTGGAGTTTGCAGACC
GCAAGGCTTTGCCGCGCGAAGTGGCAAAGCTGCAAAAACGCCAGCCCGGCGGCATCGGCTTTGGCCGGCCCGAGGTGGTG
GATCAGGCGGCAGAGGAGTTGGGTGCCTATTTTACGGGGCGGGCAGCGCGGTTCGACACGCCGCTGGCCTATCACGGCAC
GGCCTTTGAGGCAGACGTCTGGCGTGCGCTCAGGGAGATCCCGGTGGGGCAGACCCGCAGCTACGGCGCCTTGGCGCAGA
GCCTTGGGCGACCGGGATCAAGCCGTGCGGTGGCGCGGGCCAACGGGGCCAACCAGATCGCGGTGATGATCCCCTGCCAC
AGGGTTCTCGGCGCGGATGGGGCATTGACAGGATATGGCGGTGGGCTCTGGCGCAAACAGAGGCTTATTGAAATCGAACG
CGACCTCAGCGCGGCCACTGGATGA

Upstream 100 bases:

>100_bases
CTCCCTTTGGGATGTATCGAAACCCTTGTCCCTGCTTTGAAATTGAATAGACGCCCGAATTCAGGCGGCAGCAACCAGAG
GCGGGTGTTAGAGTTGCGCC

Downstream 100 bases:

>100_bases
GCTTTCTGCCATTGAAGTAGCACAAGGGGATCCGACCGGCGCGCGCCCCTCGGCGGGCCCGTCCGGGCCCGCAAACCCTC
ACAAAAAAGGCGAAAGGGAT

Product: DNA-O6-methylguanine--protein-cysteine S-methyltransferase / transcriptional regulator Ada

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MMMFDLPDSKTLYDALVARDASYDGRAYVGVSSTGIFCRLTCPARKPKYENCQFFATPGACIEAGFRACKRCHPLNAAAG
NDPLVRRLLEALEARPFYRWREEDLVAMGADPSTIRRAFKRQFGMTFLEMARQRRLREGFTTLSAGQPVISAQLEAGFES
PSAFRAAFARLVGIAPGQFRKDAQLLADWIETPLGAMVAVACQHKLHLLEFADRKALPREVAKLQKRQPGGIGFGRPEVV
DQAAEELGAYFTGRAARFDTPLAYHGTAFEADVWRALREIPVGQTRSYGALAQSLGRPGSSRAVARANGANQIAVMIPCH
RVLGADGALTGYGGGLWRKQRLIEIERDLSAATG

Sequences:

>Translated_354_residues
MMMFDLPDSKTLYDALVARDASYDGRAYVGVSSTGIFCRLTCPARKPKYENCQFFATPGACIEAGFRACKRCHPLNAAAG
NDPLVRRLLEALEARPFYRWREEDLVAMGADPSTIRRAFKRQFGMTFLEMARQRRLREGFTTLSAGQPVISAQLEAGFES
PSAFRAAFARLVGIAPGQFRKDAQLLADWIETPLGAMVAVACQHKLHLLEFADRKALPREVAKLQKRQPGGIGFGRPEVV
DQAAEELGAYFTGRAARFDTPLAYHGTAFEADVWRALREIPVGQTRSYGALAQSLGRPGSSRAVARANGANQIAVMIPCH
RVLGADGALTGYGGGLWRKQRLIEIERDLSAATG
>Mature_354_residues
MMMFDLPDSKTLYDALVARDASYDGRAYVGVSSTGIFCRLTCPARKPKYENCQFFATPGACIEAGFRACKRCHPLNAAAG
NDPLVRRLLEALEARPFYRWREEDLVAMGADPSTIRRAFKRQFGMTFLEMARQRRLREGFTTLSAGQPVISAQLEAGFES
PSAFRAAFARLVGIAPGQFRKDAQLLADWIETPLGAMVAVACQHKLHLLEFADRKALPREVAKLQKRQPGGIGFGRPEVV
DQAAEELGAYFTGRAARFDTPLAYHGTAFEADVWRALREIPVGQTRSYGALAQSLGRPGSSRAVARANGANQIAVMIPCH
RVLGADGALTGYGGGLWRKQRLIEIERDLSAATG

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=177, Percent_Identity=29.3785310734463, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788542, Length=345, Percent_Identity=35.6521739130435, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1787596, Length=168, Percent_Identity=39.8809523809524, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=46.25, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=46.25, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=36.7816091954023, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137554, Length=125, Percent_Identity=36, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38773; Mature: 38773

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMFDLPDSKTLYDALVARDASYDGRAYVGVSSTGIFCRLTCPARKPKYENCQFFATPGA
CCEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEECCCH
CIEAGFRACKRCHPLNAAAGNDPLVRRLLEALEARPFYRWREEDLVAMGADPSTIRRAFK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCEEEECCCHHHHHHHHH
RQFGMTFLEMARQRRLREGFTTLSAGQPVISAQLEAGFESPSAFRAAFARLVGIAPGQFR
HHHCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHH
KDAQLLADWIETPLGAMVAVACQHKLHLLEFADRKALPREVAKLQKRQPGGIGFGRPEVV
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHH
DQAAEELGAYFTGRAARFDTPLAYHGTAFEADVWRALREIPVGQTRSYGALAQSLGRPGS
HHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
SRAVARANGANQIAVMIPCHRVLGADGALTGYGGGLWRKQRLIEIERDLSAATG
CCCEEECCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMMFDLPDSKTLYDALVARDASYDGRAYVGVSSTGIFCRLTCPARKPKYENCQFFATPGA
CCEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEECCCH
CIEAGFRACKRCHPLNAAAGNDPLVRRLLEALEARPFYRWREEDLVAMGADPSTIRRAFK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCEEEECCCHHHHHHHHH
RQFGMTFLEMARQRRLREGFTTLSAGQPVISAQLEAGFESPSAFRAAFARLVGIAPGQFR
HHHCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHH
KDAQLLADWIETPLGAMVAVACQHKLHLLEFADRKALPREVAKLQKRQPGGIGFGRPEVV
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHH
DQAAEELGAYFTGRAARFDTPLAYHGTAFEADVWRALREIPVGQTRSYGALAQSLGRPGS
HHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
SRAVARANGANQIAVMIPCHRVLGADGALTGYGGGLWRKQRLIEIERDLSAATG
CCCEEECCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]