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The map label for this gene is pheS [H]

Identifier: 99082351

GI number: 99082351

Start: 2651928

End: 2653001

Strand: Reverse

Name: pheS [H]

Synonym: TM1040_2511

Alternate gene names: 99082351

Gene position: 2653001-2651928 (Counterclockwise)

Preceding gene: 99082353

Following gene: 99082350

Centisome position: 82.88

GC content: 61.36

Gene sequence:

>1074_bases
ATGGACGATCTTAAAGCAAAATACCTCGGCCAGATCGCCGAAGCCACGGATGAAAACGCGCTGGAGGCCGTGCGCCTCGC
GGCTGTGGGCAAGAAGGGCGAAGTCGCCCTCAAAATGCGTGAATTGGGCAAGATGACACCGGAAGAGCGCCAGGTGGCCG
GCCCGGCGCTGAATGCGCTCAAGGATGAGATCAACGCGGCGCTGGCGGCCAAGAAGGCAGCGCTTGGCGATGCGGCCCTG
GATGAGCGTCTGCGCTCGGAGTGGCTCGATGTGACGCTGCCAACGCGTCCGCGCCGCCAAGGGTCGATCCACCCGATTTC
TCAGGCGTCCGAGGAGCTGACTGCGATCTTTGCGGAAATGGGGTTCTCCGCTCAGGAAGGCCCGCGGGTTGATACCGACT
GGTACAACTTTGATGCGCTGAATATCCCCGGCCATCACCCGGCCCGTGCCGAGATGGACACGTTCTACATGCACCGCGCC
GAGGGCGATGATCGCCCGCCGCATGTACTGCGCACGCACACAAGCCCCGTTCAGATCCGCTCGATGGAGAAACTGGGCGC
GCCCTTGCGCATCATATGTCCCGGTGGCGTCTACCGCGCCGACTATGATCAGACCCACACCCCGATGTTCCATCAGGTCG
AAGGGCTGGCGCTGGACGAAAACATCTCCATGGCGAACCTCAAATGGGTTCTGGAGGAGTTCGTGAAGAGCTTCTTTGAA
GTGGACGAGGTTGAGCTGCGTTTCCGCGCGTCGCACTTCCCCTTCACGGAGCCCTCCGCAGAGGTCGACATCCGCTGCAG
CTGGGACAATGGTCAGCTGAAGATCGGCGAAGGCGACGACTGGCTTGAAATTCTCGGTTCCGGCATGGTGCATCCGAAGG
TGATCGCCGCTGGCGGGATCGACCCCGAAAAGTATCAAGGCTTTGCCTTTGGCATCGGGATCGACCGGCTGGCGATGCTG
AAATACGGAATCCCAGATCTGCGCGCCTTCTTTGAGAGCGACCTGCGCTGGTTGCGTCACTATGGCTTCAGCTGTCTGGA
CGTGCCGACACTGCATGGCGGCCTGAGCCGCTAA

Upstream 100 bases:

>100_bases
GCGACAGGAATGGGCTATGTCGTCGCTCCGGCGCCTCGGGTGCTTGCTTTCGACGCCTCGTGTGGTAGCAGGACATCAAA
GCGAGACTTGAGGGGCCGTC

Downstream 100 bases:

>100_bases
GTGTTTCTAATCGGCTTGTATCTGGCGCGGTACATCGACTGGTGTGCCGCGCTTTCGCGTAGAATGAGGCATTGGCAGAT
CCTTGTGATTGAGATGGCCT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MDDLKAKYLGQIAEATDENALEAVRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNALKDEINAALAAKKAALGDAAL
DERLRSEWLDVTLPTRPRRQGSIHPISQASEELTAIFAEMGFSAQEGPRVDTDWYNFDALNIPGHHPARAEMDTFYMHRA
EGDDRPPHVLRTHTSPVQIRSMEKLGAPLRIICPGGVYRADYDQTHTPMFHQVEGLALDENISMANLKWVLEEFVKSFFE
VDEVELRFRASHFPFTEPSAEVDIRCSWDNGQLKIGEGDDWLEILGSGMVHPKVIAAGGIDPEKYQGFAFGIGIDRLAML
KYGIPDLRAFFESDLRWLRHYGFSCLDVPTLHGGLSR

Sequences:

