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The map label for this gene is pmbA [H]

Identifier: 99082479

GI number: 99082479

Start: 2773042

End: 2774463

Strand: Direct

Name: pmbA [H]

Synonym: TM1040_2639

Alternate gene names: 99082479

Gene position: 2773042-2774463 (Clockwise)

Preceding gene: 99082478

Following gene: 99082480

Centisome position: 86.63

GC content: 66.6

Gene sequence:

>1422_bases
ATGGCGCAGAGCCCTTGCGATACGGCGGCGCTCTGGCTAGCTCTGGTTCAATGCCACCCATTTGCGAAGGTGCCCATGAC
ACAGACTCCTGAAACGCTTTGCCACGCCCTCCTTGATGCCGCCCAAAAGGCCGGGGCCGATTCTGCCGACGCCATGGCCG
CCGAGGGCAGCTCGCTCTCGATCGAGGTGCGCGAGGGCGCGCTGGAACATGCAGAGCGCTCCGAAGGGGTGGACATCGGG
CTGCGGGTCTTTGTCGGCCAGCGTCAGGCGCAGGTGTCCTCCTCCGATACCCGCCCCGAAACCCTGACCGCGATGGCCGA
ACGCGCCGTGGCCATGGCCAAAGAAGCGCCCGAAGATCCCTATGCCGGGCTTGCTGACCCCGCGCAGCTGGCCAAATCCT
GGGATCTCGACGCCCTTGAGATGGCCGACCCCAGCGCCGAGCCTGCGCCCGATCAACTGCAACAGGACGCGCTGGCCGCC
GAAAGCGCCTGCGCCGCCATCGACGGCATTTCTCAGGTCCAGTCCGCCGCGGCGGGCTATGGGCGTCATGACATCCACAT
GGCCGCGAGCAACGGGTTCTCCGGGGGCTATGCGCGCACCAGCCGCTCGATCTCCTGTGTGGGGATTGCGGGCACCGGCA
CCGGCATGGAGCGCGACTATGACGGCGACAGCCGCATCTATCAAACCGATCTGCGCAGCGCCGAAGAGATCGGGCGCACC
GCTGGCGAGCGCGCCATCGAACGTGTGAACGCCCGCCGCCCCAAAACTGGCGCCTATCCCGTGCTCTTTGACGAGCGGAT
CTCCTCATCCCTCATCGGGCATCTTCTGGGTGCCGCCAATGGCGCGTCGGTGGCGCGCGGCTCCTCGTGGCTCAAGGACA
GTCTCGGCGCGCAGATCCTGCCCGAGGCCTTCTCGGTCATCGAGGACCCCCTGCGCCCCCGCGTTTCAGGCTCGCGCCCC
TTTGATGGCGAAGGCCTGCCCACGCAGCGCCGCGCGATCGTCGACAAGGGCGTGCTGACCGGCTGGACCATGGATCTGGC
TTCGGCGCGCAAACTTGGCCTTGAGAGCACAGGCAACGCCGCGCGTGGCATCGGGTCGGTGCCGTCGCCCTCCAACTGGA
ACATCGCTCTGACCCAGGGGCAACAGACCCGCGAAGAGCTGCTGCGCGACATGGGCACCGGGCTTCTGGTCACCTCGATG
ATCGGCTCCACCATCAACCCCAACACGGGCGACTACTCGCGCGGCGCTTCGGGCTTCTGGGTGGAGAACGGCGAGATCCA
GTATCCGGTCAACGAGGTCACGATTGCCGGGAACCTCCTCGATATGCTGAAAACGCTGGTCGCCGCCAACGACGCCCGCA
CACATCTGTCGCGGGTGGTGCCATCGCTTCTGGTAGAGGGACTGACCCTTGCCGGAGAATGA

Upstream 100 bases:

>100_bases
GAAGGCCATGTGCTCGCCAACGGCCAGCGCCTGAAGTAACGCGCACAGGGCCACGCCCAAAGGATCAGAGGCCGCTCCTT
TGCCGGGGCGGCCTCTTTTT

Downstream 100 bases:

>100_bases
CCTCTCCCTTCTGATAGGGGCGGCCCAAAAGGCGGGCGAGATCGCGGGGCAATTCACCGGCACCGAGGCACAGAAATGGG
ACAAGCCCGATGGCGCGGGC

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAQSPCDTAALWLALVQCHPFAKVPMTQTPETLCHALLDAAQKAGADSADAMAAEGSSLSIEVREGALEHAERSEGVDIG
LRVFVGQRQAQVSSSDTRPETLTAMAERAVAMAKEAPEDPYAGLADPAQLAKSWDLDALEMADPSAEPAPDQLQQDALAA
ESACAAIDGISQVQSAAAGYGRHDIHMAASNGFSGGYARTSRSISCVGIAGTGTGMERDYDGDSRIYQTDLRSAEEIGRT
AGERAIERVNARRPKTGAYPVLFDERISSSLIGHLLGAANGASVARGSSWLKDSLGAQILPEAFSVIEDPLRPRVSGSRP
FDGEGLPTQRRAIVDKGVLTGWTMDLASARKLGLESTGNAARGIGSVPSPSNWNIALTQGQQTREELLRDMGTGLLVTSM
IGSTINPNTGDYSRGASGFWVENGEIQYPVNEVTIAGNLLDMLKTLVAANDARTHLSRVVPSLLVEGLTLAGE

Sequences:

>Translated_473_residues
MAQSPCDTAALWLALVQCHPFAKVPMTQTPETLCHALLDAAQKAGADSADAMAAEGSSLSIEVREGALEHAERSEGVDIG
LRVFVGQRQAQVSSSDTRPETLTAMAERAVAMAKEAPEDPYAGLADPAQLAKSWDLDALEMADPSAEPAPDQLQQDALAA
ESACAAIDGISQVQSAAAGYGRHDIHMAASNGFSGGYARTSRSISCVGIAGTGTGMERDYDGDSRIYQTDLRSAEEIGRT
AGERAIERVNARRPKTGAYPVLFDERISSSLIGHLLGAANGASVARGSSWLKDSLGAQILPEAFSVIEDPLRPRVSGSRP
FDGEGLPTQRRAIVDKGVLTGWTMDLASARKLGLESTGNAARGIGSVPSPSNWNIALTQGQQTREELLRDMGTGLLVTSM
IGSTINPNTGDYSRGASGFWVENGEIQYPVNEVTIAGNLLDMLKTLVAANDARTHLSRVVPSLLVEGLTLAGE
>Mature_472_residues
AQSPCDTAALWLALVQCHPFAKVPMTQTPETLCHALLDAAQKAGADSADAMAAEGSSLSIEVREGALEHAERSEGVDIGL
RVFVGQRQAQVSSSDTRPETLTAMAERAVAMAKEAPEDPYAGLADPAQLAKSWDLDALEMADPSAEPAPDQLQQDALAAE
SACAAIDGISQVQSAAAGYGRHDIHMAASNGFSGGYARTSRSISCVGIAGTGTGMERDYDGDSRIYQTDLRSAEEIGRTA
GERAIERVNARRPKTGAYPVLFDERISSSLIGHLLGAANGASVARGSSWLKDSLGAQILPEAFSVIEDPLRPRVSGSRPF
DGEGLPTQRRAIVDKGVLTGWTMDLASARKLGLESTGNAARGIGSVPSPSNWNIALTQGQQTREELLRDMGTGLLVTSMI
GSTINPNTGDYSRGASGFWVENGEIQYPVNEVTIAGNLLDMLKTLVAANDARTHLSRVVPSLLVEGLTLAGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=435, Percent_Identity=38.3908045977011, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1789640, Length=171, Percent_Identity=25.7309941520468, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49729; Mature: 49598

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSPCDTAALWLALVQCHPFAKVPMTQTPETLCHALLDAAQKAGADSADAMAAEGSSLS
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEE
IEVREGALEHAERSEGVDIGLRVFVGQRQAQVSSSDTRPETLTAMAERAVAMAKEAPEDP
EEEHHHHHHHHHHCCCCCEEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
YAGLADPAQLAKSWDLDALEMADPSAEPAPDQLQQDALAAESACAAIDGISQVQSAAAGY
CCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRHDIHMAASNGFSGGYARTSRSISCVGIAGTGTGMERDYDGDSRIYQTDLRSAEEIGRT
CCCEEEEEECCCCCCCHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGERAIERVNARRPKTGAYPVLFDERISSSLIGHLLGAANGASVARGSSWLKDSLGAQIL
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHHCHHHHHHHCCHHHH
PEAFSVIEDPLRPRVSGSRPFDGEGLPTQRRAIVDKGVLTGWTMDLASARKLGLESTGNA
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
ARGIGSVPSPSNWNIALTQGQQTREELLRDMGTGLLVTSMIGSTINPNTGDYSRGASGFW
HHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEE
VENGEIQYPVNEVTIAGNLLDMLKTLVAANDARTHLSRVVPSLLVEGLTLAGE
EECCEEECCHHHEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQSPCDTAALWLALVQCHPFAKVPMTQTPETLCHALLDAAQKAGADSADAMAAEGSSLS
CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEE
IEVREGALEHAERSEGVDIGLRVFVGQRQAQVSSSDTRPETLTAMAERAVAMAKEAPEDP
EEEHHHHHHHHHHCCCCCEEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
YAGLADPAQLAKSWDLDALEMADPSAEPAPDQLQQDALAAESACAAIDGISQVQSAAAGY
CCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRHDIHMAASNGFSGGYARTSRSISCVGIAGTGTGMERDYDGDSRIYQTDLRSAEEIGRT
CCCEEEEEECCCCCCCHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGERAIERVNARRPKTGAYPVLFDERISSSLIGHLLGAANGASVARGSSWLKDSLGAQIL
HHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHHCHHHHHHHCCHHHH
PEAFSVIEDPLRPRVSGSRPFDGEGLPTQRRAIVDKGVLTGWTMDLASARKLGLESTGNA
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
ARGIGSVPSPSNWNIALTQGQQTREELLRDMGTGLLVTSMIGSTINPNTGDYSRGASGFW
HHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCEE
VENGEIQYPVNEVTIAGNLLDMLKTLVAANDARTHLSRVVPSLLVEGLTLAGE
EECCEEECCHHHEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]