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The map label for this gene is pbpG [H]

Identifier: 99082468

GI number: 99082468

Start: 2761148

End: 2763355

Strand: Reverse

Name: pbpG [H]

Synonym: TM1040_2628

Alternate gene names: 99082468

Gene position: 2763355-2761148 (Counterclockwise)

Preceding gene: 99082471

Following gene: 99082460

Centisome position: 86.33

GC content: 64.49

Gene sequence:

>2208_bases
ATGACTCAGGGGCGCAATTCTGGGCGCGGCACTGGACGCGGGCGTGGCCCGCTGGTTGCAGAGAAACGCAATTTGGGCGG
CAATCAGCGTAAACCGGCCAAAAAAGCGCCAAGGAAGCGCGCCCCACAAAAGGCCAAACGCGGCTGGAGCCTGTTTGGAC
GGCGCAAGACCGCGCGCCCGCAGCGCAGACGTGGACTCCTGGGCTGGCTGTTGCTGCCTTTTCAGATCGTGGCACGGGTG
ATCTGGGCGGTGAGCTGGCGCATGGGGGTTGTCGGCGGTCTCTTTCTGGCCGGGGCGGTCGCCTTTCACTACACCAAGCT
GCCCGAGTATCAGGCGCTGCTGGATGGGCGGCATCAGGGCTCGGTGACGCTGCTCGATCGCAACGGCAAGACATTTGCAT
GGCGCGGCGATCAGTTTGGCGGCGTGGTTACGGCGGATACCGTCTCGCCGCATCTGAAGAACGCGATCGTCGCCACCGAG
GACAAACGGTTTTACCGCCATTTCGGGATTTCGCCGCGCGGCATCGCCAGCGCGGTGCGCATCAACCTCAGCGAAGGGCG
TGGCCCGCTGTCGGGGCATGGGGGCTCTACGATCACCCAGCAGACCGCCAAGCTGATGTGCCTTGGGGTGGAATATGATC
CCGACATCTGGGAGAGCGAAGCCGCCTATGAGAGCGACTGCCGCCAGTCGTCGCTCTGGCGCAAGGGGAAGGAAGCGATC
TTTGCCCTCGCGATGGAGGTGAAATACACCAAGAACGAGATCCTCTCCATCTATATGAACCGCGCCTATATGGGCGGTGG
GGCACATGGGGTGGAGGCCGCTGCGCAGCGCTATTTCGGCAAATCCGCAAATCAGGTGAACCCGCCGGAGGCGGCGATGC
TGGCGGGGCTCTTGACCGCGCCGTCGACGCTGGCGCCCACCAACAATATCGAACGCTCACGCAATCGCGCGGCCACTGTG
CTGCGGCTGATGGAAGAACAGGGCTACCTCAGCCCCAAGGAACGCCAGATGGCCGCCGCCAATCCCGCGGAGCTGAGCGA
GGCGGCGGCAGCGCGCGCGGGGGGCTATTTTGCCGATTGGATCATGGACACGGTGCCGTCCTTTCTGGGCGATCAGACCA
CCGAAGATGTGGTGATCCGCACCACGCTTGATCAACGGCTGCAACAGGCCGCCGAGGATGCGGTGAACTATGTGTTTGAA
AACAAGGTGCGTGCAGGCTCCAAGGCGCAGACCGCTGTGGTCGTGATGAGCGCCGATGGCGCGGTGCGGGCCATGGTGGG
CGGGCGCAAGGTGCGTGCCTCGGGTGTGTTCAACCGCGCCACGCAGGCCAAGCGCCAGACCGGTTCGGCGTTCAAACCCT
TTGTCTATGCCGCCGCGCTGGAGCTTGGCTATTCGCCCTATGACCGGGTGCTGGATGCGGAATATTGTCTCGATATTCCC
GGCTCCGGCCCGTGGTGCCCACAGAACTACACTCGCAAGTTCTATGGCGAGGTGACATTGGCGCGCGCGCTTCGGGACTC
GCTCAATGTGCCCGCGGTCAAAGTCTCCGAGGCGGTGGGCCGCGACAAGGTGCGTCTGGTGGCAGGCGAATTCGGCATCG
AAAGCGATCTTGCCGATGGGCCCTCGCTGGCGCTGGGCGTCTCTGAAAGCACGCTCCTGGAAATGACCGGCGCCTATGCG
GGCATCCTGAACGGTGGCTCCTCCGTGACACCCTACGGGGTGGAGGCGCTGACGCTGGTCGGCGATTCAGAGCCATTGAT
GGGCGCGTCCGGCGGCATGGGGGAGCGGGTGATCCGCCCCGTCGCGGCGCAGGAGCTGATCTGGATGATGGAAAAAGTCA
TCGCCGAGGGCACCGGGCAACGCGCGCAGATCCCCGGCTGGCAGGCGGCAGGGAAATCCGGCACCACCTCTTCGGCCAAG
GATGCATGGTTTATTGGCTTTACCGCCGATTACGTCGCCGGGGTCTGGATGGGCTATGATGACAACACGCCGCTCACCGG
TGTCACCGGCGGCGGTTTGCCCGCAGAGATCTGGCGCGAGACGATGGTGCGGGTCCACGATGGGTTGGCCCCCCAGCCCC
TGCCAATGGCAGCGCCCGAGCCGATCGTGGTGCCCGAGCCTCAGCCGCAGCGGCGCAATGGGGGCGGCTTTGGCCGGGAG
CTTGAGCGCACCTTGAATGGCGTGTTGCAGGACATCTTTGGCAATTGA