>Translated_357_residues
MDDLKAKYLGQIAEATDENALEAVRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNALKDEINAALAAKKAALGDAAL
DERLRSEWLDVTLPTRPRRQGSIHPISQASEELTAIFAEMGFSAQEGPRVDTDWYNFDALNIPGHHPARAEMDTFYMHRA
EGDDRPPHVLRTHTSPVQIRSMEKLGAPLRIICPGGVYRADYDQTHTPMFHQVEGLALDENISMANLKWVLEEFVKSFFE
VDEVELRFRASHFPFTEPSAEVDIRCSWDNGQLKIGEGDDWLEILGSGMVHPKVIAAGGIDPEKYQGFAFGIGIDRLAML
KYGIPDLRAFFESDLRWLRHYGFSCLDVPTLHGGLSR
>Mature_357_residues
MDDLKAKYLGQIAEATDENALEAVRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNALKDEINAALAAKKAALGDAAL
DERLRSEWLDVTLPTRPRRQGSIHPISQASEELTAIFAEMGFSAQEGPRVDTDWYNFDALNIPGHHPARAEMDTFYMHRA
EGDDRPPHVLRTHTSPVQIRSMEKLGAPLRIICPGGVYRADYDQTHTPMFHQVEGLALDENISMANLKWVLEEFVKSFFE
VDEVELRFRASHFPFTEPSAEVDIRCSWDNGQLKIGEGDDWLEILGSGMVHPKVIAAGGIDPEKYQGFAFGIGIDRLAML
KYGIPDLRAFFESDLRWLRHYGFSCLDVPTLHGGLSR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=30.4832713754647, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI5729820, Length=255, Percent_Identity=32.156862745098, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1788007, Length=341, Percent_Identity=50.1466275659824, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI32563657, Length=273, Percent_Identity=31.1355311355311, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=273, Percent_Identity=31.1355311355311, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=258, Percent_Identity=34.1085271317829, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=266, Percent_Identity=31.5789473684211, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=286, Percent_Identity=26.9230769230769, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI18858079, Length=272, Percent_Identity=30.1470588235294, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137424, Length=256, Percent_Identity=28.125, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39841; Mature: 39841

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLKAKYLGQIAEATDENALEAVRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNAL
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHH
KDEINAALAAKKAALGDAALDERLRSEWLDVTLPTRPRRQGSIHPISQASEELTAIFAEM
HHHHHHHHHHHHHHHCHHHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
GFSAQEGPRVDTDWYNFDALNIPGHHPARAEMDTFYMHRAEGDDRPPHVLRTHTSPVQIR
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHEEEECCCCCCCCCEEECCCCCHHHH
SMEKLGAPLRIICPGGVYRADYDQTHTPMFHQVEGLALDENISMANLKWVLEEFVKSFFE
HHHHHCCCEEEEECCCCEECCCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHC
VDEVELRFRASHFPFTEPSAEVDIRCSWDNGQLKIGEGDDWLEILGSGMVHPKVIAAGGI
CCHHHEEEEECCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHCCCCCCCEEEEECCC
DPEKYQGFAFGIGIDRLAMLKYGIPDLRAFFESDLRWLRHYGFSCLDVPTLHGGLSR
CHHHHCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MDDLKAKYLGQIAEATDENALEAVRLAAVGKKGEVALKMRELGKMTPEERQVAGPALNAL
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHH
KDEINAALAAKKAALGDAALDERLRSEWLDVTLPTRPRRQGSIHPISQASEELTAIFAEM
HHHHHHHHHHHHHHHCHHHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
GFSAQEGPRVDTDWYNFDALNIPGHHPARAEMDTFYMHRAEGDDRPPHVLRTHTSPVQIR
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHEEEECCCCCCCCCEEECCCCCHHHH
SMEKLGAPLRIICPGGVYRADYDQTHTPMFHQVEGLALDENISMANLKWVLEEFVKSFFE
HHHHHCCCEEEEECCCCEECCCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHC
VDEVELRFRASHFPFTEPSAEVDIRCSWDNGQLKIGEGDDWLEILGSGMVHPKVIAAGGI
CCHHHEEEEECCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHCCCCCCCEEEEECCC
DPEKYQGFAFGIGIDRLAMLKYGIPDLRAFFESDLRWLRHYGFSCLDVPTLHGGLSR
CHHHHCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA