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The map label for this gene is hemF [H]

Identifier: 99082371

GI number: 99082371

Start: 2671697

End: 2672599

Strand: Reverse

Name: hemF [H]

Synonym: TM1040_2531

Alternate gene names: 99082371

Gene position: 2672599-2671697 (Counterclockwise)

Preceding gene: 99082372

Following gene: 99082370

Centisome position: 83.49

GC content: 59.91

Gene sequence:

>903_bases
ATGGCACAGAACACGGCAGAAATGCGTGACGAGAAGGCGCGCGCGGCGGCGTGGTTTCGAGAGCTGCGAGATCAGATCGT
TGCTGCATTCGAAGGGCTTGAGGACAGCCACCACGAAGGTCCCTTTACCGAGATGGGCGCCGGGCGGTTTGATGTGAGCG
ACACCAAACGCACCGCCGAGGATGGTTCCGACGCGGGTGGTGGCCTTATGAGCGTGATGCGTGGCGGGCGCGTTTTTGAA
AAGGTCGGTGTCAATATCTCCGAGGTCTACGGCACGCTGGGAGCACGCGCGCAACAGGCGATGGCGGCGCGCAAGGGGCT
GCCTGGCATGAAGGAAGATCCGCGCTTCTGGGCCTCTGGCATTTCCCTCGTGGCGCATATGCGCAATCCGCATGTGCCTG
CGGTTCACATGAACACCCGCATGTTCTGGACCCCTCATGGCTGGTGGTTCGGGGGTGGCTCAGACCTCAATCCCTGCATC
GAGTACGACGAGGATACGGCGCATTTCCATGAGGTGCAGCAGACGCATCTCGACCCCCATGGAGCGGATCTTTATCCCCG
CCTCAAGGCTTGGGCGGATGAGTATTTCTTTATTCCCCATCGCGGTCGGGCGCGGGGTGTTGGCGGGATCTTTCTGGATG
ACCACAACACGGGTGACTGGGCGCAGGATTTTGCATTGATTCAGGACATTGGCCGCGCCTTTTTGCCCGCCTTTTTGCCT
TTGGTGGAGCGGCGACGGGGGCATGCGTGCTCTGCGGATGACAAAGAGGCTCAGCTCATTCATCGCGGTCTCTATGCCGA
ATATAACCTCGTCTACGACCGCGGCACAAAATTTGGTCTCGAGACCGGGCACGATGCCAATGCAGTCCTGATGAGCCTGC
CTCCGCTGGCGAAATGGATTTGA

Upstream 100 bases:

>100_bases
GGTACTGACGCTCGACGGCGGGCGTACCCTGCTTGATCCTGTCACAGCGCCGGTGCATTGAGGCGGCAGTCTAAAACTCG
ATCTGATGAGGGAGGCCGTC

Downstream 100 bases:

>100_bases
TCGCGGCATAGGTGTAAATTCGCTCAATTGGCGTGAGCCCTGCGTAAATCTCGTATTCTGCGTTGAAGCACTGCTCTGTC
TCCGGCTCTATGGAGACAGG

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MAQNTAEMRDEKARAAAWFRELRDQIVAAFEGLEDSHHEGPFTEMGAGRFDVSDTKRTAEDGSDAGGGLMSVMRGGRVFE
KVGVNISEVYGTLGARAQQAMAARKGLPGMKEDPRFWASGISLVAHMRNPHVPAVHMNTRMFWTPHGWWFGGGSDLNPCI
EYDEDTAHFHEVQQTHLDPHGADLYPRLKAWADEYFFIPHRGRARGVGGIFLDDHNTGDWAQDFALIQDIGRAFLPAFLP
LVERRRGHACSADDKEAQLIHRGLYAEYNLVYDRGTKFGLETGHDANAVLMSLPPLAKWI

Sequences:

>Translated_300_residues
MAQNTAEMRDEKARAAAWFRELRDQIVAAFEGLEDSHHEGPFTEMGAGRFDVSDTKRTAEDGSDAGGGLMSVMRGGRVFE
KVGVNISEVYGTLGARAQQAMAARKGLPGMKEDPRFWASGISLVAHMRNPHVPAVHMNTRMFWTPHGWWFGGGSDLNPCI
EYDEDTAHFHEVQQTHLDPHGADLYPRLKAWADEYFFIPHRGRARGVGGIFLDDHNTGDWAQDFALIQDIGRAFLPAFLP
LVERRRGHACSADDKEAQLIHRGLYAEYNLVYDRGTKFGLETGHDANAVLMSLPPLAKWI
>Mature_299_residues
AQNTAEMRDEKARAAAWFRELRDQIVAAFEGLEDSHHEGPFTEMGAGRFDVSDTKRTAEDGSDAGGGLMSVMRGGRVFEK
VGVNISEVYGTLGARAQQAMAARKGLPGMKEDPRFWASGISLVAHMRNPHVPAVHMNTRMFWTPHGWWFGGGSDLNPCIE
YDEDTAHFHEVQQTHLDPHGADLYPRLKAWADEYFFIPHRGRARGVGGIFLDDHNTGDWAQDFALIQDIGRAFLPAFLPL
VERRRGHACSADDKEAQLIHRGLYAEYNLVYDRGTKFGLETGHDANAVLMSLPPLAKWI

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=243, Percent_Identity=44.0329218106996, Blast_Score=211, Evalue=5e-55,
Organism=Escherichia coli, GI1788777, Length=239, Percent_Identity=46.0251046025105, Blast_Score=213, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320249, Length=256, Percent_Identity=44.921875, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17864388, Length=243, Percent_Identity=44.8559670781893, Blast_Score=215, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 33489; Mature: 33358

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQNTAEMRDEKARAAAWFRELRDQIVAAFEGLEDSHHEGPFTEMGAGRFDVSDTKRTAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCHHHHC
DGSDAGGGLMSVMRGGRVFEKVGVNISEVYGTLGARAQQAMAARKGLPGMKEDPRFWASG
CCCCCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ISLVAHMRNPHVPAVHMNTRMFWTPHGWWFGGGSDLNPCIEYDEDTAHFHEVQQTHLDPH
HHHHHHHCCCCCCEEEECCEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
GADLYPRLKAWADEYFFIPHRGRARGVGGIFLDDHNTGDWAQDFALIQDIGRAFLPAFLP
CCCHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LVERRRGHACSADDKEAQLIHRGLYAEYNLVYDRGTKFGLETGHDANAVLMSLPPLAKWI
HHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEECCCHHHCC
>Mature Secondary Structure 
AQNTAEMRDEKARAAAWFRELRDQIVAAFEGLEDSHHEGPFTEMGAGRFDVSDTKRTAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCHHHHC
DGSDAGGGLMSVMRGGRVFEKVGVNISEVYGTLGARAQQAMAARKGLPGMKEDPRFWASG
CCCCCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ISLVAHMRNPHVPAVHMNTRMFWTPHGWWFGGGSDLNPCIEYDEDTAHFHEVQQTHLDPH
HHHHHHHCCCCCCEEEECCEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
GADLYPRLKAWADEYFFIPHRGRARGVGGIFLDDHNTGDWAQDFALIQDIGRAFLPAFLP
CCCHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LVERRRGHACSADDKEAQLIHRGLYAEYNLVYDRGTKFGLETGHDANAVLMSLPPLAKWI
HHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]