Upstream 100 bases:

>100_bases
CTCTCTACATCAGGGGCCGTAGGGGCTATTCTGTGCAAAACAAGGAAGGCGTAAGCCCGCCGAGACCTAAAAGACGAGCA
GTCAGAACAGGATTTTCAGT

Downstream 100 bases:

>100_bases
CCGGATACTTTCTGGCCTGAAAGCCTTGGCCCGCCTACATTGGCCTGCGCGGACTGGCCCGAGCGCATTGTGGGCTCAGG
CCAGTGCCAGTAGCTGTCTG

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 735; Mature: 734

Protein sequence:

>735_residues
MTQGRNSGRGTGRGRGPLVAEKRNLGGNQRKPAKKAPRKRAPQKAKRGWSLFGRRKTARPQRRRGLLGWLLLPFQIVARV
IWAVSWRMGVVGGLFLAGAVAFHYTKLPEYQALLDGRHQGSVTLLDRNGKTFAWRGDQFGGVVTADTVSPHLKNAIVATE
DKRFYRHFGISPRGIASAVRINLSEGRGPLSGHGGSTITQQTAKLMCLGVEYDPDIWESEAAYESDCRQSSLWRKGKEAI
FALAMEVKYTKNEILSIYMNRAYMGGGAHGVEAAAQRYFGKSANQVNPPEAAMLAGLLTAPSTLAPTNNIERSRNRAATV
LRLMEEQGYLSPKERQMAAANPAELSEAAAARAGGYFADWIMDTVPSFLGDQTTEDVVIRTTLDQRLQQAAEDAVNYVFE
NKVRAGSKAQTAVVVMSADGAVRAMVGGRKVRASGVFNRATQAKRQTGSAFKPFVYAAALELGYSPYDRVLDAEYCLDIP
GSGPWCPQNYTRKFYGEVTLARALRDSLNVPAVKVSEAVGRDKVRLVAGEFGIESDLADGPSLALGVSESTLLEMTGAYA
GILNGGSSVTPYGVEALTLVGDSEPLMGASGGMGERVIRPVAAQELIWMMEKVIAEGTGQRAQIPGWQAAGKSGTTSSAK
DAWFIGFTADYVAGVWMGYDDNTPLTGVTGGGLPAEIWRETMVRVHDGLAPQPLPMAAPEPIVVPEPQPQRRNGGGFGRE
LERTLNGVLQDIFGN

Sequences:

>Translated_735_residues
MTQGRNSGRGTGRGRGPLVAEKRNLGGNQRKPAKKAPRKRAPQKAKRGWSLFGRRKTARPQRRRGLLGWLLLPFQIVARV
IWAVSWRMGVVGGLFLAGAVAFHYTKLPEYQALLDGRHQGSVTLLDRNGKTFAWRGDQFGGVVTADTVSPHLKNAIVATE
DKRFYRHFGISPRGIASAVRINLSEGRGPLSGHGGSTITQQTAKLMCLGVEYDPDIWESEAAYESDCRQSSLWRKGKEAI
FALAMEVKYTKNEILSIYMNRAYMGGGAHGVEAAAQRYFGKSANQVNPPEAAMLAGLLTAPSTLAPTNNIERSRNRAATV
LRLMEEQGYLSPKERQMAAANPAELSEAAAARAGGYFADWIMDTVPSFLGDQTTEDVVIRTTLDQRLQQAAEDAVNYVFE
NKVRAGSKAQTAVVVMSADGAVRAMVGGRKVRASGVFNRATQAKRQTGSAFKPFVYAAALELGYSPYDRVLDAEYCLDIP
GSGPWCPQNYTRKFYGEVTLARALRDSLNVPAVKVSEAVGRDKVRLVAGEFGIESDLADGPSLALGVSESTLLEMTGAYA
GILNGGSSVTPYGVEALTLVGDSEPLMGASGGMGERVIRPVAAQELIWMMEKVIAEGTGQRAQIPGWQAAGKSGTTSSAK
DAWFIGFTADYVAGVWMGYDDNTPLTGVTGGGLPAEIWRETMVRVHDGLAPQPLPMAAPEPIVVPEPQPQRRNGGGFGRE
LERTLNGVLQDIFGN
>Mature_734_residues
TQGRNSGRGTGRGRGPLVAEKRNLGGNQRKPAKKAPRKRAPQKAKRGWSLFGRRKTARPQRRRGLLGWLLLPFQIVARVI
WAVSWRMGVVGGLFLAGAVAFHYTKLPEYQALLDGRHQGSVTLLDRNGKTFAWRGDQFGGVVTADTVSPHLKNAIVATED
KRFYRHFGISPRGIASAVRINLSEGRGPLSGHGGSTITQQTAKLMCLGVEYDPDIWESEAAYESDCRQSSLWRKGKEAIF
ALAMEVKYTKNEILSIYMNRAYMGGGAHGVEAAAQRYFGKSANQVNPPEAAMLAGLLTAPSTLAPTNNIERSRNRAATVL
RLMEEQGYLSPKERQMAAANPAELSEAAAARAGGYFADWIMDTVPSFLGDQTTEDVVIRTTLDQRLQQAAEDAVNYVFEN
KVRAGSKAQTAVVVMSADGAVRAMVGGRKVRASGVFNRATQAKRQTGSAFKPFVYAAALELGYSPYDRVLDAEYCLDIPG
SGPWCPQNYTRKFYGEVTLARALRDSLNVPAVKVSEAVGRDKVRLVAGEFGIESDLADGPSLALGVSESTLLEMTGAYAG
ILNGGSSVTPYGVEALTLVGDSEPLMGASGGMGERVIRPVAAQELIWMMEKVIAEGTGQRAQIPGWQAAGKSGTTSSAKD
AWFIGFTADYVAGVWMGYDDNTPLTGVTGGGLPAEIWRETMVRVHDGLAPQPLPMAAPEPIVVPEPQPQRRNGGGFGREL
ERTLNGVLQDIFGN

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=569, Percent_Identity=32.3374340949033, Blast_Score=213, Evalue=5e-56,
Organism=Escherichia coli, GI1788867, Length=560, Percent_Identity=31.25, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87082258, Length=313, Percent_Identity=33.5463258785943, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1789601, Length=247, Percent_Identity=32.3886639676113, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79273; Mature: 79142

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQGRNSGRGTGRGRGPLVAEKRNLGGNQRKPAKKAPRKRAPQKAKRGWSLFGRRKTARP
CCCCCCCCCCCCCCCCCCEEEHHCCCCCCCCCHHHCHHHCCCHHHHCCHHHHCCCCCCCH
QRRRGLLGWLLLPFQIVARVIWAVSWRMGVVGGLFLAGAVAFHYTKLPEYQALLDGRHQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
SVTLLDRNGKTFAWRGDQFGGVVTADTVSPHLKNAIVATEDKRFYRHFGISPRGIASAVR
CEEEEECCCCEEEECCCCCCCEEEECCCCHHHHCCEEECCCHHHHHHCCCCCHHCCCEEE
INLSEGRGPLSGHGGSTITQQTAKLMCLGVEYDPDIWESEAAYESDCRQSSLWRKGKEAI
EEECCCCCCCCCCCCCHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FALAMEVKYTKNEILSIYMNRAYMGGGAHGVEAAAQRYFGKSANQVNPPEAAMLAGLLTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
PSTLAPTNNIERSRNRAATVLRLMEEQGYLSPKERQMAAANPAELSEAAAARAGGYFADW
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHH
IMDTVPSFLGDQTTEDVVIRTTLDQRLQQAAEDAVNYVFENKVRAGSKAQTAVVVMSADG
HHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
AVRAMVGGRKVRASGVFNRATQAKRQTGSAFKPFVYAAALELGYSPYDRVLDAEYCLDIP
CEEEHHCCCEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCHHEEECC
GSGPWCPQNYTRKFYGEVTLARALRDSLNVPAVKVSEAVGRDKVRLVAGEFGIESDLADG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCEEEEECCCCCCCCCCCC
PSLALGVSESTLLEMTGAYAGILNGGSSVTPYGVEALTLVGDSEPLMGASGGMGERVIRP
CCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
VAAQELIWMMEKVIAEGTGQRAQIPGWQAAGKSGTTSSAKDAWFIGFTADYVAGVWMGYD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHEEECCC
DNTPLTGVTGGGLPAEIWRETMVRVHDGLAPQPLPMAAPEPIVVPEPQPQRRNGGGFGRE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHH
LERTLNGVLQDIFGN
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQGRNSGRGTGRGRGPLVAEKRNLGGNQRKPAKKAPRKRAPQKAKRGWSLFGRRKTARP
CCCCCCCCCCCCCCCCCEEEHHCCCCCCCCCHHHCHHHCCCHHHHCCHHHHCCCCCCCH
QRRRGLLGWLLLPFQIVARVIWAVSWRMGVVGGLFLAGAVAFHYTKLPEYQALLDGRHQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
SVTLLDRNGKTFAWRGDQFGGVVTADTVSPHLKNAIVATEDKRFYRHFGISPRGIASAVR
CEEEEECCCCEEEECCCCCCCEEEECCCCHHHHCCEEECCCHHHHHHCCCCCHHCCCEEE
INLSEGRGPLSGHGGSTITQQTAKLMCLGVEYDPDIWESEAAYESDCRQSSLWRKGKEAI
EEECCCCCCCCCCCCCHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FALAMEVKYTKNEILSIYMNRAYMGGGAHGVEAAAQRYFGKSANQVNPPEAAMLAGLLTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
PSTLAPTNNIERSRNRAATVLRLMEEQGYLSPKERQMAAANPAELSEAAAARAGGYFADW
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHH
IMDTVPSFLGDQTTEDVVIRTTLDQRLQQAAEDAVNYVFENKVRAGSKAQTAVVVMSADG
HHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
AVRAMVGGRKVRASGVFNRATQAKRQTGSAFKPFVYAAALELGYSPYDRVLDAEYCLDIP
CEEEHHCCCEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCHHEEECC
GSGPWCPQNYTRKFYGEVTLARALRDSLNVPAVKVSEAVGRDKVRLVAGEFGIESDLADG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCEEEEECCCCCCCCCCCC
PSLALGVSESTLLEMTGAYAGILNGGSSVTPYGVEALTLVGDSEPLMGASGGMGERVIRP
CCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
VAAQELIWMMEKVIAEGTGQRAQIPGWQAAGKSGTTSSAKDAWFIGFTADYVAGVWMGYD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHEEECCC
DNTPLTGVTGGGLPAEIWRETMVRVHDGLAPQPLPMAAPEPIVVPEPQPQRRNGGGFGRE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHH
LERTLNGVLQDIFGN
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]