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The map label for this gene is amtB [C]

Identifier: 99082470

GI number: 99082470

Start: 2764013

End: 2765335

Strand: Direct

Name: amtB [C]

Synonym: TM1040_2630

Alternate gene names: 99082470

Gene position: 2764013-2765335 (Clockwise)

Preceding gene: 99082469

Following gene: 99082472

Centisome position: 86.35

GC content: 61.9

Gene sequence:

>1323_bases
ATGAAGAAACTGACAGTGACACTCGCCGCCACGGCGCTGGCGACCCTGCCGGGTCTGGCCTTCGCGCAGGAAGCCGCGGC
CGAGACACCAGACATCAACCCTTATATCTTCACCACGCTCCTGTTCCTGATCGGCGGTTTCCTGGTGTTCTGGATGGCCG
CAGGCTTTGCCATGCTCGAAGCAGGGCTCGTGCGCTCCAAGAACGTGACCATGCAGCTCACCAAGAACGTGGCGCTCTTC
TCGATCGCCGCGATCATGTACTGGCTCGTGGGCTTCAACCTGATGTATCCGGGTGATGGCTGGATCATCCCGGGCGTGAT
GGGGCCGCTCTTTGCCACCACCTCGCTGGAGCCTGTGGGCCTCGGTGCCGACGCCGCAGCGCTGGATTATGCTTCCGTGG
GTTCGGACTTTTTCTTCCAGCTGATGTTCTGCGCCACCACCGCCTCCATCGTATCCGGCACGCTGGCCGAGCGCATCAAG
CTGTGGCCCTTCCTGATCTTCGTCGCGATCCTGACCGGCGTGATCTACCCGATCGAGGCCTCCTGGGTCTGGGGTGAGGG
CTTCCTCGATGGGCTAGGATTCTCTGACTTTGCGGGCTCCACCCTGGTGCATGCTGCTGGTGGCTTTGCGGCCCTCGCCG
GTGCCCTGATCCTCGGTCCGCGGATCGGCAAATACAAAGATGGCCGCACCATTCCGATGCCCGGCTCCAACCTCGCACTG
GCCACTCTGGGTACCTTCATCCTGTGGCTCGGCTGGTTTGGCTTCAACGGTGGTTCGCAGCTGGCGATGGGCACCGTGGG
CGATGTGACCGACGTGTCGCGCATCTTTGCCAACACCAACATGGCTGCCGCAGCCGGTGCTGTGACCGCGCTGATCCTGA
CCCAGCTTCTCTTCAAGAAGCCCGACCTCACCATGATCCTCAACGGCGCACTGGCCGGTCTGGTCTCCATCACCGCAGGT
CCGCTTGACCCGACCCTCTTTGGAGCGCTCCTGATCGGTGCCGTGGGTGGCGTGATCGTTGTCTTCACCGTGCCGATGCT
CGACAAGATGAAGATCGACGACGTTGTGGGTGCGATCCCGGTTCACCTGCTGGCCGGTCTCTGGGGGACGTTTGCGGTTC
CCTTCTACACCGAAGGCACCTCCTTCATCACTCAGATCGTCGGCTTTGCCACCATCGGTATCTTCACCTTCGTGGTCTCC
GGTGTGGTCTGGATGATCCTGAAGGCCACCATGGGCATCCGCGTCTCCGAGGAGGACGAGATCAACGGTCTCGACATGGC
GGAGCTCGGCATGGAAGCCTACCCGGAGTTCAAAGGCAGCTAA

Upstream 100 bases:

>100_bases
AGATCTTCGTGCTGGATGTGGCGCAGGCCGTGCGCGTGCGCACCGGGGAAACCAATCAAGACGCGCTCTAACGCGCCTTT
CGCAAAGGAAAGCCAAAGAA

Downstream 100 bases:

>100_bases
GCCTTCGCTCCATAGAACAAAAGAAAACGCGGCTCCTCGGGGCCGCGTTTTTTTATTCATTTATTCAGAATGTTAGCGCA
CCGGCTCGAAGGTCGCATTG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKKLTVTLAATALATLPGLAFAQEAAAETPDINPYIFTTLLFLIGGFLVFWMAAGFAMLEAGLVRSKNVTMQLTKNVALF
SIAAIMYWLVGFNLMYPGDGWIIPGVMGPLFATTSLEPVGLGADAAALDYASVGSDFFFQLMFCATTASIVSGTLAERIK
LWPFLIFVAILTGVIYPIEASWVWGEGFLDGLGFSDFAGSTLVHAAGGFAALAGALILGPRIGKYKDGRTIPMPGSNLAL
ATLGTFILWLGWFGFNGGSQLAMGTVGDVTDVSRIFANTNMAAAAGAVTALILTQLLFKKPDLTMILNGALAGLVSITAG
PLDPTLFGALLIGAVGGVIVVFTVPMLDKMKIDDVVGAIPVHLLAGLWGTFAVPFYTEGTSFITQIVGFATIGIFTFVVS
GVVWMILKATMGIRVSEEDEINGLDMAELGMEAYPEFKGS

Sequences:

>Translated_440_residues
MKKLTVTLAATALATLPGLAFAQEAAAETPDINPYIFTTLLFLIGGFLVFWMAAGFAMLEAGLVRSKNVTMQLTKNVALF
SIAAIMYWLVGFNLMYPGDGWIIPGVMGPLFATTSLEPVGLGADAAALDYASVGSDFFFQLMFCATTASIVSGTLAERIK
LWPFLIFVAILTGVIYPIEASWVWGEGFLDGLGFSDFAGSTLVHAAGGFAALAGALILGPRIGKYKDGRTIPMPGSNLAL
ATLGTFILWLGWFGFNGGSQLAMGTVGDVTDVSRIFANTNMAAAAGAVTALILTQLLFKKPDLTMILNGALAGLVSITAG
PLDPTLFGALLIGAVGGVIVVFTVPMLDKMKIDDVVGAIPVHLLAGLWGTFAVPFYTEGTSFITQIVGFATIGIFTFVVS
GVVWMILKATMGIRVSEEDEINGLDMAELGMEAYPEFKGS
>Mature_440_residues
MKKLTVTLAATALATLPGLAFAQEAAAETPDINPYIFTTLLFLIGGFLVFWMAAGFAMLEAGLVRSKNVTMQLTKNVALF
SIAAIMYWLVGFNLMYPGDGWIIPGVMGPLFATTSLEPVGLGADAAALDYASVGSDFFFQLMFCATTASIVSGTLAERIK
LWPFLIFVAILTGVIYPIEASWVWGEGFLDGLGFSDFAGSTLVHAAGGFAALAGALILGPRIGKYKDGRTIPMPGSNLAL
ATLGTFILWLGWFGFNGGSQLAMGTVGDVTDVSRIFANTNMAAAAGAVTALILTQLLFKKPDLTMILNGALAGLVSITAG
PLDPTLFGALLIGAVGGVIVVFTVPMLDKMKIDDVVGAIPVHLLAGLWGTFAVPFYTEGTSFITQIVGFATIGIFTFVVS
GVVWMILKATMGIRVSEEDEINGLDMAELGMEAYPEFKGS

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=450, Percent_Identity=32.2222222222222, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32564042, Length=431, Percent_Identity=31.7865429234339, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17550182, Length=424, Percent_Identity=32.7830188679245, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17550180, Length=443, Percent_Identity=32.7313769751693, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI133931100, Length=419, Percent_Identity=30.5489260143198, Blast_Score=171, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6325396, Length=430, Percent_Identity=28.6046511627907, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321559, Length=433, Percent_Identity=27.9445727482679, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=418, Percent_Identity=28.2296650717703, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078314, Length=413, Percent_Identity=34.3825665859564, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46230; Mature: 46230

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTVTLAATALATLPGLAFAQEAAAETPDINPYIFTTLLFLIGGFLVFWMAAGFAMLE
CCEEEEEHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLVRSKNVTMQLTKNVALFSIAAIMYWLVGFNLMYPGDGWIIPGVMGPLFATTSLEPVG
HHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCEEECCCCCEECCCHHHHHHHCCCCCCCC
LGADAAALDYASVGSDFFFQLMFCATTASIVSGTLAERIKLWPFLIFVAILTGVIYPIEA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
SWVWGEGFLDGLGFSDFAGSTLVHAAGGFAALAGALILGPRIGKYKDGRTIPMPGSNLAL
CEEECCHHHCCCCCCHHCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
ATLGTFILWLGWFGFNGGSQLAMGTVGDVTDVSRIFANTNMAAAAGAVTALILTQLLFKK
HHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
PDLTMILNGALAGLVSITAGPLDPTLFGALLIGAVGGVIVVFTVPMLDKMKIDDVVGAIP
CCHHEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEHHHHCCCCHHHHHHHHHHHH
VHLLAGLWGTFAVPFYTEGTSFITQIVGFATIGIFTFVVSGVVWMILKATMGIRVSEEDE
HHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
INGLDMAELGMEAYPEFKGS
CCCCCHHHHCHHHCCCCCCC
>Mature Secondary Structure
MKKLTVTLAATALATLPGLAFAQEAAAETPDINPYIFTTLLFLIGGFLVFWMAAGFAMLE
CCEEEEEHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLVRSKNVTMQLTKNVALFSIAAIMYWLVGFNLMYPGDGWIIPGVMGPLFATTSLEPVG
HHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCEEECCCCCEECCCHHHHHHHCCCCCCCC
LGADAAALDYASVGSDFFFQLMFCATTASIVSGTLAERIKLWPFLIFVAILTGVIYPIEA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
SWVWGEGFLDGLGFSDFAGSTLVHAAGGFAALAGALILGPRIGKYKDGRTIPMPGSNLAL
CEEECCHHHCCCCCCHHCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
ATLGTFILWLGWFGFNGGSQLAMGTVGDVTDVSRIFANTNMAAAAGAVTALILTQLLFKK
HHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
PDLTMILNGALAGLVSITAGPLDPTLFGALLIGAVGGVIVVFTVPMLDKMKIDDVVGAIP
CCHHEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEHHHHCCCCHHHHHHHHHHHH
VHLLAGLWGTFAVPFYTEGTSFITQIVGFATIGIFTFVVSGVVWMILKATMGIRVSEEDE
HHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCC
INGLDMAELGMEAYPEFKGS
CCCCCHHHHCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]