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The map label for this gene is yngF [H]

Identifier: 99082476

GI number: 99082476

Start: 2769971

End: 2770585

Strand: Reverse

Name: yngF [H]

Synonym: TM1040_2636

Alternate gene names: 99082476

Gene position: 2770585-2769971 (Counterclockwise)

Preceding gene: 99082477

Following gene: 99082471

Centisome position: 86.56

GC content: 67.15

Gene sequence:

>615_bases
ATGATCGACCATCATCAGGATTCGCGCGGGCTGCTGACCTTGCGGCTCAACCGGCCCGAGAAGGCCAATGCGCTGACGCC
CGAGATGCTGGCGCAGATGATCGAGATCCTGCGCACCGCAACAGAGGCCCGCGCGCTGATCCTGACCGGCACCGGCAAGG
TGTTCAGCGCAGGCGCGGATCTGGACGCGGCAAAGGCGGGGCTTGCGACCTCGCCTTTGTGGGAAGAGCTCTCGGGCGCG
CTTGCGGCGCTCCCCTGTCTCAAGGTTGCGGCCCTCAACGGAACGCTGGCGGGCGGGGCCAATGGCATGGCGCTGGCCTG
TGATCTGCGCATCGCGGTGCCCACGGCCAAGGTGTTCTATCCGGTGATGCAGCTTGGCTATTTGCCGCAGCCCTCGGATG
CGAAGCGGATGCGGGCGCTTGTAGGGCCTGCGCGCACCAAGCTGATCCTTGCGGCGGGCCAGAAGATCACCGCCGAGGAG
GCCTATGGCTTTGGGCTGATCGACCGGATCGTGGCGCCCGAAGAGCTGATGGCAGAGGCTGAGGCGCTGGTTGCGCCCGC
GCTTGCAGCAGAGCCTGATGTGGCGCGCGCGATCCTGCGCGCTGCTGACGCCTGA

Upstream 100 bases:

>100_bases
CGCGCACGCTGATGTGGATGCTGAGCGCGGATGCGGATGAATTTGTGGGCACCGAGATCGCGCTGCGCGATGAAGGCATC
CGCAAACGGGTTGGTCTCAT

Downstream 100 bases:

>100_bases
GTCCGGGCGAGGCCTGCCCCCAACAGCGCGACGCCTGCCCCCCCAAAAACTAAAAAACGCACCCCCGAGGGAGCGCGTTT
TTTTAAAATCTCAGCGCGGG

Product: enoyl-CoA hydratase/isomerase

Products: crotonoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MIDHHQDSRGLLTLRLNRPEKANALTPEMLAQMIEILRTATEARALILTGTGKVFSAGADLDAAKAGLATSPLWEELSGA
LAALPCLKVAALNGTLAGGANGMALACDLRIAVPTAKVFYPVMQLGYLPQPSDAKRMRALVGPARTKLILAAGQKITAEE
AYGFGLIDRIVAPEELMAEAEALVAPALAAEPDVARAILRAADA

Sequences:

>Translated_204_residues
MIDHHQDSRGLLTLRLNRPEKANALTPEMLAQMIEILRTATEARALILTGTGKVFSAGADLDAAKAGLATSPLWEELSGA
LAALPCLKVAALNGTLAGGANGMALACDLRIAVPTAKVFYPVMQLGYLPQPSDAKRMRALVGPARTKLILAAGQKITAEE
AYGFGLIDRIVAPEELMAEAEALVAPALAAEPDVARAILRAADA
>Mature_204_residues
MIDHHQDSRGLLTLRLNRPEKANALTPEMLAQMIEILRTATEARALILTGTGKVFSAGADLDAAKAGLATSPLWEELSGA
LAALPCLKVAALNGTLAGGANGMALACDLRIAVPTAKVFYPVMQLGYLPQPSDAKRMRALVGPARTKLILAAGQKITAEE
AYGFGLIDRIVAPEELMAEAEALVAPALAAEPDVARAILRAADA

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI157694516, Length=212, Percent_Identity=25.4716981132075, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI213417737, Length=212, Percent_Identity=25.4716981132075, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI157694520, Length=183, Percent_Identity=24.5901639344262, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI70995211, Length=222, Percent_Identity=26.1261261261261, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI194097323, Length=177, Percent_Identity=28.8135593220339, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI260274832, Length=181, Percent_Identity=27.0718232044199, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI45643119, Length=181, Percent_Identity=27.0718232044199, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI260275230, Length=181, Percent_Identity=27.0718232044199, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1787660, Length=206, Percent_Identity=32.5242718446602, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI221142681, Length=199, Percent_Identity=32.1608040201005, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1787659, Length=179, Percent_Identity=32.9608938547486, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790281, Length=196, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1788682, Length=173, Percent_Identity=30.0578034682081, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=188, Percent_Identity=27.6595744680851, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=194, Percent_Identity=28.3505154639175, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=203, Percent_Identity=26.6009852216749, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20129971, Length=187, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653477, Length=187, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653139, Length=177, Percent_Identity=28.8135593220339, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 21289; Mature: 21289

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDHHQDSRGLLTLRLNRPEKANALTPEMLAQMIEILRTATEARALILTGTGKVFSAGAD
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCC
LDAAKAGLATSPLWEELSGALAALPCLKVAALNGTLAGGANGMALACDLRIAVPTAKVFY
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCHHHHHH
PVMQLGYLPQPSDAKRMRALVGPARTKLILAAGQKITAEEAYGFGLIDRIVAPEELMAEA
HHHHHCCCCCCCHHHHHHHHHCHHHHEEEEECCCEECHHHHCCCHHHHHHCCCHHHHHHH
EALVAPALAAEPDVARAILRAADA
HHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIDHHQDSRGLLTLRLNRPEKANALTPEMLAQMIEILRTATEARALILTGTGKVFSAGAD
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCC
LDAAKAGLATSPLWEELSGALAALPCLKVAALNGTLAGGANGMALACDLRIAVPTAKVFY
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCHHHHHH
PVMQLGYLPQPSDAKRMRALVGPARTKLILAAGQKITAEEAYGFGLIDRIVAPEELMAEA
HHHHHCCCCCCCHHHHHHHHHCHHHHEEEEECCCEECHHHHCCCHHHHHHCCCHHHHHHH
EALVAPALAAEPDVARAILRAADA
HHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]